53 research outputs found
Isolation And Characterization Of Polymorphic Microsatellite Loci For The Horn Fly, Haematobia Irritans (l.) (diptera: Muscidae)
No full textThe horn fly, Haematobia irritans (L.) (Diptera: Muscidae), is a cosmopolitan livestock pest that has caused a great negative impact on the animal production sector throughout the world. Here, we describe 10 polymorphic microsatellite loci isolated from H. irritans. The number of alleles found ranged from two to eight per locus and the expected heterozygosity from 0.1421 to 0.7702. These loci are potentially useful for the fine-scale genetic characterization of horn fly populations and provide fundamental information for pest management and planning of control programs. © 2008 The Authors.85971973Byford, R.L., Craig, M.E., Derouen, S.M., Al, E., Influence of permethrin, diazinon and ivermectin treatments on insecticide resistance in the horn fly (Diptera: Muscidae) (1999) International Journal of Parasitology, 29 (1), pp. 125-135Castiglioni, L., De Campos Bicudo, H.E., Molecular characterization and relatedness of Haematobia irritans (horn fly) populations, by RAPD-PCR (2005) Genetica, 124 (1), pp. 11-21Infante-Vargas, M.E., Azeredo-Espin, A.M.L., Genetic variability in mitochondrial DNA of screwworm, Cochiomyia hominivorax (Diptera: Calliphoridae), from Brazil (1995) Biochemical Genetics, 33, pp. 737-756Oliveira, M.T., De Azeredo-Espin, A.M., Lessinger, A.C., Evolutionary and structural analysis of the cytochrome c oxidase subunit I (COI) gene from Haematobia irritans, Stomoxys calcitrans and Musca domestica (Diptera: Muscidae) mitochondrial DNA (2005) DNA Sequence, 16 (2), pp. 156-160Oliveira, M.T., Da Rosa, A.C., Azeredo-Espin, A.M.L., Lessinger, A.C., Improving access to the control region and tRNA gene clusters of Dipteran mitochondrial DNA. (2006) Journal of Medical Entomology, 43 (3), pp. 636-639Oliveira, M.T., Azeredo-Espin, A.M.L., Lessinger, A.C., The Mitochondrial DNA Control Region of Muscidae Flies: Evolution and Structural Conservation in a Dipteran Context. (2007) Journal of Molecular Evolution, 64 (3), pp. 519-527Raymond, M., Rousset, F., Genepop (version 1.2): Population genetics software for exact tests and ecumenicism (1995) Journal of Heredity, 86 (3), pp. 248-249Rice, W.R., Analyzing tables of statistical tests (1989) Evolution, 43, pp. 223-225Rozen, S., Skaletsky, H.J., (1998) Primer 3, , http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi, Code available atSambrook, J., Maniatis, T., Fritsch, E.F., (1989) Molecular Cloning: A Laboratory Manual, , 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New YorkTorres, T.T., Azeredo-Espin, A.M.L., Development of new polymorphic microsatellite markers for the New World screw-worm Cochliomyia hominivorax (Diptera: Calliphoridae) (2005) Molecular Ecology Notes, 5, pp. 815-81
TaxMan: a taxonomic database manager
No full textAbstract Background Phylogenetic analysis of large, multiple-gene datasets, assembled from public sequence databases, is rapidly becoming a popular way to approach difficult phylogenetic problems. Supermatrices (concatenated multiple sequence alignments of multiple genes) can yield more phylogenetic signal than individual genes. However, manually assembling such datasets for a large taxonomic group is time-consuming and error-prone. Additionally, sequence curation, alignment and assessment of the results of phylogenetic analysis are made particularly difficult by the potential for a given gene in a given species to be unrepresented, or to be represented by multiple or partial sequences. We have developed a software package, TaxMan, that largely automates the processes of sequence acquisition, consensus building, alignment and taxon selection to facilitate this type of phylogenetic study. Results TaxMan uses freely available tools to allow rapid assembly, storage and analysis of large, aligned DNA and protein sequence datasets for user-defined sets of species and genes. The user provides GenBank format files and a list of gene names and synonyms for the loci to analyse. Sequences are extracted from the GenBank files on the basis of annotation and sequence similarity. Consensus sequences are built automatically. Alignment is carried out (where possible, at the protein level) and aligned sequences are stored in a database. TaxMan can automatically determine the best subset of taxa to examine phylogeny at a given taxonomic level. By using the stored aligned sequences, large concatenated multiple sequence alignments can be generated rapidly for a subset and output in analysis-ready file formats. Trees resulting from phylogenetic analysis can be stored and compared with a reference taxonomy. Conclusion TaxMan allows rapid automated assembly of a multigene datasets of aligned sequences for large taxonomic groups. By extracting sequences on the basis of both annotation and BLAST similarity, it ensures that all available sequence data can be brought to bear on a phylogenetic problem, but remains fast enough to cope with many thousands of records. By automatically assisting in the selection of the best subset of taxa to address a particular phylogenetic problem, TaxMan greatly speeds up the process of generating multiple sequence alignments for phylogenetic analysis. Our results indicate that an automated phylogenetic workbench can be a useful tool when correctly guided by user knowledge.</p
Plasma Pancreatic Lipase Activity: From Analytical Speeificity to Clinical Efficiency for the Diagnosis of Acute Pancreatitis
No full textSummary: Using five procedures (turbidimetry with the Boehringer Mannheim kit and with a home made reagent, Teflectometry with the Eastman Kodak kit, colorimetry with the Sigma kit, and UV spectrophotometry with the Wako kit), lipase activity was assayed in the same group of 60 healthy adults and in 30 patients suffering from acute pancreatitis (n = 197 samples) äs well äs in a purified and stabilized preparation of human pancreatic lipase. Results indicated considerable inter-assay discrepancies for the mean values of the patients ' results: catalytic activity concentrations differed by a factor of up to 16 according to the measurement procedures. For each method, mean i " patients ' results were also expressed äs multiples of the upper limit of normal values. This method of presentation | did not sigriificantly improve the intra-assay agreement, with maximal relative differences äs high äs 13-fold. When j each method was calibrated with the same material (human pancreatic lipase), the inter-assay agreement was! ' considerably improved. The causes of inter-assay disagreement are discussed in detail, and the necessity for a ' validated lipase calibrator is stressed, in order to improve the efficiency of the information transmitted by clinical laboratories to clinicians. A stfategy is proposed, which includes development of a reference method and reference \ material, and a study of inter-assay commutability of secondary calibrators for a set of methods. Introduction In this study, our goal was to: Pancreatic lipase (E.G. 3.1.1.3, triacylglycerol acylhyd- ~ compare the reaction conditions of the methods of rolase) is often considered to be a more sensitive and measurement commonly used in clinical laboratorie
Genetic Approaches For Studying Myiasis-causing Flies: Molecular Markers And Mitochondrial Genomics
No full text"Myiasis-causing flies" is a generic term that includes species from numerous dipteran families, mainly Calliphoridae and Oestridae, of which blowflies, screwworm flies and botflies are among the most important. This group of flies is characterized by the ability of their larvae to develop in animal flesh. When the host is a live vertebrate, such parasitism by dipterous larvae is known as primary myiasis. Myiasis-causing flies can be classified as saprophagous (free-living species), facultative or obligate parasites. Many of these flies are of great medical and veterinary importance in Brazil because of their role as key livestock insect-pests and vectors of pathogens, in addition to being considered important legal evidence in forensic entomology. 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Structure And Evolution Of The Mitochondrial Genomes Of Haematobia Irritans And Stomoxys Calcitrans: The Muscidae (diptera: Calyptratae) Perspective
No full textWe present the first two mitochondrial genomes of Muscidae dipterans for the species Haematobia irritans (the horn fly) and Stomoxys calcitrans (the stable fly). Typical insect mtDNA features are described, such as a high A + T content (79.1% and 78.9%, respectively), the preference for A + T-rich codons, and the evidence of a non-optimal codon usage. The strong A + T enrichment partially masks another nucleotide content bias maintained by A + C mutation pressure in these Muscidae mtDNAs. The analysis of this data provides a model of metazoans tRNA anticodon evolution, based on the selection hypothesis of anticodon versatility. H. irritans mitochondrial genome (16078 bp) is structurally similar to the hypothetical ancestral mitochondrial genome of arthropods and its control region (A + T-rich region in insects) organization is consistent with the structure described for Brachycera dipterans. On the other hand, the mitochondrial genome of S. calcitrans is ∼2 kb longer (18 kb), characterized by the presence of ∼550 bp tandem repeats in the control region, and an extra copy of trnI remarkably similar to a duplicated element of blowflies mtDNA. Putative sequence elements, involved in the regulation of transcription and replication of the mtDNA, were reliably identified in S. calcitrans control region despite the 0.8-1.5 kb gap uncovered from this genome. The use of amino acid and nucleotide sequences of concatenated mitochondrial protein-coding genes (PCGs) in phylogenetic reconstructions of Diptera does not support the monophyly of Muscomorpha, as well as the monophyly of Acalyptratae. Within the Calyptratae group, the inclusion of Muscidae (Muscoidea) as a sister group of Calliphoridae (Oestroidea) implies in a potential conflict concerning the monophyly of the superfamily Oestroidea. © 2008 Elsevier Inc. 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IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37 °C. Part 9 : reference procedure for the measurement of catalytic concentration of alkaline phosphatase International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) Scientific Division, Committee on Reference Systems of Enzymes (C-RSE) (1))
No full textAbstract This paper is the ninth in a series dealing with reference procedures for the measurement of catalytic activity concentrations of enzymes at 37 °C and the certification of reference preparations. Other parts deal with: Part 1. The concept of reference procedures for the measurement of catalytic activity concentrations of enzymes; Part 2. Reference procedure for the measurement of catalytic concentration of creatine kinase; Part 3. Reference procedure for the measurement of catalytic concentration of lactate dehydrogenase; Part 4. Reference procedure for the measurement of catalytic concentration of alanine aminotransferase; Part 5. Reference procedure for the measurement of catalytic concentration of aspartate aminotransferase; Part 6. Reference procedure for the measurement of catalytic concentration of γ-glutamyltransferase; Part 7. Certification of four reference materials for the determination of enzymatic activity of γ-glutamyltransferase, lactate dehydrogenase, alanine aminotransferase and creatine kinase at 37 °C; Part 8. Reference procedure for the measurement of catalytic concentration of α-amylase. The procedure described here is derived from the previously described 30 °C IFCC reference method. Differences are tabulated and commented on in Appendix 1
Class of 1963 - Day Section
No full textAaron, P. I. Bacharach, B. R. Bass, I. Benowitz, J. Berry, B. H. Bianchi, R. B. Billet, D. S. Carlin, J. L. Cassell, S. B. Celniker, P. Cherinsky, J. Chetkof, M. Cirigliano, C. D. Cohen, J. A. Colman, A. L. Conway, R. J. Cooper, A. G. Davies, R. C. Durso, S. A. Edelman, M. Edelstein, A. R. Eisenberg, G. Erlbaum, W. M. Esposito, S. E. Esser, G. I. Feder, L. H. Fisher, J. Flick, J. J. Forte, A. J. Franklin, G. B. Friedman, D. Fruchtman, B. M. Gallagher, W. J. Gandin, M. Gates, R. M. Gewirtz, S. Giorgini, A. J. Goldberg, S. B. Goldenberg, J. Goldstein, H. M. Graham, J. M., Jr. Green, A. B. Greenberg, A. I. Griffith, E. J. Grossman, M. B. Haber, G. Hashinsky, J. E. Hawxhurst, E. J. Hirschheimer, R. Horn, R. A. Hyman, F. C. Ioppolo, F. J. Jacobs, G. S. Janoff, L. D. Kalman, B. Kaplan, I. R. Kaplan, R. Kern, R. P. King, H. P. Klaris, H. J. Klein, H. L. Kluft, J. M. Koppell, E. G. Korman, G. Korn, J. H. Lampert, P. Landman, R. E. Leinheardt, W. L. Lessinger, B. Luboff, M. Lucas, R. G. Mandelbaum, R. I. Mandell, S. M. Mencher, B. D. Minkowitz, M. Muss, S. H. Nicoll, R. A. Nussbaum, R. C. Opal, J. E. Polansky, S. Quirindongo, F. J. Rapoport, J. Rosen, M. S. Rosenberg, J. J. Rosenbluth, S. Rosenhouse, H. Rubin, A. I. Rubinstein, R. Sakofsky, C. B. Saks, C. Sanzone, J. C. Schnaufer, J. C. Schneider, L. R. Schrager, D. L. Schwartz, I. A. Schwartz, R. J. Schwartzwald, E. L. Seidner, S. Seijas, J. Semel, M. Shapolsky, A. Shenkman, R. J. Sherman, S. C. Silkowitz, R. Slater, A. J. Slone, R. A. Slotnick, A. G. Stahl, E. G. Stander, R. M. Sullivan, C. H., Jr. Velcoff, J. I. Wank, M. H. Waxner, M. Weinsoff, H. Weinsoff, I. Weltz, A. A. Wendel, G. Winetsky, H. P. Winograd, E. C. Wiston, J. Zeelander, N. J. Ziskin, R. M. Zucker, C. I.https://brooklynworks.brooklaw.edu/bls_classphotos/1076/thumbnail.jp
Class of 1963 - Day Section
No full textAaron, P. I. Bacharach, B. R. Bass, I. Benowitz, J. Berry, B. H. Bianchi, R. B. Billet, D. S. Carlin, J. L. Cassell, S. B. Celniker, P. Cherinsky, J. Chetkof, M. Cirigliano, C. D. Cohen, J. A. Colman, A. L. Conway, R. J. Cooper, A. G. Davies, R. C. Durso, S. A. Edelman, M. Edelstein, A. R. Eisenberg, G. Erlbaum, W. M. Esposito, S. E. Esser, G. I. Feder, L. H. Fisher, J. Flick, J. J. Forte, A. J. Franklin, G. B. Friedman, D. Fruchtman, B. M. Gallagher, W. J. Gandin, M. Gates, R. M. Gewirtz, S. Giorgini, A. J. Goldberg, S. B. Goldenberg, J. Goldstein, H. M. Graham, J. M., Jr. Green, A. B. Greenberg, A. I. Griffith, E. J. Grossman, M. B. Haber, G. Hashinsky, J. E. Hawxhurst, E. J. Hirschheimer, R. Horn, R. A. Hyman, F. C. Ioppolo, F. J. Jacobs, G. S. Janoff, L. D. Kalman, B. Kaplan, I. R. Kaplan, R. Kern, R. P. King, H. P. Klaris, H. J. Klein, H. L. Kluft, J. M. Koppell, E. G. Korman, G. Korn, J. H. Lampert, P. Landman, R. E. Leinheardt, W. L. Lessinger, B. Luboff, M. Lucas, R. G. Mandelbaum, R. I. Mandell, S. M. Mencher, B. D. Minkowitz, M. Muss, S. H. Nicoll, R. A. Nussbaum, R. C. Opal, J. E. Polansky, S. Quirindongo, F. J. Rapoport, J. Rosen, M. S. Rosenberg, J. J. Rosenbluth, S. Rosenhouse, H. Rubin, A. I. Rubinstein, R. Sakofsky, C. B. Saks, C. Sanzone, J. C. Schnaufer, J. C. Schneider, L. R. Schrager, D. L. Schwartz, I. A. Schwartz, R. J. Schwartzwald, E. L. Seidner, S. Seijas, J. Semel, M. Shapolsky, A. Shenkman, R. J. Sherman, S. C. Silkowitz, R. Slater, A. J. Slone, R. A. Slotnick, A. G. Stahl, E. G. Stander, R. M. Sullivan, C. H., Jr. Velcoff, J. I. Wank, M. H. Waxner, M. Weinsoff, H. Weinsoff, I. Weltz, A. A. Wendel, G. Winetsky, H. P. Winograd, E. C. Wiston, J. Zeelander, N. J. Ziskin, R. M. Zucker, C. I.https://brooklynworks.brooklaw.edu/bls_classphotos/1076/thumbnail.jp
The mitochondrial DNA control region of muscidae flies: Evolution and structural conservation in a dipteran context
No full textThe structure and evolution of the mtDNA control region (CR) and its flanking genes in economically important dipterans from the family Muscidae (Brachycera: Calyptratae), Haematobia irritans, Musca domestica, Atherigona orientalis, and Stomoxys calcitrans are presented in this paper, along with the description of short noncoding intergenic regions possibly related to CR flanking sequences in Stomoxys calcitrans and Ophyra aenescens mtDNAs (ScIR and OaIR, respectively). S. calcitrans showed a large CR with an similar to 550-bp element tandemly repeated and a duplicated tRNA(Ile) stop gene. The characterization of H. irritans, M. domestica, A. orientalis, and S. calcitrans CR sequences led to the identification of seven conserved sequence blocks homologous to the elements previously described for Calliphoridae and Oestridae species (Brachycera: Calyptratae). Comparative analysis with Drosophila species (Brachycera: Acalyptratae) revealed four conserved regions. The putative functional roles of the conserved elements in the regulation of replication and transcription processes are addressed. The characterization of the structural organization of the mitochondrial genome CR demonstrates the plasticity of the mtDNA molecule in family Muscidae.64551952
Traceability of values for catalytic activity concentration of enzymes : a certified reference material for aspartate transaminase
No full textBackground: A new reference material for the liver enzyme aspartate transaminase (AST) (L-aspartate: 2-oxoglutarate-aminotransferase, EC 2.6.1.1), also called aspartate aminotransferase (ASAT), has been developed under the code ERM-AD457/IFCC. This certified reference material (CRM) for AST has been produced from a human type recombinant AST expressed in Escherichia coli and a buffer containing bovine serum albumin, and has been lyophilised.
Methods: The homogeneity and the stability of the material have been tested and the catalytic activity concentration has been characterised by 12 laboratories using the reference procedure for AST at 37 degrees C from the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC).
Results: The certified catalytic activity concentration and certified uncertainty of AST in the reconstituted material are (1.74 +/- 0.05) mkat/L or (104.6 +/- 2.7) U/L (with a coverage factor k = 2; 95% confidence interval).
Conclusions: Both the certified value and uncertainty are traceable to the International System of Units (SI). The material is aiming to control the IFCC reference procedure for AST at 37 degrees C, which will then be used to assign values to calibrants and control materials. The present paper highlights the scientific challenges and innovations which were encountered during the development of this new CRM
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