12 research outputs found
Introduction of Neottia nidus-avis (L.) Rich. into culture in vitro
No full textCallus cultures from ovary and ovule of Neottia nidus-avis (L.) Rich. were obtained. Dependence of callus genesis on growth regulators composition of nutrient medium it was shown. Cytomorphological analysis of callus cultures demonstrat their high morphogenetic potential
Role of phytolectin in the life cycle of plants
No full textIn the review the basic properties of plant lectins and their physio- logical role in the life cycle of plants are considered. There are data on the current state of the researches of phytolectins and lectin-like pro- teins as well as designation of the main directions of further study on the functioning of these proteins in the plant physiological processes.N. V. Kovalchuk, N. M. Melnykova, L. I. Musatenk
Темпы роста изолятов Aspergillus niger L. из контрастных условий существования
No full textEighteen strains of filamentous fungus Aspergillus niger were isolated from contrasting environments in Israel: the hypersaline Dead Sea and the temperate «Evolution Canyon» I. A comparison of growth rates under different water activity stress was provided. Clear differences in growth rates were observed under 5—20 % and 40 % volumes of the Dead Sea water (DSW). No difference was observed when we used 25— 35 % volumes of DSW. Strains from «Evolution Canyon» I grown at lower concentrations of the DSW revealed higher growth rates, while strains from the DSW demonstrated higher activity under higher stress (40 % volumes of DSW). Therefore, the following conclusion can be made: strains of A. niger isolated from the DSW are more adapted to stress associated with low-water activity.Сравнение темпов роста спор изолятов Aspergillus niger L. из контрастных по солености условий существования: гиперсоленых — Мертвого моря и умеренных — Европейского и Африканского склонов "Эволюционного каньона" (Израиль) дало возможность сделать вывод о большой адаптированности спор изолятов A. niger, выделенных из воды Мертвого моря, к стрессу, ассоциированному с низкой активностью воды.Порівняння темпів росту ізолятів Aspergillus niger з контрастних умов існування: гіпер-солоних — Мертвого моря і помірних — "Еволюційного каньйону" (Ізраїль) дало можливість дійти висновку про більшу адаптованість ізолятів A. niger, виділених з води Мертвого моря, до стресу, асоційованого з низькою активністю води
Темпы роста изолятов Aspergillus niger L. из контрастных условий существования
No full textEighteen strains of filamentous fungus Aspergillus niger were isolated from contrasting environments in Israel: the hypersaline Dead Sea and the temperate «Evolution Canyon» I. A comparison of growth rates under different water activity stress was provided. Clear differences in growth rates were observed under 5—20 % and 40 % volumes of the Dead Sea water (DSW). No difference was observed when we used 25— 35 % volumes of DSW. Strains from «Evolution Canyon» I grown at lower concentrations of the DSW revealed higher growth rates, while strains from the DSW demonstrated higher activity under higher stress (40 % volumes of DSW). Therefore, the following conclusion can be made: strains of A. niger isolated from the DSW are more adapted to stress associated with low-water activity.Сравнение темпов роста спор изолятов Aspergillus niger L. из контрастных по солености условий существования: гиперсоленых — Мертвого моря и умеренных — Европейского и Африканского склонов "Эволюционного каньона" (Израиль) дало возможность сделать вывод о большой адаптированности спор изолятов A. niger, выделенных из воды Мертвого моря, к стрессу, ассоциированному с низкой активностью воды.Порівняння темпів росту ізолятів Aspergillus niger з контрастних умов існування: гіпер-солоних — Мертвого моря і помірних — "Еволюційного каньйону" (Ізраїль) дало можливість дійти висновку про більшу адаптованість ізолятів A. niger, виділених з води Мертвого моря, до стресу, асоційованого з низькою активністю води
Changes of nucleic acids of wheat seedlings under spaceflight conditions
No full textThe effects of space flight on the growth of wheat seedlings and their nucleic acid content were studied. It was shown that both space and ground seedlings have almost the same appearance, dry weight and nucleic acid content in the root, coleoptile and leaves. The only difference found is in the RNA and DNA content, which is twice as much in the ground seedling apices as in the space-grown seedlings
The role of first Ukrainian Universities in the development of physiological investigation of plant growth and movement
No full textInformation about contribution at scientists of first Ukrainian Universities into development of plant physiology and biochemistry is presented. Special attention is paid to studies of plant growth and movement as a basis for formation of Kholodny-Went phytohormonal tropisms theory
SDS-PAGE of Ophioglossum vulgatum Proteins
No full textBackground: Despite that the protein composition is one of the main characteristics of plant organism, there are not many studies dedicated to the fern proteins characterisation. Protein composition as a part of biochemical characterisation of Ophioglossum vulgatum (an evolutionary distinct fern species related to Psilotaceae) is of interest from the point of view of ecology, taxonomy and plant biochemistry.
Aims: Study of the protein composition of roots and leaves of O. vulgatum during seasonal ontogenesis of its sporophytes.
Methodology: SDS-PAGE with following densitometry was used for the estimation of protein composition of roots and leaves of O. vulgatum collected from young plants, mature plants, spore-bearing and not spore-bearing plants at the peak of their seasonal development. To determine the phosphorylation of studied proteins the electrophoresis using Phos-tag™ Acrylamide Gels were applied.
Results: The simple and effective method of protein extraction from the leaves and roots of O. vulgatumwas developed and applied. Protein fractions with the molecular weights of 112±1.3, 57±1.0, 45±1.2, 35.5±0.6, 25.8±0.5, 18±1.5 kDa were shown as the major for both leaves and roots. Huge RuBisCO accumulation during the stages of plant initial growth and spore-bearing was shown (up to 55±10% in leaves and 89±5% in roots). All large chains of detected RuBisCO were phosphorylated which suggests that the active form of O. vulgatum RuBisCO is phosphoprotein.
Conclusion: The dynamic change of protein content was shown for different ontogenesis stages of O. vulgatum. The expression of phosphorylated RuBisCO was distinctly increased during the beginning of leaf development and spore-bearing
Genetic and epigenetic control of plant growth and development. Genes of auxin biosynthesis and auxin-regulated genes controlling plant cell division and extension
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