33 research outputs found
A Concise Ring Closing Enyne Metathesis Approach for the Preparation of Functionalized Proline‐Derived Azabicycloalkane Amino Acids
No full textHerein we report the preparation of C4-functionalized proline-derived azabicycloalkane (Aba) amino acids by exploiting a direct ring closing enyne metathesis reaction of readily accessible dipeptide precursors. The reverse-turn inducing properties of the newly synthesized constrained dipeptide mimics were assessed by computational studies. Furthermore, the desired Aba derivatives, carrying a synthetically versatile diene moiety, were subjected to post-functionalization reactions to validate their potential application in peptide/peptidomimetic chemistry and drug discovery
Glycovaccine Design: Optimization of Model and Antitubercular Carrier Glycosylation via Disuccinimidyl Homobifunctional Linker
No full textConjugation via disuccinimidyl homobifunctional linkers is reported in the literature as a convenient approach for the synthesis of glycoconjugate vaccines. However, the high tendency for hydrolysis of disuccinimidyl linkers hampers their extensive purification, which unavoidably results in side-reactions and non-pure glycoconjugates. In this paper, conjugation of 3-aminopropyl saccharides via disuccinimidyl glutarate (DSG) was exploited for the synthesis of glycoconjugates. A model protein, ribonuclease A (RNase A), was first considered to set up the conjugation strategy with mono- to tri- mannose saccharides. Through a detailed characterization of synthetized glycoconjugates, purification protocols and conjugation conditions have been revised and optimized with a dual aim: ensure high sugar-loading and avoid the presence of side reaction products. An alternative purification approach based on hydrophilic interaction liquid chromatography (HILIC) allowed the formation of glutaric acid conjugates to be avoided, and a design of experiment (DoE) approach led to optimal glycan loading. Once its suitability was proven, the developed conjugation strategy was applied to the chemical glycosylation of two recombinant antigens, native Ag85B and its variant Ag85B-dm, that are candidate carriers for the development of a novel antitubercular vaccine. Pure glycoconjugates (≥99.5%) were obtained. Altogether, the results suggest that, with an adequate protocol, conjugation via disuccinimidyl linkers can be a valuable approach to produce high sugar-loaded and well-defined glycovaccines
Affinity Capillary Electrophoresis as a Tool To Characterize Molecularly Imprinted Nanogels in Solution
No full textIn this work, an innovative and accurate affinity capillary electrophoresis (ACE) method was set up to monitor the complexation of aqueous MIP nanogels (NGs) with model cancer-related antigens. Using α2,6'- and α2,3'-sialyllactose as oversimplified cancer biomarker-mimicking templates, NGs were synthesized and characterized in terms of size, polydispersity, and overall charge. A stability study was also carried out in order to select the best storage conditions and to ensure product quality. After optimization of capillary electrophoresis conditions, injection of MIP NGs resulted in a single, sharp, and efficient peak. The mobility shift approach was applied to quantitatively estimate binding affinity, in this case resulting in an association constant of K around 106 M-1. The optimized polymers further displayed a pronounced discrimination between the two sialylated sugars. The newly developed ACE protocol has the potential to become a very effective method for nonconstrained affinity screening of NG in solution, especially during the NG development phase and/or for a final accurate quantitation of the observed binding
VERDANDI is a direct target of the MADS domain ovule identity complex and affects embryo sac differentiation in Arabidopsis
No full textIn Arabidopsis thaliana, the three MADS box genes SEEDSTICK (STK), SHATTERPROOF1 (SHP1), and SHP2 redundantly regulate ovule development. Protein interaction studies have shown that a multimeric complex composed of the ovule identity proteins together with the SEPALLATA MADS domain proteins is necessary to determine ovule identity. Despite the extensive knowledge that has become available about these MADS domain transcription factors, little is known regarding the genes that they regulate. Here, we show that STK, SHP1, and SHP2 redundantly regulate VERDANDI (VDD), a putative transcription factor that belongs to the plant-specific B3 superfamily. The vdd mutant shows defects during the fertilization process resulting in semisterility. Analysis of the vdd mutant female gametophytes indicates that antipodal and synergid cell identity and/or differentiation are affected. Our results provide insights into the pathways regulated by the ovule identity factors and the role of the downstream target gene VDD in female gametophyte development
Controlled Decoration of [60]Fullerene with Polymannan Analogues and Amino Acid Derivatives through Malondiamide-Based Linkers
No full textIn the last few years, nanomaterials based on fullerene have begun to be considered promising tools in the development of efficient adjuvant/delivery systems for vaccination, thanks to their several advantages such as biocompatibility, size, and easy preparation and modification. In this work we reported the chemoenzymatic synthesis of natural polymannan analogues (di- and tri-mannan oligosaccharides characterized by α1,6man and/or α1,2man motifs) endowed with an anomeric propargyl group. These sugar derivatives were submitted to 1,3 Huisgen dipolar cycloaddition with a malondiamide-based chain equipped with two azido terminal groups. The obtained sugar-modified malondiamide derivatives were used to functionalize the surface of Buckminster fullerene (C(60)) in a highly controlled fashion, and yields (11–41%) higher than those so far reported by employing analogue linkers. The same strategy has been exploited to obtain C(60) endowed with natural and unnatural amino acid derivatives. Finally, the first double functionalization of fullerene with both sugar- and amino acid-modified malondiamide chains was successfully performed, paving the way to the possible derivatization of fullerenes with immunogenic sugars and more complex antigenic peptides
The Case for Religious Moderation
No full textWe are, people will tell us, in the midst of a religious war. Depending on who you believe, either science is making us immoral heathens or religion is making us ignorant rubes. William Egginton, however, challenges this view. He not only claims that this dichotomy is false, he asserts that the two sides are both fundamentalists and cut from the same cloth. Egginton argues that we should all be religious moderates combining scientific truth with religious belief.
William Egginton is Andrew W. Mellon Professor in the Humanities and chair of the Department of German and Romance Languages and Literatures at the Johns Hopkins University. He is the author of In Defense of Religious Moderation, How the World Became a Stage, Perversity and Ethics, A Wrinkle in History, The Philosopher’s Desire, and The Theater of Truth. He is also coeditor of Thinking with Borges and The Pragmatic Turn in Philosophy, and translator of Lisa Block de Behar’s Borges: The Passion of an Endless Quotation.https://commons.und.edu/why-radio-archive/1085/thumbnail.jp
Northern Light, April 26, 1999
No full textThe staff (3)
Place to nurture futures is a necessity By: Echo Gamel (4)
Letters to the editor (4)
Pulitzer Prize-winning author speaks at UAA By: Gonzalo Medina 96)
Corrections (6)
Perspectives By: Jeff DeMartino (7)
Awards ceremonies perk up spirits at UAA By: Echo Gamel (8)
News Briefs (9)
Justice career fair brings the law to the Campus Center By: Echo Gamel (10)
Campus Scanner (11)
Students take to the streets to rally for university funding By: Echo Gamel (12)
Professor says goodbye to UA after 26 years of teaching By: Kathleen Tessaro (12)
Devilish Fun By: James Lobb (14)
KRUA Top 10 (14)
So Now What? By: Unknown (16-17)
Planning, relaxation key to stress-free finals By: Fran Victor (18)
Come one, come all By: Stephanie Tripp (20)
Art exhibition showcases gamut of themes By: Michael Conti (21)
Campus Calendar (22)
Box Seat (22)
Look, Ma By: Unknown (23)
Classified (24)
Know the facts about hepatitis C By: Daryl young (25)
P.S. Mueller By: Mueller (26)
Single Slices By: Peter Kohlsaat (26)
Rubes By: Leigh Rubin (26)
Comics (26)
Gutless comedy buried by slow-moving plot By: Erick Hayden (27)
‘Picasso’ offers robust blend of love, art, science By: Erick Hayden (27)
The thrill is in being there By: Patrick J. Paul (28)
Intramural Challenge more than just competition By: Patrick J. Paul (28)
Banquet honors women’s basketball By: Patrick J. Paul (28)
Ski team ends up in 6th place By: Justin Theriot (29)
NCAA lawsuit abuses race card By: Brian Winter (30
Northern Light, volume 3, number 6
No full textEarly registration for next semester to be conducted by phone By: Harold Schneider (1)
High-speed chase of teens ends at UAA By: Lyn Louise LaBarge (1)
Part-time professors save UAA money at expense of students By: Glenn Boledovich (1)
Bookstore makes profit of 159,000 in 1989 By: Janis Roller (2)
Retirement checks put on holds By: Robert Darling (2)
UAA can learn a lot from Davis By: Unknown (4)
USUAA News (4)
Letters to the editor (5)
The Northern Lights Scanner (6)
Activist, author Davis bashes U.S. foreign policy By: Echo Breaux (7)
Comic hypnotist causes barking among audience By: Ann LeBar (7)
Nuestra Columna By: David Ugarte (8)
Turkeys live in fear of Joe Bob Briggs By: Joe Bob Briggs (8)
UAA student will race hot-air balloon above India By: Mike Donovan Hausler (9)
Cysisyrata By: Beth Morris (10-11)
Dorms separate spouse BY: Jennifer Sunshine Miller (12)
Crosman teaching roles BY: Crystal Crouch (12)
Deaf student uses two kinds of speech By: Jessica Keil (13)
Highlight (14)
Single Slices By: Peter Kohlsaat (15)
Bent Offerings By: Don Addis (15)
Rubes By: Leigh Rubin (15)
Classified (16)
Sports injuries can cripple a team’s season strategy By: Scott Gere (17)
Skijoring fun for man and Rover By: George Nagel (17)
Skaters tie one, lose one to defending champions By: Scott Gere (18)
Shotokan club brings home six medals from Colorado By: Ashley Gouthro (19)
Lucky T-shirts, superstitions, help to win By: Scott “The Hammer” Samples (20)
Loud fans help hot spikers sweep By: Kerry Downing (20
Northern Light, volume 3, number 7
No full textBlack Caucus leader says UAA discriminates By: Echo Breaux (1)
Hirings upset black group By: Echo Breaux (1)
New regent chosen By: Richard Burt (1)
Mayoral candidates debate transportation and cultural center By: Glenn Boledovich (2)
Letters to the editor (3)
Don’t call on us, we’ll call you By: Unknown (4)
USUAA News (4)
Oh so dirty laundry By: Unknown (4)
UAA’s vehicles number 116 By: Robert Darling (5)
The Northern Light Scanner (5)
Safety taught at sexual assault seminars By; Crystal Crouch (7)
Middle East teach-in draws criticism of U.S. foreign policy By: David Ugarte (7)
Student from Switzerland studies nursing and language By: Scott Axtell (8)
Nuestra Columna By: David Ugarte (8)
Back to school at 29 isn’t easy By: Jessica Keil (10)
Pot: legal or not? By: Soren Wuerth (11)
Pot is beneficial, says holistic doctor By: Scott Axtell (12)
MD says pot poses health risk By: Harold Schneider (12)
Students respond to pot poll By: Glenn Boledovich (12)
Author hails hemp for global healing By: Glenn Boledovich (13)
‘Stigmata’ affects lead role in one-act play By: Shawnwne Albright (15)
Police discuss pot By: Janis Roller (16)
Jackstadt promotes economics By: Beth Morris (16)
Briggs offers exercise and movie advice By: Joe Bob Briggs (17)
Highlight (18)
Single Slices By: Peter Kohlsaat (19)
Bent Offerings By: Don Addis (19)
Rubes By: Leigh Rubin (19)
Classified (20)
UAA”s young pups tear up the ex-Seawolf hockey squad By: Scott Gere (21)
Seawolf volleyball team wins four team tourney By: Kerry Downing (22)
Two points are more pain than pleasure By: Scott “The Hammer” Samples (24)
Hanging out at next door’s climbing wall By: George Nagel (24
Northern Light, volume 17, number 9
No full textTechnology consultants get $40,000 By: Melissa Eichholz (1)
AWS reports offers details of accident By: Melissa Eichholz (1)
Grades on the phone result in less paperwork for students By: William K. Wolfrum (1)
University police investigate book trading service and owner By: Melissa Eichholz (1)
Administrators are fiddling By: Unknown (2)
Letters to the editor (2)
Comic By: Non Sequitur (2)
Rebelling against American perception of manhood By: Kokayi Nosakhere (3)
Former war hero speaks to students about gangs By: Jenny Sanders (4)
Early retirement program to entice UAA teachers By: Melissa Eichholz (6)
NSS job draws 60 applicants By: Jenny Sanders (6)
Gay Internet site offers free accounts to university students By: Northern Light Staff (6)
Campus Scanner (7)
Regents ready for Fairbanks By: Northern Light Staff (8)
UAA investigates violation By: Kokayi Nosakhere (9)
Senior Seminar examines UAA By: Northern Light Staff (9)
Pilot-turned-author to read from first novel By: Rose Cox (11)
National Geographic photographer brings whales to lecture series By: Tataboline Brant (11)
Gallery strives to capture depth of Alaska wildlife By: Fryderyk Frontier (12)
Artists display to help hunger; not for prizes By: Fryderyk Frontier (13)
Tundra By: Chad Carpenter (14)
Single Slices By: Peter Kohlsaat (14)
Larger Than Life By: David Gallagher (14)
Off the mark By: Mark Parisi (14)
P.S. Mueller By: Mueller (14)
Rubes By: Leigh Rubin (14)
Dave By: David Miller (14)
Astrological Guru (15)
Classified (15)
Top Ten the EDGE KRUA 88.1 FM (16)
Campus Calendar (16)
Calendar Highlight (16)
Avoid the seasonal bug: flu shot schedule (16)
Unlikeable main character spoils “The Game” By: Michael Marcella (16)
Student Activities works hard to hypnotize students By: Echo Gamel (17)
First Amendment protection heart of Banned Book Week By: Fryderyk Frontier (17)
Supersuckers rock Anchorage crowd By: Jason Rand (18)
Sawaddi Thai brings authentic food and amazing experiences By: William M. Stenzel (19)
Science and Lecture Series works to appeal to the general public By: Echo Gamel (19)
Ways to a healthier cervix By: Nancy E. Brew (20)
Sports Shorts (21)
UAA runners give helping hand in Tom Besh fund raiser By: James Wagner (22)
The Hajdukovichs’ Sibling Rivalry By: Chris Curtis (23)
Seawolves sweep SPC By: Chris Curtis (24)
Defense carries UAA through season opener By: Chris Curtis (24)
Effort not limited to gender By: Chris Curtis (24
