7,091 research outputs found
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Epitope characterization of a monoclonal antibody that selectively recognizes KIR2DL1 allotypes
No full textKiller immunoglobulin-like receptor (KIR) genes code for a family of inhibitory and activating receptors, finely tuning NK cell function. Numerous studies reported the relevance of KIR allelic polymorphism on KIR expression, ligand affinity, and strength in signal transduction. Although KIR variability, including gene copy number and allelic polymorphism, in combination with HLA class I polymorphism, impacts both KIR expression and NK cell education, only a precise phenotypic analysis can define the size of the different KIRpos NK cell subsets. In this context, reagents recognizing a limited number of KIRs is essential. In this study, we have characterized the specificity of an anti-KIR mAb termed HP-DM1. Testing its binding to HEK-293T cells transfected with plasmids coding for different KIRs, we demonstrated that HP-DM1 mAb exclusively reacts with KIR2DL1. Using site-directed mutagenesis, we identified the four amino acids relevant for HP-DM1 recognition: M44, S67, R68, and T70. HP-DM1 mAb binds to a conformational epitope including M44, the residue crucial for HLA-C K80 recognition by KIR2DL1. Based on the HP-DM1 epitope characterization, we could extend its reactivity to all KIR2DL1 allotypes identified except for KIR2DL1*022 and, most likely, KIR2DL1*020, predicting that it does not recognize any other KIR with the only exception of KIR2DS1*013. Moreover, by identifying the residues relevant for HP-DM1 binding, continuously updating of its reactivity will be facilitated.This work was supported by Italian Ministry of Health (Ricerca Corrente G. Gaslini, Ricerca Corrente Ospedale Policlinico San Martino) (Cristina Bottino and Daniela Pende), Instituto de Salud Carlos III, FIS00/0181 (to Miguel Lopez-Botet), H2020-MSCA-ITN-2017-765104-MATURE-NK (to Miguel Lopez-Botet and Daniela Pende), AIRC 5×1000 2018 id. 21147 (Lorenzo Moretta)
Imparare le lingue per abbracciare il mondo
No full textL’autrice che si colloca, come Daniela Zorzi, tra i fautori di una linguistica “impegnata”, propone in ricordo della studiosa amica, una riflessione sul ruolo cruciale del multilinguismo e delle lingue- culture, per il successo professionale e il benessere sociale, nella società contemporanea. Il multilinguismo appare come un’urgenza educativa che interpella studiosi e formatori a tutti i livelli al fine di promuovere nei giovani un apprendimento consapevole efficace ed autonomo.Multilingualism constitutes an urgent objective for education, which needs to involve teachers and researchers at all levels if we are to promote effective and autonomous learning in the young. Like Daniela Zorzi, the author considers herself a socially engaged linguist, and in memory of her friend’s work, offers a reflection on the key roles of languages and cultures and of multilingualism for professional success and social welfare today
Reclamações ambientais em Aveiro, Portugal: atores, preocupações, padrão territorial e resoluções
No full textThis paper presents an environmental diagnosis based on public complaints on environmental
issues submitted to the Environmental Department of the Aveiro City Council, Portugal, between
2000 and 2005. It discusses the potential influences of these in local environmental planning
and governance. The paper has been organised into five sections. The first of these introduces
the study. The second section focuses on the conceptual approaches relating to environmental
grassroots movements, the main actors involved in these movements and the role played by
local government. It also contains a brief review of the most recent urban environmental
quality challenges in the European context together with a description of the main features of
the associated political and legal framework in Portugal. The third section describes the case
study and the methodology used. The results of the empirical study are detailed in the fourth
section. The final section critically analyses these results with emphases on the temporal
evolution of the submission of complaints, the actors involved, the local environmental problems
and their associated spatial pattern as well as the responses given by the City Council. This
information may then be used to provide a useful indicator for the perception of environmental
quality as well as a credible instrument for the visualisation and evaluation of local performance
in terms of environmental planning and management.AlBan Programme - n. E05M053040B
La poesía de improvisación en la“Gara poética” sarda y en la “Controversia” cubana. Diferencias y similitudes.
No full textJak rozumieć „historyczność” biblijnego Daniela? Różne kierunki wyjaśniania orędzia Księgi Daniela
No full textThe subject of this article is the issue of the historicity of the figure of Daniel, who is the main character of the biblical book bearing his name. The question of whether Daniel is a historical or just a literary figure is important because it influences the exegesis of the text of the Book of Daniel. First, the author will indicate the reasons for questioning the historicity of Daniel, and then he will present different approaches to interpreting the content of the Book of Daniel.Przedmiotem artykułu jest kwestia historyczności postaci Daniela, który jest głównym bohaterem księgi biblijnej noszącej jego imię. Pytanie o to, czy Daniel jest postacią historyczną, czy tylko literacką, jest ważne z tego powodu, że ma wpływ na egzegezę tekstu Księgi Daniela. Najpierw autor wskaże powody, na podstawie których podważa się historyczność Daniela, a następnie przedstawi różne podejścia w interpretacji treści Księgi Daniela
Los servicios intensivos en conocimiento: Una oportunidad para diversificar la estructura exportadora de la Argentina
No full textEste trabajo intenta explorar en qué medida hay una oportunidad para diversificar la estructura productiva y exportadora de la Argentina en el sector servicios, y, si dicha oportunidad existe, cuáles son las condiciones requeridas para aprovecharla. Nos concentramos en un segmento de servicios (a los cuales denominamos intensivos en conocimiento) que se caracterizan por ser intensivos en trabajo calificado y por emplear tecnologías de avanzada para su prestación, incluyendo fundamentalmente las TICs, las cuales habilitan además su provisión a larga distancia.Fil: Lopez, Andres Flavio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martin. Escuela de Economia y Negocios. Centro de Investigaciones Para la Transformacion.; ArgentinaFil: Ramos, Daniela. Universidad Nacional de San Martin. Escuela de Economia y Negocios. Centro de Investigaciones Para la Transformacion.; Argentin
Servicios basados en conocimiento y desarrollo en América Latina
No full textEl buen desempeño macroeconómico de América Latina entre 2004 y 2011 (solo interrumpido en 2009 por los efectos de la crisis sistémica global) generó expectativas sobre el inicio de un posible sendero de crecimiento sostenido. Sin embargo, debido a una combinación de circunstancias externas e internas, en años recientes la situación se deterioró severamente (especialmente en Sudamérica), alejando de nuevo la esperanza de salvar las trampas del ingreso medio o bajo en el que se encuentran sumidos los países de la región.Fil: Lopez, Andres Flavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Saavedra 15. Instituto Interdisciplinario de Economía Politica de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Económicas. Instituto Interdisciplinario de Economía Politica de Buenos Aires; ArgentinaFil: Ramos, Daniela. Centro de Invest.para la Transformacion; Argentin
Los servicios intensivos en conocimiento: Una oportunidad para diversificar la estructura exportadora de la Argentina
No full textEste trabajo intenta explorar en qué medida hay una oportunidad para diversificar la estructura productiva y exportadora de la Argentina en el sector servicios, y, si dicha oportunidad existe, cuáles son las condiciones requeridas para aprovecharla. Nos concentramos en un segmento de servicios (a los cuales denominamos intensivos en conocimiento) que se caracterizan por ser intensivos en trabajo calificado y por emplear tecnologías de avanzada para su prestación, incluyendo fundamentalmente las TICs, las cuales habilitan además su provisión a larga distancia.Fil: Lopez, Andres Flavio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martin. Escuela de Economia y Negocios. Centro de Investigaciones Para la Transformacion.; ArgentinaFil: Ramos, Daniela. Universidad Nacional de San Martin. Escuela de Economia y Negocios. Centro de Investigaciones Para la Transformacion.; Argentin
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