849 research outputs found
Intracellular clusterin negatively regulates ovarian chemoresistance: compromised expression sensitizes ovarian cancer cells to paclitaxel
No full textUnderstanding the molecular events that lead to
paclitaxel (TX) resistance is necessary to identify effective
means to prevent chemoresistance. Previously, results from
our lab revealed that secretory clusterin (CLU) form
positively mediates TX response in ovarian cancer cells.
Thus, we had interest to study the role of another nonsecreted
form (intracellular clusterin (i-CLU)) in chemoresponse.
Here, we provide evidences that i-CLU form
localizes mainly in the nucleus and differentially expressed
in the TX-responsive KF cells, versus TX-resistant, KF-TX,
ovarian cancer cells and negatively regulate cellular chemoresponse.
I-CLU was cloned, by deleting the secretionleading
signaling peptide from full-length CLU cDNA, and
transiently over-expressed in OVK-18 cells. Forced expression
of truncated i-CLU was mainly detectable in the nuclei
and significantly reduced cellular growth, accumulating
cells in G1 phase which finally died through apoptosis.
Importantly, compromised expression of i-CLU under an
inducible promoter was tolerated and did not induce
apoptosis but sensitized ovarian cancer cells to TX. We
then demonstrated that this sensitization mechanism was
cell cycle independent and relied on i-CLU/Ku70 binding
probably due to controlling the free amount of Ku70
available for DNA repair in the nucleus. Results from CLU
immunehistochemistry in ovarian tumor tissues verified the
retardation of nuclear CLU staining in the recurrent tumor
even though their primary counterparts showed nuclear
CLU staining. Thus, the controversial data on CLU
function in chemo-response/resistance may be explained
by a shift in the pattern of CLU expression and intracellular
localization as well when tumor acquires chemoresistance
LUP877258 Supplemental Material - Supplemental material for Pregnancy outcomes in women with rheumatic diseases: a real-world observational study in Japan
No full textSupplemental material, LUP877258 Supplemental Material for Pregnancy outcomes in women with rheumatic diseases: a real-world observational study in Japan by E Sugawara, M Kato, Y Fujieda, K Oku, T Bohgaki, S Yasuda, T Umazume, M Morikawa, H Watari and T Atsumi in Lupus</p
Watari, Joe H.
No full textPassport photo of man. Negative scan.In 1922, Kinso Ninomiya opened the Ninomiya Photography Studio in Little Tokyo, Los Angeles. Due to Executive Order 9066 in 1942, the studio was forced to close but was reopened by Kinso and his son, Elwin Ichiro, in 1949. The studio operated in Little Tokyo until its final closing in 1986. The Ninomiya Studio Collection captures slices of Japanese American life in Los Angeles from the 1950s through the 1980s. The collection contains formal portraiture and candid photography in black and white and color as well as commercial photography for local businesses and reproductions of older photographs. The negatives come in a variety of sizes, including 8 x 10 inch negatives and panoramic negatives on Cirkut film. Each negative scanned has been selected out of multiple negatives and prints from a set. The title of the negative scan reflects the purchaser’s name
福島第一原発事故,2017年,福島市渡利・小倉寺の里山の汚染の実態
No full text2018-03-31In May of 2017, the author investigated the air radiation dose (1m above the ground) at the village forest in Watari and Oguraji areas. The range of the radiation dose was 0.15~1.77 μSv/h and its average was 0.56 μSv/h. In August of 2017, the author investigated the air radiation dose (1m above the ground) at the village forest in Watari area. The range of the radiation dose was 0.09~1.16 μSv/h and its average was 0.35 μSv/h. Decontamination work of this area started in 2012 and has been ended at the beginning of the investigation. However, the radiation dose rate in this area after the decontamination was still higher than the target value of 0.23 μSv/h tentatively proposed by Ministry of the Environment.2017年5月に福島市の渡利・小倉寺の里山において空間放射線率（高さ1m）を調査した．測定点は1039地点，空間放射線量率（地上1m）は0.15～1.77μSv/h，平均は0.56μSv/hであった．また，2017年8月，福島市渡利の弁天山においても同様の調査をした．測定点は830点，空間線量率(地上1m)は0.09～1.16μSv/h，平均は0.35μSv/hであった．公的な除染作業は，本調査時には終了している．しかし，空間放射線量率（地上1m）は除染の目標値0.23μSv/h以下には達していない．また，除染物を詰めた袋の放置，除染土の廃棄も見られた．departmental bulletin pape
Dynamic Programming for the Subset Sum Problem
No full textThe subset sum problem is a basic problem in the field of theoretical computer science, especially in the complexity theory [3]. The input is a sequence of positive integers and a target positive integer. The task is to determine if there exists a subsequence of the input sequence with sum equal to the target integer. It is known that the problem is NP-hard [2] and can be solved by dynamic programming in pseudo-polynomial time [1]. In this article we formalize the recurrence relation of the dynamic programming.This work was supported by JSPS KAKENHI Grant Numbers JP16K00033, JP17K00013 and JP17K00183.Hiroshi Fujiwara - Shinshu University, Nagano, JapanHokuto Watari - Nagano Electronics Industrial Co., Ltd., Chikuma, JapanHiroaki Yamamoto - Shinshu University, Nagano, JapanMichael R. Garey and David S. Johnson. Computers and Intractability: A Guide to the Theory of NP-Completeness. W. H. Freeman & Co., New York, NY, USA, 1979. ISBN 0716710447.Richard M. Karp. Reducibility among combinatorial problems. In Miller et al. [3], pages 85–103. ISBN 978-1-4684-2001-2. doi:10.1007/978-1-4684-2001-2_9.Raymond E. Miller, James W. Thatcher, and Jean D. Bohlinger, editors. Complexity of Computer Computations, 1972. Springer US. ISBN 978-1-4684-2001-2. doi:10.1007/978-1-4684-2001-2_9.Wojciech A. Trybulec. Non-contiguous substrings and one-to-one finite sequences. Formalized Mathematics, 1(3):569–573, 1990.899
A Questionnaire Study of Leadership in General Medicine: General Physicians in Japan are Facing Challenges in Education and Research [Response to Letter]
No full textRisa Hirata,1 Masaki Tago,1 Hiromizu Takahashi,2 Takashi Watari,3 Kiyoshi Shikino,4,5 Yosuke Sasaki,6 Taro Shimizu7 1Department of General Medicine, Saga University Hospital, Saga, Japan; 2Department of General Medicine, Faculty of Medicine, Juntendo University, Tokyo, Japan; 3Integrated Clinical Education Center, Kyoto University Hospital, Kyoto, Japan; 4Department of General Medicine, Chiba University Hospital, Chiba, Japan; 5Department of Community-Oriented Medical Education, Chiba University Graduate School of Medicine, Chiba, Japan; 6Department of General Medicine and Emergency Care, Toho University School of Medicine, Tokyo, Japan; 7Department of Diagnostic and Generalist Medicine, Dokkyo Medical University, Tochigi, JapanCorrespondence: Masaki Tago, Department of General Medicine, Saga University Hospital, 5-1-1 Nabeshima, Saga, 849-8501, Japan, Tel +81 952 34 3238, Fax +81 952 34 2029, Email [email protected]
Precise Classification of Generalists is Necessary to Establish Evidence in General Medicine [Response to Letter]
No full textRisa Hirata,1 Masaki Tago,1 Kiyoshi Shikino,2,3 Takashi Watari,4 Hiromizu Takahashi,5 Yosuke Sasaki,6 Taro Shimizu7 1Department of General Medicine, Saga University Hospital, Saga, Japan; 2Department of General Medicine, Chiba University Hospital, Chiba, Japan; 3Department of Community-Oriented Medical Education, Chiba University Graduate School of Medicine, Chiba, Japan; 4Integrated Clinical Education Center, Kyoto University Hospital, Kyoto, Japan; 5Department of General Medicine, Faculty of Medicine, Juntendo University, Tokyo, Japan; 6Department of General Medicine and Emergency Care, Toho University School of Medicine, Tokyo, Japan; 7Department of Diagnostic and Generalist Medicine, Dokkyo Medical University, Tochigi, JapanCorrespondence: Masaki Tago, Department of General Medicine, Saga University Hospital, 5-1-1 Nabeshima, Saga, 849-8501, Japan, Tel +81 952 34 3238, Fax +81 952 34 2029, Email [email protected]
Vector-like pairs and Brill–Noether theory
No full textAbstractHow likely is it that there are particles in a vector-like pair of representations in low-energy spectrum, when neither symmetry nor anomaly consideration motivates their presence? We address this question in the context of supersymmetric and geometric phase compactification of F-theory and Heterotic dual. Quantisation of the number of generations (or net chiralities in more general term) is also discussed along the way. Self-dual nature of the fourth cohomology of Calabi–Yau fourfolds is essential for the latter issue, while we employ Brill–Noether theory to set upper bounds on the number ℓ of vector-like pairs of chiral multiplets in the SU(5)GUT (5+5¯) representations. For typical topological choices of geometry for F-theory compactification for SU(5) unification, the range of 0≤ℓ≲4 for perturbative unification is not in immediate conflict with what is already understood about F-theory compactification at this moment
Construction of the global potential energy surface of the [H,S,F] system
No full textEste projeto tem dois objetivos. Primeiramente estudou-se a aplicabilidade dos splines tricúbicos para a construção de superfícies de energia potencial globais. Um dos obstáculos que este método tem de superar e a escolha de um sistema de coordenadas apropriado, que minimize a influência de pontos não físicos. Para isto, propôs-se o uso do sistema de coordenadas de Pekeris, nunca usado para este fim. Este procedimento foi realizado para três sistemas químicos bem descritos na literatura, [Cl,H2], [F,H,D] e [H,O,Cl], cujas superfícies de energia potencial e propriedades das reações foram usadas como referência. Com base nestes modelos, aplicamos o método proposto variando-se a quantidade e a disposição dos nós das interpolações, a fim de verificar sua influência na qualidade das superfícies interpoladas. Os resultados mostram que as superfícies construídas por este método reproduzem muito bem os cálculos de dinâmica química, tanto por métodos quânticos quanto por métodos clássicos. Para isto, os nós da interpolação devem cobrir as regiões mais importantes da superfície de energia potencial e os valores mais baixos das coordenadas de Pekeris devem ser priorizados. O segundo objetivo consiste na aplicação deste procedimento na construção da superfície de energia potencial [H,S,F]. Com esta superfície, diversas características deste sistema foram analisadas, tais como geometrias dos pontos estacionários, energias relativas e frequências vibracionais. Os valores obtidos estão de acordo com os dados descritos na literatura. A superfície construída também foi usada para a realização de cálculos de dinâmica para a reação F+HS → S+FH. Observamos a existência de dois tipos de mecanismos, um com a formação de um intermediário de longa duração e outro com a abstração direta do átomo de hidrogênio.This project has two goals. First, we studied the applicability of the tricubic splines to construct global potential energy surfaces. One of the diculties this approach has to overcome is the choice of an appropriate coordinate system that minimises the in uence of non-physical points. For such, we proposed the use of the Pekeris coordinate system, never employed for this purpose. This procedure was carried out for three well described systems, [Cl,H2], [F,H,D] and [H,O,Cl], whose potential energy surfaces and reaction properties were taken as references. Based on these models, we applied the proposed method varying the amount and arrangement of the interpolation knots, to verify their influence on the quality of the interpolated surfaces. The results showed that surfaces constructed by this approach reproduce very well the chemical dynamics calculations, both for the quantum as well as for the classical methods, provided that the interpolation knots cover the most important regions of the potential energy surfaces, and the lower values of the Pekeris coordinates are prioritised. The second goal was the application of this procedure to the construction of the [H,S,F] potential energy surface. With this surface, several characteristics of this system were analysed, such as the geometry of the stationary points, relative energies and vibrational frequencies. The values obtained are in agreement with the data described in the literature. The constructed surface was also used for quantum dynamics calculations on the reaction F + HS → S + FH. We observed two kinds of mechanisms, one of them with the formation of a long-living intermediate and the other with the direct abstraction of the hydrogen atom
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