2,777 research outputs found
The Fairy Goblet of Eden Hall to Hunting Mammoths in the Rain - experiencing the paraxial through performance magic and mystery entertainment.
No full textThe performance piece Fairy Goblet of Eden Hall appeared in the Annemann's Jinx in 1941, it describes a ritual where each 'guest'' is invited to step forward to experience and share a vision of a past memory revealed to them by Titania's Fairy Goblet in a 'weird and uncanny manner''. Hunting Mammoths in the Rain was published in 2007 and demonstrates how an unearthly symbol discovered on an ancient rock can lead to a primal and olfactory experience for an audience. Both pieces represent key experiments in underground experiential performance magic.
In such pieces the magician (or, more accurately, the mystery entertainer) acts as a facilitator guiding the guests/audience through a form of ostensive magical behaviour. This paper will explore a number of these performance magic experiments drawing on the notion of the ‘paraxial’ (Mangan, 2007), and will examine how the mystery entertainer places themselves in a performative grey area situated between illusion and disillusion. And how the notion of the paraxial has continued to manifest in experiential performance magic in areas such as séance, mystery entertainment, mesmerism and story-telling magic
Peningkatan Jumlah Sel Goblet Aktif pada Duodenum Broiler yang Dipapar Heat Stress
No full textThe purpose of this study was to know the intestinal barrier response by observing number ofactivated goblet cells as mucin producer in the duodenum of broiler exposed by heat stress. Twenty DOC (Day Old Chick) broiler Cobb strain divided randomly into two groups. P0 (without heat stress treatment) was given the normal temperature. P1 (with heat stress treatment) was given temperature 35-35.5°C during four weeks (8 hours/day). Collected data for total count of activated goblet cells were analyzed with independent t-test. The results indicated that heat stress was very significantly increasing(p<0.01) number of activated goblet cells in duodenum of broiler. The conclusion for this research was high temperature could cause the increase of the number of activated goblet cells.
Heat stress, Goblet Cell, Broile
Aqueous Tear Deficiency Increases Conjunctival Interferon-c (IFN-c) Expression and Goblet Cell Loss
No full textPURPOSE. To investigate the hypothesis that increased interferon-c (IFN-c) expression is associated with conjunctival goblet cell loss in subjects with tear dysfunction. METHODS. Goblet cell density (GCD) was measured in impression cytology from the temporal bulbar conjunctiva, and gene expression was measured in cytology samples from the nasal bulbar conjunctiva obtained from 68 subjects, including normal control, meibomian gland disease (MGD), non-Sjögren syndrome (non-SSATD)-, and Sjögren syndrome (SSATD)-associated aqueous tear deficiency. Gene expression was evaluated by real-time PCR. Tear meniscus height (TMH) was measured by optical coherence tomography. Fluorescein and lissamine green dye staining evaluated corneal and conjunctival disease, respectively. Between-group mean differences and correlation coefficients were calculated. RESULTS. Compared to control, IFN-c expression was significantly higher in both ATD groups, and its receptor was higher in SSATD. Expression of IL-13 and its receptor was similar in all groups. Goblet cell density was lower in the SSATD group; expression of MUC5AC mucin was lower and cornified envelope precursor small proline-rich region (SPRR)-2G higher in both ATD groups. Interferon-c transcript number was inversely correlated with GCD (r ¼ À0.37, P < 0.04) and TMH (r ¼ À0.37, P ¼ 0.02), and directly correlated with lissamine green staining (r ¼ 0.51, P < 0.001) and SPRR-2G expression (r ¼ 0.32, P < 0.05). CONCLUSIONS. Interferon-c expression in the conjunctiva was higher in aqueous deficiency and correlated with goblet cell loss and severity of conjunctival disease. These results support findings of animal and culture studies showing that IFN-c reduces conjunctival goblet cell number and mucin production
Efek Vitamin C dan E Terhadap Sel Goblet Saluran Nafas pada Tikus Akibat Pajanan Asap Rokok
No full textPenelitian terdahulu menunjukkan bahwa pada perokok dengan bronkitis kronis dan penyakit paru obstruktif
kronis (PPOK) terdapat hipersekresi mukus dan hiperplasia sel goblet pada saluran nafas. Beberapa penelitian lain
juga menyatakan bahwa faktor utama yang menyebabkan hiperplasia sel goblet adalah pajanan asap rokok pada paru
secara kronis. Penelitian ini bertujuan untuk menilai efek antioksidan vitamin C dan E terhadap sel goblet saluran nafas
akibat asap rokok. Rancangan eksperimental posttest only control group digunakan pada 40 ekor hewan coba tikus
dewasa jantan galur Sprague-Dawley dengan berat badan 200-250 gram. Keempatpuluh hewan coba secara acak
dibagi menjadi empat kelompok yaitu kelompok kontrol, kelompok yang hanya diberikan asap rokok, kelompok yang
diberikan asap rokok + vitamin C, dan kelompok yang diberikan asap rokok + vitamin E. Kelompok perlakuan diberi
paparan asap rokok sebanyak 8 batang per hari dalam waktu 30 menit selama 20 hari. Hasil penelitian menunjukkan
bahwa terdapat perbedaan bermakna jumlah sel goblet antara kelompok kontrol dan kelompok perlakuan yang hanya
diberikan asap rokok (p= 0,000), ini berarti bahwa terjadi hiperplasia sel goblet akibat asap rokok. Dan tidak terdapat
perbedaan yang bermakna jumlah sel goblet antara kelompok kontrol dan kelompok perlakuan yang diberikan asap
rokok + vitamin C dan E (p = 0,816), ini menunjukan adanya efek antioksidan yang menurunkan terjadinya hyperplasia
sel goblet. Studi ini menunjukkan pemberian vitamin C dan E pada hewan coba yang diberi pajanan asap rokok
mempunyai manfaat untuk mencegah terjadinya hiperplasi sel goblet
Goblet cells need some stress
No full textThe intestinal tract is protected by epithelium-covering mucus, which is constantly renewed by goblet cells, a specialized type of epithelial cell. Mucus is largely composed of MUC2 mucin, an enormous molecule that poses a high demand on the endoplasmic reticulum (ER) for proper folding and protein assembly, creating a challenge for the secretory machinery in goblet cells. In this issue of the JCI, Grey et al. reveal that the ER resident protein and folding sensor ERN2 (also known as IRE1β) was instrumental for goblet cells to produce sufficient amounts of mucus to form a protective mucus layer. In the absence of ERN2, mucus production was reduced, impairing the mucus barrier, which allowed bacteria to penetrate and cause an epithelial cell stress response. This study emphasizes the importance of a controlled unfolded protein response (UPR) for goblet cell secretion
ENETS Consensus Guidelines for Neuroendocrine Neoplasms of the Appendix (Excluding Goblet Cell Carcinomas)
No full textConsensus Guidelines for Neuroendocrine Neoplasms of the Appendix (Excluding Goblet Cell Carcinomas)
Table_1_Mucosal affairs: glycosylation and expression changes of gill goblet cells and mucins in a fish–polyopisthocotylidan interaction.XLSX [Dataset]
No full text[Introduction]: Secreted mucins are highly O-glycosylated glycoproteins produced by goblet cells in mucosal epithelia. They constitute the protective viscous gel layer overlying the epithelia and are involved in pathogen recognition, adhesion and expulsion. The gill polyopisthocotylidan ectoparasite Sparicotyle chrysophrii, feeds on gilthead seabream (Sparus aurata) blood eliciting severe anemia.[Methods]: Control unexposed and recipient (R) gill samples of gilthead seabream experimentally infected with S. chrysophrii were obtained at six consecutive times (0, 11, 20, 32, 41, and 61 days post-exposure (dpe)). In histological samples, goblet cell numbers and their intensity of lectin labelling was registered. Expression of nine mucin genes (muc2, muc2a, muc2b, muc5a/c, muc4, muc13, muc18, muc19, imuc) and three regulatory factors involved in goblet cell differentiation (hes1, elf3, agr2) was studied by qPCR. In addition, differential expression of glycosyltransferases and glycosidases was analyzed in silico from previously obtained RNAseq datasets of S. chrysophrii-infected gilthead seabream gills with two different infection intensities.[Results and Discussion]: Increased goblet cell differentiation (up-regulated elf3 and agr2) leading to neutral goblet cell hyperplasia on gill lamellae of R fish gills was found from 32 dpe on, when adult parasite stages were first detected. At this time point, acute increased expression of both secreted (muc2a, muc2b, muc5a/c) and membrane-bound mucins (imuc, muc4, muc18) occurred in R gills. Mucins did not acidify during the course of infection, but their glycosylation pattern varied towards more complex glycoconjugates with sialylated, fucosylated and branched structures, according to lectin labelling and the shift of glycosyltransferase expression patterns. Gilthead seabream gill mucosal response against S. chrysophrii involved neutral mucus hypersecretion, which could contribute to worm expulsion and facilitate gas exchange to counterbalance parasite-induced hypoxia. Stress induced by the sparicotylosis condition seems to lead to changes in glycosylation characteristic of more structurally complex mucins.Peer reviewe
Table_2_Mucosal affairs: glycosylation and expression changes of gill goblet cells and mucins in a fish–polyopisthocotylidan interaction.XLSX [Dataset]
No full text[Introduction]: Secreted mucins are highly O-glycosylated glycoproteins produced by goblet cells in mucosal epithelia. They constitute the protective viscous gel layer overlying the epithelia and are involved in pathogen recognition, adhesion and expulsion. The gill polyopisthocotylidan ectoparasite Sparicotyle chrysophrii, feeds on gilthead seabream (Sparus aurata) blood eliciting severe anemia.[Methods]: Control unexposed and recipient (R) gill samples of gilthead seabream experimentally infected with S. chrysophrii were obtained at six consecutive times (0, 11, 20, 32, 41, and 61 days post-exposure (dpe)). In histological samples, goblet cell numbers and their intensity of lectin labelling was registered. Expression of nine mucin genes (muc2, muc2a, muc2b, muc5a/c, muc4, muc13, muc18, muc19, imuc) and three regulatory factors involved in goblet cell differentiation (hes1, elf3, agr2) was studied by qPCR. In addition, differential expression of glycosyltransferases and glycosidases was analyzed in silico from previously obtained RNAseq datasets of S. chrysophrii-infected gilthead seabream gills with two different infection intensities.[Results and Discussion]: Increased goblet cell differentiation (up-regulated elf3 and agr2) leading to neutral goblet cell hyperplasia on gill lamellae of R fish gills was found from 32 dpe on, when adult parasite stages were first detected. At this time point, acute increased expression of both secreted (muc2a, muc2b, muc5a/c) and membrane-bound mucins (imuc, muc4, muc18) occurred in R gills. Mucins did not acidify during the course of infection, but their glycosylation pattern varied towards more complex glycoconjugates with sialylated, fucosylated and branched structures, according to lectin labelling and the shift of glycosyltransferase expression patterns. Gilthead seabream gill mucosal response against S. chrysophrii involved neutral mucus hypersecretion, which could contribute to worm expulsion and facilitate gas exchange to counterbalance parasite-induced hypoxia. Stress induced by the sparicotylosis condition seems to lead to changes in glycosylation characteristic of more structurally complex mucins.Peer reviewe
Transmission electron micrographs of mouse, rat and human Peyer's patches show secreting goblet cells.
No full text<p>(A) Secreting goblet cell in mouse FAE. (B) M cell in mouse FAE. (C) Secreting goblet cell in a rat FAE. (D) Two M cells next to each other in a rat FAE. (E) Secreting goblet cell in human FAE. (F) Mucus on top of a human FAE, mucus border indicated by black arrow and mucus marked by black star. Bars = 2 µm.</p
Data_Sheet_1_Mucosal affairs: glycosylation and expression changes of gill goblet cells and mucins in a fish–polyopisthocotylidan interaction.PDF [Dataset]
No full text[Introduction]: Secreted mucins are highly O-glycosylated glycoproteins produced by goblet cells in mucosal epithelia. They constitute the protective viscous gel layer overlying the epithelia and are involved in pathogen recognition, adhesion and expulsion. The gill polyopisthocotylidan ectoparasite Sparicotyle chrysophrii, feeds on gilthead seabream (Sparus aurata) blood eliciting severe anemia.[Methods]: Control unexposed and recipient (R) gill samples of gilthead seabream experimentally infected with S. chrysophrii were obtained at six consecutive times (0, 11, 20, 32, 41, and 61 days post-exposure (dpe)). In histological samples, goblet cell numbers and their intensity of lectin labelling was registered. Expression of nine mucin genes (muc2, muc2a, muc2b, muc5a/c, muc4, muc13, muc18, muc19, imuc) and three regulatory factors involved in goblet cell differentiation (hes1, elf3, agr2) was studied by qPCR. In addition, differential expression of glycosyltransferases and glycosidases was analyzed in silico from previously obtained RNAseq datasets of S. chrysophrii-infected gilthead seabream gills with two different infection intensities.[Results and Discussion]: Increased goblet cell differentiation (up-regulated elf3 and agr2) leading to neutral goblet cell hyperplasia on gill lamellae of R fish gills was found from 32 dpe on, when adult parasite stages were first detected. At this time point, acute increased expression of both secreted (muc2a, muc2b, muc5a/c) and membrane-bound mucins (imuc, muc4, muc18) occurred in R gills. Mucins did not acidify during the course of infection, but their glycosylation pattern varied towards more complex glycoconjugates with sialylated, fucosylated and branched structures, according to lectin labelling and the shift of glycosyltransferase expression patterns. Gilthead seabream gill mucosal response against S. chrysophrii involved neutral mucus hypersecretion, which could contribute to worm expulsion and facilitate gas exchange to counterbalance parasite-induced hypoxia. Stress induced by the sparicotylosis condition seems to lead to changes in glycosylation characteristic of more structurally complex mucins.Peer reviewe
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