19,606 research outputs found
Multiple functions of LIM domain-binding CLIM/NLI/Ldb cofactors during zebrafish development
No full textThe crucial involvement of CLIM/NLI/Ldb cofactors for the exertion of the biological activity of LIM homeodomain transcription factors (LIM-HD) has been demonstrated. In this paper we show that CLIM cofactors are widely expressed during zebrafish development with high protein levels in specific neuronal cell types where LIM-HD proteins of the Isl class are synthesized. The overexpression of a dominant-negative CLIM molecule (DN-CLIM) that contains the LIM interaction domain (LID) during early developmental stages of zebrafish embryos results in an impairment of eye and midbrain-hindbrain boundary (MHB) development and disturbances in the formation of the anterior midline. On a cellular level we show that the outgrowth of peripheral but not central axons from Rohon Beard (RB) and trigeminal sensory neurons is inhibited by DN-CLIM overexpression. We demonstrate a further critical role of CLIM cofactors for axonal outgrowth of motor neurons. Additionally, DN-CLIM overexpression causes an increase of Isl-protein expression levels in specific neuronal cell types, likely due to a protection of the DN-CLIM/LIM-HD complex from proteasomal degradation. Our results demonstrate multiple roles of the CLIM cofactor family for the development of entire organs, axonal outgrowth of specific neurons and protein expression levels
Characterization of the <i>P-t</i><sub><i>lim</i></sub> relationship.
No full text<p>Characterization of the <i>P-t</i><sub><i>lim</i></sub> relationship.</p
Four and a half LIM protein 1C (FHL1C)
No full textFour-and-a-half LIM domain protein 1 isoform A (FHL1A) is predominantly expressed in skeletal and cardiac muscle. Mutations in the FHL1 gene are causative for several types of hereditary myopathies including X-linked myopathy with postural muscle atrophy (XMPMA). We here studied myoblasts from XMPMA patients. We found that functional FHL1A protein is completely absent in patient myoblasts. In parallel, expression of FHL1C is either unaffected or increased. Furthermore, a decreased proliferation rate of XMPMA myoblasts compared to controls was observed but an increased number of XMPMA myoblasts was found in the G(0)/G(1) phase. Furthermore, low expression of K(v1.5), a voltage-gated potassium channel known to alter myoblast proliferation during the G(1) phase and to control repolarization of action potential, was detected. In order to substantiate a possible relation between K(v1.5) and FHL1C, a pull-down assay was performed. A physical and direct interaction of both proteins was observed in vitro. In addition, confocal microscopy revealed substantial colocalization of FHL1C and K(v1.5) within atrial cells, supporting a possible interaction between both proteins in vivo. Two-electrode voltage clamp experiments demonstrated that coexpression of K(v1.5) with FHL1C in Xenopus laevis oocytes markedly reduced K(+) currents when compared to oocytes expressing K(v1.5) only. We here present the first evidence on a biological relevance of FHL1C
LDB1 (LIM domain binding 1)
No full textReview on LDB1 (LIM domain binding 1), with data on DNA, on the protein encoded, and where the gene is implicated
Coefficient of correlation (variance explained) for the relationships between <i>t</i><sub><i>lim</i></sub>, <i>W’</i> and CP.
No full text<p>Coefficient of correlation (variance explained) for the relationships between <i>t</i><sub><i>lim</i></sub>, <i>W’</i> and CP.</p
Using broiler sound frequency to model weight
No full textChicken weight provides information about growth and feed conversion in order to identify deviations from the expected homogeneous growth trend of the birds. Precision Livestock Farming (PLF) can support the farmer through the use of sensors, cameras and microphones. Previous studies showed a significant correlation (p<0.001) between the frequency of vocalisation and the age and weight of the broiler. In this study, recordings were made in an automated, non-invasive way through the entire life of the birds, to evaluate the frequency variation of the sounds emitted during production cycles. In total, sound data collected during 8 production cycles (in an intensive broiler farm – 30,000 birds reared per round) were analysed. Sound data were manually and automatically compared with the weight of the birds automatically measured. Sound analysis was performed based on the amplitude and frequency of the sound signal in audio files recorded at farm level. The aim of this study was to sample automatically broiler vocalisations under normal farm conditions, to identify and model the relation between animal sounds and growth trend, and develop a tool to automatically detect the growth level of the animals based on the frequency of the vocalisation. The model used to predict the weight as a function of the Peak Frequency (PF) confirmed that the animal weight could be predicted by the frequency analysis of the sounds emitted at farm level although a more accurate editing of the audio file is necessary
Goniozus mesolevis Lim, sp. nov.
No full textGoniozus mesolevis Lim, sp. nov. (Figs 25–32) Type materials. Holotype. KOREA: JN: Ƥ, Pungsan, Dado, Naju, MT, 30.viii– 9.ix. 2005, S. B. Yu leg (KFRI). Paratypes. KOREA: Seoul: Ƥ, Cheongyangri, Dongdaemun, MT, 12–20.ix. 2005, D. P. Lyu leg. (KFRI). GG: Ƥ, Gwanak arboretum, Manan, Anyang, MT, 31 viii– 14.ix. 2007, J. O. Lim leg. (SNU). GW: Ƥ, Jinae, Dong, Chuncheon, MT, 2–10.vii. 2005, S. J. Jang leg. (KFRI); Ƥ, ditto, MT, 16–30.vi. 2006, S. J. Jang leg. (SNU); Ƥ, ditto, MT, 31.vii– 12.viii. 2007, S. J. Jang leg. (KFRI). CN: Ƥ, Donam, Banpo, Gongju, MT, 2–9.viii. 2005, Y. T. Kim leg. (KFRI); 2 Ƥ, ditto, MT, 23–29.vii. 2007, Y. T. Kim leg. (KFRI). GB: Ƥ, Namsa, Hyeongok, Kyeongju, MT, 11–18.viii. 2005, J. T. Kim leg. (SNU); 2 Ƥ, ditto, MT, 25.viii– 2.ix. 2005, J. T. Kim leg. (SNU); Ƥ, ditto, MT, 30.vi– 14.vii. 2005, J. T. Kim leg. (SNU). GN: 5 Ƥ, Dapcheon, Ibanseong, Jinju, MT, 12–26.ix. 2005, B. G. Ahn leg. (KFRI); 2 Ƥ, ditto, MT, 29.viii– 12.ix. 2005, B. G. Ahn leg. (KFRI); 2 Ƥ, ditto, MT, 11–28.vi. 2007, B. G. Ahn leg. (KFRI). JB: Ƥ, Majeong, Buk, Jeongeub, MT, 12–19.vii. 2005, J. W. Park leg. (KFRI); 4 Ƥ, ditto, MT, 19–26.vii. 2005, J. W. Park leg. (KFRI); 3 Ƥ, ditto, MT, 20–27.ix. 2005, J. W. Park leg. (KFRI); Ƥ, ditto, MT, 2–9.viii. 2005, J. W. Park leg. (KFRI); Ƥ, ditto, MT, 5–12.vii. 2005, J. W. Park leg. (KFRI). JN: Ƥ, Pungsan, Dado, Naju, MT, 25.vii– 8.viii. 2005, S. B. Yu leg. (KFRI); Ƥ, ditto, MT, 8–16.viii. 2005, S. B. Yu leg. (KFRI); 9 Ƥ, ditto, MT, 9–30.ix. 2005, S. B. Yu leg. (KFRI); Ƥ, ditto, MT, 25–31.viii. 2007, S. B. Yu leg. (KFRI). Diagnosis. This species species is similar to G. kusigematii Terayama, 1999 from Japan by having basal triangle area on propodeal disc absent, by longitudinal smooth area which get wide distally on propodeal disc, but can be easily distinguished from it by mandible black (yellow in G. k u s i g e m a t i i), by compound eye with short hairs (compound eye without hairs in G. kusigematii), by transverse carina on propodeal disc present only postero-lateral corner (transverse carina complete in G. k u s i g e m a t i i). Description. FEMALE (holotype). Body length 3.7 mm long. LFW 2.0 mm. Color. Head: mandible black, antenna yellow except flagellomere 5 to 11 and dorsal surface of basal half of scape castaenous. Mesosoma: black; fore wing subhyaline, veins pale castaneous; legs castaenous except coxa, tibia and tarsi yellow; tarsal claw dark castaenous. Metasoma: dark castaneous except distal surface of terga 2 to terminal pale castaenous. Head (Figs 26–28): 1.0 × as long as wide, coriaceous; lateral margin convex, posterior margin straight, postero-lateral corner forming round angle in dorsal view; lateral surface smooth and polished. Mandible with four minute teeth. Clypeus well-developed, frontal angle right; fronto-clypeal median longitudinal carina weakly developed, exceeding antennal socket. First antennal segment in ratio of 2.4: 1.1: 1.0: 1.2: 1.1 in length; from scape to flagellomere 3 and 11 2.2, 1.4, 1.4, 1.3, 1.5 and 2.0 × as long as wide, Frons and vertex coriaceous with sub-erect and relatively dense punctures, aparted from each other by 1.0–2.0 × as wide as their maximum diameter. WF 1.3 × LE, WF 0.7 × WH. Compound eye 0.35 mm long with short erect hairs. LE 1.6 × OOL, WF 1.9 × WOT. Frontal angle of ocellar triangle obtuse, POL 2.3 × AOL, OOL 0.9 × WOT. Vertex coriaceous with four long hairs on occipital margin. Mesosoma (Figs 29–31): Pronotum coriaceous, 0.6 × as long as wide with sparse hairs, antero-lateral corner obtuse. Mesoscutum coriaceous; notauli absent; parapsidal furrows thin and anteriorly divergent. Scutellum polish and coriaceous with sparse small punctures; scutellar pit elliptical, oblique and connected by 3.8 × as wide as their maximum diameter. Propodeal disc 0.5 × as long as wide, lateral carina complete, transverse carina present only postero-lateral corner; disc coriaceous except median longitudinal smooth surface, distally broaden in dorsal view; declivity coriaceous with complete marginal carina; lateral surface coriaceous. Fore wing with hairs and closed areolet; radial vein roundly curved; pterostigma 0.18 mm long; metacarpo absent. Metasoma (Fig. 32): Tergite 1 smooth and polished without fine punctures and microreticulation. Terga 2–4 smooth and polished with very fine and few punctures and sparse hairs on lateral surface. Terga 5 to terminal with sparse hairs on distal surface. MALE. Unknown. Distribution. Korea (CN, GB, GG, GN, GW, JB, JN, Seoul).Published as part of Lim, Jongok & Lee, Seunghwan, 2012, Review of Goniozus Förster, 1856 (Hymenoptera: Bethylidae) of Korea, with descriptions of two new species, pp. 43-57 in Zootaxa 3414 on pages 51-53, DOI: 10.5281/zenodo.21079
Functional diversity of LIM proteins amino-terminal activation domains in the oncogenic proteins RBTN1 and RBTN2
No full textThe RBTN1 and RBTN2 genes are activated by distinct translocations involving chromosome 11 in some T cell acute leukaemias. The RBTN proteins belong to the LIM family which comprises proteins with one, two or three cysteine-rich LIM domains, sometimes together with homeodomains or protein kinase domains. The RBTN1 and RBTN2 proteins comprise only tandem LIM domains. We report that RBTN1 and RBTN2 proteins are capable of supporting transcriptional transactivation of specific reporter genes in transfection assays. The results, using intact proteins or fusions with the homeodomain of the heterologous protein Isl-1, show that this transcriptional activation ability resides in the NH2-terminal parts of both proteins. The use of yeast assays with RBTN2 shows that RBTN2 forms homodimers and that the NH2-terminal 27 amino acids are sufficient to facilitate transcriptional transactivation. These data expand the functional diversity of the LIM-domain protein family and they augment the previously defined relationship between chromosomal translocations and transcriptional activation
Functional diversity of LIM proteins : amino-terminal activation domains in the oncogenic proteins RBTN1 and RBTN2
No full textThe RBTN1 and RBTN2 genes are activated by distinct translocations involving chromosome 11 in some T cell acute leukaemias. The RBTN proteins belong to the LIM family which comprises proteins with one, two or three cysteine-rich LIM domains, sometimes together with homeodomains or protein kinase domains. The RBTN1 and RBTN2 proteins comprise only tandem LIM domains. We report that RBTN1 and RBTN2 proteins are capable of supporting transcriptional transactivation of specific reporter genes in transfection assays. The results, using intact proteins or fusions with the homeodomain of the heterologous protein Isl-1, show that this transcriptional activation ability resides in the NH2-terminal parts of both proteins. The use of yeast assays with RBTN2 shows that RBTN2 forms homodimers and that the NH2-terminal 27 amino acids are sufficient to facilitate transcriptional transactivation. These data expand the functional diversity of the LIM-domain protein family and they augment the previously defined relationship between chromosomal translocations and transcriptional activation
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