5,552 research outputs found
Appunti in tema di affidamento fiduciario successorio, in onore e memoria di Carlo Maria Barone
No full textScrivere di cose giuridiche: l'attualità del contributo di Carlo Maria Barone
No full textUna riflessione intorno allo stato dell'informazione giuridica e un tributo a uno studioso, non accademico, di grandissima levatura
Coeliac Disease, a model of food-induced inflammatory disease
No full textCoeliac Disease, a model of food-induced inflammatory disease
Celiachia, un modello di patologia infiammatoria indo:a da alimen;
M. Vi&oria Barone
Background/aim.
Epidemiological studies, have associated the increase in chronic inflammatory diseases, such as diabetes, atherosclerosis, asthma, chronic liver disease, autoimmune diseases, degenerative diseases and inflammatory bowel diseases to the spread of the so-called " Western diet”. The Mediterranean diet has increasingly been regarded as the gold-stantard diet for human health.
The nutrients exert their effects on tissue inflammation, either by direct action on the cells, or because they regulate the composition of the intestinal microbiota. The enterocyte and the intestinal immunocompetent cells, are equipped with complex systems to "feel" the food and to respond to them. Even cereals (and in particular wheat) can cause intestinal inflammation, for example in celiac disease. The intestinal damage from gluten (the complex of soluble alcohol proteins in wheat) in celiac disease consists of inflammation and remodelling of the mucosa, with flattening of the villi and hypertrophy of the crypts. There are two main types of inflammatory response to gluten peptides in celiac disease (1): there is the adaptive response mediated by CD4 + T cells to some peptides: the 33 mer of A-Gliadin, resistant to gastric digestion, is a prototype endoluminal and parietal intestinal. The peptide is deamidated by tissue transglutaminase (tTG) and presented by class II histocompatibility antigens, DQ2 and DQ8, to CD4 + T cells, with Th1-type response, mediated by gamma interferon and other proinflammatory cytokines. Then there is the response, not mediated by T cells, to other peptides: the
peptide 31-43 of A-gliadin (P 31-43) is the prototype. P 31-43, which is part of peptide 31-55, is resistant to gastric and intestinal digestion and causes inflammation with multiple mechanisms, the best known of which consists of a stress / innate and proliferative response, mediated by EGF and IL15 (2). Peptide P31-43 is also a growth factor for various cell lines and for the enterocyte of celiac, as it is able to activate the EGF-EGFR system, the most powerful mitogen present in our body. The gliadin-induced proliferation of celiac crypt is not only dependent on EGF but also on IL15. The proliferation of crypt enterocytes and the innate immune response to celiac gliadin are regulated by a cooperation between EGF and IL15. P31-43 induce also a stress/innate immune response involving interferon α (IFN-α) (3). Thus a double action of gliadin is delineated in the induction of the mucosal damage of celiac subjects. On the one hand, gliadin can activate the T-mediated response and on the other it can induce an innate/ inflammatory response. The combined effects of this double action induce the typical lesion of the celiac mucosa. But what causes the sensitivity to gliadin peptides in celiac cells is not really clear.
In this study, we describe a stressed/inflamed celiac cellular phenotype in enterocytes and fibroblasts probably due to an alteration in the early-recycling endosomal system. Moreover we show that celiac cells are more sensitive to the gliadin peptide P31-43 and IL15 than controls. This phenotype is reproduced in control cells by inducing a delay in early vesicular trafficking.
Methods. For organ culture studies, biopsy fragments from duodenum were obtained from CD patients with villous atrophy controls, affected by gastroesophageal reflux, and CD patients on GFD (Gluten free diet). Fibroblasts were cultured from skin and intestinal biopsies obtained from CD patients, (GFD, GCD) and controls. We used double immunofluorescence staining, western blotting and immunoprecipitation to evaluate EGFR and EEA1 levels and co- localization in fibroblasts and biopsy fragment from patients with CD and controls. We transfected cells with siHRS and mRNA analysis was
perfomed to evaluate the levels of EEA1.
Results. We found in CD biopsies and fibroblasts an increase of markers of the innate immune response (EGFR, IL15-R α, MXA) and of the inflammatory response (NFkB). CD cells (enterocytes and fibroblasts) presented a constitutive alteration in the intracellular vesicular system at the level of the early-recycling compartment. In fact, in CD enterocytes and fibroblasts, the numbers of early vesicles were increased, and the EGF/EGFR trafficking was delayed in the early endocytic vesicles; moreover, the decay of the EGFR was prolonged, and TfR levels were increased. We induced a delay in early endocytic trafficking by transfecting cells with siHRS in control cells and rendered them more sensitive to gliadin treatment using as read out STAT5 and NFkB levels.
Conclusions. In the present study we show that cells from celiac disease patients present a delay of the endocytic trafficking and are more sensitive to the wheat gliadin peptide P31-43 than control cells. We also found that inducing delays in early vesicular trafficking leads to a celiac-like cellular phenotype, carachterized by inflammation and activation of innate immunity, thus implicating the early-recycling endosomal system in celiac disease. This constitutive lesion might mediate the stress/innate immune response to gliadin, which in turn triggers the gliadin-specific T-cell response.
1) Barone MV, Troncone R, Auricchio S.Gliadin peptides as triggers of the proliferative and stress/ innate immune response of the celiac small intestinal mucosa. Int J Mol Sci. 2014 15(11):20518-37.
2) Barone MV, Zimmer KP. Endocytosis and transcytosis of gliadin peptides. Mol Cell Pediatr. 2016
3:8.
3) Nanayakkara M, Lania G, Maglio M, Auricchio R, De Musis C, Discepolo V, Miele E, Jabri B,
Troncone R, Auricchio S, Barone MV.P31-43, an undigested gliadin peptide, mimics and enhances the innate immune response to viruses and interferes with endocytic trafficking: a role in celiac disease.Sci Rep. 2018 8:1082
BIOGRAFIA Mario De Maria
No full textMario de Maria nacque a Bologna il 9 settembre del 1852. Sia il padre, il barone Fabio de Maria, medico chirurgo, che la madre erano di nobili origini. Mario trascorse la sua adolescenza a Bologna tra la musica e l'arte. Il padre, direttore d'orchestra, amava inoltre disegnare e dipingere
Enzo Fortuna: i progetti di Restauro e allestimento per il Museo di Palazzo Bellomo a Siracusa 1952-1970
No full textIl contributo espone l'importanza di una figura come quella dell'architetto Enzo Fortuna all'interno del progetto di restauro e allestimento (1952-1970) che realizza per il museo di palazzo Bellomo a Siracusa. I documenti dell'architetto sono stati oggetto di una mostra ospitata a Palazzo Bellomo dal 18 giugno al 18 luglio 2013, curata da M.R. Vitale (associato ICAR/19, Università di Catania) e Zaira Barone (dottore di ricerca icar/19). Titolo della mostra: Enzo Fortuna: i progetti per Palazzo Bellomo, 1952-1970 (Siracusa, Palazzo Bellomo 2013), nell’ambito della “III Giornata Nazionale Archivi di Architettura.
Dall’archivio al museo”, promossa da AAA Italia, con il sostegno della Direzione generale per gli Archivi, Galleria Regionale di Palazzo Bellomo e Direzione generale per il paesaggio, le belle arti, l’architettura e l’arte contemporanea, il patrocinio dell’ICOM e della Galleria Regionale di Palazzo Bellomo
Vivere il diritto. Studi in onore di Carlo Maria Barone
No full textRaccolta di scritti (31) dedicati alla memoria di Carlo Maria Baron
Building a supportive learning environment in pre-school based on Maria Montessori`s guiding principles
No full textKvalifikācijas darba autore: Evita Rasnača Kvalifikācijas darba vadītāja: Mg. edu - Inese Barone Kvalifikācijas darba temats: “Atbalstošas mācību vides veidošana pirmsskolā pēc Marijas Montesori pamatprincipiem”. Kvalifikācijas darba mērķis: Teorētiski pārbaudīt un empīriski analizēt mācību vidi pēc Marijas Montesori pamatprincipiem pirmsskolā. Kvalifikācijas darbs tiek izstrādāts divās daļās. Pirmajā daļā tiek izpētīta un analizēta teorētiskā literatūra par to, kas ir mācību vide. Otrajā daļā tiek veikta anketēšana pirmsskolas izglītības iestādes pedagogiem. Kvalifikācijas darba izstrādes procesā tika veikta anketu analīze. Kvalifikācijas darba nobeigumā ir secinājumi un priekšlikumi. Kvalifikācijas darba apjoms ir 38 lappuses. Tajā ir iekļautas 2 tabulas, 11 attēli un 4 pielikumi. Izmantoti ir 23 literatūras avoti. Atslēgas vārdi: vide, mācību vide, Montesori.The author of the qualification paper: Evita Rasnača Qualification Supervisor: Mg. edu - Inese Barone The topic of the qualification paper: “Building a supportive learning environment in pre-school based on Maria's Montessori's guiding principles”. Purpose of the qualification paper: Theoretically test and empirically analyse the learning environment on the basis of Maria Montesori basic principles in pre – school. The qualification work is being developed in two parts. The first part explores and analyses theoretical literature about what a learning environment is. In the second part, a questionnaire is carried out for teachers of the pre – school educational institution. Questionnaire analysis was carried out during the qualification work development process. The qualification work concludes with conclusions and proposals. The size of the qualification paper is 38 pages. It includes 2 tables, 11 images and 4 attachments. There are 23 literary sources used. Keywords: environment, learning environment, Montesori
La chiesa di Santa Maria di Campogrosso ad Altavilla Milicia (Palermo), i ruderi di un monumento normanno tra abbandono, restauri e studi archeologici
No full textThe church of Santa Maria di Campogrosso, abandoned for some time, is now in ruins. However, this church represents an important historical and architectural witness of the county period in Sicily. In addition, the story of this monument is linked to a very important episode, the Norman conquest of Sicily with the emergence of the so-called “Arab-Norman” architecture.
The essay describes the history of the church between abandonment, restoration and archaeological studies up to the presen
Ex-voto del Barone Felice Pastore
No full textEx-voto donato alla Madonna dei Miracoli di Alcamo da Felice Pastore, Barone di Rincione (1789-1862) costituito da manufatti aurei tipici dell'oreficeria siciliana della seconda metà del XIX secolo
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