1,721,094 research outputs found
Application of Denaturing Gradient Gel ElectrophoresisApplication of Denaturing Gradient Gel Electrophoresis(DGGE) Methods on Parent-Offspring Relationship of the Coral Pocillopora damicornis
Full text linkDGGE (Denaturing Gradient Gel Electrophoresis) is the most powerful methods for mutation detection currentlyavailable. In DGGE, DNA fragments of the same length but with different sequences can be separated. Thesensitivity of DGGE to slight sequence differences is high since single base changes could be observed. Therehas been a debate about whether planulae of the coral Pocillopora damicornis are produced sexually or asexually.If produced sexually, planulae are expected to be genetically different from each other and also from theirparents. In order to detect possible genetic difference between planulae and their parents, DGGE analysis ofITS2 region of rDNA was used. If there are genetic differences, it is proved that planulae are producedsexually. A total 49 adult colonies and 78 planulae from 11 localities were used for analysis. However, only in2 families (Bise01#02 and Bise02#03YL) showed different DGGE profile, suggesting genetic difference betweenparent and offspring. The attempt to detect genetic difference in planulae of P. damicornis and their parentsusing DGGE method was not completely success since DGGE method can not prove clearly the genetic differencebetween parent and offspring. However, the possibility that DGGE method is applicable for studying coral canbe suggested. PCR-DGEE amplification may perform with new STR (short tandem repeat) polymorphic loci ofP. damicornis that currently found to answer whether planulae are produced sexually or asexually.
DGGE (Denaturant Gradient Gel Electrophoresis) merupakan salah satu teknik akurat untuk mengetahui mutasiDNA. Sensivitas DGGE sangat tinggi, karena dengan menggunakan DGGE, fragmen DNA yang memiliki panjangbasa yang sama dapat dipisahkan hanya berdasarkan perbedaan satu basa penyusunnya saja. Model reproduksikarang Pocillopora damicornis telah lama menimbulkan perdebatan. P. damicornis dilaporkan memproduksi planulaesecara seksual dan aseksual. Jika diproduksi secara seksual, planula akan berbeda secara genetik denganinduknya. Analisa DGGE dikombinasikan marker ITS2 dari rDNa digunakan untuk mendeteksi perbedaan tersebut.Jika terdapat perbedaan genetik, dapat disimpulkan planulae diproduksi secara seksual. Analisa dilakukan terhadap53 koloni dan 78 planulae dari 11 lokasi yang berbeda. Dua famili (Bise01#02 and Bise02#03YL) mempunyaiprofil DGGE berbeda antara planulae dan induknya. Hal ini menunjukkan adanya perbedaan susunan gen antaraplanulae dan induknya dan kemungkinan produksi planulae dilakukan secara seksual. Namun demikian, kombinasianalisa DGGE dan marker ITS2 dari rDNA kurang memberi hasil memuaskan. Saat ini telah ditemukan STR (shorttandem repeat) spesifik untuk P. damicornis. Kombinasi penggunaan amplifikasi PCR-DGGE dengan STR diharapkanlebih akurat untuk menjawab permasalahan produksi planulae pada karang P. damicornis
PEMBUATAN PLASTIK BIODEGRADABLE MENGGUNAKAN PATI DARI UMBI KELADI
Full text linkBIODEGRADABLE PLASTICS FROM STARCH OF COLOCASIA
ESCULENTA
(Diah Permata Sari, 2014, 42 page, 6 tabel, 8 picture, 4 attachment)
Taro (Colocasia esculenta) is a plant that has low economic value and also
underutilized because of taro (Colocasia esculenta) contains calcium oxalate
which causes GATA-itching and irritation of the skin. Therefore taro (Colocasia
esculenta) can be used as raw material for biodegradable plastics. This study was
conducted to determine the addition of sorbitol to obtain better quality plastic
(optimum conditions). In this study conducted by varying sorbitol as much as
20%, 30%, 40%, 50% and 60% of the weight of starch. The results showed that
the lower the composition of sorbitol is used then the resulting plastic is getting
hard and stiff, and conversely the more the composition of sorbitol are used then
the resulting plastic will be soft and easily crushed. Sorbitol concentration can
best be used to produce biodegradable plastics is at a concentration of 20% and
60% which has a thickness of 0.45 mm,% elongsitas of 1.9% and 0.98% and the
tensile strength test of 0.0004 MPa and 0, 0038 MPa
Keyword : Colocasia esculenta, Sorbitol, Biodegradable Plasti
Pembingkaian Berita Media Massa Nasional (Analisis Framing Pemberitaan Tiga Tahun Pemerintahan Joko Widodo Dan Jusuf Kalla Di Majalah
Full text linkPenelitian ini berjudul ―Pembingkaian Berita Media Massa Nasional
(Analisis Framing Pemberitaan Tiga Tahun Pemerintahan Joko Widodo dan Jusuf
Kalla). Penelitian ini bertujuan untuk mengetahui bagaimana majalah berita
mingguan Gatra membingkai berita terkait tiga tahun pemerintahan Joko Widodo
dan Jusuf Kalla dalam sudut pandang politik dan penegakan hukum untuk
mengetahui keberpihakan Gatra dalam memberitakannya.
Metode penelitian yang digunakan adalah metode penelitian analisis isi
kualitatif. Teori yang digunakan dalam penelitian ini adalah komunikasi massa,
media massa, produk media massa, konstruksi realitas pemberitaan dalam media
massa, teori level pengaruh media dan analisis framing. Sementara teknik analisis
yang digunakan adalah analisis framing model Robert Entman. Berita yang
menjadi objek penelitian adalah laporan utama edisi khusus tiga tahun
pemerintahan Jokowi-Jk terkait bidang politik dan penegakan hukum yang terbit
pada tanggal 19-25 Oktober 2017 yang berjumlah empat berita. Empat berita
tersebut dianalisis menggunakan analisis framing model Robert N. Entman
dengan empat perangkat unit analisis, define problem, diagnose cause, make
moral judgement, treatment recommendation.
Hasil penelitian ini menunjukkan bahwa majalah Gatra dalam membingkai
berita cenderung subjektif dan memihak pemerintah. Hal ini tampak pada narasi
pemberitaan, visual image, penyajian hasil evaluasi lembaga survey, serta
pemilihan narasumber yang kurang berimbang antara plus minus evaluasi kinerja
pemerintah terkait isu kebebasan berpendapat, SARA, dan konsensus nasional
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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