14 research outputs found
A candid disquisition of the principles and practices of the Most Ancient and Honourable Society of Free and Accepted Masons : together with some strictures on the origin, nature, and design of the institution /
No full textESTCMode of access: Internet
A collection of thoughts, moral and divine, upon various subjects, in prose and verse. /
No full textPage [2] following p. xlvi and p. [1] at end blank.Errata: p. [1] following p. xlvi.ESTCMode of access: Internet
Automatic selection of partitioning schemes for phylogenetic analyses using iterative k-means clustering of site rates
No full textabstract: Background
Model selection is a vital part of most phylogenetic analyses, and accounting for the heterogeneity in evolutionary patterns across sites is particularly important. Mixture models and partitioning are commonly used to account for this variation, and partitioning is the most popular approach. Most current partitioning methods require some a priori partitioning scheme to be defined, typically guided by known structural features of the sequences, such as gene boundaries or codon positions. Recent evidence suggests that these a priori boundaries often fail to adequately account for variation in rates and patterns of evolution among sites. Furthermore, new phylogenomic datasets such as those assembled from ultra-conserved elements lack obvious structural features on which to define a priori partitioning schemes. The upshot is that, for many phylogenetic datasets, partitioned models of molecular evolution may be inadequate, thus limiting the accuracy of downstream phylogenetic analyses.
Results
We present a new algorithm that automatically selects a partitioning scheme via the iterative division of the alignment into subsets of similar sites based on their rates of evolution. We compare this method to existing approaches using a wide range of empirical datasets, and show that it consistently leads to large increases in the fit of partitioned models of molecular evolution when measured using AICc and BIC scores. In doing so, we demonstrate that some related approaches to solving this problem may have been associated with a small but important bias.
Conclusions
Our method provides an alternative to traditional approaches to partitioning, such as dividing alignments by gene and codon position. Because our method is data-driven, it can be used to estimate partitioned models for all types of alignments, including those that are not amenable to traditional approaches to partitioning.The electronic version of this article is the complete one and can be found online at: http://bmcevolbiol.biomedcentral.com/articles/10.1186/s12862-015-0283-
Transient Loss of Plasmid Mediated Mercuric Ion Resistance After Freezing and Thawing of Pseudomonas Auerginosa
No full textAuthor Institution: Department of Biological Sciences, Wright State UniversityAfter freezing and thawing, Pseudomonas aeruginosa harboring a drug resistance plasmid (Hg , Strep ), became acutely sensitive to mercuric ions but not to streptomycin in the plating medium. Its sensitivity to both agents in the plating medium became more pronounced, suggesting a synergistic effect of the mercuric ions with streptomycin. This freeze-thaw-induced sensitivity was transient and capable of being repaired in a simple salts medium (0.5%K2HPO4 + 0.04%MJSO4 pH7.7). Transient wall and membrane damage were also observed in frozen-thawed preparations. From kinetics studies, repair of membrane damage preceded repair of wall damage and damage measured by mercuric ions and mercuric ions plus streptomycin. Osmotically shocked cells also were sensitive to mercuric ions, mercuric ions plus streptomycin and sodium lauryl sulfate, but not to sodium chloride or streptomycin alone. This sensitivity was transient and capable of repair in the same simple salts medium. Active transport of a non-metabolisable amino acid, a-amino isobutyric acid, was sensitive to mercuric ions and became more so after freezing and thawing. A freeze-thaw resistant mercuric ion dependent NADPH oxido-reductase was localized in the cytoplasm, and the enzyme and an intact outer membrane appear to be required for mercuric ion resistance in this strain
Singing from the Grave: DNA from a 180 Year Old Type Specimen Confirms the Identity of Chrysoperla carnea (Stephens)
No full textCopyright: © 2015 Price et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The attached file is the published version of the article.NHM Repositor
New insights in high-energy electron emission and underlying transport physics of nanocrystalline Si
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Impact of COVID-19 on cardiovascular testing in the United States versus the rest of the world
No full textObjectives: This study sought to quantify and compare the decline in volumes of cardiovascular procedures between the United States and non-US institutions during the early phase of the coronavirus disease-2019 (COVID-19) pandemic.
Background: The COVID-19 pandemic has disrupted the care of many non-COVID-19 illnesses. Reductions in diagnostic cardiovascular testing around the world have led to concerns over the implications of reduced testing for cardiovascular disease (CVD) morbidity and mortality.
Methods: Data were submitted to the INCAPS-COVID (International Atomic Energy Agency Non-Invasive Cardiology Protocols Study of COVID-19), a multinational registry comprising 909 institutions in 108 countries (including 155 facilities in 40 U.S. states), assessing the impact of the COVID-19 pandemic on volumes of diagnostic cardiovascular procedures. Data were obtained for April 2020 and compared with volumes of baseline procedures from March 2019. We compared laboratory characteristics, practices, and procedure volumes between U.S. and non-U.S. facilities and between U.S. geographic regions and identified factors associated with volume reduction in the United States.
Results: Reductions in the volumes of procedures in the United States were similar to those in non-U.S. facilities (68% vs. 63%, respectively; p = 0.237), although U.S. facilities reported greater reductions in invasive coronary angiography (69% vs. 53%, respectively; p < 0.001). Significantly more U.S. facilities reported increased use of telehealth and patient screening measures than non-U.S. facilities, such as temperature checks, symptom screenings, and COVID-19 testing. Reductions in volumes of procedures differed between U.S. regions, with larger declines observed in the Northeast (76%) and Midwest (74%) than in the South (62%) and West (44%). Prevalence of COVID-19, staff redeployments, outpatient centers, and urban centers were associated with greater reductions in volume in U.S. facilities in a multivariable analysis.
Conclusions: We observed marked reductions in U.S. cardiovascular testing in the early phase of the pandemic and significant variability between U.S. regions. The association between reductions of volumes and COVID-19 prevalence in the United States highlighted the need for proactive efforts to maintain access to cardiovascular testing in areas most affected by outbreaks of COVID-19 infection
