Datasets that support the figures published in Lemos et al., Cells (2023). The project aims to elucidate the role of the metabolic enzyme PKM2 in intracellular Ca2+ signaling. We demonstrated that PKM2 can differentially regulate cytosolic and mitochondrial Ca2+ handling. In the cytosol, PKM2 interacts with and inhibits IP3R activity, while in the mitochondria, PKM2 modulates the Ca2+ signal by influencing mitochondrial metabolism, structure, and dynamics. By using the TAT-D5SD peptide, corresponding to amino acids 2078-2098 of IP3R1, which disrupts the PKM2:IP3R complex, we observed that the effects of this peptide on Ca2+ handling and apoptosis depend on the IP3R but not on PKM2. Furthermore, the presence of PKM1 and the tyrosine kinase Lck is not required, suggesting the presence of one or more other proteins that can interact with the IP3R and form a complex at the same site as PKM2 on the IP3R
This dataset includes all the data integrated in the publication in Lemos et al., 2024, BBA-MCR and contains all data necessary to replicate the published figures. The project investigates the nature and the properties of the metabolic enzyme PKM2 in the regulation of IP3R-mediated Ca2+ signaling. Using both chemical and genetic methods, we provide evidence that the catalytical active, tetrameric form of PKM2 preferentially suppresses IP3R-mediated Ca2+ release. Moreover, our data strongly suggests that this inhibitory effect necessitates an additional cellular factor, which is most likely GRP75.
All info concerning the integrated data can be found in the attached "README-BBA final" file
In Brazil, for many years, the production of marine shrimp was associated with estuarine, apicum, and
mangrove regions. However, the last few years have been marked by a pronounced expansion to inland
regions, an activity known as marine carciniculture. In the state of Alagoas, Northeast of Brazil, the
harsh climate region has the newest Brazilian centers of Litopenaeus vannamei shrimp production in
continental waters: Arapiraca, Craíbas, Coité do Nóia, Feira Grande, Girau do Ponciano, Igaci, Lagoa
da Canoa, Limoeiro de Anadia, Olho D'Água Grande and Taquarana. For these municipalities, the
activity emerged as an alternative for generation of employment and income for farmers who suffered
and still suffer with the decline of agricultural production due to the disuse of land, by the salinization
of water from rivers, creeks, and artesian wells. As it is relatively recent in the region, there are few
records about its implementation. In view of this, the objective was to characterize the carciniculture in
the harsh climate region of Alagoas by the investigation of its participants and its ways of production.
To accomplish this task, 40 family farmers were interviewed by using a semi-structured questionnaire.
In addition, free observations were made and informal accounts were collected during the years 2020 to
2022. From this, it was found that local carciniculture started a little over three years ago and only in
2018 gained commercial character having presented annual income of up to 300,000.00.TheactivityinthemunicipalitiesofAlagoashasbeendonewiththehelpoftechnicaladvice,andwiththeconstructionpatternofnurseriesfromotherproductioncenters,similartothesystemsimplementedinthestateofParaıˊba.Thefarmers,withthehelpofprofessionalsandknowledgeacquiredovertime,haveadaptedtothenewproductiveactivity,whencomparingthemethodologyimplementedinharshclimateregionofAlagoaswithotherregions,ispossibletoidentifypeculiarities,thetiesandbondscreatedbetweenthoseinvolvedandtheworkenvironment.Somecuriositiesalsodrawattention,suchasthevariationsinstockingdensitiesobservedindifferentpoles,somethingcommoninallpolesisthedecreaseinstockingdensityinrainyperiods,highrainfallthatresultedindecreasedwatertemperature,whichcancontributetotheproliferationofpathogensinthecultureenvironment.Theanalysisofthequestionnairesshowedthatmalefamilyfarmersrepresent82.5climateregion,whilewomencorrespondonlyto17.5hoursaday.Thesizeoftheevaluatedfarmsvariesbetween1to50hectares,andthepondsbuiltonthemarebetween1000m2and4000m2,withanaverageproductionof500kgto4500kgpermonthperfarm.Thepriceperkilogramofshrimpsoldvariedbetween 12.51 and R13.64,beinginfluencedbythetimeoftheyear,amongotherfactors.Itwasevidencedthatthepoleswithgreaterproductiverelevanceare:Arapiraca,CoiteˊdoNoˊia,IgaciandLimoeirodeAnadia.Amongtheinterviewees,few(17.5diversifytheagriculturalproductiontogetenoughincomeforsubsistence.Fromtheinterviews,itwaspossibletoidentifythatmorethan50thisisafairlynewactivity,itisstillnotknownforsurewhichimpactsorenvironmentaldamagemaybecausedinthelongtermbytheactivityinthisregion.Inthissense,mostofthefamilyfarmersaffirmthattheyhaveneverbeenvisitedbyenvironmentalinspectionagencies.Despitethis,allareawareoftheimportanceofenvironmentallicensingandclaimtobeasignificantstepforshrimpproduction.Regardingthedischargeofeffluents,most(55dischargeintowaterbodies.However,mostfamilyfarmers(80fromthepondsharmfultotheenvironment.Infact,technicalassistancewasoneofthekeyfactorsfortheconsolidationandsuccessoftheproductiveactivityintheregion.Itwaspossibletoobservefromthefarmers′reportshowstartingwithtechnicalassistancemadeallthedifference.Thedatacollectedfromtheintervieweeswerecomparedwiththedatacollectedfromthesecretaries,itwaspossibletonoticethattheenvironmentalandgovernmentalorganslinkedtothecarcinicultureactivitystilldon′thave,orintheirmajority,withlimitedinformation,whatcanhaverepercussions,directly,inanegativeway,inthedevelopmentofpublicpoliciesdirectedtomarineCarciniculture.Inaddition,itispossibletoverifytheexistenceofbarrierstothestrengtheningoftheactivity,beingtheissuesrelatedtoenvironmentallicensingthemostrelevant,amongthem:difficultiesinfindingqualifiedprofessionalstoperformtheservices,thehighfeescharged,theslownessoftheenvironmentalinspectionagencies.NoBrasil,pormuitosanos,aproduc\ca~odecamara~omarinhoesteveassociadaaregio~esestuarinas,deapicumemanguezais.Noentanto,osuˊltimosanosdezanos,foimarcadoporumaacentuadaexpansa~oparaasregio~esinterioranas,aatividadeconhecidacomocarciniculturacontinental.NoestadodeAlagoas,NordestedoBrasil,aregia~odoAgrestecontacomosmaisnovospoˊlosbrasileirosdeproduc\ca~odocamara~oLitopenaeusvannameiemaˊguascontinentaisquesa~o:Arapiraca,Craıˊbas,CoiteˊdoNoˊia,FeiraGrande,GiraudoPonciano,Igaci,LagoadaCanoa,LimoeirodeAnadia,OlhoD’AˊguaGrandeeTaquarana.Paraestesmunicıˊpios,aatividadesurgiucomoalternativaparagerac\ca~odeempregoerendaaosagricultoresquesofrerameaindasofremcomodeclıˊniodaproduc\ca~oagrıˊcoladevidoainutilizac\ca~odasterras,pelasanilizac\ca~oaolongodosanosdasaˊguasderios,riachosepoc\cosartesianos.Porserrelativamenterecentenaregia~o,poucossa~oosregistrosacercadesuaimplantac\ca~o.Diantedisso,teve−seporobjetivocaracterizaracarciniculturanoAgrestealagoanoapartirdainvestigac\ca~odeseusparticipanteseseusmodosdeproduc\ca~o.Paratal,foramentrevistados40carcinicultoresfamiliarespormeiodeumquestionaˊriosemiestruturado.Ademais,foramrealizadasobservac\co~eslivresecoletadososrelatosinformaisduranteosanosde2020a2022.Constatou−sequeacarciniculturalocalteveinıˊciohaˊpoucomaisdetre^sanos,esomenteem2018ganhoucaraˊtercomercialtendoapresentadorendimentoanualdeateˊR 300.000,00. A
atividade nos referidos municípios alagoanos tem sido feita com auxílio de consultoria técnica
e com padrão de construção de viveiros de outros pólos produtivos, similar aos sistemas
implatados no estado da Paraíba. Os produtores rurais, com o auxílio de profissionais e do
conhecimento adiquirido ao longo do tempo, adaptaram-se a nova atividade produtiva, quando
comparado à metodologia implantada no Agreste de Alagoas com outras regiões, é possível
identicar peculiaridades quanto as características produtivas peculiares dos envolvidos e o
ambiente de trabalho. Algumas aspectos da carcicultura do Agreste também chamam a atenção,
a exemplo das variações de densidades de estocagem observadas em diferentes polos. Algo
comum em todos os polos é a diminuição da densidade de estocagem em períodos chuvosos,
alta pluviosidade que acarretaram em diminuição da temperatura da água, o que pode contribuir
para a proliferação de patégeno do ambiente de cultivo. A análise dos questinários mostrou que
agricultor familiar homens representam 82,5% do total de produtres do Agreste, enquanto as
mulheres correspondem apenas a 17,5 %. Ambos os sexos trabalham na atividade, em média,
8h diárias. O tamanho das propriedades avaliadas varia entre 1 a 50 hectares, e os viveiros nelas
construídos possuem entre 1000 m2 e 4000 m2, apresentando produção média de 500 kg a 4500
kg mensais por propriedade. O custo médio por quilograma de camarão produzido variou entre
R12,51eR 13,64, sendo influenciado pela época do ano. Evidenciou-se que os pólos com maior relevância produtiva são: Arapiraca, Coité do Nóia, Igaci e Limoeiro de Anadia. Entre
os entrevistados poucos (17,5%) são os que têm a carcinicultura como sua principal fonte de
renda, em sua maioria, os carcinicultores familiares diversificam a produção agropecuária para
conseguirem renda suficiente para subsistência. A partir das entrevistas, foi possível identificar
que mais de 80% dos proprietários receberam assistência técnica no em 2021. Por se tratar de
uma atividade relativamente nova, ainda não se sabe ao certo quais impactos ou danos
ambientais podem ser causados a longo prazo pela atividade nesta região. Neste sentido, a
maioria dos carcicicultores (40%) afirma nunca terem sido visitados por órgãos fiscalizadores
ambientais. Apesar disso, todos os entrevistados estão cientes da importância do licenciamento
ambiental e afirmam ser esta uma etapa significativa para a produção de camarão. Com relação
ao descarte de efluentes, a maior parte (55%) dos carcinicultores realizam o tratamento da água
antes do descarte nos corpos hídrico. Contudo, a maior parte dos produtores (80%) não
consideram a água gerada a partir dos viveiros prejudicial ao meio ambiente. De fato, a
assistência técnica foi um dos fatores primordiais para a consolidação e sucesso da atividade
produtiva na região. Os dados coletado com os entrevistados foram comparados com os dados
coletados junto às secretarias muncipais de Agricultura, Meio Ambiente e Instituto de Meio
Ambiente do estado, e dessa forma foi possível perceber que os órgãos ambientais e
governamentais vinculados à atividade de carcinicultura ainda não possuem, em sua maioria,
informações limitadas o que pode repercutir,diretamente, de forma negativa, no
desenvolvimento de políticas públicas direcionadas à Carcinicultura marinha. Além disso, é
possível constatar a existência de barreiras quanto ao fortalecimento da atividade, sendo as
questões vinculadas ao licenciamento ambiental as de maior relevância, entre elas: dificuldades
em encontrar profissionais capacitados para realização dos serviços, os altos valores cobrados
e a morosidade dos órgãos licenciadores
The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
We conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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