12 research outputs found
Induksi variasi somaklonal dan seleksi in vitro kalus embriogenik jahe (Zingiber officinale Rosc.) menggunakan filtrat bakteri ralstonia solanacearum untuk ketahanan terhadap bakteri layu
Get PDFPenyakit layu bakteri yang disebabkan oleh Ralstonia solanacearum merupakan salah satu penyakit penting pada tanaman jahe (Zingiber officinale Rosc.) yang seringkali menyebabkan kehilangan hasil. Berbagai teknik pengendalian, baik dengan menggunakan antibiotik (agrimicin, agrept), mikroba antagonis (Bacillus subtillis, Pseudomonas fluorescens, P. putida, P. cepacia) maupun kultur teknis anjuran, belum mampu mengatasi kendala tersebut secara optimal. Hal ini dikarenakan belum ada varietas jahe tahan terhadap R. solanacearum. Kendala utama untuk memperoleh varietas jahe tahan terhadap R. solanacearum adalah terbatasnya sumber gen ketahanan (narrow genetic stock) dan hambatan fisiologis karena adanya sifat inkompatibilitas sendiri (self-incompatibility), serta rendahnya fertilitas polen sehingga persilangan konvensional sulit dilakukan. Karena itu upaya untuk memperoleh varietas jahe tahan penyakit perlu dilakukan dengan meningkatkan ketahanan secara inkonvensional. Induksi variasi somaklonal dan seleksi in vitro kalus embriogenik menggunakan filtrat merupakan salah satu metode yang dapat digunakan untuk meningkatkan ketahanan. Tujuan Penelitian adalah ; (1) Mendapatkan konsentrasi filtrat R. solanacearum yang dapat menginduksi dan menyeleksi ketahanan kalus embriogenik jahe, dan (2) Mendapatkan varian kalus baru yang memiliki ketahanan terhadap filtrat R. solanacearum
STUDI PENDAHULUAN : INDUKSI KALUS EMBRIOGENIK DARI EKSPLAN DAUN Echinaceae purpurea
Get PDFEchinaceae is an introduced medicinal plant used to improve immune system of the body. Lately, interest on Echinaceae increased, however, good plant material for development is limited. One method to multiply plant material is the in vitro culture, so that research and development especially in vitro multiplication is required. The aim of the experiment is to procure a medium for the induction of embryonic callus and the technique of regeneration. The experiment is conducted at the Laboratory of Breeding and Genetic Resources, ISMECRI from January to December 2002, consisted of 2 steps, namely(1). Embryonic callus induction, (2). Callus regeneration. In the first step, explants were transferred into MS medium enrich with BA 0,1 mg/l + 2,4 D 0,5 mg/l and a combination medium of MS and LS medium supplemented with glutamine + BA (0,1 mg/l; 0,2 mg/l) + 2,4 D (0,5mg/l ; 1 mg/l). In the second step, regeneration was conducted on medium MS combined with LS + BA (0 mg/l + 0,2 mg/l + 0,4 mg/l), MS combined with LS + Kinetine (0,2 mg/l; 0,4 mg/l). The results showed that embryonic callus was obtained from culture of leaf explants on MS medium combined with LS. The best treatment for step regeneration was MS combined LS + BA 0,4 mg/l for shoot formation while MS combined LS + kinetine 0,4 mg/l induced root formation.
Pengaruh Umur Eksplan Terhadap Keberhasilan Pembentukan Kalus Embriogenik Pada Kultur Meristem Jahe (Zingiber Officinale Rosc)
Get PDFKendala dalam pengembangan jahe di Indonesia adalah terbatasnyabenih bermutu. Secara konvensional, budidaya jahe dilakukan denganmenggunakan bibit dari potongan rimpang. Dengan cara ini diperlukanbibit dalam jumlah yang banyak, antara 2-3 t/ha untuk jahe yang dipanentua dan 5-6 t/ha untuk yang dipanen muda. Kendala lain adalah penyakittular benih layu bakteri yang disebabkan oleh Ralstonia solanacearum.Salah satu upaya yang dapat dilakukan untuk mendapatkan benih jahebebas penyakit adalah perbanyakan melalui kultur jaringan. Penelitianbertujuan untuk mengkaji sumber eksplan dari tingkat umur panenrimpang yang berbeda terhadap kapasitas pembentukan kalus embriogenikpada kultur meristem jahe putih besar. Penelitian dilakukan di BalaiPenelitian Tanaman Obat dan Aromatik dari September 2007 sampaiMaret 2008, menggunakan rancangan acak lengkap dengan 20 kaliulangan. Bahan tanaman yang digunakan adalah meristem jahe putih besaryang diambil dari rimpang panen muda dan tua. Peubah yang diamatimeliputi: histologi jaringan, persentase kalus embriogenik yang terbentuk,bobot segar kalus, diameter kalus, dan morfologi kalus. Hasil penelitianmenunjukkan adanya daerah meristematik pada sayatan eksplan meristemjahe putih besar ukuran ± 0,25 cm. Persentase kalus embriogenik (92,1%)dan diameter kalus (0,59 mm) dari rimpang yang dipanen tua lebih tinggidari yang dipanen muda. Berat kalus (1,18 g) dan jumlah embrio somatikglobular (29,34) asal eksplan panen tua nyata lebih tinggi dari yangdipanen muda. Kalus embriogenik yang berasal dari eksplan rimpang yangdipanen tua mampu berkembang membentuk embrio somatik danberkecambah menghasilkan planlet normal
EFISIENSI MEDIA KULTUR DAN APLIKASI TEMPORARY IMMERSION SYSTEM PADA EMBRIOGENESIS SOMATIK KOPI ARABIKA / Efficiency of Culture Media and Aplication Temporary Immersion Systemon on Somatic Embryogenesis Arabica Coffee
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The Induction of Primary and Secondary Somatic Embryo to Support Arabica Coffee Propagation
No full textThe primary and secondary somatic embryogenesis can be used to propagate Coffea arabica L clonally. However, the success of this propagation was depended on plant growth regulator and varieties. This study aimed to examine the possibility of 2,4-D and thidiazuron application to form primary and secondary somatic embryo to support Arabica coffee clonal propagation. The study consisted of two activities (1) 2,4-D and thidiazuron Application to Induce Primary Somatic Embryogenesis of Arabica Coffee and (2) The Application of thidiazuron in Solid and Semi-Solid Media to Induce Secondary Somatic Embryos. The results indicated significant effect of varieties and plant growth regulator on fresh weight, number of torpedo and germinated embryo. However, it showed no significant effect on callus formation percentage. The best medium to induce primary somatic embryogenesis depending on variety, on the treatment of 4.52 μM 2,4 -D +18.16 μM thidiazuron was the best for AS2K and Sigarar Utang varieties, S 795 at 4.52 μM 2,4-D + 9.08 μM thidiazuron, whereas Kartika at 4.52 μM 2.4-D + 13.62 μM thidiazuron. The morphology of coffee somatic embryo was normal. Primary somatic embryo was developed indirectly, whereas the secondary somatic embryo was directly. The application of 9.08 μM thidiazuron increased the percentage and number of secondary somatic embryos, hence enhancing number of Arabica coffee planlet. Keywords : Coffea arabica L, 2,4-D, thidiazuron, semi-solid media, Indirect somatic embryogenesi
Yield performance and stability analysis of three cultivars of Gayo Arabica coffee across six different environments
No full textThe three cultivars of Gayo Arabica coffee (GAC) are distributed widely in the Gayo Highlands, Aceh Province, Indonesia, between 900 and 1,700 m above sea level (masl). The difference in altitude affects the yield and quality of coffee, and Arabica coffee has biennial bearing characteristics, so there are always annual yield fluctuations. This study aimed to determine the yield performance and stability level of the three GAC cultivars, Gayo 1, Gayo 2, and Gayo 3 (G3), across six different environments. The study used a randomized complete block design with three replications. The six environmental conditions are the combinations of two altitudes (900 and 1,500 masl) and 3 years of production (2019, 2020, and 2021). The estimation of coffee yields is based on the fruit value, which is calculated by the number of productive branches per tree, nodes per branch, berries per node, and the weight of a single cherry. An investigation of the genotype-by-environmental interaction using a combined analysis of variance and the yield performance and stability analysis performed using the Eberhart and Russell method, AMMI (additive main effects and multiplicative interaction) and GGE (genotype + genotype × environment) biplot analysis. Results showed that the yield performance of GAC was significantly affected by the interaction between genotype and environment. G3 was an ideal cultivar because it had high yield performance, was stable, and could adapt to broader environments in Gayo Highland. Therefore, G3 deserves high priority for Arabica coffee development in that region
INDUKSI EMBRIO SOMATIK SEKUNDER KOPI ARABIKA DAN DETEKSI KERAGAMAN SOMAKLONAL MENGGUNAKAN MARKA SSRs / Induction of Secondary Somatic Embryos of Arabica Coffee and Detection Somaclonal Variation Using SSRs Marker
Get PDFThe secondary somatic embryogenesis of coffee plant can be used to propagate superior varieties, plant resulted from genetic transformation and mutation. Present study aimed to obtain the best media composition for induction of secondary somatic embryos in solid or semi-solid media, and to evaluate the possibility of somaclonal variations occurrence in the resulting plantlets. Primary somatic embryos torpedo phase of the AS2K variety were used as explant sources. Types of cytokines i.e. 2-iP (4.54 and 9.08 μM), kinetin (9.30 μM) and BAP (BAP 17.76 and 1.33 μM) and medium density (solids and semi-solid) were used as treatments. A total of 20 SSRs marker were used in molecular analysis of plantlets with 10 replication per treatment. The results showed that the media with the addition of BAP 17.76 μM resulted in the highest percentage (75.50%), the highest number of secondary somatic embryos (10.63), the tertiary, quarter and quiner somatic embryos. Number of secondary somatic embryos produced in a dense media was higher than those in the semi-solid media.. Based on molecular analysis, planlets on all treatment were relatively homogenous except on medium with 17.76 μM BAP which indicated by one allelle changing at ssrR209 locus. These findings indicated that the use of culture medium with supplemented with 9.08 μM 2-iP is advisable to induce the secondary somatic embryos due to its capacity to produce high number of somatic embryos and exhibited no somaclonal variations occurred among the plantlets.Keywords: Coffea arabica, tertiary somatic embryos, quarter somatic embryos, quiner somatic embryos, semi solid media AbstrakEmbriogenesis somatik sekunder pada tanaman kopi dapat digunakan untuk memperbanyak varietas unggul, hasil transformasi dan mutasi. Penelitian ini bertujuan untuk mendapatkan komposisi media terbaik dalam menginduksi embrio somatik sekunder dalam media padat maupun semi padat, dan mengevaluasi kemungkinan terjadinya variasi somaklonal pada planlet yang dihasilkan. Eksplan yang digunakan adalah embrio somatik primer fase torpedo dari varietas AS2K. Perlakuan yang diuji adalah jenis sitokinin yaitu: 2-iP (4,54 dan 9,08 μM), kinetin (9,30 μM) dan BAP (BAP 17,76 μM dan 1,33 μM) dan kepadatan media (padat dan semi padat). Analisis molekuler menggunakan 20 primer marka SSRs dengan jumlah ulangan 10 planlet per perlakuan. Hasil penelitian memperlihatkan media dengan penambahan BAP 17,76 μM menghasilkan persentase embrio somatik sekunder tertinggi (75,50%), embrio somatik sekunder terbanyak (10,63), embrio somatik tersier, kuarter dan kuiner. Jumlah embrio somatik sekunder yang dihasilkan pada media padat lebih banyak dibandingkan semi padat. Berdasarkan analisis molekuler, planlet yang dikulturkan pada semua perlakuan relatif seragam, kecuali pada perlakuan BAP 17,76 μM yang menunjukkan telah terjadi perubahan satu alel pada lokus ssrR209. Temuan ini memperlihatkan bahwa penggunaan media kultur dengan 2-iP 9,08 μM lebih dianjurkan untuk menginduksi embrio somatik sekunder karena dapat menghasilkan jumlah embrio somatik cukup banyak dan tidak memperlihatkan adanya variasi somaklonal pada planlet yang dihasilkan.Kata kunci: Coffea arabica, embrio somatik tersier, embrio somatik kuarter, embrio somatik kuiner, media semi-pada
POTENSI BAKTERI ENDOFIT MENGINDUKSI KETAHANAN TANAMAN LADA TERHADAP INFEKSI Meloidogyne incognita
Get PDFABSTRAKMeloidogyne incognita, merupakan salah satu organisme peng-ganggu (OPT) penyebab penyakit kuning pada tanaman lada dan dapatmengakibatkan penurunan hasil sampai 32%. Beberapa teknik untukmengendalikan patogen ini telah dilakukan tetapi belum memberikan hasilyang memuaskan. Pengendalian biologi dengan menggunakan bakteriendofit merupakan salah satu alternatif pengendalian yang cukup men-janjikan untuk dapat mengatasi permasalahan nematoda penyakit tanaman.Penelitian ini telah dilakukan di Laboratorium Bakteriologi danNematologi Departemen Proteksi Tanaman Institut Pertanian Bogor, danRumah Kaca Balai Penelitian Tanaman Rempah dan Aneka TanamanIndustri Pakuwon Sukabumi dari bulan Mei sampai November 2009.Kegiatan yang dilakukan adalah: 1) Seleksi beberapa isolat bakteri endofituntuk mengendalikan nematoda M. incognita pada tanaman lada dan 2)Potensi induced systemic resistance (ISR) dan analisis asam salisilat sertaperoksidase. Isolat bakteri endofit yang digunakan adalah isolat bakteriendofit potensial yang diisolasi dari akar nilam. Akar tanaman ladadirendam dalam suspensi bakteri endofit, selanjutnya diinokulasi dengan500 ekor larva 2 M. incognita. Sebulan setelah inokulasi tanamandibongkar diamati populasi nematoda dan pertumbuhan tanaman. AnalisisISR dilakukan dengan metode split root system dilanjutkan dengananalisis kadar asam salisilat dan peroksidase. Penelitian mengunakanRancangan Acak Lengkap. Hasil penelitian menunjukkan bahwa bakteriendofit dapat menekan jumlah puru dan populasi nematoda di dalam akar.Penekanan tertinggi pada isolat MSK (97,93%) tidak berbeda nyatadengan isolat BAS, TT2, dan NJ46 yaitu 97,35; 95,22; dan 92,14%.Berdasarkan analisis split root system, ke 4 isolat tersebut dapat meng-induksi ketahanan tanaman lada secara sistemik dengan mekanismepeningkatan kandungan asam salisilat dan peroksidase di dalam akar.Kata kunci : Bakteri endofit, penyakit kuning, Piper nigrum L.,Meloidogyne incognita, induksi ketahananABSTRACTThe use of endophytic bacteria to induce plant resistanceagainst infection of root-knot nematode (Meloidogyneincognita) on black pepperRoot-knot nematode (Meloidogyne incognita) is one of important patho-gens causing yellow disease on black pepper. As a result of this pathogenattack can lower the results up to 32%. Several control methods have beendone successful to control pathogen. Biological control using endophyticbacteria is one of prospective alternative control methods to overcomenematode problem. The research had been conducted in the Laboratory ofBacteriology and Nematology Department of Plant Protection, BogorAgricultural University (IPB) and in greenhouse of Indonesian Spices andIndustrial Crops Research Institute (ISICRI) Sukabumi. The objectives ofthis study were : 1) Selection of endophytic bacteria to control M.incognita nematodes on black pepper and 2) Potential of induced systemicresistance (ISR) and analysis of salicylic acid and peroxidase. Endophyticbacterial isolates used were endophytic potential bacterial isolates isolatedfrom the roots of patchouli. Pepper plant roots were soaked in anendophytic bacterial suspension, then inoculated with 500 larvae of 2 M.incognita. A month after inoculation, the plants were dismantled andobserved population of nematodes and plant growth. ISR analysis wasperformed by the method of split root system followed by analysis ofsalicylic acid and peroxidase contents. The research was arranged usingCompletely Randomized Design. The results showed that endophyticbacteria were able to suppress the amount of gall and nematode populationin roots. The highest suppression was on MSK isolate (97.93%) which wasnot significantly different from BAS, TT2, and NJ46 isolates, namely97.35, 95.22, and 92.14%, respectively. The analysis of split root systemshowed that the 4 isolates were able to induce systemic resistance of blackpepper with a mechanism of increase in salicylic acid and peroxidasecontents in roots.Key words : Endophytic bacteria, yellow disease, Piper nigrum L.,Meloidogyne incognita, induce systemic resistanc
Small-scale coffee farmers’ perception of climate-adapted attributes in participatory coffee breeding: A case study of Gayo Highland, Aceh, Indonesia
No full textSmall-scale coffee farming in Indonesia faces low productivity due to poor farming practices and low-quality planting materials. It highlights the need for improved coffee varieties that meet farmers’ preferences. Given the vulnerability of coffee farming to climate change, participatory breeding programs that involve collaboration between researchers and farmers to develop a climate-adapted variety are essential. This study used survey data from Gayo Highland, Aceh, Indonesia, to investigate farmers’ perception of the importance of climate-related attributes in a coffee variety, including those developed through a participatory breeding program, and the determinant factors. The result shows that farmers rated climate-related attributes as the least important (average score 0.36) compared to others, such as coffee productivity (1.57) and quality (1.22), resistance to pests and diseases (0.96), and input-use efficiency (0.57). This finding suggests a lack of awareness among farmers about the importance of climate issues in coffee farming. The estimation results also indicate that coffee extension activities have a negative association with farmers’ perceptions of the importance of climate attributes. This study recommends inclusive and targeted climate campaigns to increase farmers’ concern, awareness, and knowledge about the threats of climate change to coffee farming through strengthening advisory services
