34 research outputs found
Trichoderma‐Derived Pentapeptides from the Infected Nest Mycobiome of the Subterranean Termite Coptotermes testaceus
No full textTermites live in a dynamic environment where colony health is strongly influenced by surrounding microbes. However, little is known about the mycobiomes of lower termites and their nests, and how these change in response to disease. Here we compared the individual and nest mycobiomes of a healthy subterranean termite colony (Coptotermes testaceus) to one infected and ultimately eradicated by a fungal pathogen. We identified Trichoderma species in the materials of both nests, but they were also abundant in the infected termites. Methanolic extracts of Trichoderma sp. FHG000531, isolated from the infected nest, were screened for secondary metabolites by UHPLC‐HR MS/MS‐guided molecular networking. We identified many bioactive compounds with potential roles in the eradication of the infected colony, as well as a cluster of six unknown peptides. The novel peptide FE011 was isolated and characterized by NMR spectroscopy. The function of this novel peptide family as well as the role of Trichoderma species in dying termite colonies therefore requires further investigation
The positive effect of plant diversity on soil carbon depends on climate
No full textLittle is currently known about how climate modulates the relationship between plant diversity and soil organic carbon and the mechanisms involved. Yet, this knowledge is of crucial importance in times of climate change and biodiversity loss. Here, we show that plant diversity is positively correlated with soil carbon content and soil carbon-to-nitrogen ratio across 84 grasslands on six continents that span wide climate gradients. The relationships between plant diversity and soil carbon as well as plant diversity and soil organic matter quality (carbon-to-nitrogen ratio) are particularly strong in warm and arid climates. While plant biomass is positively correlated with soil carbon, plant biomass is not significantly correlated with plant diversity. Our results indicate that plant diversity influences soil carbon storage not via the quantity of organic matter (plant biomass) inputs to soil, but through the quality of organic matter. The study implies that ecosystem management that restores plant diversity likely enhances soil carbon sequestration, particularly in warm and arid climates.This article is published as Spohn, M., Bagchi, S., Biederman, L.A. et al. The positive effect of plant diversity on soil carbon depends on climate. Nat Commun 14, 6624 (2023). https://doi.org/10.1038/s41467-023-42340-0. © The Author(s) 2023.This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Identification of a novel aminopolycarboxylic acid siderophore gene cluster encoding the biosynthesis of ethylenediaminesuccinic acid hydroxyarginine (EDHA)
No full textSpohn M, Edenhart S, Alanjary M, et al. Identification of a novel aminopolycarboxylic acid siderophore gene cluster encoding the biosynthesis of ethylenediaminesuccinic acid hydroxyarginine (EDHA). METALLOMICS. 2018;10(5):722-734.The mechanism of siderophore-mediated iron supply enhances fitness and survivability of microorganisms under iron limited growth conditions. One class of naturally occurring ionophores is the small aminopolycarboxylic acids (APCAs). Although they are structurally related to the most famous anthropogenic chelating agent, ethylenediaminetetraacetate (EDTA), they have been largely neglected by the scientific community. Here, we demonstrate the detection of APCA gene clusters by a computational screening of a nucleotide database. This genome mining approach enabled the discovery of a yet unknown APCA gene cluster in well-described actinobacterial strains, either known for their potential to produce valuable secondary metabolites (Streptomyces avermitilis) or for their pathogenic lifestyle (Streptomyces scabies, Corynebacterium pseudotuberculosis, Corynebacterium ulcerans and Nocardia brasiliensis). The herein identified gene cluster was shown to encode the biosynthesis of APCA, ethylenediaminesuccinic acid hydroxyarginine (EDHA). Detailed and comparatively performed production and transcriptional profiling of EDHA and its biosynthesis genes showed strict iron-responsive biosynthesis
Complete genome sequence of the actinobacterium Amycolatopsis japonica MG417-CF17(T) (=DSM 44213T) producing (S,S)-N,N '-ethylenediaminedisuccinic acid
No full textComplete genome sequence of the actinobacterium Amycolatopsis japonica MG417-CF17T (=DSM 44213T) producing (S,S)-N,N′-ethylenediaminedisuccinic acid
No full textWe report the complete genome sequence of Amycolatopsis japonica MG417-CF17T (=DSM 44213T) which was identified as the producer of (S,S)-N,N′-ethylenediaminedisuccinic acid during a screening for phospholipase C inhibitors. The genome of A. japonica MG417-CF17T consists of two replicons: the chromosome (8,961,318bp, 68.89% G+C content) and the plasmid pAmyja1 (92,539bp, 68.23% G+C content), encoding a total of 8422 protein coding genes. Analysis of the sequence data revealed 30 clusters encoding the biosynthesis of secondary metabolites
Preemption and a Dilemma for Causal Decision Theory
No full textThis item has been retired at the request of its author
"It's not all cupcakes and lollipops": An investigation of the predictors and effects of prison visitation for children during maternal and paternal incarceration
No full textabstract: The purpose of this project is to better understand the factors associated with, and effects of, prison visitation for children during maternal and paternal incarceration. As gatekeepers, caregivers play a pivotal role in the facilitation of parent-child prison visitation. Yet, some caregivers may be more likely to take children to visit than others. Additionally, among those children who do visit, visitation may be positive in some ways and negative in others. To advance prior work, this study (1) assesses the relationship between caregiver type and parent-child prison visitation and (2) investigates the emotional and behavioral responses of children who visit. The current research uses mixed-methods and is carried out in two phases. For Phase 1, quantitative data on 984 children collected from structured interviews with incarcerated parents (N=279 mothers; N=143 fathers) in the Arizona Department of Corrections are used to examine the relationship between caregiver type and the likelihood of parent-child prison visitation. Descriptive statistics and logistic regression analyses are conducted separately for maternal and paternal incarceration. Phase 2 draws on caregivers' accounts of 40 children who visit their parent in prison to assess children's emotional and behavioral reactions to visitation. Data are coded to identify positive and negative responses, "visitation paradox" indicators, prior life circumstances and child age. Thematic content analyses are conducted to capture major themes. Analyses from Phase 1 confirm a significant relationship between caregiver type and mother-child and father-child visitation. Other factors that affected the likelihood of parental visitation included child situational factors, parent stressors, institutional barriers and child demographics, although these effects differed depending upon which parent was in prison. Results from Phase 2 revealed overwhelmingly negative responses among children to parental prison visitation. Key themes that accounted for child reactions included institutional context and parental attachment. This research adds to the collateral consequences of incarceration literature by providing greater insight into the imprisonment experience for vulnerable families. Further, these results have direct implications for correctional policy and practice pertaining to the manner and regulation of prison visits and also inform reentry efforts through a family-centric approach.Dissertation/ThesisPh.D. Criminology and Criminal Justice 201
Bioactive natural products from Bacteroidetes
No full text10451065Bacteria representing the phylum Bacteroidetes produce a diverse range of natural products, including polyketides, peptides and lactams. Here, we discuss unique aspects of the bioactive compounds discovered thus far, and the corresponding biosynthetic pathways if known, providing a comprehensive overview of the Bacteroidetes as a natural product reservoir.39
Assessing Disparity in the Federal Court Processing of Immigration Cases
No full textabstract: In recent decades, the United States has experienced a wave of immigration, an economic recession, and several terroristic attacks. In response, the government has scapegoated and blamed undocumented immigrants of color for recent social ills. As a result, a large share of government resources has been allocated to the enforcement and processing of immigration violations. Consequently, the number of immigration cases processed in U.S. federal courts has spiraled to nearly 50% of bookings and 34% of federal sentencing cases. Yet, immigration offenses have received little empirical attention in the courts and sentencing literature due in part to differences in the way immigration offenses are processed compared to other federal offense types, and relatedly, the empirical difficulties immigration offenses pose for analysis. Nevertheless, the increased representation of immigration offenses in federal courts, along with the punitive rhetoric and heightened social control targeting undocumented immigrants of color, warrants a comprehensive assessment of how immigration cases are processed in U.S. federal courts. Accordingly, this dissertation seeks to identify inequality in the processing of immigration cases by examining: 1) cumulative disadvantage within immigration cases; 2) contextual disparity and how social context interacts with ethnicity to influence multiple federal court outcomes within immigration cases; and 3) ethnic disparity within immigration cases over time.
Data come from the Federal Justice Statistics Program Data Series, the U.S. Census, the Uniform Crime Reports, Leip’s Atlas of U.S. Presidential Elections, the National Judicial Center, and the U.S. Department of Justice. The quantitative analysis addresses the first question by employing a cumulative disadvantage approach where multiple decision points are considered and the effects of prior stages on subsequent outcomes. The quantitative analysis proceeds to address the second question by using multilevel modeling for multiple court outcomes. The longitudinal analysis is separately conducted on sentence length for 18-year data, from 1994 through 2012, to assess racial and ethnic disparity over time.
The results indicate that cumulative disadvantage is present within immigration cases, that social context influences certain decision points, and that ethnic disparity has diminished over time in some districts.Dissertation/ThesisDoctoral Dissertation Criminology and Criminal Justice 201
Procedural Justice, Veteran Identity and Legal Legitimacy in Veteran Treatment Courts
No full textabstract: In the wake of the wars in Iraq and Afghanistan, courts and social service systems across the country have begun establishing veterans treatment courts (VTC). The first VTC was created in 2004 and there are now over 300 in at least 35 states. Yet, their underlying assumptions have not been clearly articulated and their functioning and outcomes have not been well tested. These courts aim to reduce rates of incarceration and recidivism among justice-involved veterans and draw heavily on the structure and assumptions of drug and mental health courts. However, VTCs are different in important ways. Unlike other problem solving courts, VTCs actively express gratitude to criminal defendants (for past military service) and have the ability to connect participants to a socially-esteemed identity. Earlier problem solving courts have drawn on Tyler’s theory of procedural justice to predict a path from procedurally fair treatment and social bonds with court personnel through changes in social identity to increased perceptions of legal legitimacy and, ultimately, program completion and reduced recidivism. The present study tested a modified, version of Tyler’s theory that incorporates gratitude and focuses on veteran identity as the mediating construct between fair treatment and perceptions of legal legitimacy. A cross-sectional survey design was used with a convenience sample (N = 188) of participants in two Arizona VTCs. The results indicate that perceptions of procedural justice, perceived social bonds and receipt of gratitude are positively associated with both veteran identity and perceptions of legal legitimacy. Further, veteran identity was found to be a significant mediator between the first three constructs and legal legitimacy. Finally, neither recidivism risk nor race/ethnicity moderated the relationships. The study supports the importance of acknowledging past military service and enhancing the level of veteran identity among VTC participants. Implications for practice and future research are discussed.Dissertation/ThesisDoctoral Dissertation Social Work 201
