5 research outputs found
Dua Isolat Mangostin Dan Turunan Mangostin Termodifikasi Sebagai Agen Antidiabetes Dari Kulit Buah Garcinia mangostana Linn.
No full textGarcinia mangostana Linn. merupakan tumbuhan yang berasal dari famili Clusiaceae yang banyak mengandung metabolit sekunder terutama golongan turunan santon. Penelitian yang dilakukan telah berhasil mengisolasi dua turunan santon, yaitu 8-deoksigartanin (1) sebanyak 1,02 g (1,13%) dan β-mangostin (2) sebanyak 290 mg (0,32%), yang dihasilkan dari (90 g) ekstrak n-heksana kulit buah Manggis. Isolat β-mangostin (2) dilakukan modifikasi dengan pereaksi asam asetat anhidrida dihasilkan turunan 6-metil ester β-mangostin (3) sebanyak 34,2 mg (73%). Penentuan struktur terhadap ketiga senyawa tersebut dilakukan menggunakan metode spektroskopi UV-Vis, IR, HR-ESI-MS, dan NMR. Hasil uji aktivitas antidiabetes 6-metil ester β-mangostin (3) terhadap penghambatan α-glukosidase menunjukan nilai IC50 35,8 μM, hasil ini lebih aktif dibandingkan β-mangostin (2) dengan nilai IC50 = 157,9 μM. Berdasarkan data tersebut dapat dipastikan bahwa metode modifikasi dengan pereaksi asetat anhidrida dapat meningkatkan aktivitas antidiabetes senyawa.
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Garcinia mangostana Linn. is a plant comes from Clusiaceae family which contains many secondary metabolites, especially xanthones. Research has succeeded in isolating two xanthones, namely 8-Deoxygartanin (1) 1.02 g (1.13%) and β-mangostin (2) 290 mg (0.32%), resulting from (90 g) n-hexane extract of pericarp mangosteen. Isolate β-mangostin (2) was modified using acetic acid anhydride reagents produced 6-methyl ester β-mangostin (3) derivatives, 34.2 mg (73%). Determination of the structure of these three compounds was carried out using the UV-Vis, IR, HR-ESI-MS, and NMR spectroscopic methods. The results of antidiabetic activity of the 6-methyl ester β-mangostin (3) against α-glukosidase inhibition showed an IC50 value of 35,8 μM, this result was more active than β-mangostin (2) with an IC50 value of 157,9 μM. Based on these data, it is certain that the modification method with acetic anhydride reagents can increase the antidiabetic activity of the compound
Isolasi Dan Identifikasi Senyawa Metabolit Sekunder Ekstrak Kloroform Daun Tumbuhan Tembelekan (L. Camara Linn.)
Full text linkABSTRAKPenelitian ini bertujuan untuk mengisolasi dan mengidentifikasi senyawa metabolit sekunder yang terdapat dalam ekstrak kloroform daun tumbuhan tembelekan (L. Camara Linn.). Penelitian ini dilakukan melalui beberapa tahap diantaranya ekstraksi, fraksinasi, pemurnian dan identifikasi. Hasil penelitian diperoleh isolat berupa pasta berwarna putih sebanyak 3,15 mg, pada uji golongan dengan pereaksi Wagner terbentuk endapan cokelat dan pereaksi Mayer terbentuk endapan putih yang menunjukkan positif alkaloid. Spektrum FT-IR menunjukkan bilangan gelombang (cm-1) yakni, 3520.09 (N-H), 2922.16 dan 2852.72 (CH3 dan CH2), 1708.93 (C=O), dan 1463.97 (C=C). Identifikasi gugus fungsi senyawa dengan spektrofotometer FT-IR menunjukkan isolat memiliki kemiripan senyawa alkaloid.Kata kunci : L. Camara Linn, Wagner, Mayer, Alkaloid. ABSTRACTThe aim of this research was to identify the secondary metabolite compound by chloroform extract tembelekan’s plants leaf (L. Camara Linn). The isolation of secondary metabolite compound include: extraction, fractionation, purification, and identification. The research results obtained isolates form white pasta as much as 3.15 mg, on group test of the Wagner reactant gives the brown precipitate and Mayer reactant gives the white precipitate, it indicates positive alkaloids. FT-IR spectrum showing the number of waves, (cm-1) namely, 3520.09 (N-H), 2922.16 and 2852.72 (CH3 dan CH2), 1708.93 (C=O), dan 1463.97 (C=C). Identification the functional group of the compounds with FT-IR spectrophotometer showing the isolates have a similarity of indole alkaloid compounds.Keywords : L. Camara Linn, Wagner, Mayer, Alkaloid
Formulation of Lahuna Leave (Eupatorium odoratum) and Sirih Leave Extract (Piper betle L.) as Antiseptic Liquid Soap
Full text linkInfectious diseases caused by microorganisms are the main cause of high morbidity and mortality in the world. One of the plants that have the potential as an antiseptic is a lahuna leaf. Lahuna leaves contain active antibacterial compounds and the addition of betel leaf to the liquid soap formulations can strengthen the activity of the antiseptic produced. The purpose of this research is to test the effectiveness of liquid soap formulations of lahuna leaves and betel leaves as an antiseptic. The methods used include phytochemical screening, physical observation of liquid soap formulations, and antibacterial activity tests using agar diffusion methods. The results of the phytochemical analysis showed that lahuna leaves contain flavonoids, tannins, terpenoids, and alkaloids, while betel leaves contain flavonoids, saponins, tannins, terpenoids, and alkaloids. The liquid soap formulation of lahuna leaves and betel leaves has a clear yellow color, distinctive aroma, liquid form, rough taste, and lots of foam and has very strong inhibition against Staphylococcus aureus which is characterized by clear zones formed for each formulation I (22.4 mm), formulation II (21.8 mm) and formulation III (20.1 mm). These results indicate the potential of liquid soap formulations of lahuna leaves and betel leaves as antiseptic soap
Potential of Essential Oil Eucalyptus botryoides Leaves as an Antibacterial in Hand Sanitizer
Full text linkHand sanitizer is an antiseptic product that is widely used to prevent the spread of bacteria and viruses. Hand sanitizer preparations circulating in the market still use alcohol as an antibacterial agent. One of the natural antibacterial Eucalyptus is an antibacterial agent, and essential oil extracted from Eucalyptus can be named as natural ingredient. The purpose of this study was to determine the potential of essential oil E. botryoides leaves as an antibacterial ingredient to make of hand sanitizer. Eucalyptus essential oil is extracted from its leave using steam distillation method and formulated in the making of hand sanitizer gel with various concentrations of 2%, 4%, and 6%. The formulation of hand sanitizer gel was tested for antibacterial, organoleptic, homogeneity, pH, and dispersibility. The results showed that the more concentration of essential oil from Eucalyptus botryoides leaves added to the hand sanitizer gel, the more its antibacterial activity increased. The results of organoleptic observations, homogeneity, pH, and dispersibility of hand sanitizer gel preparations have met the requirements of SNI No.06-2588-1992. Thus, essential oil from E. botryoides leaves has potential as an antibacterial in hand sanitizer gel products
Isolation and antidiabetic Activity of Prenylated Xanthones from Pericarp of Mangosteen (Garacinia Mangostana Linn.)
Full text linkThe material used in this research was dry powder of pericarp mangosteen (Garcinia mangostana Linn.). which is a family plant of Clusiaceae. This taxa is known as the main source of prenylated xanthones derived from phenolic compounds. The compounds separation process was carried out by maceration method, 8 kg of sample macerated using n-hexane solvent, 90 g of n-hexane extract were produced. The n-hexane extract was fractionated by vacuum liquid chromatography (VLC) using silica gel, eluted by increasing the polarity of the solvent, which is a mixture of n-hexane solvent: dichloromethane (10%, 20%, 30% and 50%), resulting in three combined fractions, namely the first fraction 13,92 g, the second fraction 18,27 g, and the third fraction 23,24 g. The refraction process was then carried out in the third fraction, producing two yellow crystals with melting points respectively, 155-156 °C and 172-173 °C. The structural elucidation method was carried out using UV-Vis, IR, HR-ESI-MS, and NMR spectroscopy techniques. Based on the analysis of spectrum data from two known compounds, 8-deoxygartanin (1) and β-mangostin (2) antidiabetic bioactivity test was carried out by the method of inhibiting the enzyme α-glucosidase in vitro. The test results obtained by compound (1) with an IC50 value of 38,5 μM and compound (2) with an IC50 value of 157,9 μM indicate that the two compounds are included in the inactive category. This is indicated by the IC50 value which is much higher when compared to the acarbose as a positive control with an IC50 value of 4.5 μM
