79 research outputs found
O mito em jogo : um estudo do romance a obscena senhora D de Hilda Hilst
No full textDissertação (mestrado) - Universidade Federal de Santa Catarina. Centro de Comunicação e ExpressãoO presente trabalho se propõe a uma abordagem mítica do romance "A Obscena Senhora D", de Hilda Hilst. Para atingir este objetivo, levantamos os componentes míticos em relação à personagem com o cosmos. Constatamos a presença do mito na própria constituição ritual da obra
Ambient vibrations for the assessment of the strengthening intervention of a masonry barrel vault
No full textVibration-based structural monitoring is a fundamental tool to assess the conditions of existing structures, in their real operating state. In particular, as concerns masonry buildings, although a large part of the Italian and European historical heritage is composed of vaulted structures, comparatively few papers in the literature are targeted to the study of the dynamic behavior of vaulted systems. The present work focuses on the application of vibration-based structural monitoring to a barrel vault of the Bussi Castle, located in Pescara, Italy, which suffered some damages as a consequence of the 2009 L’Aquila earthquake. Ambient vibration tests were carried out in the damaged state and after repair and strengthening of the vault for evaluating its dynamic behavior in both states. In either conditions, the modal parameters of the vault were identified using operational modal analysis techniques. The comparison of the modes in the two states, carried out with Modal Assurance Criterion index, clearly indicates an enhancement of dynamic behavior of the consolidated vault. In particular, a new index named Modal Symmetry Index is introduced. The index is based on a proper ratio between mode shapes to evaluate the improvement of the structural symmetry after the restoration interventions. The results confirm the effectiveness of the devised index to evaluate the strengthening interventions and, the potential of the structural monitoring to control the behavior of damaged vaulted masonry systems. © The Author(s) 2020
The TRIM family protein KAP1 inhibits HIV-1 integration.
No full textThe integration of viral cDNA into the host genome is a critical step in the life cycle of HIV-1. This step is catalyzed by integrase (IN), a viral enzyme that is positively regulated by acetylation via the cellular histone acetyl transferase (HAT) p300. To investigate the relevance of IN acetylation, we searched for cellular proteins that selectively bind acetylated IN and identified KAP1, a protein belonging to the TRIM family of antiviral proteins. KAP1 binds acetylated IN and induces its deacetylation through the formation of a protein complex which includes the deacetylase HDAC1. Modulation of intracellular KAP1 levels in different cell types including T cells, the primary HIV-1 target, revealed that KAP1 curtails viral infectivity by selectively affecting HIV-1 integration. This study identifies KAP1 as a cellular factor restricting HIV-1 infection and underscores the relevance of IN acetylation as a crucial step in the viral infectious cycle
Thymic sensitivity to hypoxic condition in young and old rats. Age-dependent expression of NF-kappaB.
No full textEffects of senescent secretory phenotype acquisition on human retinal pigment epithelial stem cells
No full textDamage assessment of a cloister vault
No full textVibration-based damage identification methods are fundamental tools for the condition assessment of historical constructions prone to earthquakes. However, despite the substantial advances in the field, several issues must still be deepened to broaden the application range of such tools and to assert their effectiveness. This is particularly true for the vaulted systems considering their modal characteristics. This study deals with the damage detection of a cloister vault of the Middle Age castle sited in Bussi sul Tirino (Abruzzo, Italy), an area of moderate seismicity, which was significantly damaged by an earthquake in 2009. Due to the impossibility to have experimental measures related to the non-damaged initial state, an a priori numerical model of the undamaged vault is set up using the geometrical and mechanical properties measured on site. Ambient noise measurements were then carried out to identify the dynamic behaviour of the cloister vault in the current situation, where seismic damage is present. The a priori model is then updated on the basis of the modal parameters identified by the ambient noise measurements. The differences between the a priori and updated models are analysed to identify the presence of damage. The results found allow for discriminating the real structural damage suffered by the vault
Interferon-mediated intracellular signalling. Modulation of different phospholipase activities in Burkitt lymphoma cells.
No full textThe effect of interferon-alpha on Daudi lymphoma cells either sensitive or resistant to the action of this cytokine has been analysed in terms of phospholipase C (PLC) and D (PLD) activities. Results have shown a combined modulation of PIP2-specific phospholipase C and phospholipase D. In particular, a decreased activity of PIP2-specific PLC has been found, concomitant to a PLD-mediated phosphatidylcholine hydrolysis, suggesting that the intracellular signalling activated by interferon in Daudi cells involves a phospholipase D/phosphohydrolase pathway
Supplemental Material - Recommendations for the Management of Incidental Musculoskeletal Findings on MRI and CT
No full textSupplemental Material for Recommendations for the Management of Incidental Musculoskeletal Findings on MRI and CT by Gina Di Primio, Gordon J. Boyd, Christopher I. Fung, Casey Hurrell, Gary L. Brahm, Jeffery R. Bird, Steven J. Co and Iain D. C. Kirkpatrick in Canadian Association of Radiologists Journal</p
On energy, wealth and development
No full textSIGLEAvailable from Bibliothek des Instituts fuer Weltwirtschaft, ZBW, Duesternbrook Weg 120, D-24105 Kiel C 143778 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman
GCN5-dependent acetylation of HIV-1 integrase enhances viral integration
No full textAbstract Background An essential event during the replication cycle of HIV-1 is the integration of the reverse transcribed viral DNA into the host cellular genome. Our former report revealed that HIV-1 integrase (IN), the enzyme that catalyzes the integration reaction, is positively regulated by acetylation mediated by the histone acetyltransferase (HAT) p300. Results In this study we demonstrate that another cellular HAT, GCN5, acetylates IN leading to enhanced 3'-end processing and strand transfer activities. GCN5 participates in the integration step of HIV-1 replication cycle as demonstrated by the reduced infectivity, due to inefficient provirus formation, in GCN5 knockdown cells. Within the C-terminal domain of IN, four lysines (K258, K264, K266, and K273) are targeted by GCN5 acetylation, three of which (K264, K266, and K273) are also modified by p300. Replication analysis of HIV-1 clones carrying substitutions at the IN lysines acetylated by both GCN5 and p300, or exclusively by GCN5, demonstrated that these residues are required for efficient viral integration. In addition, a comparative analysis of the replication efficiencies of the IN triple- and quadruple-mutant viruses revealed that even though the lysines targeted by both GCN5 and p300 are required for efficient virus integration, the residue exclusively modified by GCN5 (K258) does not affect this process. Conclusions The results presented here further demonstrate the relevance of IN post-translational modification by acetylation, which results from the catalytic activities of multiple HATs during the viral replication cycle. Finally, this study contributes to clarifying the recent debate raised on the role of IN acetylated lysines during HIV-1 infection.</p
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