18 research outputs found
Adoption and Diffusion of Group Support Systems in Tanzania
No full textTechnology, Policy and Managemen
Scour depth development at piles of different height under the action of cyclic (tidal) flow
No full textThe impact of cyclic (tidal) flow on scour at cylindrical monopiles in the live-bed regime has been examined on the basis of results from mobile sand bed flume experiments. Four tests were conducted with four 0.114 m diameter piles of differing stickup height placed across the 4 m wide test section of the Fast Flow Facility. The tidal tests Tide01, Tide02 and Tide03 had the same peak current speed in forward and reverse direction with different cycle periods to examine the role of cycle length on scour. The period in Tide01 was approximately halved in Tide02 and doubled in Tide03. A unidirectional test Uni01 was run for comparative purposes. The results have shown how the cycle length adopted in testing has a key controlling effect on the scour achieved in a fixed number of cycles. The scour data measured on two sides of the piles at 1 Hz are plotted both in time-series format and pairwise to illustrate the cyclic nature of the scour development and recycling of sediment within the scour hole. The effective work method of Link et al. (2016) provides a good correlation for the dimensionless scour depth. The cyclic behaviour of the scour difference across the pile diameter has been analysed in terms of a basal slope and the magnitude of the scour depth, akin to geotechnical testing of monopile response to cyclic loads. Comparison with field measurements of scour shows that the cyclic tests can achieve non-dimensional scour depths found in the field. A progressive reduction in the slope angle is demonstrated for increasing number of cycles but further investigation of the different angle observed in field and laboratory is required. Finally the effect of pile stickup height is evaluated using both published and the current data, and a modified value of the Sumer and Fredsøe coefficient is proposed for undirectional and tidal scour
Emotional avatars:videos of animated emotional facial expressions
No full textThis collection of videos depicts emotional facial expression animations which were used as part of a doctoral research project. The animations include individual universal expressions of emotion, as well as transitions between emotions, where the timing of movements have been manipulated. These videos are being made freely available for use in other projects and research under a creative commons licence (CC BY-NC-ND-3.0) See http://creativecommons.org/licenses/by-nc-nd/3.0/. If the videos are used as part of any activity that leads to publication, you are requested to credit the author. The videos were discussed in: Sloan, R.J.S., Robinson, B., Scott-Brown, K., Moore, F. and Cook, M. 2010. Choreographing emotional facial expressions. Journal of Computer Animation and Virtual Worlds, 21(3-4), pp.203-213. DOI: 10.1002/cav.339
Emotional avatars:videos of animated emotional facial expressions
No full textThis collection of videos depicts emotional facial expression animations which were used as part of a doctoral research project. The animations include individual universal expressions of emotion, as well as transitions between emotions, where the timing of movements have been manipulated. These videos are being made freely available for use in other projects and research under a creative commons licence (CC BY-NC-ND-3.0) See http://creativecommons.org/licenses/by-nc-nd/3.0/. If the videos are used as part of any activity that leads to publication, you are requested to credit the author. The videos were discussed in: Sloan, R.J.S., Robinson, B., Scott-Brown, K., Moore, F. and Cook, M. 2010. Choreographing emotional facial expressions. Journal of Computer Animation and Virtual Worlds, 21(3-4), pp.203-213. DOI: 10.1002/cav.339
Efeitos de diferentes formulações comerciais de glyphosate sobre estirpes de Bradyrhizobium Effects of different glyphosate commercial formulations on Bradyrhizobium strains
No full textO objetivo deste trabalho foi avaliar efeitos de formulações comerciais de glyphosate sobre estirpes de Bradyrhizobium, em condições de laboratório. As formulações foram aplicadas na concentração de 43,2 µg L-1 do equivalente ácido. As bactérias foram inoculadas em meio de cultura à base de manitol e extrato de levedura (YM). O efeito do herbicida no crescimento das estirpes de Bradyrhizobium foi avaliado mediante leitura da densidade ótica em espectrofotômetro. Avaliou-se o crescimento das estirpes de B. japonicum SEMIA 5079 e de B. elkanii SEMIA 5019 e SEMIA 587 sob efeito de nove formulações de glyphosate: Zapp Qi®, Roundup®, Roundup Multiação®, Roundup Transorb®, Roundup WG®, Trop®, Agrisato®, glyphosate técnico [padrão de N-(phosphonomethyl) glycina] e controle sem adição de herbicida (testemunha para as estirpes). Foram utilizadas seis repetições. Confeccionaram-se curvas de crescimento para cada estirpe. Pelos resultados, pôde-se observar que todas as formulações de glyphosate causaram efeitos diferenciados sobre as estirpes de Bradyrhizobium SEMIA 5019, SEMIA 5079 e SEMIA 587. Constatou-se que a formulação Zapp Qi foi a menos tóxica às estirpes de Bradyrhizobium avaliadas. A maior toxicidade foi observada para Roundup Transorb, que provocou reduções no crescimento acima de 94% para todas as estirpes de Bradyrhizobium estudadas. Não se observou correlação entre o tipo de sal - isopropilamina, amônio ou potássico, presentes na formulação herbicida - e o grau de inibição no crescimento das estirpes. SEMIA 587 foi a estirpe menos tolerante à maioria das formulações testadas, porém SEMIA 5019 foi a mais sensível ao glyphosate padrão, sem adição de sais ou de outros aditivos.This work aimed to evaluate the effects of glyphosate commercial formulations on Bradyrhizobium strains under laboratory conditions. The formulations were applied in the concentration of 43.2 µg L-1 of the a.e. and the strains were inoculated in yeast extract manitol (YM). Herbicide effect on the growth of the Bradyrhizobium strains was assessed by optic density reading in a spectrophotometer. Twenty seven treatments arranged in a factorial design were evaluated and consisted of one strain of B. japonicum: SEMIA 5079; and two strains of B. elkanii: SEMIA 5019 and SEMIA 587, under the effect of nine glyphosate formulations: Zapp QI®, Roundup®, Roundup Multiação®, Roundup Transorb®, Roundup WG®, Trop®, Agrisato®, technical glyphosate [N-(phosphonomethyl) glycine] and control without herbicide addition (as the strain control treatment), with six replications. A growth curve was established for each strain. It could be observed that the different glyphosate formulations Zapp Qi, Roundup, Roundup Multiação, Roundup transorb, Roundup WG, Trop and Agrisato caused differentiated effects on the strains of Bradyrhizobium SEMIA 5019, SEMIA 5079 and SEMIA 587. It was verified that the Zapp Qi formulation was the least toxic to the strains. The highest toxicity was observed for Roundup Transorb, which reduced growth over 94% for all the strains assessed. Correlation was not observed among the type of salt, isopropylamine, ammonium or potassic, present in the formulation herbicides, and the toxicity degree to the strains. The strain SEMIA 587 was the least tolerant to most formulations while SEMIA 5019 was the most sensitive to the control treatment N- (phosphonomethyl) glycine, without salts or other additives
Eucalyptus Essential Oil As Bio-stimulator Of The Growth Of In Vitro Ectomycorrhizal Fungi [Óleo Essencial De Eucalipto Como Bioestimulador Do Crescimento De Fungos Ectomicorrízicos In Vitro]
No full textThe secondary metabolites extracted from forest species known as micossimbiontes can stimulate the growth of in vitro ectomycorrhizal isolates. We determined the effect of concentration of Eucalyptus grandis essential oil in stimulating growth of the ectomycorrhizal Pisolithus sp. (UFSC Pt 24 and UFSC Pt 188), Pisolithus microcarpus (UFSC Pt 116) Chondrogaster angustiporus (UFSC Ch 163), Scleroderma citrinum (UFSC Sc 124) and Suillus sp. (UFSM RA RA UFSM 2.2 and 2.8) in liquid culture medium. After a period of 25 days of incubation, we evaluated the morphology and growth of the isolates. The addition of essential oil at concentrations from 20 to 30 μL L-1 promoted mycelial growth in vitro in isolated Pt 24 UFSC, UFSC Pt 116, Ch 163 UFSC, UFSC Sc 124, UFSM RA RA UFSM 2.2 and 2.8. The addition of essential oil at a concentration of 20 μL L-1 resulted in an increase in the diameter and branching hyphae of isolates UFSC Pt 116 and Pt 24 UFSC. The use of essential oil in the preparation of culture media for the growth of ectomycorrhizal isolates has shown to be efficient and to increase the mycelial mass of the fungus.232405416Atanda, O.O., The potential of some species essential oils in the control of A. Parasiticus CFR 223 and aflatoxin production (2007) Food Control, 18 (5), pp. 601-607. , Reading, MayBâ, A.M., Management of ectomycorrhizal symbionts associated to useful exotic tree species to improve reforestation performances in tropical Africa (2010) Annals of Forest Science, 67 (3), pp. 298-307. , Champenoux, MayBakkali, F., Biological effects of essential oils - A review (2008) Food and Chemical Toxicology, 46 (2), pp. 446-475. , Richmond, FebBaptista, M.J., Produção de compostos fenólicos durante a infecção ectomicorrízica por dois isolados de Pisolithus tinctorius em Eucalyptus urophylla in vitro (1999) Revista Brasileira De Botânica, 22 (2), pp. 309-315. , São Paulo, outBaranska, M., Vibrational spectroscopic studies to acquire a quality control method of Eucalyptus essential oils (2005) Biopolymers, 78 (5), pp. 237-248. , Hoboken, AprBrimecombe, M.J., de Leij, F.A., Lynch, J.M., The effect of root exudates on rhizosphere microbial populations (2001) The Rhizosphere: Biochemistry and Organic Substances At the Soil-plant Interface, p. 424. , PINTON, R.VARANINI, Z.NANNIPIERI, P, New York: Marcel DekkerBrunner, I., Ectomycorrhizas: Their role in forest ecosystems under the impact of acidifying pollutants (2001) Perspectives In Plant Ecology, Evolution and Systematics, 4 (1), pp. 13-27. , Zürich, JuneBurgess, T., Effect of fungal-isolate aggressivity on the biosynthesis of symbiosisrelated polypeptides in differentiating eucalypt ectomycorrhizas (1995) Planta, 195 (3), pp. 408-417. , Bonn, JanCourty, P.E., The role of ectomycorrhizal communities in forest ecosystem processes: New perspectives and emerging concepts (2010) Soil Biology and Biochemistry, 42 (5), pp. 679-698. , Brisbane, MayDel Giudice, L., The microbial community of Vetiver root and its involvement into essential oil biogenesis (2008) Environmental Microbiology, 10 (10), pp. 2824-2841. , Braunschweig, OctDenny, H.J., Wilkins, D.A., Zinc tolerance in Betula spp. III. Variation in response to zinc among ectomycorrhizal associates (1987) New Phytologist, 106 (3), pp. 535-544. , LancasterEstanislau, A.A., Composição química e atividade antibacteriana dos óleos essenciais de cinco espécies de Eucalyptus cultivadas em Goiás (2001) Revista Brasileira De Farmacognosia, 11 (2), pp. 95-100. , Curitiba, jul./dezFabrowski, F.J., Investigação da presença de óleo essencial em Eucalyptus smithii R. T. Baker por meio da anatomia de seu lenho e casca (2003) Ciência Florestal, 13 (1), pp. 95-106. , Santa Maria, junFerreira, D.F., (2000) Sistemas De Análise Estatística Para Dados Balanceados, p. 145. , Lavras: UFLA/ DEX/SISVARFeugey, L., Induced defence responses limit Hartig net formation in ectomycorrhizal birch roots (1999) New Phytologist, 144 (3), pp. 541-547. , Lancaster, MarchForbes, V.E., Is hormesis an evolutionary expectation? (2000) Functional Ecology, 14 (1), pp. 12-24. , Amherst, FebGarcía, C., Bioxidation of 1,8-cineole by Aspergillus terreus (2009) Journal of Molecular Catalysis B: Enzimatic, 59 (1-3), pp. 171-176. , Delft, JulyHobbie, E.A., Carbon allocation to ectomycorrhizal fungi correlates with belowground allocation in culture studies (2006) Ecology, 87 (3), pp. 563-569. , Davis, MarHögberg, M.N., Högberg, P., Extramatrical ectomycorrhizal mycelium contributes one-third of microbial biomass and produces, together with associated roots, half the dissolved organic carbon in a forest soil (2002) New Phytologist, 154 (3), pp. 791-795. , June Lancaster(1991) User's Manual, p. 280. , JANDEL SCIENTIFIC, CaliforniaKainulainen, P., Holopainen, T., Holopainen, J.K., Decomposition of secondary compounds from needle litter of Scots pine grown under elevated CO2 and O3 (2003) Global Change Biology, 9 (2), pp. 295-304. , Illinois, FebKoide, R.T., Interactions between needles of Pinus resinosa and ectomycorrhizal fungi (1998) New Phytologist, 140 (3), pp. 539-547. , Lancaster, NovLagrange, H., Jay-Allgmand, C., Lapeyrie, F., Rutin, the phenolglycoside from eucalypt root exudates, stimulates Pisolithus hyphal growth at picomolar concentration (2001) New Phytologist, Lancaster, 149 (2), pp. 349-355. , FebLudley, K.E., Differential response of ectomycorrhizal and saprotrophic fungal mycelium from coniferous forest soils to selected monoterpenes (2008) Soil Biology & Biochemistry, 40 (3), pp. 669-678. , Brisbane, MarLudley, K.E., Potential for monoterpenes to affect ectomycorrhizal and saprotrophic fungal activity in coniferous forest is revealed by novel experimental system (2009) Soil Biology & Biochemistry, 41 (1), pp. 117-124. , Brisbane, JanMaffei, M.E., (2010) South African Journal of Botany, , Sites of synthesis, biochemistry and functional role of plant volatiles, Scottsville, In Press, Corrected Proof, Available online 8 AbrilMartin, F., The genome of Laccaria bicolor provides insights into mycorrhizal symbiosis (2008) Nature, 452 (7183), pp. 88-92. , London, MarMartins, M.A., de Gonçalves, G.F., Soares, A.C.F., Efeito De Fungos Micorrízicos Arbusculares Associados a Compostos Fenólicos, No Crescimento De Mudas De Mamoeiro (2000) Pesquisa Agropecuária Brasileira, 35 (7), pp. 1465-1471. , Brasília, julMarx, D.H., The influence of ectotrophic mycorrhizal fungi on the resistance of pine roots to pathogenic fungi and soil bacteria. I. Antagonism of mycorrhizal fungi to root pathogenic fungi and soil bacteria (1969) Phytopathology, 59, pp. 153-163. , Saint Paulde Matos, O.C., (2004) Substâncias Naturais De Origem Vegetal Com Actividade Biocida: Seu Uso Na Protecção Das Culturas, p. 14. , Lisboa: IIR PortugalMedice, R., Óleos essenciais no controle da ferrugem asiática da soja Phakopsora pachyrhizi Syd. & P. Syd (2007) Ciência E Agrotecnologia, 31 (1), pp. 83-90. , Lavras, jan./fevMenotta, M., Headspace solid-phase microextraction with gas chromatography and mass spectrometry in the investigation of volatile organic compounds in an ectomycorrhizae synthesis system (2004) Rapid Communications In Mass Spectrometry, 18 (2), pp. 206-210. , Jan, WashingtonMensen, R., Hager, A., Salzer, P., Elicitor - induced changes of wall - bound and secreted peroxidase activities in suspension - cultured spruce (Picea abies) cells are attenuated by auxins (1998) Physiologia Plantarum, 102 (4), pp. 539-546. , Lund, AprNehls, U., Fungal carbohydrate support in the ectomycorrhizal symbiosis: A review (2010) Plant Biology, 12 (2), pp. 292-301. , Freiburg, MarOliveira, V.L., Giachini, A.J., Ecologia e aplicação de ectomicorrizas (1999) Inter-relação Fertilidade, Biologia Do Solo E Nutrição De Plantas, p. 818. , SIQUEIRA, J. O. et al, UFLA: LavrasOluma, H.O.A., Garba, I.U., Screening of Eucalyptus globulus and Ocimum gratissimum against Pythium aphanidermatum (2004) Nigerian Journal of Plant Protection, 21, pp. 109-114Pera, J., Parladé, J., Inoculación controlada con hongos ectomicorrícicos en la producción de planta destinada a repoblaciones forestales: Estado actual en España (2005) Investigación Agraria: Sistemas Y Recursos Forestales, 14 (3), pp. 419-433. , MadridRossi, M.J., Souza, J.A.R., Oliveira, V.L., Inoculum production of the ectomycorrhizal fungus Pisolithus microcarpus in an airlift bioreactor (2002) Applied Microbiology and Biotechnology, 59 (2-3), pp. 175-181. , Münster, JulySalgado, S.M.L., Campos, V.P., Extratos naturais na patogenicidade e reprodução de Meloidogyne exigua em cafeeiro e de Meloidogyne incognita raça 3 em feijoeiro (2003) Nematologia Brasileira, 27 (1), pp. 41-48. , Piracicaba, junSalzer, P., Rapid reactions of spruce cells to elicitors released from the ectomycorrhizal fungus Hebeloma crustuliniforme, and inactivation of these elicitors by extracellular spruce cell enzymes (1996) Planta, 198 (1), pp. 118-126. , Jan. BonnSchawn-Estrada, K.R.F., Stangarlin, J.R., Cruz, M.E.S., (2003) Uso De Plantas Medicinais No Controle De Doenças De Plantas, pp. 54-56. , CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 36., 2003, Uberlândia. Anais... Uberlândia: SBFSerafini, L.A., Cassel, E., Produção de óleos essenciais: Uma alternativa para a agroindústria nacional (2001) Biotecnologia Na Agricultura E Na Agroindústria, pp. 333-377. , SERAFINI, L. A.BARROS, N. M.AZEVEDO, J. L, Guaíba: AgroindústriaShrivastava, G., Plant volatiles-based insect pest management in organic farming (2010) Critical Reviews In Plant Sciences, 29 (2), pp. 123-133. , Mar. ApopkaSimionatto, E., (2004) Estudo Dos Constituintes Químicos De Óleos Voláteis De Plantas Medicinais Do Rio Grande Do Sul: Isolamento, , Determinação E Modificação Estrutural E Atividade Biológica, 2004, 193 f. Tese (Doutorado em Química) - Universidade Federal de Santa Maria, Santa MariaSingh, G., Singh, O.P., Maurya, S., Chemical and biocidal investigations on essential oils of some indian Curcuma species (2003) Progress In Crystal Growth and Characterization of Materials, 45 (1-2), pp. 75-81. , JanSmith, S.E., Read, D.J., (2008) Mycorrhizal Symbiosis, p. 787. , 3rd ed., San Diego, Academic PressSoliman, K.M., Badeaa, R.I., Effect of oil extracted from some medicinal plants on different mycotoxigenic fungi (2002) Food and Chemical Toxicology, 40 (11), pp. 1669-1675. , Nov. Richmondde Souza, V.C., Estudos sobre fungos micorrízicos (2006) Revista Brasileira De Engenharia Agrícola E Ambiental, 10 (3), pp. 612-618. , Campina Grande, jul./setSteffen, R.B., Antoniolli, Z.I., Steffen, G.P.K., Efeito estimulante do óleo essencial de eucalipto na germinação e no crescimento inicial de mudas de Eucalyptus grandis (2010) Pesquisa Florestal Brasileira, 30 (63), pp. 199-206. , ColomboTaiz, L., Zeiger, E., (2004) Fisiologia Vegetal, p. 719. , 3. ed. Porto Alegre: ArtmedVitti, A.M.S., Brito, J.O., Universidade de São Paulo: Escola superior de Agricultura "Luiz de Queiroz" (2003) Óleo Essencial De Eucalipto, p. 26. , São Paulo, Documentos,17Zeppa, S., Tilia platyphyllos Scop.-Tuber brumale Vittad. vs. T. platyphyllos Scop.- T. borchii Vittad. ectomycorrhizal systems: A comparison of structural and functional traits (2005) Plant Physiology and Biochemistry, 43 (7), pp. 709-716. , Paris, Jul
Challenges in sexual and reproductive healthcare access for queer people in Gauteng, South Africa
No full textDATA AVAILABITY STATEMENT: The data that support the findings of this study are available
from the corresponding author, R.J.S., upon reasonable request.This article forms part of a special collection titled: Sexual Health.BACKGROUND: Sexual and reproductive healthcare services and needs (SRHS) are essential
for improving overall health and well-being. Queer people face obstacles not only in
obtaining and receiving SRHS but also in the provision of these services by healthcare
professionals (HCPs).
AIM: The study explored and described the challenges experienced by HCPs and queer people
when providing and accessing SRHS in the Gauteng province.
SETTING: We collected data from seven district public hospitals and two non-governmental
organisation clinics that focussed on queer-related healthcare in the Gauteng province,
South Africa.
METHODS: This was an explorative-descriptive qualitative study, in which we conducted
55 one-on-one interviews. The researchers employed purposive sampling to select all
HCPs, and respondent-driven sampling for all queer people who participated in the
study.
RESULTS: Six main themes emerged, demonstrating that HCPs and queer people faced
similar, contrasting and differing challenges when rendering and receiving SRHS. These
themes include HCPs’ belief that queer people are afraid, while queer people perceive
HCPs as having negative attitudes and acting as gatekeepers. HCPs expressed surprise
and confusion regarding gender identity, healthcare disparities and familial issues, which
highlighted their feelings of incompetence in providing queer-related healthcare and their
engagement with queer people as a barrier.
CONCLUSION: Policies need to be strengthened to ensure inclusivity in the healthcare sector,
thus, addressing SRHS for queer people.
CONTRIBUTION: The findings from this study have the potential to bridge discrepancies
and address the challenges faced by both HCPs and queer people through policy
development.The National Research Funding (NRF) and the Department of Higher Education (DHET) South Africa
through the new Generation of Academics Programme (nGAP).http://www.phcfm.orgInformaticsSDG-03:Good heatlh and well-bein
A microfluidic AFM cantilever based dispensing and aspiration platform
No full textWe present the development of a microfluidic AFM (atomic force microscope) cantilever-based platform to enable the local dispensing and aspiration of liquid with volumes in the pico-to-femtoliter range. The platform consists of a basic AFM measurement system, microfluidic AFM chip, fluidic interface, automated substrate alignment, external pressure control system and controlled climate near the dispensing area. The microfluidic AFM chip has a hollow silicon dioxide (SiO2) cantilever connected to an on-chip fluid reservoir at one end and a slicon nitride (Si3N4) tip with an aperture on the other end. A 3D printed plastic fluidic interface glued over the on-chip reservoir was used to connect microfluidics and macrofluidics. The fluidics is connected to an external pressure control system ranging from ?0.8 bar to 5 bar with 0.1 bar resolution. This pressure range allows dispensing and aspiration of liquids through the cantilever tip aperture. The controlled climate with a temperature control range between 25°C – 40°C and humidity up to 95% near the dispensing area keeps the droplets for sufficiently long time before they evaporate. An array of droplets can be programmed to be dispensed automatically and access them again with a position accuracy of 1 micron. Experiments were performed with two types of cantilevers with different geometrical configurations. A minimum flow rate control of 50 fL/s was obtained and also frequency shift was monitored as the cantilever was filled with liquid. This platform will be used for various chemical and biological applications.Precision and Microsystems EngineeringMechanical, Maritime and Materials Engineerin
Adaption of Hansenula polymorpha to methanol: A transcriptome analysis
No full textBackground: Methylotrophic yeast species (e.g. Hansenula polymorpha, Pichia pastoris) can grow on methanol as sole source of carbon and energy. These organisms are important cell factories for the production of recombinant proteins, but are also used in fundamental research as model organisms to study peroxisome biology. During exponential growth on glucose, cells of H. polymorpha typically contain a single, small peroxisome that is redundant for growth while on methanol multiple, enlarged peroxisomes are present. These organelles are crucial to support growth on methanol, as they contain key enzymes of methanol metabolism. In this study, changes in the transcriptional profiles during adaptation of H. polymorpha cells from glucose- to methanol-containing media were investigated using DNA-microarray analyses. Results: Two hours after the shift of cells from glucose to methanol nearly 20% (1184 genes) of the approximately 6000 annotated H. polymorpha genes were significantly upregulated with at least a two-fold differential expression. Highest upregulation (> 300-fold) was observed for the genes encoding the transcription factor Mpp1 and formate dehydrogenase, an enzyme of the methanol dissimilation pathway. Upregulated genes also included genes encoding other enzymes of methanol metabolism as well as of peroxisomal b-oxidation. A moderate increase in transcriptional levels (up to 4-fold) was observed for several PEX genes, which are involved in peroxisome biogenesis. Only PEX11 and PEX32 were higher upregulated. In addition, an increase was observed in expression of the several ATG genes, which encode proteins involved in autophagy and autophagy processes. The strongest upregulation was observed for ATG8 and ATG11. Approximately 20% (1246 genes) of the genes were downregulated. These included glycolytic genes as well as genes involved in transcription and translation. Conclusion: Transcriptional profiling of H. polymorpha cells shifted from glucose to methanol showed the expected downregulation of glycolytic genes together with upregulation of the methanol utilisation pathway. This serves as a confirmation and validation of the array data obtained. Consistent with this, also various PEX genes were upregulated. The strong upregulation of ATG genes is possibly due to induction of autophagy processes related to remodeling of the cell architecture required to support growth on methanol. These processes may also be responsible for the enhanced peroxisomal b oxidation, as autophagy leads to recycling of membrane lipids. The prominent downregulation of transcription and translation may be explained by the reduced growth rate on methanol (td glucose 1 h vs td methanol 4.5 h).Applied Science
Desenvolvimento de um modelo de simulação com base em conjuntos difusos, para avaliação da sustentabilidade da carcinicultura marinha em Santa Catarina
No full textTese (doutorado) - Universidade Federal de Santa Catarina, Centro Tecnológico
