554 research outputs found
CRP-cAMP represses <i>hilA</i> expression in mid-logarithmic growing cells.
No full text(A) Transcriptional expression of hilA in a wild-type (WT) and a Δcrp derivative strain. β-galactosidase activity from a hilA-lacZ fusion was assessed in LB cultures grown at 37°C up to either mid-logarithmic (OD600nm 0.4) or early stationary (OD600nm 2.0) phase of growth. Data from three independent experiments are averaged and the standard deviation is shown. **, ppcrp derivative strains, grown as in A. (C). Immuno-detection of HilA-3xFLAG on whole cell extracts from cultures of the WT and Δcya derivative strains in the absence (-) or presence (+) of cAMP (5 mM). Cultures were grown in LB at 37°C up to an OD600nm of 0.4. (D) Effect of over-expressing the cAMP-phosphodiesterase CpdA. Immunodetection of HilA-3xFLAG was performed on cultures of the WT and a Δcya derivative strain carrying either pTrc99a (-, control vector) or pCpdA (+, pTrc99a+cpdA). Cultures were grown as in C in LB supplemented with IPTG (0.1 mM). In B, C and D the relative amount of HilA-3xFLAG is indicated. In each case the reference value was set as one. Coomassie Blue staining of the whole cell extracts serve as loading controls. Full length images of the Western blots, including molecular mass markers, are shown in S12 Fig.</p
PENGEMBANGAN MODUL PEMBELAJARAN TEMATIK BERBASIS KEARIFAN LOKAL MBOJO TENTANG LA HILA DONGGO PADA KELAS IV UNTUK MENINGKATKAN HASIL BELAJAR
No full textModel pengembangan R&D digunakan dalam penelitian ini referensi Borg and Gall untuk membuat produk pembelajaran berupa modulpemebelajaran tematik berabasis kearifan lokal mbojo tentang sejarah Lahila Donggo pada tema 8 pembelajaran tematik. Penelitian ini bertujuan untuk (1) Mengetahui kevalidan pengembangan Modul pembelajaran tematik berbasis kearifan lokal mbojo tantang sejara La Hila Donggo untuk meningkatkan hasil belajar siswa kelas IV SD. (2) Mengetahui kepraktisan pengembangan Modul pembelajaran tematik berbasis kearifan lokal mbojo tantang sejara La Hila Donggo untuk meningkatkan hasil belajar siswa kelas IV SD. (3) Mengatahui keefektifan pengembangan Modul pembelajaran tematik berbasis kearifan lokal mbojo tantang sejara La Hila Donggo untuk meningkatkan hasil belajar siswa kelas IV SD
Hha Is a Negative Modulator of Transcription of <i>hilA</i> , the <i>Salmonella enterica</i> Serovar Typhimurium Invasion Gene Transcriptional Activator
No full textABSTRACT
An early step in the establishment of
Salmonella enterica
serovar Typhimurium murine infection is the penetration of the intestinal mucosa of the small intestine. The majority of the genes responsible for the
Salmonella
invasive phenotype are encoded on
Salmonella
pathogenicity island 1, and their transcription is controlled by the
hilA
transcriptional activator. The expression of
hilA
is regulated by environmental signals including oxygen, osmolarity, pH, and growth phase such that the presence of any one suboptimal condition results in repression of
hilA
expression and the invasive phenotype. We have conducted a search for negative regulators of
hilA
by introduction of a
Salmonella enterica
serovar Typhimurium chromosomal DNA gene bank into a
Salmonella enterica
serovar Typhimurium
hilA
::Tn
5lacZY
reporter strain. This screen has identified the
hha
gene as a regulator that exerts a negative influence on
hilA
expression. Plasmid-encoded
hha
significantly reduces
hilA
::Tn
5lacZY
chromosomal expression, as well as expression of the invasion genes
invF
,
prgH
, and
sipC
. An
hha
null mutation results in substantial derepression of both chromosomally encoded and plasmid-encoded
hilA
::Tn
5lacZY
expression. Introduction of plasmid-encoded
hha
into strain SL1344 results in attenuation of invasion using in vitro and in vivo assays. Importantly, purified Hha protein was found to bind to a
hilA
DNA promoter fragment, suggesting that the regulatory activity of the Hha protein occurs at the
hilA
promoter. These data add detail to the developing model of the regulation of
Salmonella
invasion genes.
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Screening the Author: The Literary Biopic
No full textThis book is the first comprehensive analysis of the contemporary representation of the author on screen. It does this through two main approaches: by looking at how biographies of well-known authors in Western culture have been adapted onto the film and television screen; and by examining the wider preoccupation with the idea of what the ‘author persona’ means in broader economic, cultural, industrial, and ideological terms. Drawing from current debates about the uses of the heritage industry and conventions of the Hollywood biopic and celebrity culture, this book re-frames the analysis of the author on screen in contemporary culture and theorises it under its own unique genre: the ‘literary biopic’. With case studies including adaptations of the biographies and cultural personas of William Shakespeare, Jane Austen, Oscar Wilde, Sylvia Plath, Virginia Woolf, and Allen Ginsberg—to name a few–this book examines how and why the author continues to be a prominent screen and cultural preoccupation
Analisis Campur Kode Oleh Tokoh-tokoh Dalam Film La Hila Donggo Karya Ary Ipan
No full textCampur kode dalam film tentu menarik untuk dikaji. Salah satunya film La Hila Donggo yang berasal dari Bima, diangkat dari cerita daerah setempat dengan memadukan unsur bahasa Bima dengan bahasa Indonesia serta memiliki nilai-nilai yang patut dipelajari. Penelitian ini bertujuan untuk mengetahui bentuk campur kode dalam film La Hila Donggo serta faktor penyebab terjadinya campur kode. Penulisan artikel ini menggunakan jenis penelitian deskriptif kualitatif dengan metode penelitian yang digunakan yaitu metode simak dan menggunakan teknik catat untuk memperoleh data lisan. Adapun analisis data menggunakan metode padan dengan menggunakan teknik hubung banding menyamakan. Langkah-langkah dalam analisis data yaitu, menampilkan data bentuk campur kode, menganalisis bentuk campur kode, mengklasifikasikan bentuk campur kode, menyamakan bentuk campur kode yang sesuai dengan artinya, dan menarik kesimpulan bentuk campur kode. Adapun hasil penelitian ini ditemukan 28 bentuk campur kode ke dalam mencakup kata 17 data seperti, ori,santabe, ina, nami, nahu, mada, nggomi, ompu. Kemudian frasa 7 data seperti, dou Donggo, dana Mbojo, ana mone, lenga mada. Selanjutnya, klausa 4 data seperti, hademu nahu lenga, lao nggomi, nahu eda, lembo ade ana. Selanjutnya, adapun faktor yang menyebabkan terjadinya campur kode yang terjadi dalam film La Hila Donggo meliputi, (a) faktor kebahasaan, (b) faktor kebiasaan, dan (c) faktor sikap penutur
Characterization of the SPI-1 and Rsp type three secretion systems in Pseudomonas fluorescens F113
No full textPseudomonas fluorescens F113 is a plant growthpromoting
rhizobacterium (PGPR) isolated from the
sugar beet rhizosphere. The recent annotation of the
F113 genome sequence has revealed that this strain
encodes a wide array of secretion systems, including
two complete type three secretion systems (T3SSs)
belonging to the Hrp1 and SPI-1 families. While Hrp1
T3SSs are frequently encoded in other P. fluorescens
strains, the presence of a SPI-1 T3SS in a plantbeneficial
bacterial strain was unexpected. In this
work, the genetic organization and expression of
these two T3SS loci have been analysed by a combination
of transcriptional reporter fusions and
transcriptome analyses. Overexpression of two transcriptional
activators has shown a number of genes
encoding putative T3 effectors. In addition, the influence
of these two T3SSs during the interaction of
P. fluorescens F113 with some bacterial predators
was also assessed. Our data revealed that the transcriptional
activator hilA is induced by amoeba and
that the SPI-1 T3SS could potentially be involved in
resistance to amoeboid grazing
Modified Open Circuit and Vacuum-assisted Venous Return Reduces Blood Usage During Cardiopulmonary Bypass
No full textObjectives: To determine whether vacuum-assisted venous return has clinical advantages over conventional gravity drainage apart from allowing the use of smaller cannulas, shorter tubing and reduced priming.
Methods: A total of 80 CABG operations were performed at our institution
between July 1999 to December 2010, using vacuum-assisted venous return
with small venous cannulas connected to short tubing. These were randomized
with 80 CABG operations using conventional gravity drainage. Priming volume,
hematocrit value, red blood cell usage, and total blood product usage were
compared by means of multivariate analysis.
Results: The priming volume was 780+/-140mL for small-cannula vacuumassisted
venous return, 1300+/-88mL for gravity drainage (P <.0001). Smaller
priming resulted in higher hematocrit values both at the beginning of cardiopulmonary
bypass (26%+/-5% compared with 21%+/-4%, respectively, P <.0001)
and at the end (28%+/-4% compared with 24%+/-4%, respectively, P <.0001).
Red cell transfusions were used in 12% of the patients having small-cannula
vacuum-assisted venous return and 41% of the patients having gravity drainage
(P =.001); total blood product usage was 15% and 61%, respectively (P =.001).
Despite a postoperative blood loss, length of stay in intensive care unit was
similar in both groups; the association of vacuum-assisted venous return with
lowered blood product usage was confirmed also in the postoperative period.
Conclusions: Modified open circuit and vacuum-assisted venous return result in 1) higher hematocrit values during cardiopulmonary bypass and 2) decreased red cell and total blood product usage
Penelitian kondisi terumbu karang telah dilakukan pada bulan Januari, Juni dan Juli lg94 di Perairan Hila dan Morela (Pulau Ambon) dan Bagian Tenggara Pulau Batanta, Sorong, Irian Jaya. Penelitian ini menggunakan metode Manta Tow Suruey d,an Line IKONDISI TERUMBU KARANG DI PERAIRAN HILA DAN MORELA (PULAU AMBON) SERTA BAGIAN TENGGARA PULAU BATANTA (SORONG)
No full textPenelitian kondisi terumbu karang telah dilakukan pada bulan Januari, Juni dan Juli 1994 di Perairan Hila dan Morela (Pulau Ambon) dan Bagian Tenggara Pulau Batanta, Sorong, Irian Jaya. Penelitian ini menggunakan metode Manta Tow Suruey d,an Line Intercept Tiansect. Pengolahan data karang menggunakan software dBase III, dan program Lifeform</jats:p
Transplantation Pneumonia
No full textA clinically distinct pneumonitis occurred in six renal transplant recipients receiving azathioprine and prednisone immunosuppressive therapy. The patients ranged in age from 3 to 20 years. The onset was 42 to 102 days postoperatively, coinciding with decrease in prednisone dosage below approximately 1 mg per kilogram of body weight per day. Mild nonproductive cough, fever, and cynanosis were present. Chest x-rays demonstrated extensive hazy to nodular infiltrates usually involving both hila and lower lung fields. Cold agglutinins were present in five patients. Pulmonary function studies demonstrated an alveolar capillary block. The duration of illness was 12 to 34 days and was not influenced by antibiotic therapy. Autopsy of the single case in which death occurred revealed Pneumocystis carinii pneumonia and disseminated cytomegalic inclusion-body disease. © 1964, American Medical Association. All rights reserved
Queer Israelis in Berlin:Belonging, Resistance, and the Politics of Emigration
No full textNathan: ‘My parents still call me, even today, asking “so, what about a boyfriend?” I tell them, “listen, I have a boyfriend every day! Every day I have a new boyfriend”. And then the question is of course, “do you want to be alone?” And this is another reason why it is better to live in this country [Germany], where being alone is not the Mark of Cain.’ Sexual orientation and left-wing political affiliation often play significant roles in decisions to leave Israel. Queer Israelis question nationalist and heteronormative expectations regarding army service, monogamy, and procreation. A general despair over the current political situation and the collapse of democracy further motivate their departures and leave many feeling that they don’t belong to the Israeli national collective, while Berlin is frequently celebrated as a cosmopolitan and unassuming city where LGTB people can live in peace. Hila Amit argues that queer Israeli emigrants, in their decision to depart, undermine Zionist ideology as well as change the obvious paths of resistance to Zionism. In stepping out of the territory of Israel, they avoid the Zionist demand to perform as strong, masculine courageous citizens. But the left-wing resistance to the regime is just as committed to a heroic ideal – the willingness to take part in often violent demonstrations and risk imprisonment or injury. Amit recognizes the vulnerability of the emigrants, who can no longer bear the hardship of the life offered to them in Israel. To be explored, then, is the political potential of passivity and unheroic conduct. As Adi, one of the participants of the study, puts it: ‘I’ve been an activist for 10 years. I can’t live there anymore. People come to Berlin to heal, that’s why some people call it the Berlin Sanatorium.’ The discussion builds on the contributions to A Queer Way Out: The Politics of Israeli Emigration (2018), an award-winning study by Hila Amit. The evening presents an opportunity to hear both from the author and the participants of the study and hence the rare chance to discuss the product of a long-term research project with the subjects of the research themselves.
Hila Amit is a writer, independent scholar, and Hebrew teacher. Her research focuses on political activism, queer kinship, national belonging, and diasporic communities. Ruth Preser teaches at the University of Haifa and is a co-founder of the Haifa Feminist Institute. She is a former ICI Fellow. Yossi Bartal was born and raised in Jerusalem and has been living in Berlin for 11 years. He studies musicology and gender studies and writes extensively in Hebrew, German, and English about politics, music, and culture. Gil Kremmer was born and raised in Israel. She fell in love with Berlin during a backpacking trip through Europe. Works as a tour guide and as a DJ and lives with her wife and young kid in Berlin
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