Buletin Penelitian Tanaman Rempah dan Obat
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Isolation and Identification of Antioxidant Compounds 2-Ethylhexyl-4-methoxycinnamate from Avocado Seed Extract (Persea americana Mill.)
Full text linkAlpukat (Persea americana Mill.) merupakan tanaman yang banyak ditemukan di daerah tropis seperti Indonesia. Selama ini masyarakat hanya memanfaatkan daging buahnya saja untuk dikonsumsi, kemudian bagian bijinya dibuang. Penelitian ini bertujuan untuk mengisolasi, menguji dan mengidentifikasi senyawa aktif antioksidan dari ekstrak biji alpukat yang dilakukan dari Januari sampai Juni 2018 di Laboratorium Kimia Bahan Alam, Pusat Penelitian Bioteknologi-LIPI. Buah alpukat yang digunakan dalam penelitian diperoleh dari Pasar Induk Cibitung, Bekasi. Ekstraksi dilakukan secara bertingkat menggunakan pelarut n-heksana, etil asetat, etanol 96%, dan air. Uji aktivitas antioksidan dilakukan dengan metode peredaman radikal bebas menggunakan reagen 2,2-difenil-1-pikrilhidrazil (DPPH), penentuan struktur kimia senyawa aktif antioksidan menggunakan metode spektrofotometri UV-Vis, Spektroskopi Inframerah Transformasi Fourier (FTIR), dan GCMS. Hasil pengujian aktivitas antioksidan menunjukkan bahwa ekstrak etanol 96% memiliki aktivitas antioksidan tertinggi pada isolat EtOH.4.3.1 sebagai isolat paling aktif dengan IC50 sebesar 23,07±1,63 μg.ml-1. Hasil identifikasi berdasarkan data spektrofotometri UV-Vis, Spektroskopi Inframerah Transformasi Fourier, dan GCMS menunjukkan bahwa isolat EtOH.4.3.1 biji alpukat mengandung senyawa 2-etilheksil-4-metoksisinamat, yang terbukti memiliki aktivitas sebagai antioksidan. Analisis lebih lanjut perlu dilakukan menggunakan spektrometri Resonansi Magnetik Inti (RMI) proton dan karbon untuk memastikan jumlah dan posisi proton serta karbon dalam struktur kimia tersebut.Avocado (Persea americana Mill.) is a plant widely found in tropical regions such as Indonesia. Currently, people only consume the avocado flesh and dispose of the seed. This study aimed to examine the antioxidant activity, isolate and identify the active antioxidant structure from avocado seed extracts. The trial was conducted from January to June 2021 at the Chemical Laboratory of Natural Substance, Research Center for Biotechnology, LIPI. The avocado fruit used in this research was obtained from Cibitung Central Market, Bekasi. The solvents used in the extraction were n-hexane, ethyl acetate, 96% ethanol, and water with multilevel extraction. Antioxidant activity was tested using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical method. The structure of the active compound was determined using UV-Vis spectrophotometry, Fourier Transformation Infrared Spectroscopy (FTIR), and GCMS. The 96% ethanol extract had the highest antioxidant activity with EtOH.4.3.1 isolate as the most active antioxidant isolate with IC50 23,07±1,63 μg.ml-1. The identification results of isolate EtOH.4.3.1 of avocado seed extract with UV-Vis spectrophotometry, FTIR, and GCMS indicated 2-Ethylhexyl-4-methoxycinnamate acting as an antioxidant. Further analysis with Nuclear Magnetic Resonance (NMR) spectrometry of protons and carbon is required to determine the number and position of protons and carbon in the chemical structure
Effect of Organic Fertilizer and Biofertlizer Combination on the Growth, Yield and Quality of Gotu Kola
Full text linkPegagan merupakan salah satu tanaman obat yang sudah mulai dibudidayakan secara intensif oleh masyarakat Indonesia. Budidaya pegagan perlu dilakukan dengan menerapkan Good Agricultural Practice untuk menghasilkan bahan baku terstandar termasuk penggunaan pupuk. Pupuk hayati telah banyak dikembangkan untuk mengurangi efek buruk penggunaan pupuk buatan terhadap lingkungan, sehingga budidaya tanaman secara organik menjadi salah satu alternatif. Penelitian ini bertujuan, mendapatkan pupuk organik terbaik untuk pertumbuhan, hasil dan mutu pegagan. Penelitian ini merupakan penelitian eksperimental menggunakan Rancangan Acak Lengkap (RAL), 3 ulangan dengan 8 perlakuan media tanam yang terdiri dari: (a) Tanah + pupuk organik + dolomit; (b) Tanah + pupuk organik + pupuk hayati 1 g; (c) Tanah + dolomit + pupuk hayati 1 g; (d) Tanah + pupuk organik + pupuk hayati 3 g; (e) Tanah + dolomit + pupuk hayati 3 g; (f) Tanah + pupuk organik + pupuk hayati 5 g; (g) Tanah + dolomit + pupuk hayati 5 g; dan (h) Tanah + pupuk hayati 3 g. Parameter pengamatan meliputi luas daun, panjang tangkai, jumlah daun, jumlah anakan, berat brangkasan basah, berat brangkasan kering dan kandungan asiatikosid. Hasil penelitian menunjukkan bahwa perlakuan media tanah + pupuk organik (1,5 kg) + dolomit (30 g) memberikan pengaruh yang lebih baik untuk pertumbuhan, produksi herba dan produksi asiatikosid pada pegagan. Perlakuan pupuk hayati (1 g) dengan kombinasi dolomit (30 g) memberikan pengaruh lebih baik dalam peningkatan jumlah anakan dan jumlah daun, sedangkan kombinasi pupuk hayati (5 g) dan dolomit (30 g) terbaik dalam kadar asiatikosid pada pegagan.Gotu kola is one of the medicinal plants that has begun to be cultivated by the Indonesian people. It’s cultivation needs to follow the GAP to produce the qualified yield, including the use of fertilizers. Biofertilizers have been widely developed to reduce adverse effects of chemical fertilizers to the environment, thus an organic cultivation method can be an alternative. The aim of this study was to determine the effect of biofertilizer on growth and yield of gotu kola. This research was an experimental study with a completely randomized design (CRD) using 8 planting media treatments consisting of (a) Soil + manure + dolomite; (b) Soil manure + 1 g biofertilizer; (c) Soil + dolomite + 1g biofertilizer; (d) Soil + manure + 3 biofertilizer; (e) Soil + dolomite + 3 biofertilizer; (f) Soil + manure + 5 g biofertilizer; (g) Soil + dolomite + 5 g biofertilizer; and (h) Soil + 3 g biofertilizer. Parameters observed were leaf area, stem length, number of leaves, number of tillers, fresh weight, dry weight and asiaticoside content. The results showed that combination treatment of biofertilizer (1 g) with dolomite (30 g) gives better effect in increasing the number of tillers and number of leaves, while the combination of biofertilizer (5 g) and dolomite (30 g) is the best for asiaticoside levels in gotu cola
Efek Imunomodulasi Ekstrak Etanol Kaempferia galanga terhadap Proliferasi Sel Limfosit Secara In Vitro
Full text linkKaempferia galanga L. is a traditional medicine with antitumor properties, as indicated by its immunomodulatory activities. This study aimed to determine the effect of K. galanga on lymphocyte cell proliferation activity as an indicator of immunomodulatory properties. This study was conducted at the Indonesian Research Center for Veterinary Science (February to April 2018). The immunomodulatory activity of the extract was evaluated with an in vitro splenocyte proliferation assay. The assay was based on cellular enzymatic synthesis to transform the XTT from formazan tetrazolium as an indicator. The K. galanga extract was obtained by 96% ethanol extraction. The test was conducted in an aseptic condition, consisted of five treatment groups with three replications each. Three groups of splenocyte cell culture, each with extract concentration of 2.5 µg.ml-1, 25 µg.ml-1, and 250 µg.ml-1, as well as a positive (Concanavalin A/Con A) and negative (cell only) control. The cell suspension (10x104 cells/ml) was distributed on 96-well plates and cultured following the treatment groups. The same five plates were made for five days of observation and retrieved daily by observing an Elisa reader at 450 nm. The extract of K. galanga at 2.5 µg.ml-1, 25 µg.ml-1, and 250 µg.ml-1 significantly (P <0.05) promoted splenocyte proliferation compared to control. Therefore, it was expected that K. galanga has a high potential to be used as immunomodulators. Hence, further investigations should be done to clarify the mechanisms of the immunomodulatory effect of K. galanga as an antitumor in vivo.Kencur (Kaempferia galanga L.) merupakan obat tradisional yang dikenal bermanfaat sebagai antitumor yang diindikasikan dengan aktivitas imunomodulator. Penelitian ini bertujuan untuk mengobservasi pengaruh ekstrak etanol K. galanga terhadap aktivitas proliferasi sel limfosit sebagai indikator aktivitas imunomodulator. Penelitian dilakukan di Balai Besar Penelitian Veteriner Bogor mulai Februari sampai April 2018. Aktivitas imunomodulator dievaluasi dengan cara menguji kemampuannya dalam meningkatkan proliferasi splenosit in vitro. Metode ini menguji kemampuan enzim dalam sel untuk mentransformasi garam tetrazolium dari XTT menjadi produk berwarna biru yaitu formazan. Ekstrak kencur yang digunakan dalam penelitian ini diekstraksi dengan menggunakan etanol 96%. Uji dilakukan secara aseptis terdiri lima kelompok perlakuan masing-masing dengan tiga ulangan, tiga kelompok dengan ekstrak kencur pada konsentrasi 2,5 µg.ml-1, 25 µg.ml-1, dan 250 µg.ml-1, dan dua kelompok sebagai kontrol positif (Concanavalin A/Con A) dan negatif (hanya sel splenosit). Uji ini menggunakan 10 x 104 sel/ml dalam media RPMI yang dimasukkan ke dalam lempeng sel kultur yang terdiri dari 96 lubang dan dikulturkan sesuai kelompok perlakuan. Selanjutnya, dibuat 5 lempeng yang sama untuk pengamatan selama 5 hari. Setiap hari diambil 1 lempeng untuk diukur serapannya pada panjang gelombang 450 nm. Hasil penelitian menunjukkan adanya peningkatan aktivitas proliferasi sel limfosit pada kelompok yang diberi ekstrak kencur yang berbeda nyata (P<0,05) jika dibandingkan dengan kelompok tanpa ekstrak kencur. Dengan demikian, hasil penelitian ini menunjukkan bahwa kencur berpotensi untuk dikembangkan sebagai imunomodulator. Oleh karena itu, penelitian lebih lanjut tentang aktivitas imunomodulasi kencur yang berperan dalam eliminasi tumor secara in vivo perlu dilakukan
KOMPATIBILITAS EKSTRAK Piper retrofractrum Vahl. DAN Curcuma xanthorrhiza Roxb. UNTUK PENGENDALIAN Helopeltis antonii Sign.
Full text linkPlant extracts as a botanical insecticide can be used in either single or mixture forms. Mixed extracts have advantages: reducing dependence on one plant species, being the synergistic effect which can increase the effectiveness of formulas, and reducing the possibility of resistance emergence. This study aimed to obtain compatible and synergistic comparisons of P. retrofractum with C. xanthorrhiza extracts that effectively control the third instar nymph of Helopeltis antonii. The comparison of P. retrofractum and C. xanthorrhiza extracts were 4:1, 2:1, 1:1, 1:2, and 1:4 (w/w). The experiment was performed in a completely randomized design using the contact method. The LC50, LC90, and LC95 values were calculated using the PoloPlus Ver 1.0 Program. The mixture interaction was determined from the Combination Index value. The results showed that P. retrofractum extract was compatible and synergistic with C. xanthorrhiza extract at a ratio of 2:1 (w/w). These were the best combination shown by the lowest LC50, LC90, and LC95 values and showed consistently strong synergistic properties compared to other concentrations. The components of P. retrofractum may be responsible for the insecticidal properties are piperine; piperanine; piperidine; 3,4-Pyridinedimethanol,6-methyl; Piperidine,1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]-,(Z,Z)-; and (2E,4E,12E)-1-(Piperidine-1-yl)octadeca-2,4,12—trien-1-one. The insecticidal activity of C. xanthorrhiza may associated with its content of xanthorrhizol, Ar-curcumene, β-curcumene, curzerene, germacrone, 7-zingiberene, caryophyllene, and β-bisabolol. A combination of these two plant extracts has the potential to be further developed as a botanical insecticide to control H. antonii.Ekstrak tanaman sebagai bahan insektisida nabati dapat digunakan secara tunggal maupun campuran. Penggunaan campuran ekstrak memiliki kelebihan, antara lain dapat mengurangi ketergantungan pada satu jenis/spesies tumbuhan, efek sinergisme yang ditunjukkan dapat meningkatkan efektivitas formula dan memperlambat kemungkinan terjadinya resistensi serangga. Penelitian ini bertujuan untuk mendapatkan perbandingan ekstrak P. retrofractum yang kompatibel dan sinergis dengan ekstrak C. xanthorrhiza yang efektif untuk mengendalikan nimfa instar ketiga Helopeltis antonii. Perbandingan ekstrak P. retrofractum dan C. xanthorrhiza pada uji kompatibilitas, yaitu 4:1, 2:1, 1:1, 1:2 dan 1:4 (w/w). Pengujian dirancang secara acak lengkap menggunakan metode kontak serangga. Nilai LC50, LC90 dan LC95 dihitung dengan menggunakan Program PoloPlus Ver 1.0. Sifat interaksi campuran ditentukan dari nilai Indeks Kombinasi (IK). Hasil penelitian menunjukkan ekstrak P. retrofractum kompatibel dan bersifat sinergis secara konsisten dengan ekstrak C. xanthorrhiza pada perbandingan 2:1 (w/w). Perbandingan tersebut merupakan kombinasi terbaik yang ditunjukkan oleh nilai LC50, LC90 dan LC95 yangpaling rendah dan menunjukkan sifat sinergis kuat secara konsisten dibandingkan dengan perbandingan lain yang diuji. Komponen ekstrak etil asetat P. retrofractum yang diduga bersifat insektisida, di antaranya piperin, piperanin, piperidin, 3,4-Pyridinedimethanol, 6-methyl-; Piperidine,1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]-,(Z,Z)-; dan (2E,4E,12E)-1-(Piperidin-1-yl)octadeca-2,4,12—trien-1-one. Senyawa metabolit sekunder pada ekstrak etanol C. xanthorrhiza yang diduga memiliki aktivitas insektisida, antara lain xanthorrhizol, Ar-curcumene, β-curcumene, curzerene, germacrone, 7-zingiberene, caryophyllene, dan β-bisabolol. Kombinasi kedua ekstrak tanaman ini berpotensi untuk dikembangkan lebih lanjut sebagai insektisida nabati untuk mengendalikan H. antonii
Genetic Diversity of 64 Turmeric Accessions from Indonesia Based on P450-Based Analogue (PBA) Marker
Full text linkKunyit merupakan tanaman penghasil rimpang yang memiliki banyak kegunaan, baik untuk konsumsi, industri obat, maupun pewarna. Pengembangan varietas unggul kunyit di Indonesia saat ini perlu didukung oleh adanya informasi keragaman genetik. Saat ini informasi mengenai keragaman genetik tanaman kunyit di Indonesia masih belum tersedia. Salah satu cara untuk memperoleh informasi keragaman genetik adalah dengan menggunakan marka molekuler yang mampu memberikan hasil yang akurat dan tidak dipengaruhi oleh lingkungan. Marka PBA sebagai marka fungsional mampu mendeteksi gen P450 yang berkaitan dengan pembentukan metabolit sekunder pada area genom yang luas sehingga dapat dijadikan alternatif marka untuk mengidentifikasi keragaman genetik. Tujuan dari penelitian ini adalah untuk memperoleh informasi keragaman genetik 64 aksesi tanaman kunyit menggunakan delapan pasang primer P450-Based Analogue (PBA). Penelitian dilakukan di Laboratorium Sentral Universitas Padjadjaran dari Juni 2019 hingga Januari 2020. Sebanyak 133 pita terdeteksi dengan rentang jumlah masing-masing alel 8 – 45 pita, dan rata-rata per alel 22,3 pita. Hasil analisis PIC menunjukkan adanya enam pasang primer PBA yang menunjukkan polimorfisme tinggi pada rentang 0,90 – 0,98 sehingga marka PBA dikategorikan sangat informatif. Analisis klaster membagi 64 aksesi kunyit ke dalam dua klaster utama berdasarkan tingkat kemiripan pada rentang 0,01 hingga 0,83. Aksesi CL-GTL01 yang berasal dari Gorontalo memiliki kemiripan yang rendah yaitu 0,01 terhadap 64 aksesi lainnya, sedangkan aksesi CL-NTB01 dan CL-PPB04 memiliki tingkat kemiripan yang tinggi pada jarak 0,83. Berdasarkan nilai PIC, jumlah pita polimorfik, dan jarak genetik, kunyit asal Indonesia memiliki keragaman yang luas berdasarkan marka PBA. Turmeric is a rhizome producing plant with many utilization such as for consumption, medicine, and colorant industries. The development of superior turmeric varieties in Indonesia needs to be supported by genetic diversity information availability. Despite its potential, genetic diversity information of Indonesian turmeric has not been widely observed. A molecular marker is used to address genetic diversity information with the accurate result due to minimum environmental influences. PBA can detect the P450 gene as a functional marker, which is related to the synthesis of secondary metabolites in a wide genome area. Thus, it can be used as an alternative marker to identify genetic diversity. This research aimed to obtain genetic diversity information of 64 turmeric accessions using eight primer sets of P450-Based Analogue (PBA). The study was conducted in the Central Laboratory of Padjadjaran University from June 2019 to January 2020. Results showed that the full 133 bands were detected with a range of allele number 8 - 45 bands and an average of 22.3 bands per allele. PIC analysis showed six primer sets of PBA had high polymorphisms ranged from 0.90 to 0.98, hence categorized PBA as a highly informative marker. Cluster analysis divided 64 turmeric accessions into two main clusters based on a similarity index ranged from 0.01 to 0.83. The accession of CL-GTL01 origins from Gorontalo had a low similarity coefficient of 0.01 to the other 64 accessions cluster. On the other hand, CL-NTB01 dan CL-PPB01 had the highest similarity index of 0.83. Based on the PIC value, the total number of polymorphic bands, and genetic distance, it can be concluded that local Indonesian turmeric had wide diversity based on PBA marker
DNA Barcoding of Medicinal Orchid Dendrobium discolor Lindl. Tanimbar Using rbcL and ITS genes
Full text linkDendrobium discolor Lindl., Tanimbar is one of the medicinal orchids that has been used to treat skin diseases. Morphologically, D. discolor Tanimbar shows similarities with D. discolor Merauke and D. bigibbum, making it challenging to identify. DNA barcoding using ribulose-1,5-bisphosphate carboxylase large (rbcL) and Internal Transcribed Spacer (ITS) markers expected to be used to identify D. discolor accurately. This study aimed to identify potential DNA sequences as barcodes for the identification of medicinal orchid D. discolor Tanimbar using molecular markers rbcL and ITS. The DNA genome of D. discolor Tanimbar was isolated and used as a template in the PCR reaction. The resulting amplicons were then sequenced. The results showed that the rbcL gene sequence of D. discolor had high homology with D. salaccense (Accession: LC193510.1, Prect. Ident : 99.45 %), whereas the ITS had high homology with D. nindii (Accession: AY239985.1 Identification: 98.67 %). Bioinformatics analysis showed that the rbcL gene sequence from D. discolor had more homology sequence than the ITS. However, the ITS sequence was more specific and could differentiate to species level. Based on the results of this study, the ITS sequence can be recommended as a molecular marker for the identification of the medicinal orchid D. discolor Tanimbar
Effect of Physiological Maturity and Germinating Media on Zanzibar Clove Seeds Viability
Full text linkLow seeds quality of the clove tree may attribute to the low national productivity of cloves in Indonesia. The study aimed to determine the level of physiological fruit maturity and the effect of germination media on the viability of clove seeds. The study was performed at the Leuwikopo Seeds Storage and Quality Testing Laboratory and Leuwikopo Research Installation, Bogor, in October 2017 to December 2018. The material used was the Zanzibar clove seeds from ≥ 10 years old trees. The clove seeds obtained from a farmer’s plantation in Tolitoli District, Central Sulawesi. The study arranged in a split-plot design, with four replications, with the main plot was four levels of seed viability based on fruit colors: reddish-green, pink, red, and dark red. The subplots were three types of germination media, namely sand, cocopeat, and zeolite. Thus, there were twelve treatments. Each plot consisted of 40 seeds for germination test and five seeds for water content determination. The results showed that the percentage of clove seeds viability negatively influenced by the level of fruit maturity based on the color of the fruit but was affected by the germination media. Good physiological quality of clove seeds characterized by dry seed weight of 2.70 g, 100 % germination rate, vigor index of 73.75 %, and growth rate of 1.19 % etmal-1. Among the three types of germination media tested (sand, cocopeat, and zeolite), sand was the best germination media for clove seeds. Hence it is recommended to seeds producer.Low seeds quality of the clove tree may attribute to the low national productivity of cloves in Indonesia. The study aimed to determine the level of physiological fruit maturity and the effect of germination media on the viability of clove seeds. The study was performed at the Leuwikopo Seeds Storage and Quality Testing Laboratory and Leuwikopo Research Installation, Bogor, in October 2017 to December 2018. The material used was the Zanzibar clove seeds from ≥ 10 years old trees. The clove seeds obtained from a farmer’s plantation in Tolitoli District, Central Sulawesi. The study arranged in a split-plot design, with four replications, with the main plot was four levels of seed viability based on fruit colors: reddish-green, pink, red, and dark red. The subplots were three types of germination media, namely sand, cocopeat, and zeolite. Thus, there were twelve treatments. Each plot consisted of 40 seeds for germination test and five seeds for water content determination. The results showed that the percentage of clove seeds viability negatively influenced by the level of fruit maturity based on the color of the fruit but was affected by the germination media. Good physiological quality of clove seeds characterized by dry seed weight of 2.70 g, 100 % germination rate, vigor index of 73.75 %, and growth rate of 1.19 % etmal-1. Among the three types of germination media tested (sand, cocopeat, and zeolite), sand was the best germination media for clove seeds. Hence it is recommended to seeds producer
Aktivitas In Vitro Penurun Gula Darah dari Ekstrak Buah Parijoto (Medinilla speciosa B.)
Full text linkDiabetes mellitus is one of the highest causes of death in the world, with symptoms of increased blood glucose levels (hyperglycemia). One of the efforts made as a treatment and prevention of DM is through complementary therapy using natural ingredients. Parijoto fruit (Medinilla speciosa) contains flavonoid compounds potential to reduce glucose levels. The study aimed to analyze the effectiveness of ethanol extracts and its fractions of parijoto fruit (EEBP) to decrease glucose levels in vitro. The study was conducted at the Ngudi Waluyo University Laboratory in April to August 2018. Identification and screening of flavonoid compounds from ethanol extracts and its fractions of parijoto fruit were performed qualitatively and by thin-layer chromatography (TLC). In vitro testing for antidiabetic activity of EEBP was performed with non-enzymatic reaction by Nelson Somogyi method (ethanol extract and its fractions). Experiment was arranged in a randomized block design, with 12 treatments and three replications. The concentrations of parijoto fruit tested were 10 ppm, 20 ppm, 30 ppm, 40 ppm, 50 ppm, and 60 ppm. The results of qualitative identification and TLC showed that the ethanol extract and its fraction of EEBP contained flavonoid compounds. At a concentration of 30 ppm, EEBP was able to reduce glucose levels by 42.43 %, while the ethanol fraction decreased glucose levels by 83.38 %. The results showed parijoto fruit ethanol extract and its fractions were sources of antidiabetic. Hence its pharmacologically effect was necessary to be further studied.Penyakit diabetes melitus (DM) merupakan salah satu penyebab kematian tertinggi di dunia dengan gejala peningkatan kadar glukosa (hiperglikemia) dalam darah. Salah satu upaya yang dilakukan sebagai pengobatan dan pencegahan DM adalah melalui terapi komplemeter menggunakan bahan alam. Buah parijoto (Medinilla speciosa) mengandung senyawa flavonoid yang berpotensi dalam menurunkan kadar glukosa. Tujuan penelitian adalah menganalisis keefektifan ekstrak dan fraksi etanol buah parijoto (EEBP) terhadap penurunan kadar glukosa secara in vitro. Penelitian dilaksanakan di Laboratorium Universitas Ngudi Waluyo sejak April sampai Agustus 2018. Identifikasi dan skrining senyawa flavonoid dari ekstrak dan fraksi etanol buah parijoto dilakukan secara kualitatif dan kromatografi lapis tipis (KLT). Aktifitas antidiabetes EEBP diuji secara invitro dengan metode non-enzimatis Nelson-Somogyi (ekstraksi dan fraksi etanol) menggunakan rancangan acak kelompok, 12 perlakuan dengan tiga ulangan. Konsentrasi buah parijoto yang diuji adalah 10 ppm, 20 ppm, 30 ppm, 40 ppm, 50 ppm, dan 60 ppm. Hasil identifikasi kualitatif dan KLT menunjukkan bahwa ekstrak dan fraksi etanol EEBP mengandung senyawa flavonoid. Pada konsentrasi 30 ppm, EEBP mampu menurunkan kadar glukosa sebesar 42,43 %, sedangkan fraksi etanolnya menurunkan kadar glukosa 83,38 %. Penelitian ini menunjukkan bahwa ekstrak dan fraksi etanol buah parijoto berpotensi sebagai sumber antidiabetes, namun perlu diteliti lebih lanjut secara farmakologis
