72 research outputs found
Evidence that the V kappa III gene usage is nonstochastic in both adult and newborn peripheral B cells and that peripheral CD5+ adult B cells are oligoclonal.
No full textInternational audiencehere is evidence that in certain situations the expressed antibody repertoire is dominated by small subsets of V gene segments. They include fetal, CD5+, and autoantibody-forming B cells as well as low grade B cell malignancies. For instance, inside the V kappa III family of approximately 10 members, only 3 (humkv325, 328, and Vg) are used recurrently for autoantibody production. However, the significance of this recurrence is difficult to interpret without a clear vision of the actual repertoire in normal subjects. To address this, we have sequenced and compared two sets of rearranged V kappa III genes generated by cDNA PCR amplification from a normal newborn, a normal adult, and from CD5+ B cells of the same adult donor. The results show that: (a) only four V kappa III gene segments are used by neonatal and total adult B cells (humkv325, humkv328, Vg, and kv305), humkv325 being overexpressed in both repertoires; (b) there is no significant difference in terms of V kappa III gene usage between the adult and newborn repertoires; (c) regarding the junction regions, there is a favored use of the most 5' JK gene segments (Jk1-Jk2); approximately 20% of the newborn and adult junction sequences was characterized by one or two additional codons, most probably resulting from a nontemplate addition of nucleotides; (d) adult clones, in contrast to most newborn clones, show sequence divergences from prototype sequences with patterns which suggest antigen-driven diversity; (e) regarding the adult CD5+ B cell library, it is most probable that the 78 clones analyzed derived from no more than nine different VK-JK rearrangements. Humkv325 is used by at least six of them, and most of the expressed V genes were in exact or very near germline configuration. Collectively these results suggest that the expressed antibody V kappa III repertoire in the adult represents only a fraction of the potential genetic information and that it resembles the preimmune repertoire of the neonate. The data, which also suggest that the adult peripheral blood CD5+ B cell population may be dominated by a small number of B cell clones, are discussed with regards to the V kappa III usage in pathological situations
Rev Med Interne
No full textIntroduction La consommation de cocaïne est associée à de multiples complications dont certaines peuvent mimer des maladies systémiques, particulièrement des vascularites associées aux Anticorps Anti-Cytoplasme des Neutrophiles (ANCA). Nous rapportons un cas de Cocaine Induced Midline Destructive Lesions (CIMDL) ayant fait discuter un diagnostic différentiel de granulomatose avec polyangéite (GPA). Observation Un homme de 42 ans, cocaïnomane, était hospitalisé pour bilan de lésions destructrices centro-faciales. Il n'avait pas d'atteinte extra-ORL. Biologiquement, il existait des ANCA de fluorescence périnucléaire en immunofluorescence indirecte et de spécificité anti-Protéinase 3 (immunodosage par chimiluminescence). Cette présentation immunologique particulière associée à l'absence d'argument histologique pour une GPA faisait retenir un diagnostic de CIMDL. Le patient était perdu de vue. Conclusion Les CIMDL correspondent à des lésions destructrices centro-faciales induites par la consommation de cocaïne par voie nasale. Elles sont fréquemment associées à la présence d'ANCA. Une fluorescence périnucléaire avec une double spécificité anti-HNE et anti-PR3 est évocatrice du diagnostic.Introduction Cocaine use is associated with multiple complications, some of which can mimic systemic diseases, especially Antineutrophil Cytoplasmic Antibody (ANCA) associated vasculitis. We report a case of Cocaine Induced Midline Destructive Lesions (CIMDL) for which a diagnosis of granulomatosis with polyangiitis (GPA) was discussed. Case report A 42-year-old male, cocaine consumer, was admitted in our department for a centrofacial destructive process. He had no extra ear, nose and throat (ENT) involvement. ANCA were positive with a perinuclear fluorescence pattern and an anti-Proteinase 3 specificity. Regarding this unusual immunologic pattern and in the absence of histological argument for a GPA, a diagnosis of CIMDL was made. Conclusion CIMDL is a centrofacial destructive process due to intranasal cocaine use. It is frequently associated with the presence of p-ANCA with both anti-HNE and anti-PR3 specificity
FRI0346 Neutropenia in systemic lupus: prevalence, specific features and clinical consequences. results from the large upper rhine database lbbr
No full textDysfonctionnement des barorécepteurs carotidiens après endartériectomie bilatérale simulant un phéochromocytome
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Seasonal variability of bull and tiger shark presence on the west coast of Reunion Island, western Indian Ocean
No full textA fisheries-independent survey using longlines and drumlines, and an acoustic telemetry study, revealed that bull sharks Carcharhinus leucas and tiger sharks Galeocerdo cuvier occur throughout the year off the west coast of Reunion Island. The research, which commenced in 2011, was conducted in response to an increase in the rate of shark attacks. Juvenile, subadult and young adult tiger sharks of 111-390 cm total length (TL) were caught in all months except July; the sex ratio was 1:1 (M:F; n = 61). All bull sharks taken, except one (183 cm TL), were mature (205-329 cm TL), with a sex ratio of 0.7:1 (M:F; n = 54), and catches occurred throughout the year except in May and August, with the highest CPUE in September. Presence/absence of a total of 46 tagged tiger sharks and 36 tagged bull sharks was monitored by means of 42 acoustic receivers distributed along the west coast of the island. Tagged tiger sharks were detected in all months, with seasonal variation between sexes. Detections of males remained low throughout the year but with a peak in winter, whereas detections of females were low in May and August only and peaked in summer. Tagged bull sharks of both sexes were more abundant in winter than in summer, with females present year round. The reasons for the apparent differences in seasonality found in longline and drumline catches compared to acoustic monitoring are discussed
Découverte d’un myxome de l’oreillette gauche dans un contexte clinique évoquant une spondylarthropathie
No full textRhabdomyolyse causée par la simvastatine chez un transplanté cardiaque sous ciclosporine
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Les cellules des lymphomes MALT des glandes salivaires compliquant le syndrome de Sjögren expriment un facteur rhumatoïde
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