110,684 research outputs found
Endo-permutation modules as sources of simple modules.
No full textThe source of a simple -module, for a finite -solvable group and an algebraically closed field of prime characteristic , is an endo-permutation module (see~\cite{Pu1} or~\cite{Th}). L. Puig has proved, more precisely, that this source must be isomorphic to the cap of an endo-permutation module of the form \bigotimes_{Q/R\in\cal S}\Ten^P_Q\Inf^Q_{Q/R}(M_{Q/R}), where is an indecomposable torsion endo-trivial module with vertex , and is a set of cyclic, quaternion and semi-dihedral sections of the vertex of the simple -module. At present, it is conjectured that, if the source of a simple module is an endo-permutation module, then it should have this shape. In this paper, we are going to give a method that allow us to realize explicitly the cap of any such indecomposable module as the source of a simple module for a finite -nilpotent group
The Dade group of a metacyclic -group.
No full textThe Dade group of a finite -group , formed by equivalence classes of endo-permutation modules, is a finitely generated abelian group. Its torsion-free rank equals the number of conjugacy classes of non-cyclic subgroups of and it is conjectured that every non-trivial element of its torsion subgroup has order , (or also , in case ). The group is closely related to the injectivity of the restriction map \Res:T(P)\rightarrow\prod_E T(E) where runs over elementary abelian subgroups of and denotes the group of equivalence classes of endo-trivial modules, which is still unknown for (almost) extra-special groups ( odd). As metacyclic -groups have no (almost) extra-special section, we can verify the above conjecture in this case. Finally, we compute the whole Dade group of a metacyclic -group
Torsion endo-trivial modules
No full textWe prove that the group T(G) of endo-trivial modules for a non-cyclic finite p-group G is detected on restriction to the family of subgroups which are either elementary abelian of rank 2 or (almost) extraspecial. This result is closely related to the problem of finding the torsion subgroup of T(G). We give the complete structure of T(G) when G is dihedral, semi-dihedral, or quaternion.CT
Effect of Glucose on Endo-xylanase and β-xylosidase Production by Fungi Isolated in Indonesia
No full textXylanases are widely produced by fungi, and the production of polysaccharide-degrading enzymes, in general, are usually subjected to carbon catabolite repression. In this work, the ability of several Indonesian indigenous fungi to produce endo-xylanase and β-xylosidase and their responses to glucose as a repressor were determined. Ten fungi were grown in a liquid medium supplemented with glucose as the repressor (0, 1%, 3%, and 5%), and the endo-xylanase and β-xylosidase productions were assayed. Aspergillus aculeatus FIG1 and A. oryzae KKB4 produced 3.85 and 0.70 U/mL of endoxylanase, respectively, compared with other strains (0.22 U/mL or less). Trichoderma asperellum PK1J2, T. virens MLT 2J2, A. aculeatus FIG1, T. asperellum MLT5J1, A. oryzae KKB4, and T. asperellum MLT3J2 produced 0.021-0.065 U/mL of β-xylosidase, whereas the other strains produced 0.013 U/mL or less of β-xylosidase. Adding 1% glucose to the growth medium can partially repress endo-xylanase production in A. aculeatus FIG1, T. asperellum PK1J2, and T. virens MLT4J1 and completely repress other strains. By adding 1% glucose, strains FIG1, PK1J2, and MLT4J1 suffered almost complete repression of β-xylosidase production, although such strains exhibited partial repression of endo-xylanase production. β-Xylosidase produced by the other strains showed complete repression by adding 1% glucose, except for A. aculeatus FIG1, A. tamarii FNCC 6151, and T. asperellum MLT1J1, which showed partial repression. Therefore, adding 3% glucose to the growth medium can result in complete repression of endo-xylanase and β-xylosidase productions in all strains examined. © The Author(s) 2022
Endo-Orco:GFP enrichment in the ciliary OS in kinesin-2 RNAi and mutant backgrounds.
No full text(A) Endo-Orco:GFP localisation inside the ab1-type s. basiconica from Control (Endo-Orco:GFP; +), and homozygous Klp64Dkj353 mutant (Endo-Orco:GFP; Klp64Dkj353) backgrounds at 6 days AE (a). Histograms depict total fluorescence intensity (mean ± S.D.) of Endo-Orco:GFP in Control, and Klp64Dkj353 mutant backgrounds at 6 days AE (b). The pairwise significance of difference was estimated using the two-tailed Student’s T-test, and p-values (*p s. basiconica. Jupiter:GFP localisation in the ab1-type s.basiconica from control (orcobGal4/+; Jupiter:GFP/UAS-Dicer), Klp64D RNAi (orcoGal4/UAS-Klp64D RNAi; Jupiter:GFP/UAS-Dicer) and Klp68D RNAi (orcoGal4/UAS-Klp68D RNAi; Jupiter:GFP/UAS-Dicer) background at 6 hours AE (a). Histograms depict total Jupiter:GFP fluorescence intensity (mean ± S.D.) in Klp64D and Klp68D RNAi backgrounds at 6 hours AE (b). The pairwise significance of difference was estimated using the one-way ANOVA test, p-values (*p (TIF)</p
Nobuzo Endo: interviews on February 7, March 20, 29, 30, and April 3, 1984
No full textTranscript (typescript, 141 pages) of a series of interview with Nebuzo Endo, a Japanese-American living in Utah in 1984. Mr. Endo (b. 1911) recalls being sent to Japan as a child for his education, Japanese culture, surviving and earthquake, and returning to live with his parents in Oakland, California during the Depression. He and his wife talk about their courtship and discuss Japanese marriage customs. Other topics covered include Judo, moving to Utah, farming, the Buddhist Church, and being Japanese during World War II
Overlay of the superimposed active site residues of; (a) <i>H. jecorina</i> Endo T, and <i>S. plicatus</i> Endo H (PDB ID 1EDT); (b) <i>H. jecorina</i> Endo T and <i>E. meningoseptica</i> Endo F1 (PDB ID 2EBN), (c) <i>H. jecorina</i> Endo T, and <i>E. meningoseptica</i> Endo F3 (PDB ID 1EOM), and (d) <i>H. jecorina</i> Endo T, and <i>B. thetaiotaomicron</i> Endo BT (PDB ID 3POH).
No full text<p>The active site residues of Endo T are depicted in orange and those of Endo F1, Endo H, and Endo F3 in red. Figure prepared with the program PyMol <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040854#pone.0040854-Brnger1" target="_blank">[52]</a>.</p
Structure-function study on the activation mechanism of Endo T : Crystallization and biochemical characterization of the intact Endo T from Trichoderma reesei (Hypocrea jecorina)
No full textEndo T is a deglycosylating enzyme secreted by the filamentous fungus Trichoderma reesei (Hypocrea jecorina). The active Endo T protein is both deglycosylated and C-terminal processed. Since the intact protein was never observed in the T. reesei culture medium, a construct of the intact Endo T was expressed in P. pastoris. Here, the glycosylated, intact and inactive protein was found in the medium but this form is slowly converted to the deglycosylated and proteolytic cleaved form. The structure of the active Endo T was already solved in previous work. We tried to solve the structure of the intact inactive Endo T to compare it with the active structure. Several structures of Endo T were solved, but none of them were structures of the intact Endo T. Different pH and T parameters were screened to find a condition where the C-terminal peptide was stable and the enzyme kept intact. A protease inhibitor cocktail was also added to limit the proteolytic process. We were able to control the proteolysis with the help of the protease inhibitor cocktail at pH 5 and higher, but we were not able to limit the proteolysis at pH 3, nor in any of the crystallization samples
Estudo do meio e das condições de cultivo para produção de endo-inulinase microbiana /
No full textDissertação (Mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico.O presente trabalho teve como principais objetivos investigar o desempenho de microrganismos potencialmente produtores de endo-inulinase e estudar as condições de produção desta enzima. Dezesseis linhagens fúngicas e três linhagens bacterianas foram avaliadas quanto a produção de endo-inulinase. Os resultados indicaram o microrganismo isolado Paenibacillus sp. CDB 003 como sendo o melhor produtor de endo-inulinase. A influência das concentrações de inulina, de extrato de levedura, da concentração de sulfato de amônio, de ácido succínico, do íon fosfato , assim como da freqüência de agitação, do suprimento de oxigênio (KLa), da temperatura de cultivo e do pH do meio sobre a produção de endo-inulinase foi avaliada. Sendo a inulina comercial um substrato de alto custo para a produção industrial de endo-inulinase, o comportamento da cepa Paenibacillus sp. CDB 003 foi investigado em meio contendo extrato de chicória como fonte natural de inulina. Os resultados encontrados foram similares àqueles obtidos com a inulina comercial. Algumas características da enzima, tais como, temperatura ótima de incubação, estabilidade com o tempo e com a temperatura, valores de Km e Vmax e a correlação entre o tempo de hidrólise da inulina e a formação de açúcares redutores também foram avaliados
Mutational engineering, growth selection, expression, purification and crystallization of ligand bound endo-beta-N-acetylglucosaminidase T (Endo-T) from Hypocrea jecorina
No full textRecently, one of the first fungal-expressed, deglycosylating, Endo-beta-N-acetylglucosaminidases was found in the extracellular medium of soft-rot ascomycete Hypocrea jecorina (a.k.a. Trichoderma reesei) belonging to glycoside hydrolase family 18 (GH18). It was named Endo-T and has been shown to possess similar substrate specificities as Endo-H from Streptomyces plicatus, a deglycosylating enzyme, frequently used in the field of glycoproteomics. In this study an oligomannosidic N-glycan was introduced in the active site of crystallized Endo-T under acidic pH conditions below pH 3. The ligand-containing crystal diffracted on a synchrotron x-ray source to a resolution of 1.65Å. The resulting Endo-T structure was found to contain a bound ligand consisting of a hydrolyzed Manα1-6(Manα1-3)Manα1-6Manβ1-4GlcNAc N-glycan, lacking the aspargine linked N-acetylglucosamine residue of N-glycans. Furthermore, electron density was missing for several of the distal glycone mannose residues. The anomeric carbon of the distal N-acetylglucosamine residue was found to be positioned 6.88Å from the proposed catalytic amino acid residue, Glu131, indicating a descending motion during hydrolysis. An unidentified electron density was found after the last structure refinement, apparently shaped as a furanose ring, next to the N-glycan ligand in the active site around unit 8 of the (β/α)8-barrel. Proximal substrate positioning and the loop-structure of Endo-T suggests aglycone docking centered over (β/α)8-barrel unit 5
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