88,907 research outputs found
Transgenic expression of <i>dSERT</i> with both “<i>TPH”-Gal4</i> and <i>TRH-Gal4</i> is sufficient to rescue increased sleep in <i>dSERT</i><sup><i>16</i></sup> mutants.
No full text(A-C) Quantification of total sleep (A), daytime sleep (B) and nighttime sleep (C) in control w1118;TPH-Gal4 (grey), w1118;TPH-Gal4, dSERT16 (red), and w1118;TPH-Gal4, dSERT16; UAS-dSERT (green) flies. (D-F) Quantification of total sleep (D), daytime sleep (E), and nighttime sleep (F) in w1118;TRH-Gal4 (grey), w1118;dSERT16; TRH-Gal4 (red), and w1118;dSERT16; TRH-Gal4/UAS-dSERT (green) flies. (G-I) Quantification of total sleep (G), daytime sleep (H), and nighttime sleep (I) in w1118; TPH-Gal4; TRH-Gal4 (grey), w1118;TPH-Gal4, dSERT16; TRH-Gal4 (red), and w1118;TPH-Gal4, dSERT16; TRH-Gal4/ UAS-dSERT (green) flies. Graphs show individual datapoints and group means ± SEM. one-way ANOVA, with Tukey post-hoc test (p≤0.0332*, p≤0.0021**, p≤0.0002***, p≤0.0001****).</p
CELL-TYPE-SPECIFIC LIMITATION ON IN VIVO SEROTONIN STORAGE FOLLOWING ECTOPIC EXPRESSION OF THE DROSOPHILA SEROTONIN TRANSPORTER, DSERT
No full textThe synaptic machinery for neurotransmitter storage is cell-type specific. Although most elements of biosynthesis and transport have been identified, it remains unclear whether additional factors may be required to maintain this specificity. The Drosophila serotonin transporter (dSERT) is normally expressed exclusively in serotonin (5-HT) neurons in the CNS. Here we examine the effects of ectopic transcriptional expression of dSERT in the Drosophila larval CNS. We find a surprising limitation on 5-HT storage following ectopic expression of dSERT and green fluorescence protein-tagged dSERT (GFP-dSERT). When dSERT transcription is driven ectopically in the CNS, 5-HT is detectable only in 5-HT, dopamine (DA), and a very limited number of additional neurons. Addition of exogenous 5-HT does not dramatically broaden neuronal storage sites, so this limitation is only partly due restricted intercellular diffusion of 5-HT. Furthermore, this limitation is not due to gross mislocalization of dSERT, because cells lacking or containing 5-HT show similar levels and subcellular distribution of GFP-dSERT protein, nor is it due to lack of the vesicular transporter, dVMAT. These data suggest that a small number of neurons selectively express factor(s) required for 5-HT storage, and potentially for function of dSERT. (c) 2006 Wiley Periodicals, Inc.X1111sciescopu
John F. Kucia interview
No full textFor 34 years Dr. John F. Kucia served Xavier University, first as the Director of Alumni Relations from 1984 to 1986, then as Assistant to the President from 1986 to 1990, and finally as Administrative Vice President from 1990 to 2018. During these years, he worked closely with five different Xavier presidents and played a key role in transforming Xavier’s campus. In addition, Kucia oversaw Xavier’s intercollegiate athletic program through a period of growth. In this interview, he discusses the five Xavier presidents with whom he worked, identifying the challenges and achievements of each. He talks about the land acquisitions, building projects, and facilities development that occurred during his tenure, including the Cintas Center, the University Station and the Health United Building. He also discusses the success of Xavier’s athletic programs and the vital role that sports play in university life. Kucia earned a doctorate in higher education management from the University of Pennsylvania and is co-author of the book Leadership in Balance: New Habits of the Mind, which he talks about in the interview. He currently serves as Xavier’s Vice President Emeritus
A saga de um clã Xavier
No full textThe book brings together historical memories of the Xavier family, of which the author is a member, and includes everything from the heroic episode between members of the Xavier family and Lampião's gang, to a Xavier family tree and tributes to the members of this family, as well as photographic records of known members. The author rescues and preserves important memories of the family, reconstructing facts that, if told orally, could gradually be erased. The story told by Maria do Socorro Cardoso Xavier is also a way of preserving the memory of Ipueira, reporting on the social life and historical setting of the place, using family narrative.O livro reúne registros de memórias históricas da família Xavier, da qual a autora é membro,
e traz desde o episódio heróico entre membros da família Xavier e o bando de Lampião, à
uma árvore genealógica dos Xavier e homenagens aos membros dessa famílias, além de
registros fotográficos dos membros conhecidos. A autora resgata e preserva memórias
importantes da família, reconstituindo fatos que, contados oralmente, poderiam se apagar aos
poucos. A história narrada por Maria do Socorro Cardoso Xavier também é uma forma de
preservar a memória de Ipueira, relatando sobre a vida social e cenário histórico do lugar,
utilizando da narrativa familiar
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Sir William Collins and Xavier Herbert
No full textSir William Collins, publisher and Xavier Herbert, author. Hand written comment about the photograph by Xavier Herbert on verso. [Gift of David Rowbotham
San Xavier District pedestrian access and safety study : final report
No full textabstract: The San Xavier District of the Tohono O’odham Nation is home to approximately 2,000 people. Numerous others come to the District to visit the San Xavier del Bac Mission Church. The San Xavier District Pedestrian Access and Safety Study is being prepared to improve the walking and bicycling environment on the San Xavier District for Community members and visitors alike. The study is being funded by the Arizona Department of Transportation Multimodal Planning Division’s Planning Assistance for Rural Areas program.Includes bibliographical references
Interview with Xavier Aldana Reyes
No full textXavier Aldana Reyes is Reader in English Literature and Film in Manchester, a founding member of the Manchester Centre for Gothic Studies, and the author of Spanish Gothic: National Identity, Collaboration and Cultural Adaptation (2017) and Gothic Cinema (2019). His publications in Gothic and horror studies include Twenty-First-Century Gothic: An Edinburgh Companion (with Maisha Wester; 2019), Horror: A Literary History (2016) and Digital Horror: Haunted Technologies, Network Panic and the Found Footage Phenomenon (with Linnie Blake; 2015). Aldana Reyes also edited fiction anthologies for the British Library series, Tales of the Weird, including the following titles: The Gothic Tales of H.P. Lovecraft (2018), The Weird Tales of William Hope Hodgson (2019), Promethean Horrors: Classic Tales of Mad Science (2019) and Roarings from Further Out: Four Weird Novellas, by Algernon Blackwood (2019)
Xavier Albó: Memoria, Crónica, Perfil
No full textAuthor historicizes the monumental contribution to the study of Quechua of linguist and anthropologist Xavier Albó. Author recalls Albó's early linguistic and anthropological influence during the late 1960s as a clear inspiration to study Quechua linguistics, the largest indigenous language spoken today in the Americas
<i>dSERT</i><sup><i>16</i></sup> mutants exhibit defects in feeding.
No full text(A) Quantification of nighttime sleep for wild-type w1118 (grey) and dSERT16 (red) over a 3-day period that began 1 day after initial loading into DAMs tubes. Baseline (Day 1): flies kept on standard food. Starvation (Day 2): flies transferred to agar for food deprivation. Recovery (Day 3): flies transferred to fresh food. (B-D) Food uptake is significantly reduced in starved dSERT16 mutants (red) compared to controls (grey). (B) Average feeding score was calculated by visual inspection of abdomen dye levels (n = 10 groups of 10 flies). (C) Representative images depict visual feeding score used to assay food uptake. (D) Quantification via spectrophotometry of ingested blue dye (n = 13 groups of 10 flies). Fly survival rate under starvation stress (E) or desiccation stress (F). dSERT mutants (red) show decreased survival under starvation stress (E) and increased survival when compared to controls (black) under desiccation stress (F) (n = 100 flies for each genotype and experiment). Graphs (A,B,D) show individual datapoints and all show group means ± SEM. A, two-way ANOVA, with Tukey post-hoc test (p≤0.0002***, p≤0.0001****; B-D: two-tailed unpaired t test (p≤0.0001****); E-F, Log-rank Mantel-Cox test, (p≤0.0021**, p≤0.0001****).</p
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