77 research outputs found
Antoni B. Stepien, O metodzie teorii poznania. Rozwazania wstepne [Summary : On the method of the theory of knowledge. Introductory considerations]
No full textKalinowski Georges. Antoni B. Stepien, O metodzie teorii poznania. Rozwazania wstepne [Summary : On the method of the theory of knowledge. Introductory considerations]. In: Revue Philosophique de Louvain. Troisième série, tome 65, n°87, 1967. pp. 391-393
Antoni B. Stepien, O metodzie teorii poznania. Rozwazania wstepne [Summary : On the method of the theory of knowledge. Introductory considerations]
No full textKalinowski Georges. Antoni B. Stepien, O metodzie teorii poznania. Rozwazania wstepne [Summary : On the method of the theory of knowledge. Introductory considerations]. In: Revue Philosophique de Louvain. Troisième série, tome 65, n°87, 1967. pp. 391-393
2023 Hollister Lecture
No full textMr. Stepien graduated from Elmhurst College in 1965 and began his career as a middle school social studies teacher. In 1978, he became a program developer and senior author for the K-7 social studies series for the Scott, Foresman Company. When IMSA opened in 1985, he was tapped to plan curriculum, hire faculty, and help with facility design. Joining its faculty, he taught at IMSA for ten years. In 1994, Mr. Stepien became IMSA’s director of the Center for Problem-Based Learning, turning his full attention to research and writing about applications of PBL in elementary and secondary schools. He established the Consortium for Problem-Based Learning at Northern Illinois University in 1997 and became a national consultant in PBL for the Association for Supervision and Curriculum Development (ASCD). Mr. Stepien consulted with more than fifty schools every year, presented at numerous national meetings and conventions, and consulted with organizations such NASA, the Museum of Science and Industry (Chicago), Prime Time School Television, and The Wall Street Journal
Designing bioinformatic tools to model metabolic pathways and their regulation
No full textLa biologie des systèmes actuelle s’appuie sur des techniques d’analyse biologique à haut débit comme la transcriptomique ou la métabolomique. Cependant, ces techniques haut débit ont leurs limites et peuvent générer des erreurs. En croisant les résultats de différentes techniques d’analyse biologique, nous espérons pallier une partie de leurs limites. À cet effet, nous avons commencé à développer une plateforme de modélisation, MPSA (Metabolic Pathways Software Analyzer), permettant d’intégrer les données générées à des réseaux métaboliques. MPSA permet de représenter les graphes de voies métaboliques, d’effectuer des simulations basées sur la résolution de systèmes d’équations différentielles et d’étudier la structure des réseaux métaboliques par le calcul et la représentation des modes élémentaires. Nous avons développé différentes applications web permettant, d’une part, l’interprétation des résultats biologiques en utilisant des bases de données et, d’autre part, leur export vers MPSA. La base de données centrale de ce développement est myKegg, incluant l’ensemble des voies métaboliques humaines de la base de données publique KEGG ainsi qu’une base de synonymes construite elle aussi à partir de KEGG. Cette base permet d’identifier des voies métaboliques et de les importer dans MPSA. Une base de données de métabolomique, BioNMR, a aussi été construite spécifiquement pour organiser les résultats générés à partir de spectres de RMN. Une autre application web, GeneProm, a été développée pour l’analyse de promoteurs de gènes ou promotologie. Un protocole d’étude a été mis au point et testé sur un groupe de 4 gènes codant pour les isoformes 1 à 4 de la protéine ANT, transporteur mitochondrial d’ATP, chacune ayant un rôle et un profil d’expression spécifique dans la bioénergétique cellulaire. L’étude par promotologie de ces 4 gènes a permis d’identifier des éléments de régulation spécifiques dans leurs séquences promotrices et d’identifier des gènes potentiellement co-régulés. Ces gènes peuvent ensuite être exportés vers notre plateforme MPSA. L’ensemble de ce développement sera inclus au projet de plateforme intégrative de l’Unité de Nutrition Humaine de l’INRA.Current systems biology relies on high-throughput biological analysis techniques such as transcriptomics or metabolomics. However, these techniques may generate errors. By crossing results from different analysis techniques, we hope to avoid at least part of these limits. For that purpose, we started to develop a modeling platform, MPSA (Metabolic Pathways Software Analyzer). MPSA allows integrating biological data on metabolic pathways. MPSA also ensures the display of metabolic pathways graphs, the simulation of models based on ordinary differential equations systems solving and the study of network structures using elementary flux modes. We have developed several web applications allowing on the one hand to interpret biological results by using databases, and on the other hand to export these data to MPSA. The main database of this work is myKegg. It includes all human KEGG metabolic pathways and a list of synonyms for human KEGG entries. This base allows to identify metabolic pathways from a list of biological compounds and to import them in MPSA. Another database, BioNMR, has been developed to organize the data extracted from NMR spectra. Another web application named GeneProm has been developed to analyze gene promoters. A promotology protocol was developed and tested on a set of four genes coding for the four ANT (adenine nucleotide translocator) protein isoforms. Each ANT isoform has a specific expression profile and role in cell bioenergetics. The promotology study of these four genes led us to construct specific regulatory models from identified regulatory elements in their promoter sequence. Potentially co-regulated genes were deduced from these models. Then they can be exported to our MPSA platform. This whole development will be included in the project of Integrative Biology platform in the INRA Human Nutrition Unit
Unknown God, Known in His Activities: Incomprehensibility of God during the Trinitarian Controversy of the 4th Century
No full textUnknown God, Known in His Activities: Incomprehensibility of God during the Trinitarian Controversy of the 4th Century (European Studies in Theology, Philosophy and History of Religions) Tomasz Stepien (Author), Karolina Kochanczyk-Boninska (Author), 2018. What can man know about God? This question became one of the main problems during the 4th-century Trinitarian controversy, which is the focus of this book. Especially during the second phase of the conflict, the claims of Anomean Eunomius ..
Computational analysis of the transcriptional regulation of the adenine nucleotide translocator isoform 4 gene and its role in spermatozoid glycolytic metabolism
No full textInternational audienceComputational phylogenetic analysis coupled to promoter sequence alignment was used to understand mechanisms of transcriptional regulation and to identify potentially coregulated genes. Our strategy was validated on the human ANT4 gene which encodes the fourth isoform of the mitochondrial adenine nucleotide translocator specifically expressed during spermatogenesis. The movement of sperm flagella is driven mainly by ATP generated by glycolytic pathways, and the specific induction of the mitochondrial ANT4 protein presented an interesting puzzle. We analysed the sequences of the promoters, introns and exons of 30 mammalian ANT4 genes and constructed regulatory models. The whole human genome and promoter database were screened for genes that were potentially regulated by the generated models. 80% of the identified co-regulated genes encoded proteins with specific roles in spermatogenesis and with functions linked to male reproduction. Our in silico study enabled us to precise the specific role of the ANT4 isoform in spermatozoid bioenergetics
Altérations génétiques et métaboliques de la mitochondrie (pathologies et vieillissement)
No full textCLERMONT FD-BCIU-Santé (631132104) / SudocPARIS-BIUP (751062107) / SudocSudocFranceF
Accumulation of random mitochondrial DNA deletions and phenotypic consequences in aged human skeletal muscle
No full textInternational audienc
Computational identification of transcriptionally co-regulated genes, validation with the four ANT isoform genes
No full textÉvénement(s) lié(s) : - 37. FEBS Congress; Seville (ESP) - (2012-09-04 - 2012-09-09)International audienc
Quel rôle spécifique pour ANT2 dans une cellule cancéreuse ?
No full textLa translocase des nucléotides adényliques (ANT) réalise l’échange ATP/ADP entre le cytoplasme et la mitochondrie. Les isoformes ANT1 (musculaire) et ANT3 (ubiquitaire) exportent l’ATP produit par les phosphorylations oxydatives mitochondriales. L’isoforme ANT2 est spécifiquement exprimée dans les cellules en prolifération, dotées d’un métabolisme majoritairement glycolytique. ANT2 est ainsi associée à la dédifférenciation cellulaire, caractéristique majeure de la cancérogenèse. Son rôle serait d’importer dans la mitochondrie l’ATP produit par la glycolyse, énergie indispensable à différentes fonctions intramitochondriales, notamment au maintien du gradient de potentiel membranaire qui conditionne la survie et la prolifération cellulaires. Le mécanisme de régénération de ce gradient pourrait impliquer trois protéines majeures : l’hexokinase II, l’ANT2 et la partie F1 de l’ATP synthétase mitochondriale. Ainsi, l’ANT2, grâce à son rôle déterminant dans la croissance de la cellule tumorale, pourrait être choisie comme cible dans une stratégie anticancéreuse.In the mitochondrial internal membrane, the adenine nucleotide translocator (ANT) carries out the ATP/ADP exchange between cytoplasm and mitochondrial matrix. Three isoforms with different kinetic properties are encoded from three different genes in Human : the muscle specific ANT1 and the ubiquitary ANT3 isoforms export ATP produced by mitochondrial oxidative phosphorylation (OXPHOS). The ANT2 isoform is specifically expressed in proliferative cells with a predominant glycolytic metabolism and is associated with cellular undifferentiation which is a major characteristic in carcinogenesis. Its role would be to import into mitochondria ATP produced by the glycolysis, energy essential to several intramitochondrial functions, particularly to maintenance of the membrne potential (ΔΨm), conditioning cellular survival and proliferation. The mechanism of regeneration of this ΔΨm gradient would involve at least three major proteins : the hexokinase II isoform, the ANT2 isoform and the F1 part of the mitochondrial ATP synthase complex. Taking into account this major role of ANT2 in cell proliferation and the very low expression of this isoform in differentiated tissues, this protein or its transcript could be chosen as a target for an anticancer strategy. Furthermore, previous studies showed that molecules of the cisplatin family, used as chemotherapeutic agents, led to the destruction of the mitochondrial membrane potential and thus to cell death. Does the anticancer effect of these molecules result, at least partially, from this mitochondrial aggression ? If it is the case, the ANT2 isoform, mainly involved in the generation of this potential by its ATP4–/ADP3– exchange, could be considered as a more specific targeting by an RNA interference approach
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