1,721,468 research outputs found
Studies of the interaction of novel forms of the p185(c-neu) receptor ectodomain
No full textWe are exploring the roles of the extracellular domain (ECD) of the rat p185c-neu, one member of the erbB family transmembrane receptor tyrosine kinases, which features a signal diversifying mechanism. This mechanism involves the formation of various homo- and hetero-oligomers. One set of the rat c-neu extracellular subdomain individually-deleted constructs and their corresponding NR6 transfectants with or without the coexpression of other erbB family members were studied for inter-receptor interactions. A c-neu-IgG(human) immunoadhesin was also developed to search for the rat p185c-neu ECD specific interacting proteins and to generate a rabbit polyclonal antibody, Anti-NEX. We found that the rat c-neu extracellular subdomain deletions significantly limited cell surface targeting of these mutant receptors, especially in single transfectants. The deletions at the c-neu different extracellular subdomains resulted in distinct changes of the mutant c-neu receptors\u27 phosphotyrosine (PY) content. Extracellular treatment with Anti-NEX significantly down-modulated p185c-neu and increased the PY content of the remaining p185 c-neu. Rat E18 frozen sections stained by high concentrations of the c-neu-IgG(human) immunoadhesin showed a pattern parallel to p185c-neu tissue distribution pattern. Two lines of the c-neu-IgG(human) transgenic driven by the human cytomegalovirus promoter/enhancer were established. RT-PCR assay detected the transcripts of the c-neu-IgG(human) transgene and the mouse c-neu in all examined organs. The c-neu-IgG(human) protein was also detectable in the plasma. But the c-neu-IgG(human) transgenic mice lacked any obvious phenotype. These studies reveal novel regulatory roles of the p185 c-neu ECD. The results of this dissertation may provide new thoughts for cancer therapy and protein engineering
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Effect of c-neu/ErbB2 Expression Levels on Estrogen Receptor alpha-Dependent Proliferation in Mammary Epithelial Cells: Implication for Breast Cancer Biology
No full textMammary development and tumorigenesis are profoundly influenced by signaling pathways under the control of c-erbB2/c-neu and estrogen receptor α (ERα). Signaling through ERα is essential for ductal growth during puberty. In mice overexpressing wild-type c-neu in mammary epithelial cells, Tg (c-neu), ductal growth is impaired. An impeded signaling through ERα is also observed in a subset of human mammary tumors that overexpress erbB2. However, ductal growth is also impaired in the absence of c-neu in mouse mammary epithelial cells. To resolve this apparent paradox, we examined the relationship between c-neu expression and estrogen/ERα–dependent cell proliferation in pubertal Tg (c-neu). We report that proliferation in both terminal end buds and ducts is associated with ERα-positive cells, including those that coexpress c-neu, and is abolished in the absence of circulating estradiol. Tg (c-neu) contains hyperplastic mammary ducts with high proliferative index and coexpression of both ERα and c-neu in the dividing cells. These findings suggest that c-neu promotes ERα-dependent proliferation, and that this is responsible for the presence of hyperplastic ducts. Some of the hyperplastic ducts have acinar structures, indicative of morphologic differentiation. These ducts have low proliferative index and accompanied by a vast decrease in proliferation of ERα-positive cells, including those that express c-neu. As such, c-neu has dual but opposing effects on ERα-dependent proliferation in mammary epithelial cells. Therefore, depending on the physiologic setting, ductal morphogenesis will be compromised both in the absence and overexpression of c-neu, thus explaining the paradox. (Cancer Res 2006; 66(21): 10391-8
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
- …
