14 research outputs found
What\u27s the Difference? Interpretation, Identity and R. v. R.D.S.
No full textLawyers hanker after authority. Whether it be in enforcing the law or justifying law\u27s institutional power, there is an almost desperate yearning to establish and maintain the legitimacy of law and, therefore, of themselves, in a social world in which the whole notion of authority is challenged and undermined. When it comes to matters of legal interpretation, jurists and judges still crave some method that will ground or trace back an interpretation to a foundational or ultimate source that can confer authority on one particular interpretation over another. However, recent jurisprudential debate has done fatal damage to the notion that meaning can ever be uncontroversially located in the text itself or its author\u27s intentions: the death of the author is part of the larger critical claim that there is no one to challenge or to speak in the name of because to refuse to arrest meaning is finally to refuse God and his hypostases, reason, science, the law. \u27 Nonetheless, in casting interpretation as an open practice of creative re-construction, a non-foundational approach is not to be taken as claiming that the identity of legal operatives in law\u27s language game is somehow unimportant or irrelevant. To ignore entirely the fact that someone is speaking is as much a mistake as depending exclusively on who is speaking to determine meaning
Zeven principes voor werken aan gezonde gebiedsontwikkeling
No full textGebiedsontwikkeling die uitgaat van een gezond leven raakt aan de kern van ons bestaan. Als begrip is gezonde gebiedsontwikkeling in opkomst, maar er zijn nog weinig handvatten voorhanden voor gebiedsontwikkelaars, in denken en doen. Daarom formuleren adviseur en onderzoeker Wouter Jan Verheul en collega’s zeven principes voor het werken aan gezonde gebiedsontwikkeling.Urban Development Managemen
A protein methylation pathway in Chlamydomonas flagella is active during flagellar resorption
No full textAuthor Posting. © American Society for Cell Biology, 2008. This article is posted here by permission of American Society for Cell Biology for personal use, not for redistribution. The definitive version was published in Molecular Biology of the Cell 19 (2008): 4319-4327, doi:10.1091/mbc.E08-05-0470.During intraflagellar transport (IFT), the regulation of motor proteins, the loading and unloading of cargo and the turnover of flagellar proteins all occur at the flagellar tip. To begin an analysis of the protein composition of the flagellar tip, we used difference gel electrophoresis to compare long versus short (i.e., regenerating) flagella. The concentration of tip proteins should be higher relative to that of tubulin (which is constant per unit length of the flagellum) in short compared with long flagella. One protein we have identified is the cobalamin-independent form of methionine synthase (MetE). Antibodies to MetE label flagella in a punctate pattern reminiscent of IFT particle staining, and immunoblot analysis reveals that the amount of MetE in flagella is low in full-length flagella, increased in regenerating flagella, and highest in resorbing flagella. Four methylated proteins have been identified in resorbing flagella, using antibodies specific for asymmetrically dimethylated arginine residues. These proteins are found almost exclusively in the axonemal fraction, and the methylated forms of these proteins are essentially absent in full-length and regenerating flagella. Because most cells resorb cilia/flagella before cell division, these data indicate a link between flagellar protein methylation and progression through the cell cycle.This work was supported by National Institutes
of Health Grant DK071720 (R.D.S.) and National Science Foundation Grant
MCB 0418877 (R.D.S.)
Entry words for Indonesian names
No full textCatalog is a representative of document and Entry Name is one of important part of catalog. For cataloguing purpose, Indonesian librarians used the Peraturan penentuan nama-nama Indonesia (Rules for Indonesian names) published by National Scientific Documentation Centre and recognized as the Indonesian National Standard, first published in 1975 and accepted as one of the Standar Nasional Indonesia or Indonesian National Standard. However it never been revised since its acceptance as a standard although the regulation stated that any standard must be revised every five years. The other cataloguing rule is "Peraturan katalogisasi nama-nama Indonesia" [Cataloging rules for Indonesian names] published by Pusat Pembinaan Perpustakaan (Center for Library Development) in 1976, later on continued by the Indonesian National Library as Peraturan katalogisasi Indonesia (Indonesian cataloguing rules), the last edition is the 4th edition published in 1996 and also never been revised since then. Owing to the different users, usage and dissemination, the "Peraturan penentuan nama-nama
Indonesia" is widely used among academic and special libraries as well as becoming the cataloguing standards at various library schools and cited by lecturers for entering
Indonesian names in their bibliographies. The author is speaking on behalf of The National Library of Indonesi
Protein arginine methyltransferases interact with intraflagellar transport particles and change location during flagellar growth and resorption
No full text© The Author(s), 2017. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Molecular Biology of the Cell 28 (2017): 1208-1222, doi:10.1091/mbc.E16-11-0774.Changes in protein by posttranslational modifications comprise an important mechanism for the control of many cellular processes. Several flagellar proteins are methylated on arginine residues during flagellar resorption; however, the function is not understood. To learn more about the role of protein methylation during flagellar dynamics, we focused on protein arginine methyltransferases (PRMTs) 1, 3, 5, and 10. These PRMTs localize to the tip of flagella and in a punctate pattern along the length, very similar, but not identical, to that of intraflagellar transport (IFT) components. In addition, we found that PRMT 1 and 3 are also highly enriched at the base of the flagella, and the basal localization of these PRMTs changes during flagellar regeneration and resorption. Proteins with methyl arginine residues are also enriched at the tip and base of flagella, and their localization also changes during flagellar assembly and disassembly. PRMTs are lost from the flagella of fla10-1 cells, which carry a temperature-sensitive mutation in the anterograde motor for IFT. The data define the distribution of specific PRMTs and their target proteins in flagella and demonstrate that PRMTs are cargo for translocation within flagella by the process of IFT.This work was supported by National Science Foundation Award MCB 0950402 (R.D.S.), the Ira Allen Eastman (Class of 1829) Professorship at Dartmouth (R.D.S.), which was established in 1910 through a gift to the College by his widow, Jane Eastman, and by a Postdoctoral Fellowship for Research Abroad from the Japan Society for the Promotion of Science (K.M.)
Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis
No full textWe are grateful to Torcato Martins for insightful scientific discussions. We also thank Cláudia Florindo for assistance in fluorescence microscopy, Margarida Neto for maintenance of Drosophila stock collection and technical support, and Alexandra Tavares for technical support. We acknowledge the TRiP collection at Harvard Medical School and BDSC for providing several of the Drosophila stocks used in this study. We acknowledge and thank Claudio E. Sunkel for antibodies. B.M. and R.D.S. are both supported by Portuguese national funding through Fundação para a Ciência e a Tecnologia (within the scope of the ProRegeM PhD program; PD/ 00117/2012, CRM:0027030) and DL 57/2016/CP1361/CT0019. The Light Microscopy Unit of ABC-RI was partially supported by Portuguese national funding (FCT: PPBI-POCI-01-0145-FEDER-022122). This work was developed with the support of the research infrastructure Congento (project LISBOA-01-0145-FEDER-022170). The Transgenic RNAi Project (TRiP) collection at Harvard Medical School was supported by NIH/NIGMS R01-GM084947. The funding bodies had no role in the design of this study; collection, analysis, and interpretation of data; and manuscript writing.
Publisher Copyright:
Copyright © 2022 the Author(s).The synaptonemal complex (SC) is a proteinaceous scaffold that is assembled between paired homologous chromosomes during the onset of meiosis. Timely expression of SC coding genes is essential for SC assembly and successful meiosis. However, SC components have an intrinsic tendency to self-organize into abnormal repetitive structures, which are not assembled between the paired homologs and whose formation is potentially deleterious for meiosis and gametogenesis. This creates an interesting conundrum, where SC genes need to be robustly expressed during meiosis, but their expression must be carefully regulated to prevent the formation of anomalous SC structures. In this manuscript, we show that the Polycomb group protein Sfmbt, the Drosophila ortholog of human MBTD1 and L3MBTL2, is required to avoid excessive expression of SC genes during prophase I. Although SC assembly is normal after Sfmbt depletion, SC disassembly is abnormal with the formation of multiple synaptonemal complexes (polycomplexes) within the oocyte. Overexpression of the SC gene corona and depletion of other Polycomb group proteins are similarly associated with polycomplex formation during SC disassembly. These polycomplexes are highly dynamic and have a well-defined periodic structure. Further confirming the importance of Sfmbt, germ line depletion of this protein is associated with significant metaphase I defects and a reduction in female fertility. Since transcription of SC genes mostly occurs during early prophase I, our results suggest a role of Sfmbt and other Polycomb group proteins in downregulating the expression of these and other early prophase I genes during later stages of meiosis.publishersversionpublishe
Experimental Balancing Technique By Trial Masses
No full textThis paper approaches the static and dynamic experimental balancing, applied to rotating machinery, considering the trial Weight Balancing. This method uses the Fast Fourier Transform (FFT), intending to determine the vibration signal (amplitude and phase) and to balance the system. This behavior occurs, because of the eccentricity of the center of gravity of th disk. One of the objectives is to allow a precision balancing of rotating systems, in order to apply fitting process to balanced rotating systems, where it is possible to include a known unbalance, which can be use to check some fitting methods in laboratory. The data acquisition is made by proximity sensors located at the disk or at the journal bearings. These data are processed by the software LabView, where the vibration signal is evaluated and the masses necessary to balance and their angular positions on the disk are determined. Copyright © 2005 Society of Automotive Engineers, Inc.Collacott, R.A., (1979) Vibration Monitoring and Diagnosis, , John Willey & sons, New YorkLabVIEW (1998) Data Aquisition Basics Manual, , National InstrumentsBraun, S., (1986) Mechanical Signature Analysis, , Academic Press Inc. LondonNewland, D.E., (1975) An Introduction to Randon Vibration and Spectral Analysis, , Longman Group Limited, LondonDally, J.W., Rilley, W.F., McConnel, K.G., (1993) Instrumentation for Enginerring Measurements, , John Willey & Sons, NYMcConnel, K.G., (1995) Vibration Testing: Theory and Pratic, , John Willey & Sons, NYGenta, G., (1993) Vibration Os Structures and Machines, pp. 313-314. , Pratical aspects. Springer-Verlagwww.sdid.uc.edu, Structures/Motion Lab 20-263-571, Sections 001, 002, 003Dovel, G., S&V Observer, , Hawlett-Packard Company, Everett, WashingtonAlmeida, M.T.D., Góz, R.D.S., (1999) FUPAI, , Balanceamento de RotoresVictor Wowk, P.E., A Management Guide to Balancing, , the president of Machine Dynamics, Inc. 3540B Pan American Fwy, NE, Albuquerque, NM 87107, and the author of Machinery Vibration: Balancing ISBN 0-07-071938-1 published by McGraw-HillKroon, R.P., Thearle, E.L., Field Balancing Large Rotating Machinery (1944) Journal of Applied Mechanics, 11 (1). , Balancing of Rotating Apparatus II, March. Dynamic Balancing of Rotating Machinery in the Field, translation of the ASME, October 1934Online Reference - Learn with activities LabVeiw 5.0, National Instrumentes®Meriam, J.L., Kraige, L.G., (1999), Dinâmica, 4a edição, translation to portuguese, LTC, Rio de Janeir
Cryptosporidium Spp. And Giardia Spp. In Feces And Water And The Associated Exposure Factors On Dairy Farms
No full textThe aims of this study were to verify the prevalence of Cryptosporidium spp. and Giardia spp. in animal feces and drinking water on dairy farms and to identify a possible relation between the exposure factors and the presence of these parasites. Fecal samples from cattle and humans and water samples were collected on dairy farms in Paraná, Brazil. Analysis of (oo)cysts in the feces was performed by the modified Ziehl-Neelsen staining and centrifugal flotation in zinc sulfate. Test-positive samples were subjected to nested PCR amplification of the 18SSU ribosomal RNA gene for identification of Cryptosporidium and Giardia and of the gp60 gene for subtyping of Cryptosporidium. Microbiological analysis of water was carried out by the multiple-tube method and by means of a chromogenic substrate, and parasitological analysis was performed on 31 samples by direct immunofluorescence and nested PCR of the genes mentioned above. Identification of the species of Cryptosporidium was performed by sequencing and PCR with analysis of restriction fragment length polymorphisms. The prevalence of Giardia and Cryptosporidium was higher in calves than in adults. Among the samples of cattle feces, Cryptosporidium parvum was identified in 41 (64%), C. ryanae in eight (12.5%), C. bovis in four (6.3%), C. andersoni in five (7.8%), and a mixed infection in 20 samples (31.3%). These parasites were not identified in the samples of human feces. Thermotolerant coliform bacteria were identified in 25 samples of water (45.5%). Giardia duodenalis and C. parvum were identified in three water samples. The gp60 gene analysis of C. parvum isolates revealed the presence of two strains (IIaA20G1R1 and IIaA17G2R2) in the fecal samples and one (IIaA17G2R1) in the water samples. The presence of coliforms was associated with the water source, structure and degradation of springs, rain, and turbidity. The prevalence of protozoa was higher in calves up to six months of age. C. parvum and G. duodenalis were identified in the water of dairy farms, as were thermotolerant coliforms; these findings point to the need for guidance on handling of animals, preservation of water sources, and water treatment. © 2017 Toledo et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.12
KIC 3858884: A hybrid ? Scuti pulsator in a highly eccentric eclipsing binary
No full textAnalysis of eclipsing binaries containing non-radial pulsators allows i) combining two different and independent sources of information on the internal structure and evolutionary status of the components and ii) studying the effects of tidal forces on pulsations. KIC 3858884 is a bright Kepler target whose light curve shows deep eclipses, complex pulsation patterns with pulsation frequencies typical of ? Sct, and a highly eccentric orbit. We present the result of the analysis of Kepler photometry and of high resolution phase-resolved spectroscopy. Spectroscopy yielded both the radial velocity curves and, after spectral disentangling, the primary-component effective temperature and metallicity, and line-of-sight projected rotational velocities. The Kepler light curve was analyzed with an iterative procedure that was devised to disentangle eclipses from pulsations and takes the visibility of the pulsating star into account during eclipses. The search for the best set of binary parameters was performed by combining the synthetic light curve models with a genetic minimization algorithm, which yielded a robust and accurate determination of the system parameters. The binary components have very similar masses (1.88 and 1.86 M⊙) and effective temperatures (6800 and 6600 K), but different radii (3.45 and 3.05 R ⊙). The comparison with the theoretical models showed a somewhat different evolutionary status of the components and the need to introduce overshooting in the models. The pulsation analysis indicates the hybrid nature of the pulsating (secondary) component, where the corresponding high order g-modes might be excited by an intrinsic mechanism or by tidal forces. © ESO, 2014.Belgian Federal Science Policy Office: PRODEX – CoRoT The Research Council Agenzia Spaziale Italiana Space Telescope Science Institute Istituto Nazionale di Astrofisica Pennsylvania State University National Council for Scientific Research: TÜBİTAK 111T270 National Aeronautics and Space Administration: NAS5-26555 Istituto Nazionale di Astrofisica FNRS Fonds Wetenschappelijk Onderzoek Agenzia Spaziale Italiana Applied Scientific Research Fund National Science Foundation: AST 1006676, AST 1126413 NNX13AC07G Pennsylvania Space Grant Consortium? Scuti pulsator in a highly eccentric eclipsing binary ? ?? ??? ? Based on photometry collected by the Kepler space mission. ?? Full Table B.1 is only available at the CDS via anonymous ftp to cdsarc.u-strasbg.fr ( 130.79.128.5 ) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/563/A59 ??? Appendices are available in electronic form at http://www.aanda.org Maceroni C. 1 Lehmann H. 2 da Silva R. 1 Montalbán J. 3 Lee C.-U. 4 Ak H. 5 6 Deshpande R. 6 7 Yakut K. 8 Debosscher J. 9 Guo Z. 10 Kim S.-L. 4 Lee J. W. 4 Southworth J. 11 1 INAF – Osservatorio astronomico di Roma , via Frascati 33 , 00040 Monteporzio C. , Italy 2 Thüringer Landessternwarte Tautenburg , Sternwarte 5 , 07778 Tautenburg , Germany 3 Institut d’Astrophysique et Géophysique Université de Liège , Allée du 6 Aôut , 4000 Liège , Belgium 4 Korea Astronomy and Space Science Institute , 305-348 Daejeon , Korea 5 Erciyes University, Science Faculty , Astronomy and Space Sci. Dept. , 38039 Kayseri , Turkey 6 Department of Astronomy and Astrophysics, The Pennsylvania State University , University Park , PA 16802 , USA 7 Center for Exoplanets and Habitable Worlds, The Pennsylvania State University , University Park , PA 16802 , USA 8 Department of Astronomy & Space Sciences, University of Ege , 35100 İzmir , Turkey 9 Instituut for Sterrenkunde, K.U. Leuven , Celestijnenlaan 200 D , 3001 Leuven , Belgium 10 Center for High Angular Resolution Astronomy and Department of Physics and Astronomy, Georgia State University , PO Box 5060 , Atlanta GA 30302-5060 , USA 11 Astrophysics Group, Keele University , Staffordshire ST5 5BG , UK e-mail: [email protected] 07 03 2014 07 03 2014 3 2014 563 aa/2014/03 A59 18 10 2013 12 1 2014 © ESO, 2014 2014 ESO Analysis of eclipsing binaries containing non-radial pulsators allows i) combining two different and independent sources of information on the internal structure and evolutionary status of the components and ii) studying the effects of tidal forces on pulsations. KIC 3858884 is a bright Kepler target whose light curve shows deep eclipses, complex pulsation patterns with pulsation frequencies typical of ? Sct, and a highly eccentric orbit. We present the result of the analysis of Kepler photometry and of high resolution phase-resolved spectroscopy. Spectroscopy yielded both the radial velocity curves and, after spectral disentangling, the primary-component effective temperature and metallicity, and line-of-sight projected rotational velocities. The Kepler light curve was analyzed with an iterative procedure that was devised to disentangle eclipses from pulsations and takes the visibility of the pulsating star into account during eclipses. The search for the best set of binary parameters was performed by combining the synthetic light curve models with a genetic minimization algorithm, which yielded a robust and accurate determination of the system parameters. The binary components have very similar masses (1.88 and 1.86 M ? ) and effective temperatures (6800 and 6600 K), but different radii (3.45 and 3.05 R ? ). The comparison with the theoretical models showed a somewhat different evolutionary status of the components and the need to introduce overshooting in the models. The pulsation analysis indicates the hybrid nature of the pulsating (secondary) component, where the corresponding high order g-modes might be excited by an intrinsic mechanism or by tidal forces. binaries: eclipsing binaries: spectroscopic stars: fundamental parameters stars: interiors stars: oscillations stars: individual: KIC 3858884 idline A&A 563, A59 (2014) cover_date March 2014 first_month 3 last_month 3 first_year 2014 last_year 2014 We thank Conny Aerts for enlightening discussions and critically reading the manuscript, and Daniel Reese, Avi Shporer, Orlagh Creevey, Kevin Stassun for useful suggestions. We also express our gratitude to Andrej Prša for making PHOEBE publicly available, and for his constant support; to Paul Charbonneau, author of PIKAIA; and to Marc Antoine Dupret for making his computer code MAD available to us. We also thank the unknown referee for constructive criticisms. This work is partly based on data from the Kepler mission, which is funded by the NASA Science Mission directorate. The photometric data were obtained from the Mikulski Archive for Space Telescopes (MAST). STScI is operated by the Association of Universities for Research in Astronomy, Inc., under NASA contract NAS5-26555. Support for MAST for non-HST data is provided by the NASA Office of Space Science via grant NNX13AC07G and by other grants and contracts. This research is also partly based on observations obtained with the HERMES spectrograph, which is supported by the Fund for Scientific Research of Flanders (FWO), Belgium, the Research Council of K.U. Leuven, Belgium, the Fonds National Recherches Scientific (FNRS), Belgium, the Royal Observatory of Belgium, the Observatoire de Genève, Switzerland, and the Thüringer Landessternwarte Tautenburg, Germany; HERMES is attached to the Mercator Telescope, which is operated on the island of La Palma by the Flemish Community and is located at the Spanish Observatorio del Roque de los Muchachos of the Instituto de Astrofisica de Canarias. Additional data were obtained at the Hobby-Eberly Telescope (HET), a joint project of the University of Texas at Austin, Pennsylvania State University, Stanford University, Ludwig-Maximilians- Universitat Munchen, and Georg-August-Universitat Gottingen. We also made use of the VO-KOREL web service, developed at the Astronomical Institute of the Academy of Sciences of the Czech Republic in the framework of the Czech Virtual Observatory (CZVO) by P. Skoda and J. Fuchs using the Fourier disentangling code KOREL by P. Hadrava. The authors, finally, acknowledge generous financial support: from the Istituto Nazionale di Astrofisica (INAF) under PRIN-2010 Asteroseismology: looking inside the stars with space- and ground-based observations and from the Agenzia Spaziale Italiana (ASI) in the frame of the ESS program (C.M. and R.d.S); from BELSPO under contract PRODEX – CoRoT (J.M.), from NSF under grant AST 1006676 and AST 1126413 (R.D.), from the Turkish Scientific and Research Council (TÜBİTAK 111T270) (KY), and from the Center for Exoplanets and Habitable Worlds (R.D.), which is supported by the Pennsylvania State University, the Eberly College of Science, and the Pennsylvania Space Grant Consortium. -
The biogeography of the Amazonian tree flora
No full textWe describe the geographical variation in tree species composition across Amazonian forests and show how environmental conditions are associated with species turnover. Our analyses are based on 2023 forest inventory plots (1 ha) that provide abundance data for a total of 5188 tree species. Within-plot species composition reflected both local environmental conditions (especially soil nutrients and hydrology) and geographical regions. A broader-scale view of species turnover was obtained by interpolating the relative tree species abundances over Amazonia into 47,441 0.1-degree grid cells. Two main dimensions of spatial change in tree species composition were identified. The first was a gradient between western Amazonia at the Andean forelands (with young geology and relatively nutrient-rich soils) and central–eastern Amazonia associated with the Guiana and Brazilian Shields (with more ancient geology and poor soils). The second gradient was between the wet forests of the northwest and the drier forests in southern Amazonia. Isolines linking cells of similar composition crossed major Amazonian rivers, suggesting that tree species distributions are not limited by rivers. Even though some areas of relatively sharp species turnover were identified, mostly the tree species composition changed gradually over large extents, which does not support delimiting clear discrete biogeographic regions within Amazonia
