1,721,489 research outputs found
Jae Ho Kim
No full text학위논문(박사)--아주대학교 일반대학원 :의학과,2010. 2TABLE OF CONTENTS
ABSTRACT ⅰ
TABLE OF CONTENTS ⅲ
LIST OF FIGURES ⅴ
ABBREVIATION ⅶ
Ⅰ. INTRODUCTION 1
Ⅱ. MATERIALS AND METHODS 3
1. Cell culture 3
2. In vivo protocol of experimental tumor models 3
3. In vitro and in vivo cell proliferation assays 4
4. In vivo analysis of tumor-tropism of human neural stem cells 5
5. X-gal stain 6
6. Cell count and unbiased stereology in a tumor region of rats 7
7 .Cell count and unbiased stereology in a entire brain of rats 8
8. Labeling of F3 Cells with 5-bromo-2-deoxyuridine 8
9. Hematoxylin and Eosin stain 9
10. Beta-galactosidase immunofluoresecence 9
11. Statistical Analysis 10
Ⅲ. RESULTS 11
1. Tumor-tropism pattern of NSCs 11
2. Migration rate of NSCs 19
3. NSC migration studied by stereology 23
4. Histological analysis 28
5. Proliferation of NSCs in vitro & in vivo 32
Ⅳ. DISCUSSION 37
Ⅴ. CONCLUSION 43
RFERENCES 45
국문요약 58Maste
Large-scale analysis of posttranslational modifications in the hippocampus of patients with Alzheimer's disease using pI shift and label-free quantification without enrichment
No full textPosttranslational modifications modulate protein
function in cells. Global analysis of multiple posttranslational
modifications can provide insight into physiology and disease,
but presents formidable challenges. In the present study, we
used a technique that does not require target enrichment to
analyze alterations in the phosphorylation and ubiquitination
of proteins from patients with Alzheimer’s disease (AD).
Guided by our previous findings, we applied three strategies
to further our understanding of the dysregulation of
posttranslationally modified proteins.We first identified phosphorylation
sites by determining peptide pI shifts using
OFFGEL. Second, using tandem mass spectrometry, we determined
the ubiquitination status of the proteins using an assay for a trypsin digestion remnant of ubiquitination (Gly-
Gly). Third, for large-scale discovery, we quantified the global
differences in protein expression. Of the proteins expressed in
AD tissue at levels of 2.0 or greater compared with controls,
60 were phosphorylated and 56 were ubiquitinated. Of the
proteins expressed at levels of 0.5 or lower compared with
controls, 81 were phosphorylated and 56 were ubiquitinated.
Approximately 98 % of the phosphopeptides exhibited a pI
shift.We identified 112 new phosphorylation sites (51.38 %),
and 92 new ubiquitination sites (96.84 %). Taken together, our
findings suggest that analysis of the alterations in
posttranslationally modified proteins may contribute to understanding
the pathogenesis of AD and other diseases.1451sciescopu
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Proteogenomics of the human hippocampus: The road ahead
No full textThe hippocampus is one of the most essential components of the human brain and plays an important role in learning and memory. The hippocampus has drawn great attention from scientists and clinicians due to its clinical importance in diseases such as Alzheimer's disease (AD), non-AD dementia, and epilepsy. Understanding the function of the hippocampus and related disease mechanisms requires comprehensive knowledge of the orchestration of the genome, epigenome, transcriptome, proteome, and post-translational modifications (PTMs) of proteins. The past decade has seen remarkable advances in the high-throughput sequencing techniques that are collectively called next generation sequencing (NGS). NGS enables the precise analysis of gene expression profiles in cells and tissues, allowing powerful and more feasible integration of expression data from the gene level to the protein level, even allowing -omic level assessment of PTMs. In addition, improved bioinformatics algorithms coupled with NGS technology are finally opening a new era for scientists to discover previously unidentified and elusive proteins. In the present review, we will focus mainly on the proteomics of the human hippocampus with an emphasis on the integrated analysis of genomics, epigenomics, transcriptomics, and proteomics. Finally, we will discuss our perspectives on the potential and future of proteomics in the field of hippocampal biology. This article is part of a Special Issue entitled: Neuroproteomics: Applications in Neuroscience and Neurology. © 2015 Elsevier B.V. All rights reserved19111sciescopu
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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