2,228 research outputs found
Development of in-cell NMR methodologies
No full textThe characterization of molecules within a biologically relevant environment distinguishes nuclear magnetic resonance (NMR) spectroscopy from other molecular-based biophysical techniques, such as X-ray crystallography and cryo-electron microscopy. Due to its exceptional stability and reduced ability to interact in a specific manner with other cellular components, the GB1 protein represents the quintessential probe to investigate the physiochemical effects imposed by the crowded environment on the structure and dynamics of proteins, without simultaneously compromising the ability to obtain in-cell NMR spectra due to binding events.
The general aim of this thesis was to investigate the possible interactions of the GB1 protein with the Escherichia coli lysate and intracellular milieu with the purpose of inferring the physiochemical effects imposed by these two crowded environments on the structure and dynamics of proteins. Thus, the experimental parameters critical for performing in-cell NMR experiments, including bacterial growth and protein overexpression within E. coli cells, were initially optimized. Subsequently, by monitoring proton and nitrogen chemical shifts of backbone amides and lysines side chains, as well as carbon and proton chemical shifts of side chains containing carbonyl groups, the preferential behaviour of GB1 was analysed in lysate and within cells, considering the pure protein in water as the reference state. Furthermore, interactions with the local environment were further examined by determining the overall translational motion of the protein through diffusion-ordered NMR spectroscopy (DOSY). The results obtained suggest that the intracellular environment is much more viscous than its artificially crowded counterpart and that GB1 exhibits a distinct behaviour in E. coli than in lysate. Specifically, residues at or near the more flexible and solvent-exposed loop regions of the protein display an increased preference for interaction with cellular components within cells compared to lysate. Finally, a comparison of diffusion coefficients obtained with DOSY and fluorescence correlation spectroscopy (FCS), the standard analytical technique for studying protein diffusion, was made
Sinergias entre autoavaliação e avaliação externa de escolas
No full textSeminários realizados na Universidade de Évora, de 26-27 de novembro de 2021, e organizados por: Isabel Fialho (Presidente); Ana Paula Correia; Helena Bruno; Hugo Rebelo; José Saragoça; Marcelo Coppi; Maria José Silvestre; Sílvia Nogueira; Sónia Gomes; Carlos Barreira; Filipa Seabra; José Augusto Pacheco.info:eu-repo/semantics/publishedVersio
Marcelo dascal and the literal meaning debates
No full textWhat role does literal meaning play in people’s understanding of indirect and figurative language? Scholars from many disciplines have debated this issue forseveral decades. This chapter describes these debates, especially focusing on the arguments between the author and Marcelo Dascal. I suggest that Dascal’s defense of “moderate literalism” may have some validity, contrary to some of my earlier arguments against this point of view. The chapter acknowledges the strong contribution that Marcelo Dascal has made to interdisciplinary discussions on language and thought
Marcelo Cohen’s Work : Between Fantastic Sociology and Fictional Geography
No full textCette thèse étudie les notions de géographie imaginaire et de sociologie fantastique dans l’œuvre de Marcelo Cohen (Buenos Aires, 1951). Le corpus de travail comprend l’ensemble de la production de fiction narrative de l’auteur (contes, nouvelles et romans en une vingtaine d’ouvrages) entre son premier recueil de récits, Lo que queda (1972), et son roman Balada (2011). La thèse est organisée en trois parties : la première (« Marcelo Cohen et son œuvre. Perspectives théoriques et méthodologiques ») propose une présentation de l’auteur et de son œuvre et un bilan théorique et pratique des notions de géographie imaginaire et de sociologie fantastique ; la deuxième (« Écrire l’espace ») analyse la construction de l’espace dans le corpus choisi dans une double démarche chronologique et figurative ; la troisième (« Littérature et société ») se penche sur les manifestations des dimensions idéologiques, sociales et politiques, et sur les relations entre l’individu et la société dans les ouvrages de Marcelo Cohen. Le travail s’achève par une réflexion globale sur la poétique et l’esthétique de l’auteur argentin.This thesis considers such concepts as fictional Geography and fantastic Sociology in Marcelo Cohen’s work (Buenos Aires, 1951).The body of this research includes all the narrative fiction by this author (some twenty tales, short stories and novels in total), between his first collection of stories, Lo que queda (1972), and his novel Balada (2011).Our thesis develops three domains : the first one (« Marcelo Cohen and his work, a theoretical and methodological outlook ») introduces the author and his work, along with a theoretical and practical evaluation of such concepts as fictional Geography and fantastic Sociology ; the second one (« Space in writing ») analyzes the make of space in the selected books, in a dual approach : both chronological and representational ; the last one (« Literature and society ») addresses the expressions of ideological, sociological and political dimensions, plus the relationship between a particular person and his/her society in Marcelo Cohen’s works. This research ends with a global consideration of poetry and estheticism in the work of our Argentinean author
Financiamento partidário: como se alteram as contas dos partidos políticos num contexto de recessão económica / Daniela Filipa Monteiro Vilela
No full textEm contextos de recessão económica, acentua-se a desconfiança dos cidadãos perante as diversas instituições da sociedade, e onde estão incluídos os Partidos Políticos. Em prol de um financiamento político transparente, a regulamentação e a fiscalização são cada vez mais imprescindíveis. Deste modo, a qualidade do financiamento partidário tem ganho cada vez mais importância nos sistemas políticos, e nas sociedades democráticas.
Esta dissertação, centra-se na temática do financiamento partidário, respetivos órgãos de fiscalização, prestação de contas e quadro regulamentar. Pretende-se efetuar uma comparação do financiamento partidário vigente, antes e depois da crise económica e com referencia às alterações na Lei. Neste sentido, serão analisados os rendimentos dos partidos políticos portugueses e orçamentos de campanha, de modo a verificar em que medida foram registadas alterações ao longo dos anos, tendo em conta diversos indicadores sociais potenciados pela crise económico-financeira.In an economic downturn context, the citizens distrust about the various institutions of society, where the political parties are included, get increased. In favour of a more transparent political financing regulation and supervision play a key part in the development of the system. Thus, the quality of party financing has gained increasing importance in political systems, and democratic societies.
This dissertation will also address the changes that occurred in the Portuguese legislation regarding the mater. It is intended to make a comparison of the current party financing before and after the economic crisis, with reference to changes that may have occurred in the law. This study will also analyse the financial incomes and campaign budgets of the Portuguese political parties. In order to verify to what extent were recorded changes over the years, taking into account various social indicators boosted by the economic and financial crisis
Financiamento partidário: como se alteram as contas dos partidos políticos num contexto de recessão económica / Daniela Filipa Monteiro Vilela
No full textEm contextos de recessão económica, acentua-se a desconfiança dos cidadãos perante as diversas instituições da sociedade, e onde estão incluídos os Partidos Políticos. Em prol de um financiamento político transparente, a regulamentação e a fiscalização são cada vez mais imprescindíveis. Deste modo, a qualidade do financiamento partidário tem ganho cada vez mais importância nos sistemas políticos, e nas sociedades democráticas.
Esta dissertação, centra-se na temática do financiamento partidário, respetivos órgãos de fiscalização, prestação de contas e quadro regulamentar. Pretende-se efetuar uma comparação do financiamento partidário vigente, antes e depois da crise económica e com referencia às alterações na Lei. Neste sentido, serão analisados os rendimentos dos partidos políticos portugueses e orçamentos de campanha, de modo a verificar em que medida foram registadas alterações ao longo dos anos, tendo em conta diversos indicadores sociais potenciados pela crise económico-financeira.In an economic downturn context, the citizens distrust about the various institutions of society, where the political parties are included, get increased. In favour of a more transparent political financing regulation and supervision play a key part in the development of the system. Thus, the quality of party financing has gained increasing importance in political systems, and democratic societies.
This dissertation will also address the changes that occurred in the Portuguese legislation regarding the mater. It is intended to make a comparison of the current party financing before and after the economic crisis, with reference to changes that may have occurred in the law. This study will also analyse the financial incomes and campaign budgets of the Portuguese political parties. In order to verify to what extent were recorded changes over the years, taking into account various social indicators boosted by the economic and financial crisis
Transcrição e fixação do texto por Isabel Almeida, Filipa Araújo, Manuel Ferro, Teresa Nascimento, Marcelo Vieira. Notas por Isabel Almeida, Filipa Araújo, Marcelo Vieira. Nota introdutória, índices e revisão por Isabel Almeida
No full textEsta edição pretende dar a conhecer os comentários feitos pelo crúzio D. Marcos de S. Lourenço (? – c. 1645) ao poema épico de Camões. Desses comentários (se de facto chegaram a ser concluídos) restará hoje apenas uma cópia manuscrita e imperfeita: o códice 46-VIII-40 da Biblioteca da Ajuda, onde não se encontram senão os escólios aos três primeiros cantos do poema épico, seguidos de mais algumas notas, decerto destinadas à prossecução do trabalho. Pelas datas registadas no manuscrito, sabemos que terá sido elaborado entre 1631 e 1633, inteira ou principalmente no convento de Torre de Paderne, perto de Melgaço. D. Marcos, que nasceu em Coimbra, em data incerta, viveu alguns anos em Lisboa (aí se encontraria quando, em 1619, Filipe III de Espanha veio a Portugal), antes de chegar ao Alto Minho. Terá também passado por Santa Cruz de Coimbra, mas desconhece-se, com rigor, o itinerário deste cónego de Santa Cruz, que leu Os Lusíadas com paixão.
Além da edição anotada d’Os Lusíadas Comentados por D. Marcos de S. Lourenço, o volume publicado pelo CIEC oferece uma bibliografia, acompanhada de um índice onomástico e toponímico, bem como (graças à generosa licença concedida pela Biblioteca da Ajuda) um CD-Rom com a reprodução do manuscrito
Urgent Writings. On science, education and justice. Reflections on the neoliberal advance
No full textBook review: "Escritos Urgentes. On science, education and justice. Reflexiones ante el avance neoliberal". Author: Grupo CyTA Year 2017. Editorial: Punto de Encuentro. Origin: Argentina By Marcelo Rui
V Congresso nacional de educação para a saúde – Livro de resumos
No full textLivro de resumos do V Congresso Nacional de Educação para a Saúd
Marcelo Cohen. Las fundaciones de la ciencia ficción
No full textThe present work analyzes the forms that Science fiction takes in Marcelo Cohen´s narrative. By outlining certain characteristics of the genre, it is considered the ways how these are reformulated according to the Romantic epistemological paradigm that the author picks up, blending it with concepts of quantum and chaos theories, to postulate a model of Realism. Within this context, the initial analysis of the author´s self-fashioning and essays is useful to think the proposal of a singular ontology in his fictions
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