266 research outputs found
Contact urticaria to giraffe hair
No full textBackground: Immediate-type hypersensitivity to animal proteins is a common problem in people occupationally exposed to animals. Methods: A 19-year-old female working as a voluntary zookeeper in her off-time suffered from hives on her forearms following contact to the fur of a giraffe. For diagnostic evaluation, skin prick tests, assessment of specific serum IgE antibodies, and basophil activation tests were performed. Results: Skin prick tests with a standard series of common aeroallergens were positive for various pollens. Prick testing with native materials was positive for extracts of hair from two different giraffe subspecies in the patient, but not in control subjects. By CAP-FEIA, no specific serum IgE antibodies to dander of a large variety of animals were found in the patient. In the basophil activation test, expression of the activation marker CD63 was induced by extract of giraffe hair on the cells from the patient, but not on those from unaffected controls. Conclusions: This patient suffers from an `exotic' immediate-type contact allergy to giraffe hair. Copyright (C) 2005 S. Karger AG, Basel
Development of a Sandwich ELISA to Measure Exposure to Occupational Cow Hair Allergens
No full textBackground: Cow hair and dander are important inducers of occupational allergies in cattle-exposed farmers. To estimate allergen exposure in farming environments, a sensitive enzyme immunoassay was developed to measure cow hair allergens. Methods: A sandwich ELISA was developed using polyclonal rabbit antibodies against a mixture of hair extracts from different cattle breeds. To assess the specificity of the assay, extracts from other mammalian epithelia, mites, molds and grains were tested. To validate the new assay, cow hair allergens were measured in passive airborne dust samples from the stables and homes of farmers. Dust was collected with electrostatic dust fall collectors (EDCs). Results: The sandwich ELISA was found to be very sensitive (detection limit: 0.1 ng/ml) and highly reproducible, demonstrating intra-and interassay coefficients of variation of 4 and 10%, respectively. The assay showed no reactivity with mites, molds and grains, but some cross-reactivity with other mammalian epithelia, with the strongest reaction with goat. Using EDCs for dust sampling, high concentrations of bovine allergens were measured in cow stables (4,760-559,400 mu g/m(2)). In addition, bovine allergens were detected in all areas of cattle farmer dwellings. A large variation was found between individual samples (0.3-900 mu g/m(2)) and significantly higher values were discovered in changing rooms. Conclusion: The ELISA developed for the detection of cow hair proteins is a useful tool for allergen quantification in occupational and home environments. Based on its low detection limit, this test is sensitive enough to detect allergens in passive airborne dust. Copyright (C) 2011 S. Karger AG, Base
Implementasi Kebijakan Program Petani Mandiri Terhadap Kebermanfaatan dalam Peningkatan Kesejahteraan Ekonomi Petani di Desa Dander Kecamatan Dander Kabupaten Bojonegoro
No full textProgram Petani Mandiri (PPM) adalah bantan hibah berupa barang kepada petani untuk memberikan kesejahteraan dan kemandirian petan. Penelitian ini mengkaji tentang implementasi kebijakan Program Petani Mandiri terhadap kebermanfaatan dalam penngkatan kesejahteraan petani di Desa Dander, Kecamatan Dander, Kabupaten Bojonegoro. Teknik pengumpulan data yang digunakan adalah observasi, wawancara, dan dokumentasi. Melalui wawancara dengan 2 ornag penyuluh pertanian dan 6 orang pengurus kelompok tani di Desa Dander ditemukan beberapa permasalahan dalam implementasi bantuan hibah uang Program Petani Mandiri, yaitu komunikasi antara implementor dengan kelompok sasaran dan kurangnya kompetensi pengurus kelompok tani sehingga menghambat implementasi bantuan hibah uang Program Petani Mandiri. Selain itu baik sebelum maupun setelah Program Petani Mandiri dilaksanakan, tidak ada perubahan perilaku petani di Desa Dander yang menunjukkan ciri petani mandiri. Pemberian bantuan berupa benih dan pupuk dapat dikatakan kurang bermanfaat apabila tidak ada perubahan perilaku yang mengarah pada kemandirian petani
Ricerca, documentazione, salvaguardia, valorizzazione e gestione nell'ambito del costituendo parco paleolitico di Isernia La Pineta (Molise, Italia)
No full textGeneration YouTube: The Demolition of Politics or the Continuation of Politics with Different Means
No full textValentin Dander analysiert in seinem Beitrag die aktuelle Diskussionskultur im soziodigitalen Raum anhand der Rezeption der Videos des 26-jährigen YouTubers Rezo und konstatiert dabei eine Verschiebung in der Wahrnehmung des Politischen. Ausgehend von Rezos YouTube-Video „Die Zerstörung der CDU“ geht Dander der Frage nach jugendlichen Praktiken der politischen Informationsbeschaffung und Meinungsbildung nach. Er begreift daher YouTube als einen Ort politischer Information und Bildung Jugendlicher, die jenseits der Schriftkultur vor allem das Visuelle bedient. Hierbei wird insbesondere danach gefragt, welche Rolle Online-Videos für das politische Informationsverhalten Jugendlicher spielen, welche Differenz sich hierbei zu älteren Generationen öffnet sowie danach, inwieweit von einem generellen Wandel der politischen Sphäre gesprochen werden kann. Der Autor betont deshalb, dass Politik und Medienkultur in der Wissens- und Informationsgesellschaft des 21. Jahrhunderts in und jenseits von Generationenverhältnissen zu denken ist. Deshalb steht uns als Prosumerinnen und Prosumern eine neuartige Form der politischen Digitalisierung und der digitalen Politik vor Augen.In his contribution, Valentin Dander analyzes the current culture of discussion in the socio-digital space through the reception of videos by the 26-year-old YouTube creator Rezo, stating a shift in the perception of the political. He therefore understands YouTube as a place of political information and education for young people who, beyond the written culture, primarily serve the visual. The author therefore emphasizes that politics and media culture can be considered in the knowledge and information society of the 21st century in and beyond generational relationships. Therefore, as a prosumer, we see a new kind of political digitization and digital politics in mind
T cells stimulated by CD40L positive leukemic blasts-pulsed dendritic cells meet optimal functional requirements for adoptive T-cell therapy.
No full textAdoptive T-cell immunotherapy may provide complementary therapy for childhood B-cell precursor acute lymphoblastic leukemia (BCP-ALL). In this study, we have analyzed the functional characteristics of anti-BCP-ALL effector T cells generated by co-culturing T lymphocytes and dendritic cells (DC) from allogeneic human stem cell transplantation (HSCT) donors. After 21-day co-culture with DC pulsed with CD40L+ apoptotic BCP-ALL blasts, T cells presented with both effector and central memory phenotype, and showed high and specific cytotoxic activity against leukemic cells (average lysis=77%), mostly mediated by CD8+ T cells. Noticeably, growth of CD4 T cells was maintained (45% of total cells), which actively produced Th1 cytokines (IFN-, TNF-, IL-2), but not IL-4, IL-5 and IL-10. Anti-BCP-ALL T cells expressed CD49d and CXCR4 (implicated in the recruitment to bone marrow), and CD62L and CCR7 (involved in the migration to lymphoid organs). In accordance with this profile, T cells significantly migrated in response to the chemokines CXCL12 and CCL19. In conclusion, stimulation of T cells with CD40L+BCP-ALL cells-loaded DC not only elicited the generation of potent and specific anti-leukemic cytotoxic effectors, but also the differentiation of specific and functional Th-1 CD4 lymphocytes. These effectors are fully equipped to reach leukemia-infiltrated tissues and have characteristics to support and orchestrate the anti-tumor immune-response
Occurrence and Antimicrobial Profile of Bacterial Pathogens in Former Foodstuff Meat Products Used for Pet Diets
No full textEuropean legislation stipulates that food no longer intended for human consumption, due to commercial reasons,
manufacturing problems, or some defect, can be used in pet feed. However, the presence of former foodstuffs in pet diets could
constitute a public health issue because pets can act as reservoirs of antimicrobial resistance genes. In this study, for the first
time, biological hazards due to the presence of Escherichia coli and Salmonella in former foodstuff meat products were
evaluated. Among the 112 samples of packaged fresh meat (poultry, pork, and beef) collected from cold storage warehouses of a
mass market retailer, the overall prevalence of E. coli and Salmonella, the prevalence of strains with multidrug resistance, the
phenotypic and genotypic characteristics of strains that produce extended-spectrum β-lactamase, and the presence of biofilm
producers were assessed. A high prevalence of E. coli was observed in former foodstuffs of poultry (100%), pork (100%), and
beef (93.3%). Salmonella Derby and Salmonella Typhimurium were found in 11.5% of the poultry samples, and Salmonella
Typhimurium was found in 13.3% of the pork samples. Salmonella was not recovered from the beef samples. Multidrug
resistance was found in E. coli and Salmonella isolates from poultry, pork, and beef. Overall, 18.2% of E. coli isolates and 20%
of Salmonella isolates were resistant to various types of antibiotics with various mechanisms of action. Salmonella isolates from
pork are an important source of extended-spectrum β-lactamase production. Both E. coli and Salmonella were carriers of
antibiotic resistance marker genes (blaTEM, blaSHV, and blaCTX-M) associated with β-lactamase production in poultry and pork.
Approximately 30% of the E. coli isolates from the various types of meat were phenotypically biofilm producers, and all carried
the fimH gene. These findings indicate that the use of former foodstuff meat products in pet diets can represent a risk for public
health
Levels of horse allergen Equ c 4 in dander and saliva from ten horse breeds
No full textBackground: Horses are an important source of allergens, but the distribution of horse allergens is poorly understood. Five horse allergens have been identified, Equ c 1-4 and 6. Equ c 4 seems to be an important allergen, with an IgE-binding frequency of 77% in horse-sensitized individuals.0000 Objectives: The aim of this study was to investigate levels of horse allergen Equ c 4 in dander, saliva and urine from ten horse breeds. Method: The study population included 170 horses (87 mares, 27 stallions, 56 geldings) from ten breeds. Horse dander, saliva and urine samples were collected. Levels of horse allergen Equ c 4 were quantified using a two-site sandwich ELISA (mAb 103 and 14G4) and were expressed as Equ c 4 U/mu g protein. Results: The horse allergen Equ c 4 was present in all dander and saliva samples from ten horse breeds, with high within-breed and inter-breed variations; GM values were 639 Equ c 4 U/mu g protein (range 5-15 264) for dander and 39.5 (4-263) for saliva. Equ c 4 was found in 19/21 urine samples. Adjusted for age, sex and changes over time, no differences between breeds could be seen in dander, while in saliva the North Swedish horse showed lower levels of Equ c 4 than any other breed. The levels of Equ c 4 protein in dander and saliva were significantly higher in samples from stallions compared to mares and geldings, independent of breed. Conclusions and Clinical Relevance: The results show a high variability in allergen levels of Equ c 4 in dander and saliva both within and between breeds. Significantly higher levels were found in stallions compared to mares and geldings, independent of breed. Results suggest that none of the horse breeds studied can be recommended for individuals allergic to Equ c 4
Über den Widerstand zu coding publics
No full textVerständig D. Über den Widerstand zu coding publics. In: Dander V, Bettinger P, Ferraro E, Leineweber C, Rummler K, eds. Digitalisierung – Subjekt – Bildung. Kritische Betrachtungen der digitalen Transformation. Leverkusen-Opladen: Verlag Barbara Budrich; 2020: 136-158
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