267 research outputs found
The role of phosphorylation of ISGF3 components in the regulation of ISG expression and viral protection
No full textWydział BiologiiInterferony pełnią kluczową rolę we wzbudzaniu przeciwwirusowej odpowiedzi immunologicznej. W swojej klasycznej formie odpowiedź zależna od IFNα jest oparta na kompleksie ISGF3, składającym się z ufosforylowanych białek STAT1 i STAT2 oraz IRF9. Oprócz kanonicznej sygnalizacji w zapewnieniu ochrony przeciw wirusom zaangażowane są również inne kompleksy w tym U-ISGF3 (składający się z niefosforylowanych form STAT1 i STAT2 oraz IRF9) czy STAT2/IRF9 w swojej nie- lub ufosforylowanej formie. Stosując techniki molekularne, w tym także całogenomowe takie jak RNA- i ChIP-Seq, oraz przy użyciu JAK Inhibitor I, wyjaśniliśmy rolę jaką w odpowiedzi konstytutywnej, a także zależnej od interferonu i czasu, odgrywa proces fosforylacji. Zbadaliśmy dwa rodzaje linii komórkowych: typu dzikiego oraz pozbawione STAT1 i na tej podstawie wyciągnęliśmy wnioski dotyczące zależności ochrony przeciwwirusowej od ilości białek STAT1, STAT2 i IRF9 w komórce. Ponadto, eksperymenty porównawcze na komórkach ze stabilną nadprodukcją STAT1, STAT2 i IRF9 wykazały potencjalną rolę U-ISGF3 (i prawdopodobnie też U-STAT2/IRF9) w regulacji konstytutywnej oraz indukowanej IFNα przedłużonej ekspresji ISG i ochronie przeciw wirusom. To sugeruje, że musi zostać przekroczony pewien minimalny poziom białek STAT1, STAT2 i IRF9 w komórce (a co za tym idzie kompleksów U-ISGF3 i/lub U-STAT2/IRF9), aby doszło do zapoczątkowania ekspresji ISG. W konsekwencji, wraz z klasycznym kompleksem ISGF3, także U-ISGF3 i U-STAT2/IRF9 mogą odgrywać zasadniczą rolę w zależnej i niezależnej od IFNα transkrypcji ISG oraz w zwalczaniu infekcji wirusowych.Interferons play crucial role in the triggering of antiviral response, that in its most classical form is based on STAT1 and STAT2 that become phosphorylated and with IRF9 form ISGF3. Besides canonical ISGF3-dependent signalling, also other complexes function and may cooperate to provide effective viral protection. The examples are U-ISGF3 (that consists of unphosphorylated STAT1 and STAT2 together with IRF9) and STAT2/IRF9 in both unphosphorylated or phosphorylated form. Using different molecular techniques, among them whole-genome approaches, such as RNA-Seq and ChIP-Seq, in combination with JAK Inhibitor I, we provide further evidence on the role of phosphorylation in both, basal as well as IFN- and -time dependent transcriptional responses under wild-type and STAT1-deficiency conditions, we as well considered the dependence of viral protection on the amount of the native STAT1, STAT2 and IRF9. Additionally, comparative experiments in cells overexpressing STAT1, STAT2 and IRF9, revealed a potential role of U-ISFG3, and possibly U-STAT2/IRF9, in the regulation of constitutive and long-term IFNα-treated ISG expression and viral protection. This strongly suggests that a certain threshold of STAT1, STAT2 and IRF9 expression and levels of U-ISGF3 (and/or U-STAT2/IRF9) has to be reached to be able to trigger ISG transcription. As a consequence, together with classical ISGF3, U-ISGF3 and U-STAT2/IRF9, could be instrumental in IFN-dependent and independent ISG transcription and in combatting viral infection."Passport to the future - Interdisciplinary doctoral studies at the Faculty of Biology UAM" POWR.03.02.00-00-I006/17; PRELUDIUM research grant UMO-2016/21/N/NZ2/01720: The cooperation/role of unphosphorylated and hosphorylated ISGF3 components in the regulation of ISG expression and viral protection Project leader: MSc Hanna Janina Nowicka; OPUS research grant 2012-07-B-NZ1-02710: STAT1 and IRF8-mediated signal integration between IFNg and TLRs: a pathophysiological role in vascular disease Project leader: Prof. Johannes A.R. Bluyssen; OPUS research grant 2013/11/B/NZ2/02569: Genome-wide characterization of type I IFN-induced transcriptomes and identification of novel ISGs and their antiviral functions Project leader: Prof. Johannes A.R. Bluysse
Po kładce nad urwiskiem, przymrużając oko. O tłumaczeniu poezji humorystycznej na przykładzie Rymowanek Szymborskiej
No full textThe article presents a practical-theoretical reflection on translating humoristic poetry on the basis of the Author’s experience as a translator of Wisława Szymborska’s Rymowanki dla dużych dzieci. Since humor is the dominant function of the text, and laugh is the expected reaction, functional equivalence - rather than semantic equivalence - is here to be pursued. The goal of any translator of humoristic texts is to produce an equivalent effect on the reader. In the case of Szymborska’s Rymowanki, the task is made more difficult by the poetic form, which plays a primary role in generating the comic effect. The Author notices that the three categories of joke – universal, cultural and linguistic - proposed by Debra Raphaelson-West do not take into account such highly formalized utterings as humoristic verses, in which the form is not a mere decoration aiming at potentiating the aesthetic effect, but definitely contributes to generate the humoristic effect. The article shows how the mechanisms of laugh/smile proper of these poetic texts were reproduced in translation following some simple principles, and ends with the proposal to add to Raphaelson-West’s classification a fourth category: the poetic joke
Ten wiecznie obcy. Szkic o peregrynacjach Juliusza Słowackiego
No full textThe idyllic Krzemieniec, where the poet was bom, many of the greatest cities of the nineteenth century Europe and hated capital of France, finally - the courtesy visit to the king of Egypt and the recluse time in the Betcheshban monastery, hidden in the mountains of Lebanon ... Despite thousands of places that Juliusz Slowacki had visited rarely we think ofhim as a traveler. This "highly-qualified emigrant': even though he had spent most of his life in Paris, the capital of flaneurs, experienced the life of emigrant also in other countries. The oddity of poet was even expressing in the way he discovered new places, which, no matter its kind, never seemed to satisfy him for a long time. What was the nature ofhis traveling and self-perception as an eterna! "the other" and an uncanny stranger? What was the author of Podróż do Ziemi
Świętej escaping from?MARTA JUSTYNA NOWICKA - doktorantka Filologicznych Studiów Doktoranckich na Uniwersytecie Gdańskim. Absolwentka gdańskiej polonistyki, w życiu zawodowym związana z kreatywnym światem gier planszowych, w życiu naukowym z magicznym światem polskiego romantyzmu (za którym tęskni w godzinach pracy). W swoich tekstach porusza tematykę związaną z płcią, emocjami i odzyskiwaniem znaczeń. Pracuje nad dysertacją ukazującą postać Juliusza Słowackiego z perspektywy queer studies. W kręgu jej zainteresowań znajduje się nowy brutalizm, feminizm trzeciej fali oraz nieśmiałe próby obcowania z psychoanalizą jako metodą interpretacji literatury.UNIWERSYTET GDAŃSKI12713
Szacowanie prędkości pojazdów na podstawie obrazu z kamery = Estimating vehicle speed based on image from camera
No full textRajs Arkadiusz, Goździewska-Nowicka Agnieszka, Banaszak-Piechowska Agnieszka, Gospodarczyk Jacek. Szacowanie prędkości pojazdów na podstawie obrazu z kamery = Estimating vehicle speed based on image from camera. Journal of Education, Health and Sport. 2016;6(4):122-126. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.49880
http://ojs.ukw.edu.pl/index.php/johs/article/view/3456
The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015).
755 Journal of Education, Health and Sport eISSN 2391-8306 7
© The Author (s) 2016;
This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland
Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,
provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License
(http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.
This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial
use, distribution and reproduction in any medium, provided the work is properly cited.
The authors declare that there is no conflict of interests regarding the publication of this paper.
Received: 20.03.2016. Revised 17.04.2016. Accepted: 17.04.2016.
SZACOWANIE PRĘDKOŚCI POJAZDÓW NA PODSTAWIE OBRAZU Z KAMERY
ESTIMATING VEHICLE SPEED BASED ON IMAGE FROM CAMERA
dr inż. Arkadiusz Rajs
Uniwersytet Technologiczno-Przyrodniczy im. Jana i Jędrzeja Śniadeckich w Bydgoszczy, Wydział Telekomunikacji, Informatyki i Elektrotechniki,
Zakład Teletransmisji, Al. Prof. S. Kaliskiego 7, 85-790 Bydgoszcz, [email protected]
dr Agnieszka Goździewska-Nowicka
Uniwersytet Technologiczno-Przyrodniczy im. Jana i Jędrzeja Śniadeckich w Bydgoszczy, Wydział Zarządzania, Katedra Organizacji i Zarządzania,
ul. Fordońska 430, 85-790 Bydgoszcz, [email protected]
dr Agnieszka Banaszak-Piechowska
Uniwersytet Kazimierza Wielkiego w Bydgoszczy
Instytut Fizyki, ul. Weyssenhoffa 11, 85-090 Bydgoszcz, [email protected]
dr inż. Jacek Gospodarczyk
Wyższa Szkoła Gospodarki w Bydgoszczy
Instytut Informatyki i Mechatroniki
Garbary 2,85-229 Bydgoszcz, [email protected]
Streszczenie
Praca prezentuje sposób oszacowania prędkości poruszających się pojazdów.
Opisano metodę opartą na analizie obrazu oraz przedstawiono wyniki pomiarów.
Abstract
This Work presents a way to estimate the speed of the moving vehicles.
Describes a method based on image analysis, and shows the results of the measurements.
Słowa kluczowe: analiza obrazu, prędkość pojazdów, systemy wizyjne.
Key words: image analysis, machine vision systems, vehicle speed
Composite morphological structural element in the edge detecting
No full textRajs Arkadiusz, Aleksiewicz Mariusz, Goździewska-Nowicka Agnieszka, Parczyk Krystyna. Composite morphological structural element in the edge detecting. Journal of Education, Health and Sport. 2016;6(11):299-304. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.167138
http://ojs.ukw.edu.pl/index.php/johs/article/view/3996
The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015).
755 Journal of Education, Health and Sport eISSN 2391-8306 7
© The Author (s) 2016;
This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland
Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,
provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License
(http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.
This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial
use, distribution and reproduction in any medium, provided the work is properly cited.
The authors declare that there is no conflict of interests regarding the publication of this paper.
Received: 01.11.2016. Revised 12.11.2016. Accepted: 16.11.2016.
COMPOSITE MORPHOLOGICAL STRUCTURAL ELEMENT IN THE EDGE DETECTING
dr inż. Arkadiusz Rajs
Institute of Telecommunications and Computer Science, University of Science and Technology,
Al. Prof. S. Kaliskiego 7, 85-789 Bydgoszcz, [email protected]
dr inż. Mariusz Aleksiewicz
Institute of Telecommunications and Computer Science, University of Science and Technology,
Al. Prof. S. Kaliskiego 7, 85-789 Bydgoszcz,, [email protected]
dr Agnieszka Goździewska-Nowicka
University of Science and Technology, Faculty of Management,
ul. Fordońska 430, 85-790 Bydgoszcz, [email protected]
dr Krystyna Parczyk
WSB University in Toruń, ul. Młodzieżowa 31a, 87-100 Toruń, [email protected]
Summary. Edge detection is an important step in image analysis. Using mathematical morphology and individual structural elements can detect certain types of edges. But much remains undetected. This paper proposes edge detection algorithm based on the structural element made up of many individual structure elements. The experimental results are showing.
Key words: mathematical morphology, edge detectio
Drowning in the blue, a vision of romantic love in Juliusz Słowacki's and Zygmunt Krasiński's letters
No full textThe epistolary consciousness of the romantics was exceeding the boundaries of the applied letter, romantic improvisation or the existential experiment - it was becoming an attempt to create a new language and new, extremely emotional form of artistic form of expression. The concept of the romantic open text adopted the idea of putting a special emphasis on the interactions between the letters (especially the poetic ones) - on their complementarity and permeation. What was the way in which Juliusz Słowacki and Zygmunt Krasiński were defining themselves and their relation as they were corresponding with each other during 9 years of
turbulent relationship? What do the letters say about those two authors and their real feelings? How do the letters of philomaths differ from the correspondence of poets who were "drowning in the blue"? In this presentation the author will try to refer to these and other elements of romantic language, to an emotion as a component of life-writing, and also to the similarities and differences which can be found in letters of Polish romantics. The paper is the part of PhD thesis, dedicated to non-heteronormative sensitivity of Juliusz Słowacki.MARTA JUSTYNA NOWICKA - doktorantka Filologicznych Studiów Doktoranckich na Uniwersytecie Gdańskim. Absolwentka gdańskiej polonistyki, w życiu zawodowym związana z kreatywnym światem gier planszowych, w życiu naukowym z magicznym światem polskiego romantyzmu (za którym tęskni w godzinach pracy). W swoich tekstach porusza tematykę związaną z płcią, emocjami i odzyskiwaniem znaczeń. Pracuje nad dysertacją ukazującą postać Juliusza Słowackiego z perspektywy queer studies. W kręgu jej zainteresowań znajduje się nowy brutalizm, feminizm trzeciej fali oraz nieśmiałe próby obcowania z psychoanalizą jako metodą interpretacji literatury.253
Neuroimaging methods for analysing connectivity in the presence of white matter lesions
No full textWhite Matter Hyperintensities (WMHs) are often observed in the MRI scans of the ageing brain. Previous studies show that the WMH load correlates with cognitive decline, as well as with an increased risk of stroke and dementia. Most of the studies use a global WMH load across the whole brain as the only metric. This thesis proposes new insight into such data by introducing a methodology to analyse the WMH impact on the structural and functional brain connectivity in a localised manner, using white matter tracts. The thesis also supports other studies by exploring the potential impact of WMHs presence on tractography modelling, as well as adapts the method to Multiple Sclerosis (MS) lesion data.
In the first part of the thesis, we presented and evaluated two possible measures of the WMHs impact on the WM tracts from the structural perspective. We showed that despite the different distributions of the two measures, both show a similar relationship with the functional connectivity measure. We explored several possible options for quantifying the resting state functional connectivity between the endpoints of the WM tract in the presence of WMHs. We showed that the choice of connectivity measure (full or partial correlation), as well as gray matter parcellation, made a substantial difference in the results. We also observed variability in the results among the tracts and compared our findings to the results from the literature.
The majority of this work depends on the correct definition of the white matter tracts. Therefore, the second part of the thesis focuses on evaluating the impact of the presence of WMHs on tractography modelling. Despite changes in the microstructural parameters within the WMHs and their proximity, we found no meaningful alterations in the shape of the WM tracts in the presence of WMHs on the tract. Finally, we explored the possibility to apply some of this methodology to data from patients with Multiple Sclerosis, which is the most common cause of neurological disability in young adults. MS lesions have different aetiology to the WMHs but may have a similar appearance. We showed that directly applying the method between those two conditions may not be possible, and we proposed an alternative approach, suited to the MS data
Organizacje polonijne i tożsamość narodowa. Polacy w Bośni i Hercegowinie (BiH)
No full textThe article is based on anthropological fieldwork basing on participant observation andinterviews conducted in 2014 during Program „Sigma” Erazmus Mundus 2 in Tuzla,Lukavac, Sarayevoand Mostar. The interviewees were the members of Polish minority inBosnia and Herzegovina engaged in Polish cultural organisations. The study aimed atunderstanding various types of bonds with Poland as a state or the culture and even familyhistories, as presented in interviews and behaviours. The author poses the question: whyintence development of Polish organisations flourished in the period of war in 1991-1995,inspite of the fact that Polish emigration to Bosnia started in the second half of 19th century. Presently the organizations are fading and even dissapearing. The author traces manifestations of Polish identity and national ties in Bosnia and presents typology, linking particular types of Polish identity withtime and character ofemigration.The article is based on anthropological fieldwork basing on participant observation andinterviews conducted in 2014 during Program „Sigma” Erazmus Mundus 2 in Tuzla,Lukavac, Sarayevoand Mostar. The interviewees were the members of Polish minority inBosnia and Herzegovina engaged in Polish cultural organisations. The study aimed atunderstanding various types of bonds with Poland as a state or the culture and even familyhistories, as presented in interviews and behaviours. The author poses the question: whyintence development of Polish organisations flourished in the period of war in 1991-1995,inspite of the fact that Polish emigration to Bosnia started in the second half of 19th century. Presently the organizations are fading and even dissapearing. The author traces manifestations of Polish identity and national ties in Bosnia and presents typology, linking particular types of Polish identity with time and character of emigration
Cloud Computing in Sustainable Mobility
No full textAbstractSustainable mobility takes into account a broader approach than sustainable transport as it is a concept integrating realization of the needs of stakeholders by using remote access to the properties of desired goods and services. It realizes the need to minimize the negative impact on the environment. Sustainable mobility remains one of the most important subject and challenge influencing European competitiveness. Transport activities apply to micro and macroeconomic issues, moving between and across various sectors, is still the highest of global logistics costs. By using cloud computing model in mobility and transportation management systems managers can gain reduction of the total cost of provided services for residents; more agility, flexibility, and elasticity; quick and cost-efficient reaction to less-predictable events and changing customer (or other stakeholders) requirements; risk reduction, globally accessible services, easy and fast implementation and strong support for sustainable development. The use of cloud computing in sustainable mobility is than mobility of people and cargos with respect to energy, environment, safety and security as well as socio-economic issues by giving access to real-time data, elasticized, fast reaction to the events and their negative consequences in available, efficient and affordable manner to all interested stakeholders. Cloud computing has already been noticed by European Union and exists in different acts and regulations’ recommendations, but it is definitely insufficiently analyzed and, most important, implemented in the mobility and transportation aspects
Antropologist in Relation to Himself and Others. Some Ethical Problems in Doing Antropology
No full textArtykuł skupia się na analizie doświadczeń terenowych autorki, koncentrując uwagę na komplikacjach emocjonalnych i aksjologicznych wynikających z długotrwałej współpracy z badanymi, nawiązywaniem więzi osobistych i rodzącym się zaangażowaniem badacza w sprawy badanego społeczeństwa. Autorka sięga do antropologicznej refleksji podejmującej tematy etyczne oraz twórczości
Marii Ossowskiej, znajdując w niej fundamentalne inspiracje.The article concentrates on the analysis of the author’s field experience. It focuses on emotional and axiological dilemmas
and complexities resulting from long-lasting cooperation with researched group. The effects of the process are on one side personal
bonds between researcher and researchees (sometimes deep friendship and affection), and on the other side his/her engagement
in studied society problems. The author uses not only literature about anthropological reflection on ethical issues in fieldwork, but
also implies Maria Ossowska’s works, furnishing rich inspirations
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