1,720,968 research outputs found
Edukasi Dan Pemeriksaan Golongan Darah Sistem ABO Pada Siswa Sekolah Dasar
Full text linkGangguan pada darah dapat mempengaruhi proses metabolisme tubuh dan mengakibatkan terjadinya penyakit gagal ginjal serta kesalahan transfusi darah. Pemeriksaan golongan darah yang tidak tepat sebelum transfusi menyebabkan reaksi imunologis seperti anemia hemolitik, syok dan kematian. Pemeriksaan golongan darah dilakukan agar masyarakat mengetahui jenis golongan darah untuk antisipasi jika membutuhkan transfusi darah. Tujuan pengabdian kepada masyarakat ini untuk memberikan edukasi dan mengetahui jenis golongan darah siswa Sekolah Dasar melalui pemeriksaan golongan darah sistem ABO. Tahap persiapan dilakukan observasi dan sosialisasi. Tahap pelaksanaan terdiri dari edukasi, diskusi tanya jawab dan pemeriksaan golongan darah. serta evalusi pada tahap akhir. Edukasi sangat efektif memberikan pemahaman kepada siswa tentang pentingnya mengetahui golongan darah. Jumlah siswa golongan darah O sebanyak 67 orang (52%), golongan darah A sebanyak 21 orang (16%), golongan darah B sebanyak 34 orang (26%) dan golongan darah AB sebanyak 8 orang (6%)
Spatial analysis of toxoplasmosis through EcoHealth approaches using GRA-1 recombinant: case in Sleman, Yogyakarta
Full text linkToxoplasmosis is an obligate intracellular zoonotic parasite caused by Toxoplasma gondii that can infect all warm-blooded animals including humans. Prevalence of toxoplasmosis varies depending on climate, geography, and the presence of cats in an area. This study aimed to identify the prevalence and distribution of toxoplasmosis in Sleman, Yogyakarta through EcoHealth approaches. A total of 385 blood samples were collected from residents in the district of Sleman. Seven people from 55 villages were selected for blood sampling using a cluster method. The collected serums were tested by ELISA using recombinant Granule 1 protein (GRA-1) as coated antigen. Data on altitude and coordinates of sampling sites were collected using GPS. instruments, soil surface temperature in Sleman was obtained by satellite imagery, and cat population in residential areas was determined by questionnaire. The prevalence of toxoplasmosis in Sleman was 58%, of which distributed around rivers and in cattle pens. Based on altitude and temperature, toxoplasmosis cases were found the highest at 0-150 m (66.3%) and at temperatures of 26-30°C (66.4%). Areas with large numbers of cats had toxoplasmosis prevalence of 75.8% while areas with moderate and few cats were 56.5% and 49.0%, respectively. Thus, differences in the prevalence of toxoplasmosis at settlement were found based on altitude, soil surface temperature, and cat populations
PEMANFAATAN SAMPAH ORGANIK RUMAH TANGGA SEBAGAI PAKAN FERMENTASI HEWAN TERNAK
Full text linkABSTRAKSampah dan pengelolaannya menjadi masalah yang sangat penting, jika tidak dikelolah dengan baik dapat mengakibatkan terjadinya perubahan keseimbangan lingkungan. Sampah rumah tangga yang dihasilkan oleh masyarakat di desa Karang Bayan biasanya hanya dibakar, sehingga dapat terjadi pencemaran lingkungan seperti polusi udara dan lingkungan menjadi kotor serta dapat menimbulkan masalah kesehatan bagi masyarakat. Mata pencaharian sebagian besar penduduk Desa karang bayan adalah petani dan peternak. Pengabdian kepada masyarakat ini dilakukan untuk mengatasi permasalahan sampah rumah tangga di desa Karang Bayan menjadi bahan yang mempunyai nilai ekonomis yaitu pakan ternak. Pengolahan pakan ternak secara fermentasi berguna untuk meningkatkan produktivitas hewan ternak. Kegiatan pengabdian kepada masyarakat dilakukan dengan metode penyuluhan, demontrasi dan latihan. Hasil kegiatan di penyuluhan yang diberikan, masyarakat Desa Karang Bayan mengetahui dampak negatif sampah rumah tangga yang tidak dikelolah dengan baik terhadap kesehatan dan lingkungan, masyarakat mengetahui jenis sampah organik dan anorganik serta mampu melakukan pemisahan kedua jenis sampah rumah tangga tersebut dan masyarakat dapat mengolah sampah organik rumah tangga menjadi pakan ternak dengan metode fermentasi secara mandiri. Pakan fermentasi yang dibuat sudah dimanfaatkan oleh beberapa peternak di Desa Karang Bayan sebagai pakan terutama untuk ternak unggas. Kata kunci: sampah organic; masalah kesehatan; pakan fermentasi. ABSTRACTWaste and its management become a very crucial problem, if not managed properly it can lead to changes in the environmental balance. Household waste produced by the community in Karang Bayan village is usually only burned, so that environmental pollution can occur such as air pollution and the environment becomes dirty and can cause health problems for the community. The livelihoods of most residents of Karang Bayan Village are farmers and ranchers. This community service is carried out to overcome the problem of household waste in Karang Bayan village into material that has economic value, that is animal feed. Fermentation of animal feed processing is useful for increasing the productivity of livestock. Community service activities are carried out using counseling, demonstration and training methods. The results of the activities from the counseling given to the people of Karang Bayan Village to find out the negative impact of household waste that is not managed properly on health and the environment. The community knows the types of organic and inorganic waste and is able to separate the two types of household waste and the community can process household organic waste into animal feed with the fermentation method independently. Fermented feed has been used as animal feed in Karang Bayan Village, especially for poultry. Keywords: organic waste; helath problem; fermented feed
ACCURATE DETECTION OF VIRAL SEROTYPE DENGUE HEMORRHAGIC FEVER THROUGH AEDES SP MOSQUITOES USING REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION (RT-PCR)
Full text linkDengue Hemorrhagic Fever (DHF) caused Dengue virus which transmitted through the bite of the Aedes sp. Detection of dengue virus serotypes is essential for epidemiology as well as potential disease pathogens. RT-PCR method was more effective in mosquitoes, because the virus contained in the mosquito body is passed on to the next generation (trans ovarian). The purpose of this research is to know the serotype of DHF virus accurately through vector mosquito using RT-PCR method in Mataram City, so that vector control, early diagnosis and management of DHF disease could be done quickly and precisely. This research was a laboratory explorative research with cross sectional design that determines serotype of DHF virus through vector mosquito using RT-PCR method in Mataram City. The population of this study were Aedes aegypti female mosquitoes from eggs. Collected from houses located in Community Health Center Mataram city worked area with high dengue cases: Pagesangan, Karang Taliwang, Karang Pule, Tanjung Karang, Mataram, and Selaparang especially around the house’s sufferers. The results showed that only the mosquito group from the worked area of the Mataram Community Health Center showed the ribbon image with the base pair in accordance with serotype 1 (DENGUE 1), the other negative
Korelasi Kadar C-Reaktif Protein dengan Persentase Eosinofil pada Pasien Covid-19
Full text linkSevere Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) merupakan virus corona baru yang dikenal sebagai Covid-19. Pemeriksaan skrining seperti C-reaktif protein dapat dilakukan pada saat timbulnya gejala klinis. infeksi SARS-CoV-2 dapat menyebabkan peradangan yang mengakibatkan meningkatnya kadar C-reaktif protein. Peradangan dapat diketahui dengan pemeriksaan hitung persentase eosinofil dimana sel ini berperan dalam sistem kekebalan tubuh terhadap infeksi virus. Penelitian ini bertujuan mengetahui adanya korelasi antara kadar C-reaktif protein dengan persentase eosinofil pada pasien Covid-19. Penelitian ini merupakan penelitian survey analitik menggunakan rancangan survey cross sectional dengan pendekatan observasi. Teknik pengambilan sampel adalah accidental sampling. Sebanyak 24 sampel dari penderita Covid-19 dilakukan pemeriksaan eosinofil dan kadar C-reaktif protein. Uji statistik hasil pemeriksaan dilakukan analisis bivariate dengan rumus korelasi pearson. Hasil penelitian didapatkan rerata eosinofil yaitu 2,5% dan rerata kadar C-reaktif protein 13,56 mg/L. Hasil uji statistik didapatkan nilai p= 0,037<0,05 dan nilai r= -0,428 sehingga menunjukkan nilai yang signifikan dan korelasi antara persentase eosinofil dengan kadar C-reaktif protein. Terdapat korelasi antara penurunan persentase eosinofil dengan peningkatan kadar C-reaktif protein pada pasien Covid-19
IMPLEMENTASI COMMUNITY-BASED PARSIPATORY DAN PEMERIKSAAN ANTI-TB UNTUK DETEKSI DINI TUBERKULOSIS
Full text linkAbstrak: Kasus tuberkulosis masih banyak ditemukan tetapi pemahaman masyarakat tentang tuberkulosis masih rendah, sehingga diperlukan upaya preventif untuk mencegah peningkatan kasus tuberkulosis. Pengabdian kepada masyarakat ini bertujuan untuk meningkatkan pemahaman masyarakat tentang tuberkulosis dan deteksi dini tuberkulosis melalui pendekatan Community-Based Parsipatory dengan melibatkan kader dan peran aktif masyarakat. Metode yang digunakan terdiri dari sosialisasi, penyuluhan, edukasi, diskusi dan tanya jawab, pembentukan kelompok (Community-Based Parsipatory) peduli tuberkulosis dan pelatihan, deteksi dini anti-TB serta monitoring evaluasi. Sasaran pengabdian kepada masyarakat sebanyak 50 orang terdiri dari 25 orang warga masyarakat dan 25 orang kader kesehatan desa, sedangkan sebagai mitra ialah petugas laboratorium Puskesmas dan staf desa. Hasil evaluasi menunjukkan bahwa penyuluhan efektif dapat meningkatkan pemahaman sasaran tentang tuberkulosis, sebelum penyuluhan pemahaman sasaran sebesar 20% dan sesudah penyuluhan meningkat menjadi 95%. Hasil monitoring yang dilakukan setiap bulan, ditemukannya satu orang anggota penderita tuberkulosis positif antibodi IgG yang menunjukkan bahwa anggota kelompok masyarakat (Comunity-Based Parsipatory) peduli tuberkulosis berperan aktif melakukan edukasi untuk mencegah penularan tuberkulosis.Abstract: Tuberculosis cases are still widely found but public understanding of tuberculosis is still low, therefore preventive efforts are needed to prevent an increase of tuberculosis cases. This community service aims to improve public understanding about tuberculosis and early detection of tuberculosis through a Community-Based Participatory approach involving village health preceptor and active community participation. The methods used consist of socialization, counseling, education, discussion, interview, formation group of Community-based participatory concerned with tuberculosis, training, early detection of anti-TB, monitoring and evaluation. The target of community service is 50 people consisting of 25 community members and 25 village health Preceptor, while as a Partners of this pragram are laboratory officers of community health centers and village staff. The evaluation results show that counseling can improve understanding of participant about tuberculosis effectively, the understanding of participant before counseling was 20% and increased to 95% after being given counseling. The results of monitoring carried out every month, found one member of the tuberculosis patient who tested positive for IgG antibodies, which shows that members of the community-based participation group who care about tuberculosis play an active role in providing education to prevent the transmission of tuberculosis.
Variasi Temperatur Boiling pada Amplifikasi Gen inhA M.tuberculosis Metode PCR
Full text linkPolymerase Chain Reaction (PCR) adalah metode yang direkomendasikan oleh world health organization (WHO) untuk pemeriksaan infeksi tuberkulosis dengan sensitivitas dan spesifisitas yang tinggi. Biaya yang murah, proses yang mudah, dan tehnik ekstraksi DNA yang cepat sangat diperlukan untuk mendapatkan DNA template. Salah satu cara untuk mendapatkan DNA template adalah metode boiling, dimana metode ini dapat meningkatkan permeabilitas dan merusak dinding sel hanya dengan pemanasan dalam waktu relatif singkat. Penelitian ini bertujuan untuk mengetahui pengaruh variasi suhu boiling 85°C, 90°C, dan 95°C masing-masing selama 30 menit, 10 menit dan 5 menit terhadap hasil amplifikasi gen inhA M. tuberculosis menggunakan metode PCR. Penelitian ini merupakan penelitian True Experiment dengan rancangan posttest only control design. Sampel yang digunakan dalam penelitian ini adalah dahak penderita TB yang di ekstraksi dengan metode boiling dan dibandingkan dengan metode ekstraksi kit. Hasil amplifikasi di elektroforesis pada gel agarosa 1% yang diamati dengan alat UV transiluminator. Hasil penelitian menunjukkan bahwa ekstraksi sampel sputum dengan boiling pada suhu 850C selama 30 menit, 900C selama 10 menit dan 950C selama 5 menit masing-masing ditemukan pita DNA gen inhA M.tuberculosis dengan panjang 465 bp. Ekstraksi sampel sputum dengan metode boiling dapat digunakan untuk pemeriksaan gen inhA M.tuberculosis metode PCR
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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