447 research outputs found
Why feminist stories matter: Katy Deepwell interviews Clare Hemmings
No full textClare Hemmings is Professor of Feminist Theory and Director of the Gender Institute at the London School of Economics. She is the author of Why Stories Matter: The Political Grammar of Feminist Theory (Duke University Press, 2011). For this volume, Katy Deepwell interviewed her about her views on feminist historiography and feminist theory, which Hemmings has defined in terms of three dominant narratives about the direction of feminism’s past, present and future
From flesh to fiction : the visible and the invisible in the work of Maurice Merleau-Ponty, Eudora Welty and Elizabeth Bowen.
No full textPhDOur ways of thinking modernism and its legacy are imprinted with the pattern of an
opposition, a struggle between two sets of extremes: objective and subjective; form
and feeling; mechanistic and organic; mind and body; knowing and being; self and
world; aesthetic and historical. The three writers whose work I explore in this thesis
challenge prevailing notions of this oppositional discourse. Entering the scene of
modernism late in its history, Elizabeth Bowen, Eudora Welty and Maurice Merleau-
Ponty develop a new kind of vision that makes us rethink the relationships between
perceiver and perceived, between mind, body and world. All three writers undertake a
fundamental reorganisation of the relationships between internal consciousness and
external things through the narration of a perception that is outside the limits of
discrete sensations or causal relationships. Physical things are neither pure objecthood
nor merely external triggers for the ramblings of a solipsistic consciousness, rather
they infringe on a consciousness whose own edges are indistinct. This writing
establishes an interdependent and interlocutory relationship between subject and
world, which become not opposite ends of a perceptual scale, but aspects of a common
flesh. The intimate connection to the world is both comforting and threatening, both
reinforcing subjectivity and de-centring it.
The re-ordering of the connections between self and world leads to a reassessment of
collective identity and historical agency, as well as impacting upon approaches to
modes of representation. In trying to express the pre-linguistic experience of embodied
consciousness, this writing looks to models of mute expression found in visual images.
Exploring how the invisible aspects of experience emerge within the visible realm, the
writing takes on an often hallucinatory or uncanny character. Charting the passage
from being to doing, from perception to creation, from the style of the flesh to the style
of fiction, Merleau-Ponty, Welty and Bowen dissolve received boundaries and
distinctions at every level
The Problem That Isn’t Ours
No full text“The Problem That Isn’t Ours” is a 2015 Newcomb College Institute Senior Symposium lecture delivered and written by author Katy Smith.
Why feminist stories matter: Katy Deepwell interviews Clare Hemmings
No full textClare Hemmings is Professor of Feminist Theory and Director of the Gender Institute at the London School of Economics. She is the author of Why Stories Matter: The Political Grammar of Feminist Theory (Duke University Press, 2011). For this volume, Katy Deepwell interviewed her about her views on feminist historiography and feminist theory, which Hemmings has defined in terms of three dominant narratives about the direction of feminism’s past, present and future
The Opinion – Volume 24, No. 4, May 1982
No full textSelected Table of Contents Clinics: Hands on legal training / Katy Perry Mitchell trial team wins second in nation Crime rise prompts increased security / Jeanne Anderson Librarian resigns amidst problems / Jeanne Anderson Decision clarifies \u27aiding, abetting\u27 law / David Warg
Editorial Board
Chuck Friedman; George McCormick; Jeanne Anderson; Katy Perry; Deborah Ellis; Eileen Casalhttps://open.mitchellhamline.edu/the-opinion/1081/thumbnail.jp
The Opinion – Volume 24, No. 1, October 1981
No full textSelected Table of Contents Child Care: A Celebration / Katy Perry Three absences will be grounds for suspension / Chuck Friedman Flexibility goal as day program expands / Chuck Friedman Women faculty gain ground at Mitchell / Jeanne Anderson Changes spur interest in student loans / Michele DiEuliis
Editorial Board
Chuck Friedman; George McCormick; Jeanne Anderson; Katy Perry; Deborah Ellis; Eileen Casalhttps://open.mitchellhamline.edu/the-opinion/1077/thumbnail.jp
CHARACTERIZATION OF THE MECHANISM OF 6-PHOSPHOGLUCONATE DEHYDROGENASE FROM TRYPANOSOMA BRUCEI AND ITS INTERACTION WITH INHIBITORS BY ISOTHERMAL TITRATION CALORIMETRY
No full text6-Phosphogluconate dehydrogenase (6PGDH) converts 6PG to ribulose 5-phosphate and
concomitantly provides NADPH, inside the pentose phosphate pathway. Its presence has been
shown essential for growth of bloodstream form Trypanosoma brucei, a parasite responsible for
African trypanosomiasis and it may be considered a validated drug target in this protozoan. Drugs
are the principal means of intervention but new drugs are urgently needed for human African
trypanosomiasis. Strong inhibitors have been found against 6PGDH, which show some selectivity
versus the parasite enzyme compared the mammalian one. T. brucei 6PGDH shows only a 33%
amino acid identity with the mammalian 6PGDH even if their structures have a similar overall fold
and many residues nearest neighbours to the substrate are conserved. Regarding 6PGDH
mechanism two residues, one acting as an acid and the other as a base, are postulated to assist all
the three catalytic steps. These residues (E192 and K185 in the T. brucei) have been identified on
the basis of crystallographic evidence and site-directed mutagenesis.
Much remains to understand yet, for instance on which way at the end of the reaction the
protonation state of the two catalytic groups changes into the opposite to that at the beginning of the
reaction and on the homotropic cooperativity of the enzyme (homodimeric) and the induced enzyme
changes at the substrate binding. To elucidate this and the inhibition mechanism by some lead
compounds to develop as potential drugs, we have exploited isothermal titration calorimetry
(ITC).
ITC measurements were performed in a VP-ITC microcalorimeter and the data were fitted by
nonlinear least-squares fitting using OriginTM software. The number of H+ exchanged has been
measured after binding studies in different buffers. Binding was studied sometimes by fluorometry.
Recombinant T. brucei 6PGDH has been purified from an overexpressing E. coli strain. Kinetics of
the enzymes was studied spectrophotometrically, also in function of pH. Preparation of site-directed
mutants of the enzyme has been a complementary technique to obtain information on the enzyme.
cysteine reactivity has been assayed with the Ellmann reactant DTNB.
Binding of the substrate and its analogues is entropy driven, while binding of coenzymes is
enthalpy driven. Oxidized coenzyme and its analogue display a half-site reactivity in the ternary
complex with the substrate or the inhibitors while reduced coenzyme displays full-site reactivity.
Binding of 6PG and 5-phospho-ribonate (5PR) poorly affects the dissociation constant of the
coenzymes, while binding of 4-phospho-erythronate (4PE), which is the most selective among the
studied inhibitors for the parasite enzyme compared to the mammalian sheep liver one, decreases
the dissociation constant of the coenzymes by two orders of magnitude. In a similar way the Kd of
4PE greatly decreases in the presence of the coenzymes. Results suggest that while 5PR acts as
substrate analogue, 4PE mimics the transition-state of the dehydrogenation. The stronger affinity of
4PE is interpreted on the basis of the enzyme mechanism, suggesting that the inhibitor forces the
catalytic K185 in the protonated state. pH curves of the mutants K185R and E192Q clearly show
that other residues are involved in mechanism. It has been shown that a change from an “open” to a
“closed” conformation is rate limiting. The conformational changes induced by substrate binding
appear to be related with the release of about one H+/enzyme dimer, and with a drastic reduction of
cysteine reactivity. The residues H188 and C372 are not directly involved in the substrate binding
but are very close to the active site. The mutants H188L and C372S show a linear relationship
between the residual activity and the number of H+ released and also with the cysteine reactivity.
These data suggest that H188 and C372 are involved in the transition from the “open” to the
“closed” form.
Further studies have been performed to better characterize the mechanism of 6PGDH. Kinetic
isotope effect studies on the reverse reaction, the reductive carboxylation of Ru5P to 6PG indicate
that the presence of 6PG changes the rate limiting step of the reaction. In the absence of 6PG the
rate limiting step is clearly identified in a conformational change of the enzyme-Ru5P complex. In
the presence of 6PG this step become fast, and the rate limiting step can be identified in an
isomerization of the enzyme-6PG complex according to the previously published data. These
results add new support to the alternative-site model for the mechanism of 6PGDH
Recommended from our members
The Opinion – Volume 24, No. 2, December 1981
No full textSelected Table of Contents Organizations Face Budget Cuts / Chuck Friedman; Eric Johnson Deli earns complaints -- and cash / Katy Perry Electronics shapes \u27office of the future\u27 / Steven Bell Time warp puts Lady of Peace alumna back in \u27Tea Room\u27 / Jeanne Anderson
Editorial Board
Chuck Friedman; George McCormick; Jeanne Anderson; Katy Perry; Deborah Ellis; Eileen Casalhttps://open.mitchellhamline.edu/the-opinion/1079/thumbnail.jp
The Opinion – Volume 24, No. 2, December 1981
No full textSelected Table of Contents Organizations Face Budget Cuts / Chuck Friedman; Eric Johnson Deli earns complaints -- and cash / Katy Perry Electronics shapes \u27office of the future\u27 / Steven Bell Time warp puts Lady of Peace alumna back in \u27Tea Room\u27 / Jeanne Anderson
Editorial Board
Chuck Friedman; George McCormick; Jeanne Anderson; Katy Perry; Deborah Ellis; Eileen Casalhttps://open.mitchellhamline.edu/the-opinion/1079/thumbnail.jp
Recommended from our members
The Opinion – Volume 24, No. 4, May 1982
No full textSelected Table of Contents Clinics: Hands on legal training / Katy Perry Mitchell trial team wins second in nation Crime rise prompts increased security / Jeanne Anderson Librarian resigns amidst problems / Jeanne Anderson Decision clarifies \u27aiding, abetting\u27 law / David Warg
Editorial Board
Chuck Friedman; George McCormick; Jeanne Anderson; Katy Perry; Deborah Ellis; Eileen Casalhttps://open.mitchellhamline.edu/the-opinion/1081/thumbnail.jp
- …
