21 research outputs found
Città e territorio nel Basso Medioevo.L'esempio di Ostuni nell'età di Carlo I d'Angiò,
No full textOstuni nel Medioevo lo sviluppo urbano dall'XI alla metà del XIII secolo ; le pergamene più antiche dell'Archivio Capitolare di Ostuni (1137 - 1241)
No full textOstuni nel Medioevo lo sviluppo urbano dall'XI alla metà del XIII secolo ; le pergamene più antiche dell'Archivio Capitolare di Ostuni (1137 - 1241)
No full textFamily Secrets: When the Horror in the House Has No Obvious Cause and the Need for Unveiling
No full textAfter attending this presentation, attendees will understand the importance of both the criminological and
anthropological investigation in understanding a crime associated with forensic investigations and forensic
psychiatric and psychological investigations.
This presentation will impact the forensic science community by serving as an example of the importance of
reconstructing the life experiences of the perpetrator and the victim, in order to unveil the pathway leading to a
particularly complex murder.
Particularly violent and brutal homicides, due to their mode of expression, sometimes present a contradictory
and paradoxical scenario, complicating the forensic, psychiatric, and psychological forensic reconstructions.1
The case is that of a 67-year-old man, a typographer, married with children, whose matrimonial life was
apparently marked by quiet harmony until members of the wife’s family (sister and mother) intervened in the
relationship. After that, the man said his wife had become authoritarian and aggressive, deliberately humiliating
him, and their relationship had deteriorated. On the day of the homicide, the woman had, as usual, adopted an
authoritarian attitude and started to shout and push her husband. The husband, after having endured this humiliation
for many years, suddenly and repeatedly struck her with an axe he was carrying in a bag, inflicting numerous blows
that caused significant damage to her head. Immediately after, the man phoned for help.
The man’s psychiatric history was negative, and there were no signs of cognitive impairment. For this destructive
act directed against his wife, qualifying as abnormal on the psychopathological plane, relevant motivational elements
emerged. There were no signs suggesting a temporary psychotic decompensation nor a criminal plan. The scenario
depicted a murder of impulse rather than premeditated aggression, bearing in mind the impromptu weapon used
(he had not even removed the axe from the bag). In short, it was not an idea that had long been brooded upon and
finally implemented (as occurs in cases of delirium), but a deep underlying discomfort that finally erupted as a
result of a quarrel (although this was denied), triggered by a contingent event (a verbal provocation) that brought
on frustration, followed by an act of physical aggression (a shove) that had an explosive effect on the perpetrator
of the murder. From the medical-legal consultation, it emerged that the man had continued relentlessly striking the
woman even after she had fallen to the ground, denoting not only that his emotions were completely out of control,
but also his wish to “destroy” the victim. The homicidal behavior appeared disorganized, whereas immediately
after the murder, he reacted in a usual way, calling for help.
Faced with such a terrible scene, limited information was gained from the forensic psychiatric and psychological
assessment conducted on the perpetrator. The man had no criminal record and did not use drugs or alcohol. This
elderly person, retired, leading an ordinary and peaceful life, was described by everyone (family, friends, coworkers,
jail staff) as a kind person, very polite, and helpful. The only significant finding from information obtained from his
children was that the victim had a difficult character, was rigid and complex, and often abused her husband, who had
patiently endured it for 26 years. Despite the absence of signs of severe mental illness or important criminal motive,
the cause of this murder conducted within the domestic walls of a normal family is to be traced to the relational and
historical aspects of the family structure and of the subjects involved in the crime.2
The medicolegal, psychiatric, and forensic psychology assessments must include a criminological and relational
anthropological analysis, with the goal of establishing a reason for committing such a terrible murder, through close
study of the experiences of the subjects involved, and the context in which the murder was committed.3
1168 *Presenting Author
Reference(s):
1. Coccaro E.F., Sripada C.S., Yanowitch R.N., Phan K.L. (2011). Corticolimbic function in impulsive
aggressive behavior. Biol Psychiatry. 69:1153-1159.
2. Fornari U. (2013). Trattato di Psichiatria Forense, V ed. Utet, Torino.
3. Osumi T., Nakao T., Kasuya Y., Shinoda J., Yamada J., Ohira H. (2012). Amygdala dysfunction attenuates
frustration-induced aggression in psychopathic individuals in a non-criminal population. Journal of
Affective Disorders. 142:331-338
Synthetic and natural compounds regulate activity of ABCC6 transporter
No full textSIB 2019
Synthetic and natural compounds regulate activity of ABCC6 transporter
DEPARTMENT OF SCIENCES- UNIVERSITY OF BASILICATA, Potenza, Italy
*CORRESPONDING AUTHOR: [email protected]
ATP-binding cassette transporters (ABC transporters) are members of a superfamily consisting of 49 proteins that transport a wide variety of substrates across extra- and intracellular membranes by using energy released from ATP hydrolysis [1]. Mutations in ABC genes underlie at least 19 human genetic disorders [2]. Many transporters are involved in the multidrug resistance, that is the failure due to the reduction of cellular accumulation of drugs. Many ABC transporters inhibitors have been proposed as molecules able to reverse multidrug resistance in cancer cells [3]. ABCC6 is a member of ABCC family poorly involved in drug resistance whose mutations are associated to the Pseudoxanthoma elasticum (PXE), an autosomal recessive disease characterized by a progressive ectopic calcification [4]. Although the substrate transported is not known, ABCC6 has been shown to promote ATP release [5]. In this study we evaluate the effects of both probenecid, an uricosuric drug and quercetin, a nonterpenoid secondary metabolite on HepG2 cells. Both are known broad spectrum inhibitors of ABC transporters. The observed effects are all reversed by the addition of ATP suggesting the involvement of the ABCC6 protein.
FUNDED BY INBIOMED PROJECT (MIUR, ARS01_01081)
[1] S. Wilkens. (2015) F1000Prime Rep. 7, 14. [2] V. Vasiliou, K. Vasiliou, DW. Nebert (2009) Hum Genomics. 3(3):281-90. [3] F. Cuviello, F. Bisaccia, D. Spinelli, et al. (2019) Eur J Med Chem 164 1-7. [4] F. Martinelli, F. Cuviello, MC Pace et al. (2018) Cells. Front. Mol. Biosci., 5:75. [5] RS Jansen, S. Duijst, S. Mahakena, et al. . (2014) Arterioscler. Thromb. Vasc. Biol. 34, 1985–198
Vademecum. Il Protocollo eGLU 2.0. Come realizzare test di usabilità semplificati per i siti web delle PA
No full textL’obiettivo di coniugare competenza con trasparenza e usabilità si presenta come uno dei tratti distintivi che devono caratterizzare l’agire pubblico. La diffusione di uno strumento d’ausilio metodologico come il “Protocollo eGLU” qui nella versione 2.0, è coerente con questo imperativo. Aiutare a rafforzare le competenze professionali dei redattori web con uno strumento metodologicamente rigoroso messo a punto da specialisti per non specialisti, da una parte, e favorire, dall’altra, la gestione dei processi di verifica della qualità dei siti da parte delle amministrazioni con test di usabilità semplificati e quindi a basso costo, vuol dire infatti assicurare condizioni di reale efficacia e soddisfazione della comunicazione web
Inovação sistemática com responsabilidade social nos empreendimentos de base tecnológica: o modelo MCNS-TRIZ
No full textTese (doutorado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Engenharia e Gestão do Conhecimento, Florianópolis, 2010A Gestão do Conhecimento pressupõe o compartilhamento e a externalização de idéias propostas pelo capital intelectual das organizações, que, por sua vez, possibilitam a criação de novos conhecimentos que se traduzem em vantagem competitiva. Se a produtividade é uma preocupação do sistema empresa em seu ambiente interno, a competitividade é uma preocupação advinda do sistema externo ao microambiente empresarial. Dessa forma, a Inteligência Competitiva possibilita ir além da definição de estratégias, auxiliando na competitividade empresarial numa gestão pró-ativa. Para os empreendimentos que almejam liderança em tecnologia, a inovação sistemática permite rapidez e aceleração nos processos, no entanto, a inovação deve vir acompanhada de responsabilidade social, em reação a uma exigência da sociedade voltada para o Desenvolvimento Econômico Sustentável. A carência de um método que permita integrar o Capital Intelectual, a Gestão do Conhecimento, a Inteligência Competitiva aos modelos de negócios que permitam a inovação com responsabilidade social com vistas à sustentabilidade do negócio, aumentando o ciclo de vida e tornando o empreendimento perene no tempo na Sociedade do Conhecimento, levou o autor a propor o Método MCNS-TRIZ. Este é composto por 43 Princípios e permite conceber soluções criativas para os Modelos de Negócios, respeitando as expectativas dos diversos stakeholders, e de forma a atender a todos de uma maneira equilibrada, evitando soluções de compromisso. A aplicação se fez em um teste piloto e esse método foi inserido em um modelo conceitual e aplicado à criação de empreendimentos de base tecnológica, sendo útil na criação de modelos de negócios sustentáveis em sua fase de concepção. O modelo conceitual denominado de Modelo MCNS-TRIZ foi descrito em forma de modelagem de processos, sendo composto por três fases: idéia, método e modelo de negócios. Todas as fases incluem atividades e tarefas, num total de seis atividades e dezesseis tarefas. A verificação do modelo MCNS-TRIZ foi feita em dois estudos de caso, o caso número 1 com nível elevado de abstração, cuja seleção respeitou o Princípio da Idéia Inovadora, o caso número 2 com nível baixo de abstração, que respeitou o Princípio da Marca. Os resultados comprovaram a viabilidade técnica de aplicação do modelo e a necessidade de ajustes para aplicação a outros casos.A Gestão do Conhecimento pressupõe o compartilhamento e a externalização de idéias propostas pelo capital intelectual das organizações, que, por sua vez, possibilitam a criação de novos conhecimentos que se traduzem em vantagem competitiva. Se a produtividade é uma preocupação do sistema empresa em seu ambiente interno, a competitividade é uma preocupação advinda do sistema externo ao microambiente empresarial. Dessa forma, a Inteligência Competitiva possibilita ir além da definição de estratégias, auxiliando na competitividade empresarial numa gestão pró-ativa. Para os empreendimentos que almejam liderança em tecnologia, a inovação sistemática permite rapidez e aceleração nos processos, no entanto, a inovação deve vir acompanhada de responsabilidade social, em reação a uma exigência da sociedade voltada para o Desenvolvimento Econômico Sustentável. A carência de um método que permita integrar o Capital Intelectual, a Gestão do Conhecimento, a Inteligência Competitiva aos modelos de negócios que permitam a inovação com responsabilidade social com vistas à sustentabilidade do negócio, aumentando o ciclo de vida e tornando o empreendimento perene no tempo na Sociedade do Conhecimento, levou o autor a propor o Método MCNS-TRIZ. Este é composto por 43 Princípios e permite conceber soluções criativas para os Modelos de Negócios, respeitando as expectativas dos diversos stakeholders, e de forma a atender a todos de uma maneira equilibrada, evitando soluções de compromisso. A aplicação se fez em um teste piloto e esse método foi inserido em um modelo conceitual e aplicado à criação de empreendimentos de base tecnológica, sendo útil na criação de modelos de negócios sustentáveis em sua fase de concepção. O modelo conceitual denominado de Modelo MCNS-TRIZ foi descrito em forma de modelagem de processos, sendo composto por três fases: idéia, método e modelo de negócios. Todas as fases incluem atividades e tarefas, num total de seis atividades e dezesseis tarefas. A verificação do modelo MCNS-TRIZ foi feita em dois estudos de caso, o caso número 1 com nível elevado de abstração, cuja seleção respeitou o Princípio da Idéia Inovadora, o caso número 2 com nível baixo de abstração, que respeitou o Princípio da Marca. Os resultados comprovaram a viabilidade técnica de aplicação do modelo e a necessidade de ajustes para aplicação a outros casos.The Knowledge Management's presupposes the sharing and the declaration of ideas proposed by the Intellectual Capital of the organizations, that, for your time, making possible the creation of new knowledge that are translated in competitive advantage. If the productivity is a concern of the system company in your internal environment, the competitiveness is a concern from of the external system entrepreneurial. In that way, the Competitive Intelligence makes possible to go besides the definition of strategies, aiding in the managerial competitiveness to up administration for-active. For the enterprises that long for leadership in technology, the systematic innovation allows speed and acceleration in the processes; however, the innovation should come accompanied of social responsibility, in reaction to a demand of the society to the Maintainable Economical Development's. The lack of a method that allows to integrate the Intellectual Capital, the Knowledge Management's, the Competitive Intelligence to the models of businesses that allow the innovation with social responsibility with views to maintainable business's, increasing the life cycle and turning the perennial enterprise in the age in the Society of the Knowledge's are reasons in that the author of thesis propose the Method MCNS-TRIZ. That method is composed by 43 principles and its allows to conceive creative solutions for the Models of Businesses, respecting the expectations of the several stakeholders, and in way to assist all in a balanced way, avoiding commitment solutions. That application was in a pilot test and that method was inserted in a conceptual framework and applied to the creation of enterprises of technological base, being useful in the creation of models of maintainable businesses in your conception phase. The conceptual framework was called MCNS-TRIZ Model and was described in form of modeling of processes, being composed for three phases: idealizes method and model of businesses. All the phases included activities and tasks, in the total; they were defined six activities and sixteen tasks. The verification of the MCNS-TRIZ Model was made in two case studies, the case number 1 with high level of abstraction, whose selection respected the Principle of the Innovative Idea, the case number 2 with low level of abstraction that respected the Principle of the Brand. The results proved the technical viability of application of the model and the need of fittings for application the other cases
Autumnal Beach Litter Identification by Mean of Using Ground-Based IR Thermography
No full textThe progress of scientific research and technological innovation are contributing to an increase in the use of rapid systems for monitoring and identifying geo-environmental processes related to natural and/or anthropogenic activities. The aim of this study is identifying autumnal beach litter using ground-based IR thermography. Starting from quarterly autumn monitoring data of air temperature and sandy soil surface temperature, an empirical equation between the two environmental matrices (air and sandy soil) is obtained. This will allow the calculation of the sandy soil surface temperature knowing only the air temperature. Therefore, it will be possible to know in advance the thermal response of the sandy soil, thus creating a thermal blank of the beach. Using an IR thermal camera, it is possible for a quicker identification of thermal anomalies of the coastal area potentially connected to the presence of pollution due to the anthropogenic origin (particularly plastic material). The test area is located in the area of the Coastal Dunes Regional Natural Park of Ostuni–Fasano in Apulia (southern Italy)
Capsicum annuum L.cv senise and solanum aethiopicum L.cv Rotonda: phytochemical profile and cell-based assays
No full text2020 - 2021Natural products rich in health-promoting compounds could represent new potential therapy
strategy in many diseases.
In the present study we focus our attention on Capsicum annuum L. cv Senise and Solanum
aethiopicum L. cv Rotonda, two typical products of Basilicata region and source of health-promoting
compounds. Particularly, we investigated molecular pathways involved in anti-obesity activity
demonstrated in mice fed with high fat diet.
Firstly, antioxidant activity of samples was evaluated in HepG2 cell line. Cells were pre-treated with
different doses of the extracts and ROS generation was measured by flow cytometry. Expression of
several markers involved in antioxidant defence was evaluated by qRT-PCR in order to understand
molecular mechanism responsible of the activity.
Oxidative stress characterized several pathology conditions. About obesity, prolonged exposure of
excess of fat induced weight gain but also ROS and pro-inflammatory cytokines generation.
Hypolipidemic, antioxidant and anti-inflammatory activity of the extracts were evaluated in HepG2
and Caco-2 cell line used as model. Lipid accumulation was morphologically evaluated by Oil red O
staining and the expression of several markers involved in lipid metabolism, antioxidant defense and
inflammatory status was measured by qRT-PCR. Both extracts reduced fat accumulation in OA-
treated HepG2 cell line by reducing de novo lipogenesis and improved intestinal lipid absorption.
Moreover, both extracts enhance the activity of antioxidant enzymes severely compromised in
obesity.
Obesity is also associated with diabetes type 2 and cardiovascular risk. Senise pepper influenced the
expression of some gene involved in insulin signalling pathway improving glucose uptake as
demonstrated by 2-NBDG assay and 1H-NMR analysis. In addition, Senise pepper demonstrated
cardioprotective effect by improving endothelial dysfunction in obese mice (in collaboration with
Universidad de Granada) due to inhibition of NADPH oxidase and antioxidant and anti-inflammatory
activity demonstrated in OA-treated cells and splenocytes (in collaboration with Laboratory of
Immunotherapy, Institute of Microbiology of the Academy of Science Czech in Prague).
Finally, LC-MS was performed in order to identify the specialized compounds responsible of
biological activity of C.annnum L. cv Senise and S. aethiopicum L. cv Rotonda [edited by Author]XXXIII cicl
Biological synthesis of CdS/CdSe core/shell nanoparticles and its application in quantum dot sensitized solar cells
No full textIndexación: ScopusIn the present work, we report the use of bacterial cells for the production of CdS/CdSe Core/Shell quantum dots (QDs), a complex nanostructure specially designed to improve their performance as photosensitizer in photovoltaic devices. The method requires the incorporation of L-cysteine, CdCl2 and Na2SeO3 to Escherichia coli cultures and allows a tight control of QDs properties. The obtained CdS/CdSe QDs were photophysically and structurally characterized. When compared to CdS QDs, the classical shift in the UV-visible spectra of Core/Shell nanostructures was observed in CdS/CdSe QDs. The nanosize, structure, and composition of Core/Shell QDs were confirmed by TEM and EDS analysis. QDs presented a size of approximately 12 nm (CdS) and 17 nm (CdS/CdSe) as determined by dynamic light scattering (DLS), whereas the fourier transform infrared (FTIR) spectra allowed to distinguish the presence of different biomolecules bound to both types of nanoparticles. An increased photostability was observed in CdS/CdSe nanoparticles when compared to CdS QDs. Finally, biosynthesized CdS/CdSe Core/Shell QDs were used as photosensitizers for quantum dots sensitized solar cells (QDSSCs) and their photovoltaic parameters determined. As expected, the efficiency of solar cells sensitized with biological CdS/CdSe QDs increased almost 2.5 times when compared to cells sensitized with CdS QDs. This work is the first report of biological synthesis of CdS/CdSe Core/Shell QDs using bacterial cells and represents a significant contribution to the development of green and low-cost photovoltaic technologies. Copyright © 2019 Órdenes-Aenishanslins, Anziani-Ostuni, Quezada, Espinoza-González, Bravo and Pérez-Donoso. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.https://www.frontiersin.org/articles/10.3389/fmicb.2019.01587/ful
