14,517 research outputs found
Positive hits from the SGA screening
No full textdataset posted on 2025-04-01, 19:51 authored by Ana Amiama-Roig, Marta Barrientos-Moreno, Esther Cruz-Zambrano, Luz M. López-Ruiz, Román González-Prieto, Gabriel Ríos-Orelogio, Félix Prado
The name of the positive hits, their PCR validation and the effect of the null mutant on RFA1-MN viability are indicatedPeer reviewe
Saccharomyces cerevisiae genes studied in the SGA screening
No full textdataset posted on 2025-04-01, 19:51 authored by Ana Amiama-Roig, Marta Barrientos-Moreno, Esther Cruz-Zambrano, Luz M. López-Ruiz, Román González-Prieto, Gabriel Ríos-Orelogio, Félix Prado
Genes analyzed in the customized library of null mutants are shown.Peer reviewe
Raw data for figure panels
No full textfigure posted on 2025-04-01, 19:51 authored by Ana Amiama-Roig, Marta Barrientos-Moreno, Esther Cruz-Zambrano, Luz M. López-Ruiz, Román González-Prieto, Gabriel Ríos-Orelogio, Félix Prado
Original blots for the indicated figure panels are shown.Peer reviewe
O Aluguel Social como Expressão do Déficit Habitacional no Maciço do Morro da Cruz – Florianópolis/SC: Desafios ao Serviço Social
No full textTCC (Graduação) - Universidade Federal de Santa Catarina. Centro Socioeconômico. Serviço Social.O presente trabalho pretendeu analisar o déficit habitacional no Maciço do Morro da Cruz e sua expressão através do benefício eventual Aluguel Social, destacando uma reflexão sobre os desafios colocados ao Serviço Social nessa realidade. A pesquisa, de abordagem quantiqualitativa, teve como campo de coleta de dados os 56 beneficiários do Aluguel Social que aguardam por unidades habitacionais pelo Projeto Maciço do Morro da Cruz/PAC. Os resultados do estudo se somam ao debate social e acadêmico acerca da questão social do déficit habitacional. Os dados produzidos poderão contribuir para a discussão acerca da problemática do déficit habitacional, bem como levantar o debate acerca dos cidadãos que são marginalizados pela política habitacional e o papel do Serviço Social no sentido de promover ações que assegurem o direito à moradia. Nesse sentido, a pesquisa contribuiu para compreender a realidade social e pensar as políticas sociais com vistas à ampliação do espaço de atuação do profissional de Serviço Social neste recorte das políticas públicas e de seus desafios e possibilidades no enfrentamento das expressões da questão social. Acredita-se, portanto, que para se estimular a diminuição do déficit habitacional em Florianópolis, seja necessária a articulação entre as três esferas do poder público, aliados ao controle de novas ocupações irregulares, promoção da regularização urbanística e fundiária nas ZEIS e fomento a produção de novas habitações populares acessíveis para a população de baixa renda, visando mitigar a reprodução da exclusão territorial. No bojo da atuação do Serviço Social no PMMC/PAC, o presente trabalho destacou como sendo fundamental a discussão permanente com as bases da sociedade representativa, assim como incentivo a participação democrática nas decisões do poder público
Viajantes ex-cêntricas nas histórias de Ana Miranda
No full textTese (doutorado) - Universidade Federal de Santa Catarina, Centro de Comunicação e Expressão. Programa de Pós-Graduação em Literatura.No conjunto das narrativas ficcionais da escritora brasileira Ana Miranda, a temática da viagem # considerada uma das mais férteis da literatura ocidental de todos os tempos # ocupa um espaço de centralidade, podendo até mesmo ser vista como o mais importante eixo de estruturação de suas obras. O estudo realizado pela presente tese tem por objetivo analisar o tratamento dado ao tema da viagem nos romances Desmundo, O retrato do rei, Dias & Dias e Amrik, evidenciando que por intermédio das narradora-viajantes # Oribela, Mariana, Feliciana e Amina # a autora promove um diálogo entre diferentes culturas, gêneros, etnias e gerações, ao mesmo tempo em que estabelece um profícuo diálogo com o passado em sua invariante problematização concernente aos limites e cruzamentos entre o discurso ficcional e os discursos narrativos extraliterários que o cercam, sobretudo o histórico e o biográfico. In the whole of Brazilian writer Ana Miranda#s fictional narrative, the travel thematic # considered one of the most fertile themes of the western literature of all times # occupies a central space, and can even been seen as the most important structuring axis of Miranda#s works. The aim of the present thesis is to analyze the treatment given to the travel subject in the novels Desmundo, O retrato do rei, Dias & Dias and Amrik, emphasizing that, through the traveling narrators, Oribela, Mariana, Feliciana and Amina, the author promotes a connection between different cultures, genders, ethnics and generations, at the same time that establishes a profitable dialogue with the past in her invariant problematization concerning the limits and crossings between the fictional and the extra-literary narrative discourses that surrounds it, mainly the historical and the biographic ones
Additional genetic requirements for RFA1-MN cell viability in the absence and presence of calcium
No full textfigure posted on 2025-04-01, 19:51 authored by Ana Amiama-Roig, Marta Barrientos-Moreno, Esther Cruz-Zambrano, Luz M. López-Ruiz, Román González-Prieto, Gabriel Ríos-Orelogio, Félix Prado
(A–D) Effect of the indicated mutations in the growth of RFA1-MN cells as determined by spotting 10-fold serial dilutions of the same number of mid-log growing cells onto SMM medium without or with the indicated concentrations of CaCl2. The analyses were repeated at least twice with similar results. Mutants scored in the SGA screening are shown in bold.Peer reviewe
Differential stability of 2'F-ANA*RNA and ANA*RNA hybrid duplexes: roles of structure, pseudohydrogen bonding, hydration, ion uptake and flexibility
No full text14 pags., 7 figs., 3 tabs.Hybrids of RNA with arabinonucleic acids 2′F-ANA and ANA have very similar structures but strikingly different thermal stabilities. We now present a thorough study combining NMR and other biophysical methods together with state-of-the-art theoretical calculations on a fully modified 10-mer hybrid duplex. Comparison between the solution structure of 2′F-ANA•RNA and ANA•RNA hybrids indicates that the increased binding affinity of 2′F-ANA is related to several subtle differences, most importantly a favorable pseudohydrogen bond (2′F-purine H8) which contrasts with unfavorable 2′-OH-nucleobase steric interactions in the case of ANA. While both 2′F-ANA and ANA strands maintained conformations in the southern/eastern sugar pucker range, the 2′F-ANA strand's structure was more compatible with the A-like structure of a hybrid duplex. No dramatic differences are found in terms of relative hydration for the two hybrids, but the ANA•RNA duplex showed lower uptake of counterions than its 2′F-ANA•RNA counterpart. Finally, while the two hybrid duplexes are of similar rigidities, 2′F-ANA single strands may be more suitably preorganized for duplex formation. Thus the dramatically increased stability of 2′F-ANA•RNA and ANA•RNA duplexes is caused by differencesin at least four areas, of which structure and pseudohydrogen bonding are the most important. © The Author(s) 2010. Published by Oxford University Press.Spanish Ministerio de Ciencia e Innovacion (grants
CTQ2007-68014-C02-02 to CG and BIO2009-10964 to
MO); Fundacion Marcelino Botin (grant to MO);
Canadian Institutes for Health Research (grant to
M.J.D.); Natural Sciences and Engineering Research
Council of Canada (postgraduate scholarship to
J.K.W.). Funding for open access charge: Canadian
Institutes for Health Research
HR and chromatin assembly requirements for RFA1-MN cell viability in the absence and presence of calcium
No full textfigure posted on 2025-04-01, 19:51 authored by Ana Amiama-Roig, Marta Barrientos-Moreno, Esther Cruz-Zambrano, Luz M. López-Ruiz, Román González-Prieto, Gabriel Ríos-Orelogio, Félix Prado
(A, C, and D) Effect of the indicated mutations in the growth of RFA1-MN cells as determined by spotting 10-fold serial dilutions of the same number of mid-log growing cells onto SMM medium without or with the indicated concentrations of CaCl2. (B) DSB sensitivity of rtt105∆ cells to HO-induced DSBs, as determined by spotting 10-fold serial dilutions of the same number of mid-log growing cells. Cells were transformed with pGAL-HO and grown in glucose (GAL1p repression) and galactose-containing medium (GAL1p activation). Wild-type and rad52∆ cells were included as control. The analyses were repeated at least twice with similar results. Mutants scored in the SGA screening are shown in bold.Peer reviewe
Checkpoint activation facilitates the repair of broken forks
No full textfigure posted on 2025-04-01, 19:51 authored by Ana Amiama-Roig, Marta Barrientos-Moreno, Esther Cruz-Zambrano, Luz M. López-Ruiz, Román González-Prieto, Gabriel Ríos-Orelogio, Félix Prado
(A) Additive effect of dun1∆ and HU in the growth of RFA1-MN cells as determined by spotting 10-fold serial dilutions of the same number of mid-log growing cells onto SMM medium without or with the indicated concentrations of CaCl2 and HU. (B) Cell cycle progression and budding index of wild-type cells synchronised in G1 with α-factor and released into S phase in the presence of 400mM CaCl2. The mean and standard deviation of three independent experiments are shown. (C) Rad53 activation in wild-type cells treated or not with 0.005% MMS for 2 hours in the absence and presence of 400mM CaCl2.Peer reviewe
Reseña de la obra: “Rodríguez Cruz, A M.(1990) Historia de la Universidad de Salamanca.”
No full text[EN]Comment of the book: “Rodríguez Cruz, A M.(1990) Historia de la Universidad de Salamanca. Salamanca: Fundación Ramón Areces-Congregación de Santo Domingo.”[ES]Comentario del libro: “Rodríguez Cruz, A M.(1990) Historia de la Universidad de Salamanca. Salamanca: Fundación Ramón Areces-Congregación de Santo Domingo.
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