203,475 research outputs found
Protective role of the dynamin inhibitor Dynasore against the cholesterol-dependent cytolysin of Trueperella pyogenes
No full textThe virulence of many Gram-positive bacteria depends on cholesterol-dependent cytolysins (CDCs), which form pores in eukaryotic cell plasma membranes. Pyolysin (PLO) from Trueperella pyogenes provided a unique opportunity to explore cellular responses to CDCs because it does not require thiol activation. Sublytic concentrations of PLO stimulated phosphorylation of MAPK ERK and p38 in primary stromal cells, and induced autophagy as determined by protein light-chain 3B cleavage. Although, inhibitors of MAPK or autophagy did not affect PLO-induced cytolysis. However, 10 μM 3-hydroxynaphthalene-2-carboxylic acid-(3,4-dihydroxybenzylidene)-hydrazide (Dynasore), a dynamin guanosine 5′-triphosphatase inhibitor, protected stromal cells against PLO-induced cytolysis as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (85 ± 17% versus 50 ± 9% cell viability), measuring extracellular ATP, and kinetic assays. This was a generalized mechanism because Dynasore also protected HeLa cells against streptolysin O. Furthermore, the effect was reversible, with stromal cell sensitivity to PLO restored within 30 minutes of Dynasore removal. The protective effect of Dynasore was not conferred by dynamin inhibition, induction of ERK phosphorylation, or Dynasore binding to PLO. Rather, Dynasore reduced cellular cholesterol and disrupted plasma membrane lipid rafts, similar to positive control methyl-β-cyclodextrin. Dynasore is a tractable tool to explore the complexity of cholesterol homeostasis in eukaryotic cells and to develop strategies to counter CDCs.—Preta, G., Lotti, V., Cronin, J. G., and Sheldon, I. M. Protective role of the dynamin inhibitor Dynasore against the cholesterol-dependent cytolysin of Trueperella pyogenes
Robert M. Augros et George N. Stanciu, The New Story of Science
No full textCronin John T. Robert M. Augros et George N. Stanciu, The New Story of Science. In: Revue Philosophique de Louvain. Quatrième série, tome 84, n°63, 1986. pp. 412-415
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
La tour de BaBulles : traduction, proximité et distance dans l’Europe contemporaine
No full textC’est par cette intervention, consacrée au statut du traducteur littéraire dans le contexte actuel, que Michael Cronin a ouvert la deuxième journée du Forum sur la traduction littéraire. Selon M. Cronin, cinq grands défis se posent au traducteur pour l’avenir : l’Europe (institutionnelle), la société, l’informatique, l’éducation et les médias. 1. Europe institutionnelle Cronin part du constat que la mise en place de l’Europe se fait sous forme d’intégration économique au sein de l’Espace é..
RRS "Charles Darwin" Cruise CD166, 29 Oct - 22 Nov 2004. Sedimentary processes and deposits in the Agadir Basin and Gulf of Cadiz
No full textThe primary aim of CD166 was to undertake intensive coring in the Agadir Basin on the Northwest African margin, with the intention of characterising deep-water gravity flow processes and deposits at a basin-wide scale. In addition, it was hoped that sampling of volcaniclastic turbidites derived from Canary Islands landslides would provide insights into landslide processes and aid assessment of potential tsunami hazards. In addition, the first two days of the cruise were assigned to piston coring of a small, deep-water sand lobe in the Gulf of Cadiz, off southern Spain.In the event, CD166 proved to be a highly successful cruise. In the Agadir work area a total of 50 piston cores was collected, up to 8 m in length. Most of the cores successfully penetrated through the target turbidite units 1-14, providing sedimentological and stratigraphical data for the last 200,000 years. The piston coring mechanism was extremely reliable throughout this extensive coring effort. In the Speculobe work area, a total of 11 piston cores was collected, up to 7.7 m in length. The seabed environment in this region was highly challenging for coring, although several of the cores did recover crucial sediment sequences. The insights gained from these two datasets are likely to substantially alter current thinking on large-scale deep-water gravity flows.Wildlife observations were dominated by a variety of disoriented migrant landbirds and even a Migratory Locust arrived on board. Of scientific interest was the unusually large number of Leach’s Storm-petrels Oceanodroma leucorhoa recovered on the deck of the ship at night
Representer l'exil : le sujet. Rencontre du 12 décembre. M. Cronin, M. Gad Wolkowicz, M. Goldschmit
No full textSombrero de Freud, ViennaMarjorie Apel (14 mai 2010)Wikimedia Représenter l'exil : le sujet avec Marc Goldschmit, Michel Gad Wolkowicz et Michael Cronin Attention ! la rencontre aura exceptionnellement lieu en salle A , niveau – 1, FMSH, de 16h à 18h, Intervenants: Michael CRONIN est titulaire de la Chaire de Traductologie à la Dublin City University, Irlande. Il est l'auteur de nombreux ouvrages parmi lesquels : Translating Ireland: Translation, Languages and Identity (Cork University Pr..
La tour de BaBulles : traduction, proximité et distance dans l’Europe contemporaine
No full textC’est par cette intervention, consacrée au statut du traducteur littéraire dans le contexte actuel, que Michael Cronin a ouvert la deuxième journée du Forum sur la traduction littéraire. Selon M. Cronin, cinq grands défis se posent au traducteur pour l’avenir : l’Europe (institutionnelle), la société, l’informatique, l’éducation et les médias. 1. Europe institutionnelle Cronin part du constat que la mise en place de l’Europe se fait sous forme d’intégration économique au sein de l’Espace é..
US should help Vietnam counter China's coercion
No full textFor more about the East-West Center, see http://www.eastwestcenter.org/Patrick M. Cronin, Senior Director of the Asia-Pacific Security Program at the Center for a New American Security, explains that "China's tailored coercion is aimed not just at its neighbors but also at the United States.
Projecting the nation through sport and culture: Ireland, Aonach Tailteann and the Irish free state, 1924-32
No full textCronin was the guest editor for this issue of the journal
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