2,513 research outputs found
Benjamin Britten, Piper and Aldeburgh
No full textPrinted items designed by John Piper for covers of printed music by Benjamin Britten and for the Aldeburgh Festiva
Introduction: John Piper in context
No full textA discussion of the printed work of the artist John Piper in relation to his influences, contemporaries and use of techniques and his own writings on the subject
AKTIVITAS INFUSA DAUN Piper betle Linn DAN Piper crocatum Ruiz & Pav TERHADAP VIABILITAS SEL HeLa Activity of the infusion of Piper betle L. and Piper crocatum Ruiz & Pav on HeLa cell line viability
No full textABSTRACTPiper betle L. (betel leaves) and red betel (Piper crocatum Ruiz & Pav) are medicinal plant that are often usedby the Indonesian people to treat various diseases, including cancer. Scientific evidence to strengthen then useas anticancer is still necessary. This study was conducted to determine the effect of green and red betel leafinfusion against HeLa cervical cancer cell viability, as well as its potential to inhibit the expression of Bcl2 protein qualitatively. Infusionof betel leaves were dried over a water bath to obtain a dry extract. Effect of extracts on theviability of HeLa cells determined by the cytotoxic test with MTT assay method to obtain IC50 values . Effect theactivity against Bcl2 protein was tested byimmunocyitochemical test. Cytotoxic test infution of green betel leavesgave IC50 value of 49.14 ug/ml, whereas the red betel showed no cytotoxic effects. The immunocytochemical testshowed a reduction in Bcl-2 protein expression.The results of this study showed that green betel leaves affect HeLacell viability by inducing cell death through inhibition of anti-apoptotic Bcl2 protein expression. Thus green betelleaves are potential to be developed as a chemopreventive agent.Keywords: Piper betle, Piper crocatum, cytotoxic, HeLa cells ABSTRAKSirih hijau(Piper betle L.) dan sirih merah (Piper crocatum Ruiz & Pav) merupakan tanaman yang seringdimanfaatkan oleh masyarakat Indonesia untuk mengobati berbagai macam penyakit, termasuk kanker. Buktiilmiah untuk memperkuat dasar penggunaannya sebagai antikanker masih sangat diperlukan. Penelitian inidilakukan untuk mengetahui efek infusa daun sirih hijau dan sirih merah terhadap viabilitas sel kanker serviksHeLa, serta potensinya dalam menghambat ekspresi protein antiapoptosis Bcl2 secara immunositokimia.Infusa sirih dikeringkan di atas penangas air hingga diperoleh ekstrak kering. Pengaruh ekstrak terhadapviabilitas sel HeLa ditetapkan melaluiuji sitotoksik dengan metode MTT assay hingga diperoleh nilai IC50.Pengaruh ekstrak aktif terhadap protein Bcl2 dilakukan dengan pengecatan immunositokimia. Uji sitotoksikinfusa sirih hijau memberikan nilai IC50 49.14 μg/ml, sedangkan sirih merah tidak menunjukkan efek sitotoksik.Hasil pengecatan immunositokima secara kualitatif memperlihatkan pengurangan ekspresi protein Bcl2.Hasil penelitian ini menunjukkan bahwa sirih hijau mempengaruhi viabilitas sel HeLa dengan menginduksikematian sel melalui penghambatan ekspresi protein antiapoptosis Bcl2. Dengan demikian sirih hijau cukuppotensial dikembangkan sebagai agen kemopreventif untuk kanker serviks
Effect of Piper betle and Brucea javanica on the Differential Expression of Hyphal Wall Protein (HWP1) in Non-Candida albicans Candida (NCAC) Species
No full textThe study aimed to identify the HWP1 gene in non-Candida albicans Candida species and the differential expression of HWP1 following treatment with Piper betle and Brucea javanica aqueous extracts. All candidal suspensions were standardized to 1 x 10(6) cells/mL. The suspension was incubated overnight at 37 degrees C (C. parapsilosis, 35 degrees C). Candidal cells were treated with each respective extract at 1, 3, and 6 mg/mL for 24 h. The total RNA was extracted and reverse transcription-polymerase chain reaction was carried out with a specific primer of HWP1. HWP1 mRNAs were only detected in C. albicans, C. parapsilosis, and C. tropicalis. Exposing the cells to the aqueous extracts has affected the expression of HWP1 transcripts. C. albicans, C. parapsilosis, and C. tropicalis have demonstrated different intensity of mRNA. Compared to P. betle, B. javanica demonstrated a higher suppression on the transcript levels of HWP1 in all samples. HWP1 was not detected in C. albicans following the treatment of B. javanica at 1 mg/mL. In contrast, C. parapsilosis and C. tropicalis were shown to have HWP1 regulation. However, the expression levels were reduced upon the addition of higher concentration of B. javanica extract. P. betle and B. javanica have potential to be developed as oral health product.Article Link :
http://www.hindawi.com/journals/ecam/2013/397268
Estudo fitoquímico e biológico da Piper solmsianum C.DC. variedade solmsianum (Piperaceae)
No full textTese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências Físicas e Matemáticas. Programa de Pós-Graduação em Química.O presente trabalho envolveu processos de extração, isolamento, purificação e identificação dos constituintes químicos das folhas de outono da Piper solmsianum. Da fração hexânica foram isoladas três neolignanas benzofurânicas denominadas de euponatenóide-3, eupomatenóide-5 e conocarpano. Da fração diclorometano foi isolado o conocarpano. Na fração acetato de etila foram detectados as flavonas 7- metoxi-apigenina (genkwanina) e a orientina e duas flavanonas hesperidina e iluteolina-7-rutinosídeo. O conocarpano foi submetido a reações de metilação, benzoilação e acetilação e obteve-se os derivados: conocarpano metilado, conocarpano benzoilado e conocarpano acetilado, os quais foram avaliados quanto ao potencial analgésico e observou-se que o conocarpano cetilado foi mais ativo que os demais. Por reações de dimerização oxidativa do isoeugenol obteve-se dois análogos ao conocarpanp o a-di-isoeugenol e o diidrodiisoeugenol, os quais demonstra
Cytotoxic Amides from Piper Sintenense
No full textA new alkaloid, pipersintenamide (1), together with fourteen known compounds, have been isolated from the whole plant of Piper sintenense. The structures of these compounds were elucidated by spectroscopic analysis. Pipersintenamide, sinten-pyridone, sarmentine, and 1-(3,4- methylenedioxyphenyl )-1E-dodecene at 20mug/ml exhibited effective cytotoxicities (cell survival < 15%) against CCRF-CEM, HL-60, PC-3, and HA 22T cell lines
Merry Piper
No full textA traveling piper enjoys his life.https://egrove.olemiss.edu/kgbsides_uk/1830/thumbnail.jp
Chemical constituents, antioxidant, and cytotoxicity of essential oils of Piper arborescens and Piper caninum
No full textEssential oils of the stem bark of Sarawak's wild pepper species namely the Piper arborescens and Piper caninum were extracted by using Clevenger's water distillation method, and analysis using gas chromatography-flame ionization detector (GC-FID) and gas chromatography-mass spectroscopy (GC-MS) have identified a total of 54 and 57 chemical components in the essential oils, respectively. Three major compounds have been identified in the essential oil of Piper arborescens namely the pentadecanal (18.88%), guaiol (11.19%), and β-guaiene (11.12%). In the essential oil of Piper caninum, four main compounds identified were isocaryophyllene (20.60%), (E)-α-bergamotene (13.74%), (E)-isoeugenol (13.46%), and (E,Z)-3,6-nonadien-1-ol (9.35%). Evaluation of antioxidant properties showed the EC50 values of essential oils of Piper arborescens and Piper caninum were 249.30 and 238.70 µg/mL, respectively, indicating low scavenging activity against DPPH as compared to ascorbic acid as standard with EC50 value of 2.72 µg/mL. Cytotoxicity assay showed that average death of Artemia salina brine shrimp in the essential oil of Piper arborescens was higher, with LC50 57.95 µg/mL, as compared to 249.74 µg/mL of essential oil of Piper caninum. The cytotoxic level does not always indicate its outright toxicity but may also indicate the presence of potential natural cytotoxic components, especially in essential oil of Piper arborescens as suggested by Elumba et al. (2013)
Atividade tripanocida de Piper arboreum e Piper tuberculatum (Piperaceae)
No full textIn the scope of our ongoing research on bioactive agents from Brazilian flora, twenty-four extracts and fractions obtained from Piper arboreum Aub. and Piper tuberculatum Jacq. (Piperaceae) were screened for trypanocidal activity by using MTT colorimetric assay. The strongest activity was found in hexane fractions from the leaves of P. arboreum (IC50= 13.3 µg/ mL) and P. tuberculatum (IC50 = 17.2 µg/mL). Hexane fractions of the fruits of P. tuberculatum and P. arboreum showed potent toxic effects on epimastigote forms of Trypanosoma cruzi, with values of IC50 (µg/mL) of 32.2 and 31.3, respectively. Additionally, the phytochemical study of the hexane fraction of P. arboreum leaves furnished two pyrrolidine amides, piperyline (1) and 4,5-dihydropiperyline (2), which could be responsible, at least in part for the observed antiprotozoal activity.No escopo de nossas pesquisas sobre agentes bioativos da flora brasileira, vinte e quatro extratos e frações de Piper arboreum Aub. e Piper tuberculatum Jacq. (Piperaceae) tiveram sua atividade tripanocida avaliada através do ensaio colorimétrico com MTT. As atividades mais potentes foram manifestadas pelas frações hexânicas das folhas de P. arboreum (CI50 = 13,3 µg/mL) e P. tuberculatum (CI50 = 17,2 µg/mL). As frações hexânicas dos frutos de P. tuberculatum e P. arboreum também apresentaram efeito tóxico potente contra as formas epimastigotas de Trypanosoma cruzi, com valores de CI50 (µg/mL) de 32,2 e 31,3, respectivamente. Adicionalmente, o estudo fitoquímico da fração hexânica das folhas de P. arboreum forneceu duas amidas pirrolidínicas, piperilina (1) e 4,5-diidropiperilina (2), que podem ser responsáveis pela atividade antiprotozoária desta fração.Fundação de Amparo à Pesquisa do Estado de São Paulo - Programa BIOTA-FAPESP (The Biodiversity Virtual Institute
Prospeccção fitoquímica e atividade antioxidante do extrato bruto e frações de Piper marginatum
No full textThe Piperaceae family belongs to the order Piperales and has five genera, namely: Verhuellia, Zippelia, Manekia, Piper, and Peperomia. The genus Piper has approximately 1000 species, and many of them have pharmacological properties. The species Piper marginatum is known for its use as an analgesic, anti-inflammatory, and hemostatic in popular medicine and for having many biological activities evidenced, such as fungicide, larvicide, and cercaricide besides the antioxidant activity of its essential oils. Free radicals are molecules or part of molecules that have one or more unpaired electrons and are the cause of many diseases such as cancer, Alzheimer's, and Parkinson's, besides the natural aging process that affect all kinds of people indiscriminately, increasing the number of researches aiming to find ways to prevent these diseases. Antioxidants are molecules capable of stabilizing free radicals, by donating electrons, preventing the propagation of the reactions that originate them. The present work aimed to characterize the chemical profile and to evaluate the antioxidant activity of the crude extract and fractions of Piper marginatum through tube chromatic assay, thin-layer chromatography, and DPPH radical inhibition. The phytochemical prospection of the crude extract and fractions of Piper marginatum demonstrated the presence of substances such as phenolic compounds, flavones, flavonols, flavanones, terpenes, xanthones, and catechins. The test carried out in TLC to evaluate the antioxidant activity showed positive results for the crude extract and fractions of Piper marginatum, the quantitative antioxidant test showed better findings for fractions in ethyl acetate, n-butyl alcohol, and hydroalcoholic with IC50 values of 29. 97, 32.93, and 42.70 µg/mL accordingly, this activity may be associated with substances identified in the phytochemical prospection tests. These results can serve as a basis for the research and development of products with diverse application areas.A família Piperaceae pertence à ordem Piperales e possui cinco gêneros, a saber: Verhuellia, Zippelia, Manekia, Piper e Peperomia. O gênero Piper possui aproximadamente 1000 espécies e muitas delas apresentam propriedades farmacológicas. A espécie Piper marginatum é conhecida por seu uso como analgésico, antiinflamatório e hemostático na medicina popular e por possuir muitas atividades biológicas evidenciadas, tais como: fungicida, larvicida e cercaricida além da atividade antioxidante de seus óleos essenciais. Os radicais livres são moléculas ou parte de moléculas que possuem um ou mais elétrons desemparelhados e são causadores de muitas doenças como o câncer, Mal de Alzheimer e Parkinson, além do processo natural de envelhecimento que afetam indiscriminadamente todos os tipos de pessoas, aumentando o número de pesquisas com a finalidade de buscar formas de prevenção dessas doenças. Os antioxidantes são capazes de estabilizar os radicais livres, pela doação de elétrons, impedindo a propagação das reações que os originam. O presente trabalho objetivou caracterizar o perfil químico e avaliar a atividade antioxidante do extrato bruto e frações de Piper marginatum através de reações cromáticas em tubos, cromatografia em camada delgada e inibição do radical DPPH. A prospecção fitoquímica do extrato bruto e frações de Piper marginatum demonstrou a presença de substâncias como compostos fenólicos, flavonas, flavonois, flavanonas, terpenos, xantonas e catequinas. O ensaio realizado em CCD para avaliar a atividade antioxidante apresentou resultados positivos para o extrato bruto e frações de Piper marginatum, já o ensaio antioxidante quantitativo demonstrou melhores resultados para as frações em acetato de etila, butanol e hidrooalcoolica, com valores de IC50 de 29,97, 32,93 e 42,70 µg/mL respectivamente, esta atividade pode estar associadas as substâncias identificadas nos ensaios da prospecção fitoquímica. Estes resultados podem servir como base para pesquisa e desenvolvimento de produtos com diversas áreas de aplicação.3Si
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