925,693 research outputs found
Er Kişi Ömer Lütfi Mete: Conversation with Fatma Berra Mete
No full textYazar, şair, senarist, gazeteci, öğretmen başta olmak üzere birden fazla meslek ve nitelik ile kendisinden söz ettiren Ömer Lütfi Mete, 1 Şubat 1950 tarihinde Rize’nin İyidere ilçesinde dünyaya gelmiştir. Mete, ilkokul ve ortaokulu İyidere’de okumuş, lise eğitimini ise Rize Lisesi’nde almıştır. Bunun yanı sıra çocukluk yıllarında ve gençliğinin ilk dönemlerinde çeşitli Kur’an kurslarında dini eğitim görmüştür. 1970 yılında İstanbul Üniversitesi İktisat Fakültesi’nde üniversiteye başlamış ancak bu öğrenimini yarıda bırakmak durumunda kalmıştır. Bu yıllarda gazeteciliğe adım atan Mete, 1974 yılında Marmara Üniversitesi Eğitim Fakültesi’nin Türkçe bölümünde yeniden üniversiteye dönmüştür. Mezun olduktan sonra Rize Lisesi’nde bir süre edebiyat öğretmenliği yapmış sonrasında ise çeşitli gazete ve dergilerde yazarlık ve yöneticilik yapmaya başlamıştır. 1978 yılından itibaren roman, deneme, hikâye ve şiir türünde başarılı eserler kaleme almıştır. 1990’lı yıllarda televizyon sektöründe yer almaya başlayan Mete, ilgiyle izlenen dizi ve filmlerin senaristliğini ve danışmanlığını yapmasının yanı sıra çeşitli televizyon programlarında yorumculuk görevini üstlenmiştir. Gerek eserlerinde gerekse konuşmalarında dini ve milli değerleri daima savunan, sürekli iyiliğe ve doğruluğa vurgu yapan Mete, Türk düşünce dünyasında kalıcı iz bırakmış, değerli bir mütefekkirdir. 18 Kasım 2009 tarihinde vefat eden Mete, ardında onlarca değerli eser bırakmıştır. Söz konusu bu çalışma Ömer Lütfi Mete’nin gönül ve zihin dünyasını anlamaya katkı yapması gayesiyle hazırlanmıştır.Ömer Lütfi Mete, who made a name for himself in multiple professions and qualifications, including as a writer, poet, scriptwriter, journalist and teacher, was born on 1 February 1950 in İyidere district of Rize. Mete went to primary and secondary school in İyidere and high school at Rize High School. Besides, he had a religious education in the various Quran courses during his childhood and early youth. In 1970 he began university education at the Istanbul University Faculty of Economics. However, he had to drop out of his education there. Mete, who stepped into journalism during these years, returned to university in the Turkish department of Marmara University Faculty of Education in 1974. After graduation, he worked as a teacher at Rize High School briefly. After that, he started to work as a writer and manager in various magazines and newspapers. Since 1978, he has written successful works in the genres of novel, story, essay and poetry. Mete, who started to work as a scriptwriter and consultant for popular movies and TV series, as well as a commentator for various TV programs. Mete, who always defended religious and national values, emphasized goodness and rightousness both in his works and speeches, is valuable thinker, who left a mark in the Turkish World of Thought. He died on 18 November 2009, leaving behind many precious works. This study has been prepared to contribute to the understanding of Ömer Lütfi Mete’s world of heart and mind
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Nuh Mete Yüksel'in hayatı
No full textAnkara : İhsan Doğramacı Bilkent Üniversitesi İktisadi, İdari ve Sosyal Bilimler Fakültesi, Tarih Bölümü, 2019.This work is a student project of the Department of History, Faculty of Economics, Administrative and Social Sciences, İhsan Doğramacı Bilkent University.The History of Turkey course (HIST200) is a requirement for all Bilkent undergraduates. It is designed to encourage students to work in groups on projects concerning any topic of their choice that relates to the history of Turkey. It is designed as an interactive course with an emphasis on research and the objective of investigating events, chronologically short historical periods, as well as historic representations. Students from all departments prepare and present final projects for examination by a committee, with 10 projects chosen to receive awards.Includes bibliographical references (page 19).Nuh mete yüksel 41 yıllık savcılık hayatına Ankara hukuk fakültesinden mezun olduktan sonra başlamıştır. Bu araştırmada Nuh Mete Yükselin “Nuh Mete Yüksel Anlatıyor ve “Nuhun Gemisi” kitaplarından ve gazete haberlerinden yararlanılarak özel hayatı ve meslek hayatı incelenmiştir. Ayrıca kendisiyle yaptığımız röportajda, özel hayatıyla ilgile çok bilinmeyen ve başka hiçbir mecrada yer almayan bilgileri ve içinde bulunduğu davalardaki bazı detayları vermiştir.Nuh Mete Yüksel has been a prosecutor for 41 years and he started his job after his graduation from Ankara Law School. In this research, two of Nuh Mete Yüksel’s book “Nuh Mete Yüksel Anlatıyor” and “Nuh’un Gemisi" and newspaper articles were used to examine his private and professional life. Also, in the personal interviews with him, he talked about his unfamiliar personal life that had no place in anywhere and he gave some details about the cases which he took place in.by İbrahim Mert Öztürk
STRATEGI PEMASARAN METE SUPER WONOGIRI BERDASARKAN ANALISIS SWOT (Studi Kasus Sentra Jambu Mete di Kecamatan Jatisrono, Kabupaten Wonogiri)
No full textThe purposes of this research were to know: (1) the current condition of Mete Super Jatisrono based on SWOT analysis and the right strategy to develop Mete Super Jatisrono based on SWOT anaysis The method used in this research was a qualitative method with SWOT analysis as the main method. The researcher used documentation, field observation and quisioner as the data collection techniques. The researcher was able to determine the current condition of Mete Super Jatisrono by identity the internal and external factors. The results of this research were: S-O strategies was the most suitable strategies that can be used as main strategy to develop Mete Super Jatisrono. There is two main poin contained on S-O strategies: the first one is by doing online promotion by themself without hiring a social media expert, and the second one is by doing endorsement to promote Mete Super Jatisrono.
Key Words: SWOT analysis, Mete Super Jatisron
Cloning and expression of the metE gene in Escherichia coli
No full textA −transducing phage was isolated that contains the metE gene. This gene codes for N5−methyl−H4−folate:homocysteine methyltransferase (EC 2.1.1.14), an enzyme that catalyzes the terminal reaction in methionine biosynthesis. A 9.1−kb EcoR1 fragment of this phage, containing the metE gene, was then cloned into pBR325. This plasmid, pJ19, was used to transform Escherichia coli strain 2276, a metE mutant, and restore the MetE+ phenotype. Although the transformed cells produced large amounts of the metE protein in vivo, in vitro studies using pJ19 as template showed low synthesis of the metE protei
VKS-METE - Ura, William Mete, Dillon's Bay Erromango
No full textJanuary 24th, 1978 Recordings of Ura Language: Recorded by William Mete at Dillon's Bay Erromango Recordings of men and women believed to be the last speakers on on the island of Ura.. Language as given
The Equilibrium Constant for MetE Oxidation Is Consistent with MetE Inactivation In Vivo
No full text<div><p>(A) MetE was incubated with the indicated [GSH]/[GSSG] ratios at 1.1 mM (yellow), 2.3 mM (red), 4.6 mM (green), or 9.1 mM (blue) GSH until equilibrium was reached, as judged by a constant level of activity with time. MetE activity was then assayed to determine the relative amount of active reduced enzyme. The equilibrium constant, K<sub>mix</sub> (see <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.0020336#pbio-0020336-e002" target="_blank">equation 2</a>) was determined to be 1.4 from the plot of relative MetE activity versus [GSH]/[GSSG]. This value was independent of the GSH concentration and dependent only on the [GSH]/[GSSG] ratio, consistent with glutathionylation as the mechanism of inactivation.</p>
<p>(B) Cellular redox potentials are often referenced in terms of the GSH–GSSG couple, which depends upon [GSH]<sup>2</sup>/[GSSG]. Since inactivation of MetE occurs via glutathionylation (which is dependent on [GSH]/[GSSG]), a redox potential (which is dependent on [GSH]<sup>2</sup>/[GSSG]) for inactivation of MetE (by equilibrium titration with GSH/GSSG) cannot be determined. In order to provide a physiological context for the K<sub>mix</sub> determined in vitro, the equilibrium fraction of MetE expected to be active at different glutathione concentrations, 1 mM (red), 2 mM (green), and 5 mM (blue), was determined as a function of the cellular potential as described in the text. The regions designated “normal growth” and “oxidative stress” were inferred from published estimates of typical redox potentials in cells experiencing these conditions (<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.0020336#pbio-0020336-Gilbert1" target="_blank">Gilbert 1990</a>).</p></div
MetE: a promising protective antigen for tuberculosis vaccine development
No full textIntroduction: Tuberculosis (TB), caused by Mycobacterium tuberculosis (MTB), remains a significant global health concern. The existing vaccine, Bacillus Calmette-Guérin (BCG), provides inconsistent protection, highlighting the pressing need for a more effective vaccine. We aimed to identify novel MTB antigens and assess their protective efficacy as TB vaccine candidates.
Methods: Using immunopeptidomics, we identified 64 and 80 unique mycobacterial antigens derived from BCG and MTB, respectively. We prioritised antigens based on HLA allele coverage through an immunoinformatics approach.
Results: The candidates, hisD, metE, and mmpL12, delivered as DNA vaccines, were evaluated for efficacy in mice using the ex vivo Mycobacterial Growth Inhibition Assay (MGIA) and metE was identified as a promising candidate. In vivo murine MTB challenge experiments confirmed the protective efficacy conferred by metE when formulated as recombinant protein with AS01™ or AddaS03™ adjuvants, compared to the naïve group. The immunogenic profiles of metE formulated in the two different adjuvants differed, with metE-AS01™ inducing antigen-specific IFN-γ, TNF-α, IL-2, IL-17, IgG1 and IgG2a-c, while metE-AddaS03™ induced TNF-α, IL-2, IL-17, IL-4, IgM, IgG1, IgG2b.
Conclusion: Our findings highlight metE as a promising protective antigen for future TB vaccine development
IbM PEMBERDAYAAN PETANI JAMBU METE BERBASIS ENTREPRENEURSHIP
No full textThe Farmer Group TUNAS MAJU is an organization founded in 2007 and serves to facilitate the cashew farming communities in North Lombok. Farmers only use cashew seeds for sale, while the cashew fruit is not used optimally by farmers, even wasted by useless as waste. Lack of knowledge of farmers become obstacles in managing the cashew fruit, but fruit can be processed into Nata de Mete. Therefore, IbM program is to empower people to cultivate Nata de Mete and Nata de Mete be further processed into a beverage packaging. IbM program has been carried out with the result of performance: (1) a site survey; (2) coordinate with the farmer group chairman TUNAS MAJU and Mangala Village Chief; (3) extension of cashew farmers conducted twice (4) Preparation of training the manufacture of Nata de Mete. (5) The training was conducted by three techniques, namely teaching; Demonstrations; Practice. (6) Assistance carried out by means of direct assistance and consultation. (7) The product produced in this training is Nata de Mete with characteristics that thickness ± 08 to 1.8 cm; chewy texture; rather pale white color. Beverage Packaging While that feels good and fresh
Identification of Rv1133c (MetE) as a marker of Mycobacterium tuberculosis replication and as a highly immunogenic antigen with potential immunodiagnostic power
No full textThe immunization of mice with the sterile culture medium supernatants of Mycobacterium tuberculosis (Mtb) H37Rv permitted the production of several monoclonal antibodies (mAbs) specific for secreted and/or released antigens. Two mAbs bound and immunoprecipitated an 80-kDa protein that was identified by mass spectrometry as Rv1133c, the methionine synthase MetE. The protein MetE is ubiquitous among prokaryota and shows a significant sequence homology in many bacteria. We produced both the full-length recombinant MetE and its N-terminal fragment, whose sequence is more conserved among mycobacteria, to select mAbs recognizing an Mtb-specific region of MetE. Finally, we produced and selected eight mAbs that specifically detect the MetE protein in the supernatant and cell lysate of Mtb and BCG, but not other bacteria such as non-tuberculous mycobacteria (NTM), Streptococcus pneumoniae, Staphylococcus aureus, Acinetobacter baumanii, or Escherichia coli. Taking advantage of our mAbs, we studied (i) the vitamin B12 dependence for the synthesis of MetE in Mtb and NTM and (ii) the kinetics of MetE production and secretion in supernatants during the in vitro reproduced replicative, dormant, and resuscitation cycle of Mtb. Our data demonstrate that dormant Mtb, which are assumed to be prevalent in latent infections, as well as NTM do not produce and secrete MetE. Results indicate an unexpected specificity for Mtb of our anti-MetE mAbs and encourage the use of rMetE and our mAbs as tools for the immunodiagnosis of TB and its stages
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