34,924 research outputs found
The relationship of the ADP-ribosylating enzyme from S. solfataricus with DING proteins and its intracellular localization
No full textThe PARPSso thermoprotein from Sulfolobus solfataricus has been identified as a PARP-like enzyme that cleaves -NAD+ to synthesize oligomers of ADP-ribose and cross-reacts with polyclonal anti-PARP-1 catalytic site antibodies. Despite the biochemical properties that allow to correlate it to PARP enzymes, the N-terminal and partial amino acid sequence suggest the sulfolobal enzyme belongs to a different class of enzymes, the DING proteins. Considering the high sequence identity with the human DING protein HPBP and the lack of a nucleotide coding sequence in both human and sulfolobal genomes, we hypothesized that PARPSso might share other features with the human DING. Further analysis of PARPSso amino acid sequence addressed the research towards studying other possible similarities between human and sulfolobal protein and then to explain how PARPSso correlates with canonic PARPs. For the latter question, the peculiar behaviour of the thermozyme, that is biochemically, but not structurally related to the classic PARPs, stimulated to investigate by computational analysis and databank, whether the protein might be phylogenetically related to any already known PARP amino acid sequence.
Moreover, immunochemical and enzymatic crossed analyses were performed to establish whether purified HPBP and PARPSso have common immunoreactive and functional behaviour.
The second part of the research was focused on the localization of PARPSso within the sulfolobal cell. Our interest to this item arose from the property of some DING proteins to be membrane bound, suggested to work as membrane transporters. On the other hand, from previous studies, it is known that PARPSso is only partially solubilized from the starting cell homogenate provided by ICMIB (CNR), and the soluble enzyme is strictly associated with DNA. In this thesis work, whole cells collected by centrifugation from culture medium were subjected to a different extraction procedure. This procedure included also experimental conditions used to differentiate between soluble (i.e. cytoplasmic) and insoluble (i.e. membrane-bound) protein fractions. PARPSso and DNA distributions were determined by enzyme assay, immunoblotting and agarose gel electrophoresis. Reciprocal interactions of thermozyme, nucleic acid and membrane lipids were investigated with different techniques and methodologies (nucleoid preparation, fluorescence binding assays, fluorescence microscopy analysis)
Clues, flow channels, and cognitive states: an exploratory study of customer experiences with e-brokerage services
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A design for Six Sigma case study: creating an IT change management system for a mid-size accounting firm
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Characterizations of Ding Injective Complexes
No full text©2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
This document is the Accepted version of a Published Work that appeared in final form inBulletin of the Malaysian Mathematical Sciences Society. To access the final edited and published work see https://doi.org/10.1007/s40840-019-00807-8Let R be a ring and X a chain complex of R-modules. It is proven that if each term is Ding injective in R-Mod for all i in Z , and there exists an integer k such that each ZiX is Ding injective in R-Mod for all i>=k , then X is Ding injective in Ch(R) . If R is a left coherent ring, then a chain complex X is Ding injective if and only if each term is Ding injective in R-Mod for all i in Z
Introduction to the special section on cocreating the customer service experience with high tech and high touch
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Ding projective and Ding injective modules over trivial ring extensions
No full textsummary:Let be a trivial extension of a ring by an --bimodule such that , , and have finite flat dimensions. We prove that is a Ding projective left -module if and only if the sequence is exact and is a Ding projective left -module. Analogously, we explicitly describe Ding injective -modules. As applications, we characterize Ding projective and Ding injective modules over Morita context rings with zero bimodule homomorphisms
Optimal Consecutive-k-out-of-(2k+1): G Cycle
No full textWe present a complete proof for the invariant optimal assignment for consecutive-k-out-of-(2k+1): G Cycle, which was proposed by Zuo and Kao in 1990 with an incomplete proof, pointed out recently by Jalali, Hawkes, Cui, and Hwang.Du, Ding-Zhu; Hwang, Frank K.; Jung, Yunjae; Ngo, Hung Q.. (2000). Optimal Consecutive-k-out-of-(2k+1): G Cycle. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/215436
Author Correction: A Satellite Imagery Dataset for Long-Term Sustainable Development in United States Cities
No full textCorrection to: Scientific Data, published online 04 December 2023 In this article the author name Jingtao Ding was incorrectly written as Jintao Ding. The original article has been corrected.</p
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