1,720,960 research outputs found
The Appearance of Cocoa Clones Tolerant to Infection Phytophthora palmivora
Full text linkCocoa pod rot (PBK) is the main disease that attacks cocoa pods caused by P. palmivora. Fruit rot disease causes loss of cocoa yield. Cocoa pod rot disease caused by P. palmivora pathogen is not a good and stable control method. One strategy that can be used for control is to develop superior clones tolerant of P. palmivora. Breeding program to obtain P. palmivora tolerant cocoa clones by utilizing cocoa genetic resources derived from potential cultivated clones through detached pot test. The study aimed to obtain cocoa pods tolerant of pod rot using the detached pod test. The research was conducted at the Phytopathology Laboratory of the Department of Plant Pests and Diseases, Faculty of Agriculture, Andalas University. The tested cocoa clones consisted of 6 clones, e.g., Sca 12, TSH 858, ICS 60, GC 7, K TSH 858, and K ICS 60. Cocoa clones were collected from a cocoa plantation owned by PT. Host Sari Lubuk Basuk-Agam Regency. The observed variables related to P. palmivora tolerant cocoa clones were; incubation period, growth of cacao fruit spot area, and color of cocoa beans. The results showed that the TSH 858 and ICS 60 clones had an incubation period of 3 DAI, while the Sca 12, ICS 60, GC 7, K TSH 858, and K ICS 60 clones had an incubation period of 2 DAI. The TSH 858 clone grew 10.82 cm2.day 1, while the ICS 60, GC 7, K. TSH 858 and K. ICS 60 clones grew in spot area, respectively; 11.42, 12.95, 13.24 and 13.58 cm2.day-1. TSH 858 clone has potential as a tolerant clone to P. palmivora attack through detached pod test on fruit
Growth Response And Results Of Local Upland Rice Cultivars West Sumatera On Ultisol Land
No full textUpland rice whose cultivated on dry land, which has limited needs for growth and production. The low upland rice production is due to the lack of high yielding varieties that can adapt, especially to marginal lands such as Ultisol. There are quite many local upland rice lines, but their potential has not been tested much. This study aimed to obtain the growth and yield of local upland rice cultivars of West Sumatra on the best Ultisol soil. The study used a completely randomized design (CRD) with four replications. The treatments were four upland rice cultivars, which consisted of; Silayang (K1), Siarang (K2), Sirah (K3) and Sigudang (K4). This data observation was analyzed for variance, and if it had a significant effect, it was continued using DMRT at the 5% level. Observations were made on plant height, the maximum number of tillers, percentage of productive tillers, length of flag leaf, age of flowering, age of the first harvest, panicle length, number of grain per panicle, the weight of 1000 pithy grain seeds, and weight of pithy grain per clump. Based on the results of the study, it was concluded that upland rice cultivar Sirah had the best growth and yield on Ultisol soil. Based on the conclusion, it is recommended to use Sirah upland rice on Ultisol soil
PELATIHAN PEMBUATAN PUPUK ORGANIK CAIR DENGAN BIO AKTIVATOR DI KENAGARIAN PANCUNG TABA KECAMATAN BAYANG UTARA KABUPATEN PESISIR SELATAN
Full text linkIncreased production in agriculture affects the income and welfare of farmers. One of the factors that determine agricultural production is the availability of sufficient nutrients for plants through fertilization. Fertilizers given in addition to chemical fertilizers (inorganic) can also be given liquid organic fertilizer (POC). Liquid organic fertilizer is essential in improving soil fertility and increasing the availability of nutrients needed by plants. This service aims to make farmers understand the importance of liquid organic fertilizer and develop it independently as an alternative fertilizer by utilizing local resources. The service is carried out in socialization and training in the manufacture of liquid organic fertilizers. Liquid organic fertilizer uses local raw materials derived from animal manure and agricultural waste such as livestock manure and husks enriched with bio activators such as EM4 and Trico G. The service has shown that farmers understand the use of liquid organic fertilizers and are technically able to prepare them properly. Good. Liquid organic fertilizer of good quality can be obtained through a fermentation process for 14 days and then applied repeatedly to various cultivated plants
PENGEMBANGAN METODE PENENTUAN KELAMIN SECARA DINI BERBASIS MOLEKULER PADA TANAMAN SALAK DIOECIOUS
Full text linkSalak merupakan tanaman buah tropik yang memiliki nilai ekonomi dengan tipe pembungaan dioecious, monoecious, dan hermaprodit. Salak dioecious yang dibudidayakan membutuhkan tanaman betina dan jantan dengan rasio 4:1. Penentuan kelamin salak dioecious secara dini menjamin rasio kelamin betina dan jantan, karena pada stadia bibit sulit untuk membedakan kelamin tanaman baik secara morfologi, isozim, dan kromosom. Penentuan kelamin berbasis molekuler dengan teknik PCR-RAPD lebih akurat. Seleksi dari 305 primer dengan metode Bulked Segregant Analysis (BSA) terdapat 35 primer kandidat sebagai pembeda kelamin tanaman salak dioecious. Uji lebih lanjut secara individu dari primer
kandidat ada 3 primer yang yang konsisten menghasilkan fragmen spesifik, yakni primer OPAP-20, OPO-17, dan UBC-454. Primer OPAP-20 menghasilkan fragmen terkait kelamin jantan dengan ukuran 570 bp, OPO-17 dengan fragmen berukuran 290 bp terkait kelamin betina, dan UBC-454 menghasilkan 2 fragmen spesifik, fragmen berukuran 940 bp terkait kelamin betina dan 950 bp terkait kelamin jantan. Kloning fragmen spesifik terkait kelamin tersebut menghasilkan efisiensi transformasi sekitar 95,00%. Hasil sekuensing fragmen spesifik dihasilkan 8 pasang primer spesifik, 2 primer diantaranya dapat digunakan untuk
membedakan jenis kelamin pada tanaman salak dioecious. Primer tersebut adalah J-3(1)OPO-17 mengamplifikasi fragmen 229 bp dengan tingkat akurasi 93,33% untuk kelamin jantan, sedangkan primer B5.3 (3)UBC454 menghasilkan fragmen
berukuran 356 bp dengan tingkat akurasi 95,00% untuk tanaman salak betina.
Kata kunci : diagnosis dini, kelamin, RAPD, dioecious, sala
PENGEMBANGAN METODE PENENTUAN KELAMIN SECARA DINI BERBASIS MOLEKULER PADA TANAMAN SALAK DIOECIOUS
No full textOPTIMASI METODE ISOLASI DNA GENOM PADA TANAMAN KAPULASAN
Full text linkABSTRACTHigh quality and quantity of genomic DNA for plant kapulasan is required for analyses based on the polymorfisme chain reaction (PCR) technic. Method DNA isolation were proven to be genus or even species spesific. Objective of this study was to determine of methode with using protocol most reliably produced sufficient quantity and good quality of plant kapulasan genomic DNA. Method utilizing Doyle and Sanghai-Maroof with storge of leaf until dry, yielded higher quality and quantity of genomic DNA.Key words : DNA, Kapulasa
West Sumatra local chili genotype appearance test
Full text linkChilli is an economically valuable horticultural crops. The exploration of local chilli genetic resources is expected to provide qualitative and quantitative character information. The study aimed to test the appearance of local chilli genotypes in West Sumatra based on qualitative and quantitative characters. The research was conducted on dry land in Tiaka Village, East Payakumbuh Subdistrict, Payakumbuh City, West Sumatra Province at an altitude of 515 m above sea level. The research was carried out from December 2020 to August 2021. The research used a fully randomised complete group design (RKLT) with 4 Treatmen 6 replications. The chilli genotypes tested were; Aka, Keriting Bukittinggi, Gero and Kopay. Variable observation of qualitative characters consisted of; anthocyanin staining on hypocotyl, stem colour, stem cross-sectional shape, leaf shape, leaf colour, flower shape, flower petal colour, flower crown colour, stigma colour, young fruit colour, old fruit colour, fruit shape, seed shape and seed colour. Quantitative traits were: plant height, stem diameter, flowering age, harvest age, fruit length, fruit diameter, number of fruits per plant, fruit weight, fruit weight per plant, fruit weight per plot, fruit weight per ha. Data for quantitative characters were analysed using PKBT-STAT 3.1C statistical software. Data for the quantitative characters leaf length, leaf width and 1000 seed weight were not statistically analysed. The results of the study concluded that Aka is one of the candidates for superior varieties. Aka chilli produces a lot of fruit (166.27 fruits/plant) with fruit weight reaching 26.95 tons/ha heavier than the Kopay variety as a comparison with fruit weight reaching 22.60 tons/ha
Adaptation of local lowland rice from West Sumatra on growth, yield, and pest and disease resistance
Full text linkRice is a strategic food commodity that needs to be maintained for production stability. This can be done by testing various aspects of both productivity and resistance to pests and diseases. West Sumatra has genetic resources of paddy rice that can be developed into superior varieties. Rice paddy genotypes that have been released into varieties such as Batang Anai, Anak Daro, Bakwan, and Marapulai. But there are also many that have not been made into varieties, including Bujang Marantau, Kuriak Kusuik, Kuriak Putiah, which are still cultivated by farmers. Therefore, testing the adaptability of local paddy rice genotypes is one of the initial strategies. The experiment was conducted on paddy fields in 2 (two) locations, Tolatang Kamang and Ampek Angkek Agam Regency, West Sumatra Province. The experiment was conducted from February to June 2022. The experiment used a Randomised Block Design with 3 blocks. Treatments consisted of 5 (five) phenotypes including; Kusuik Putiah, Kuriak Kusuik, Kuriak Putiah, Ampek Bulan Putiah, and Cantik Manis. Variables observed in the field during plant growth from the vegetative, generative phase to harvest include; plant height, number of productive tillers per clump, panicle length, flowering age, number of grains per panicle, number of full grains per panicle, harvest age, weight of 1,000 grains, dry grain weight per ha, resistance to brown stem leafhopper, and bacterial leaf blight. Observational data were analyzed statistically using the STATISTIX ver 8.0 program. Brown planthopper pest attack was observed by referring to the criteria set by IRRI. The results of the study can be concluded, Kusuik Putih is one of the local paddy rice genotypes from West Sumatra that can be proposed as a candidate for new superior varieties with dry grain weight reaching 6.95 t per ha, but, bacterial leaf blight resistance is still classified as susceptible to moderately vulnerable, followed by other local rice such as Kuriak Putiah (5.92 t.ha 1), Cantik Manis (5.55 t.ha 1), Ampek Bulan Putiah (5.30 t.ha 1), and Kuriak Kusuik (4.95 t.ha 1), which are moderately resistant to brown stem leafhopper and bacterial leaf blight
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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