1,582,888 research outputs found
Animal models of skin cancer: the example of a chemically- induced rat model
No full textFaustino-Rocha AI. 2023. Animal models of skin cancer: the example of a chemically- induced rat model. 3rd Global Virtual Congress on Cancer research & Drug Development, 15 de maio
Chemical carcinogens: an overview.
No full textFaustino-Rocha AI. 2023. Chemical carcinogens: an overview. 8th Edition of Global Conference on Pharmaceutics and Novel Drug Delivery Systems, 13 a 14 de mai
Are animal models important? An overview of animal models of cancer
No full textFaustino-Rocha AI. 2023. Are animal models important? An overview of animal models of cancer. International Conference on Veterinary Science, 28 a 29 de agosto
Addressing new therapeutic strategies using models
No full textA disease model displays pathological processes observed in human or animal diseases. The models are fundamental tools in biomedical research, and they are invaluable for providing new insights into mechanisms underlying organ function, to establish the pathophysiology of a disease, and to test potential therapeutic approaches.
Models have been long used to study several diseases, including cardiovascular, oncologic, metabolic, infectious, and neurological diseases, among others. Animals or cells displaying pathological processes observed in human or animal diseases have been used as models. Moreover, mathematical models are also of great importance to disease modeling. Animal models offer the unique opportunity to investigate the function of genes and pathways and the in vivo effects of drugs, bridging the gap between basic science and the treatment of diseases. The selection of a suitable model system is a crucial step in research design. For disease modeling to be meaningful, a relevant cellular or genetic phenotype must be observed. Without the use of models, both research and clinical practice worldwide would be vastly different today.
This Special Issue aims to publish original research works or reviews with models of disease, highlighting their importance to search for new therapeutic strategies
Prostate cancer
No full textThe prostate is the largest accessory gland of the male reproductive tract. Together with seminal vesicles and bulbourethral glands, the prostate is responsible for the production of an alkaline fluid that forms part of the seminal fluid. The prostate of men over 40 years of age is commonly affected by several pathologies, such as benign prostate hyperplasia and cancer.
Prostate cancer is one of the most frequent cancers among the male population worldwide. According to the World Health Organization (WHO), in the year 2020, prostate cancer affected approximately 1.41 million men and was responsible for the death of 375,304 of them. Prostate cancer development is associated with several risk factors including older age, black ethnicity, a family history of the disease, an increased body mass index, and obesity. The risk of prostate cancer development may be reduced through the consumption of a healthy diet full of fruits and vegetables, practice of physical exercise, and maintenance of a healthy weight.
Despite several approaches being available for prostate cancer treatment, the number of prostate cancer deaths is continuously increasing, which emphasizes the need to search for new methods for precocious diagnosis and more effective treatment. Animal models including rodents have greatly contributed to the study of biopathology and the prevention and treatment of prostate cancer.
This Special Issue entitled “Prostate Cancer II” aims to publish original research works and reviews concerning the diagnosis, treatment, and prognosis of prostate cancer, highlighting new advances in this field
José Faustino Sánchez Carrión y la identidad faustiniana
No full textObjetivos: Explicar cómo la valoración de José Faustino Sánchez Carrión contribuye a afirmar la identidad institucional entre los docentes, estudiantes y trabajadores de la Universidad Nacional José Faustino Sánchez Carrión. Materiales y Métodos: Enfoque cualitativo, de tipo no experimental y de nivel exploratorio, descriptivo y explicativo; considerando el nivel de conocimiento y valoración de José Faustino Sánchez Carrión y su contribución a la identidad institucional. Resultados: Un significativo número de docentes, trabajadores administrativos y estudiantes de la Universidad conocen y valoran insuficientemente a José Faustino Sánchez Carrión y como parte y elemento que contribuye a afirmar y consolidar la identidad institucional de la Universidad que lleva su nombre. El Conclusiones: significativo desconocimiento y valoración de José FaustinoSánchez Carrión por parte de los docentes, trabajadores administrativos y estudiantes de la Universidad y por lo mismo como elemento que contribuye a afirmar la identidad institucional amerita la adopción de medidas adecuadas y oportunas para revertir esta situación.Palabras clave: José Faustino Sánchez Carrión, universidad, identidad instituciona
ESTRATÉGIAS PARA MAXIMIZAÇÃO OU PRESERVAÇÃO DO POTENCIAL REPRODUTIVO DE FÊMEAS: DAS CÉLULAS-TRONCO GERMINATIVAS AOS FOLÍCULOS PRÉ-ANTRAIS
No full textRESUMOEstratégias para a maximização do potencial reprodutivo de fêmeas, bem como para a preservação de seus gametas são pontos chaves para as técnicas de Reprodução Assistida. Dessa forma, os objetivos deste trabalho foram caracterizar células-tronco germinativas femininas (OSC, oogonial stem cell) isoladas de ovários de camundongas senis (Fase 1), investigar a expressão e o efeito da adição do fator de crescimento queratinócito (KGF) em ovários caprinos (Fase 2), avaliar o efeito da interação entre o KGF e o kit ligant (KL) na foliculogênese inicial de cabras (Fase 3) e verificar os efeitos da vitrificação de tecido ovariano caprino sobre a integridade do DNA de oócitos inclusos em folículos pré-antrais (Fase 4). Na Fase 1, o número de OSC viáveis em ovários de camundongas com 3 e 20 meses foi similar. Após cultivo, oócitos foram gerados espontaneamente após a expansão das OSC de camundongas senis. Na Fase 2, após 7 dias de cultivo in vitro, a adição de KGF (1, 10, 50, 100 ng/mL) reduziu a porcentagem de folículos normais comparados com o controle não-cultivado (P<0,05), no entanto, essa percentagem não deferiu do controle cultivado (P>0,05). Após 1 ou 7 dias de cultivo, a porcentagem de folículos em crescimento aumentou em todos os tratamentos quando comparada ao controle não-cultivado (P<0,05). A ultraestrutura folicular foi preservada após 7 dias de cultivo em 1 ng/mL de KGF e sua expressão foi observada em todas as categorias foliculares, bem como em células da teca e do estroma. Ao avaliar a interação entre KGF e KL (Fase 3), foi observado que o cultivo reduziu a porcentagem de folículos morfologicamente normais e viáveis comparados com o controle não-cultivado (P<0,05). Em adição, uma maior porcentagem de folículos em crescimento e de folículos com maior diâmetro foi observada quando o KL foi adicionado sozinho ao meio de cultivo comparado com o controle cultivado (P<0,05). Ao verificar os efeitos da vitrificação de tecido ovariano sobre a integridade do DNA oocitário (Fase 4), uma menor porcentagem de folículos morfologicamente normais foi observada no tratamento vitrificação seguida de cultivo (P<0,05). A presença de dano na dupla fita de DNA, indicada pela γ-H2AX, foi observada em poucos oócitos e algumas células do estroma do ovário. Folículos com fragmentação do DNA foram encontrados em todos os grupos, porém sem diferenças significativas entre os tratamentos (P>0,05). Em conclusão, ovários de camundongas senis mantêm OSC que podem formar oócitos após cultivo in vitro, podendo ser uma grande perspectiva para animais de produção de grande valor genético, como fêmeas caprinas. A adição de baixa concentração de KGF, amplamente distribuída nos diferentes compartimentos dos ovários, ao cultivo in vitro de tecido ovariano caprino mantém a ultraestrutura folicularapós 7 dias de cultivo. Em nossas condições de cultivo, não foi possível observar a interação do sistema KGF-1/KL na foliculogênese inicial de cabras. O protocolo de vitrificação do tecido ovariano utilizado neste estudo não aumentou o dano no DNA de oócitos inclusos em folículos pré-antrais.Palavras-chave: Células-tronco germinativas. Folículos pré-antrais. Cultivo in vitro Criopreservação.ABSTRACTStrategies to improve female reproductive potential as well as to preserve their gametes are key points to the Assisted Reproduction technology. Thereby, the aims of this work were to characterize female germinative stem cells (OSCs, oogonial stem cell) isolated from aged mice ovaries (Phase 1), to investigate the expression and the effect of the addition of keratinocyte growth factor (KGF) in goat ovaries (Phase 2), to evaluate the effect of the interaction between KGF and kit ligant (KL) on initial folliculogenesis in goats (Phase 3) and to verify the effects of goat ovarian tissue vitrification on DNA integrity of oocytes enclosed in preantral follicles (Phase 4). At Phase 1 the number of viable OSCs was similar between ovaries of 3- and 20-month-old mice. After culture, oocytes were spontaneously generated after the expansion of OSCs from aged mice. At Phase 2, after 7 days of in vitro culture, the addition of KGF (1, 10, 50, 100 ng/mL) reduced the percentage of normal follicles compared to the non-cultured control (P<0.05), however, this percentage did not differ from the cultured control (P>0.05). After 1 or 7 days of culture, the percentage of growing follicles increased in all treatments compared to the non-cultured control (P<0.05). The follicular ultrastructure was preserved after 7 days of culture in 1 ng/mL KGF and its expression was observed in all follicular categories as well as in theca and stroma cells. Evaluating the interaction between KGF and KL (Phase 3), it was observed that culture reduced the percentage of morphologically normal and viable follicles compared to the non-cultued control (P<0.05). In addition, a higher percentage of growing follicles and follicles with larger diameter was observed when KL was added to the culture media alone, compared to cultured control (P<0.05). Verifying the effects of the ovarian tissue vitrification on DNA integrity of oocytes (Phase 4), a lower percentage of morphologicaly normal follicles was observed on the treatment vitrification followed by culture (P<0.05). The presence of damage on DNA double-strand, indicated by γ-H2AX, was observed in just a few oocytes and in some ovarian stromal cells. Follicles with DNA fragmentation were found in all groups, without significative differences between the treatments (P>0.05). In conclusion, old mice ovaries maitain OSCs that can form oocytes after in vitro culture, being considered a great prospect for animals livestock of great genetic value, such as female caprine. The addition of low concentrations of KGF, which was widely distributed in different compartments of ovaries, to in vitro culture of goat ovarian tissue, keep the follicular ultrastructure after 7 days of culture. In our culture conditions, it was not possible to observe the interaction of the KGF-1/KLsystem in on initial folliculogenesis of goats. The protocol for ovarian tissue vitrification used in this study did not increase oocytes DNA damage enclosed in preantral follicles.Keywords: Oogonial stem cells. Preantral follicles. In vitro culture. Cryopreservation</div
Il culto dei santi Faustino e Giovita nell’Italia settentrionale
No full textLo studio prende in esame la diffusione del culto bresciano dei santi Faustino e Giovita che, in Italia settentrionale, si è espanso precocemente con testimonianze che partono dall’epoca carolingia attraverso le parrocchie legate a Brescia ma soprattutto attraverso le vaste proprietà, in primis del monastero di San Faustino ad esempio nella strategica Val Trompia, quindi di quelle collegate, come gli appannaggi territoriali di San Pietro in Monte di Civate verso il lago di Como, oppure quelli di San Benedetto di Leno, arrivate presumibilmente attraverso le dipendenze fino in Liguri
Tarjeta Postal de Faustino Ballvé a Pedro Dorado Montero
No full textTarjeta Postal de D. Faustino Ballvé, a D. Pedro Dorado Montero, sobre unos trabajos de aquel
Carta de Faustino Ballvé a Pedro Dorado Montero
No full textCarta de D. Faustino Ballvé, a D. Pedro Dorado Montero, solicitando nombres de criminalistas españoles para una revista alemana
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