60 research outputs found
Evaluation of two detergent-disinfectants and a detergent on a Klebsiella pneumoniae biofilm formed within Tygon tubes
No full textInternational audienceBackground: Transmission of infections via contaminated endoscopes is a common problem. Manual cleaning, using at least a detergent, is an important step in endoscope processing and should be performed as soon as possible to avoid drying of organic residues that might interfere with high-level disinfection and promote biofilm formation. Aim: To assess the efficacy of two detergent-disinfectants, enzymatic and non-enzymatic, and of an enzymatic detergent used during the manual cleaning against a Klebsiella pneumoniae biofilm. Methods: A 24 h biofilm statically formed in a Tygon tube was exposed to detergentdisinfectants at 20 C and 35 C for 10 mn, and to enzymatic detergent at 45 C for 60 mn. The logarithmic reduction in bacteria in the Tygon tube and the number of bacteria in the product supernatant were calculated. Findings: Biofilm formation was reproducible between assays. After exposure to detergent-disinfectants, the logarithmic reduction was between 6.32 and 6.71 log 10 cfu/ cm 2 in the Tygon tubes. No bacteria were found in their supernatants. Results in the detergent-disinfectant group were not affected by the exposure temperature or the addition of enzymes. No decrease in the bacterial load was observed in the Tygon tubes after exposure to the enzymatic detergent. Bacteria were found in its supernatant. Conclusion: These results show the importance of the choice of products used during the manual cleaning phase. They also show the potential benefit of combining detergent and disinfectant activity to decrease the bacterial load during the manual cleaning step of endoscope processing.</div
Évaluation de l'efficacité de deux détergents-désinfectants et d'un détergent sur un biofilm de Klebsiella pneumoniae constitué de manière statique au sein de tubes de Tygon
No full textBackground and objectives of the study: endoscopes can be vectors for the transmission of pathogens and can be the cause of nosocomial infection outbreaks. Cleaning and High-level disinfection (HLD) of endoscopes are performed after each use. Manual cleaning is an important step in HLD. It prevents the formation of biofilm and should be performed with a product have at least a detergent property. The objective of this study was to evaluate the effectiveness of two detergent-disinfectants and an enzymatic detergent used during the manual cleaning phase of endoscope on a Klebsiella pneumoniae biofilm.Materials and methods: three technical and biological replicates assays were performed on a 24-hour biofilm was statically constituted in Tygon tubes. A bacterial count was performed on the Tygon and the bacterial logarithmic reduction and logarithmic ratio were calculated after exposure detergent-disinfectants at 20°C and 35°C, and enzymatic detergent at 45°C to the biofilm. Subsequently, a count of viable bacteria in the supernatant of the products was performed.Results: biofilm production was homogeneous between the assays. The bacterial count was below the detection limit on Tygons after exposure to detergent-disinfectants and the log reduction was between 6.32 and 6.1 Log10 CFU/cm2. No viable bacteria were found in their supernatants. No impact of the exposure temperature was found on the detergent-disinfectant group. On the contrary, no decrease of the bacterial load was observed on the Tygon after exposure to the enzymatic detergent and viable bacteria were found in the supernatant.Conclusions: our results demonstrate the importance of the choice of products used during the manual cleaning phase. They also show the interest of combining a detergent and disinfectant activity to decrease the bacterial load during the manual cleaning phase.Contexte et objectifs de l’étude : les endoscopes peuvent être des vecteurs de transmission d'agents pathogènes et être à l'origine d'épidémie d'infection nosocomiale. Ils sont soumis à un nettoyage et une désinfection de haut niveau (DHN) après chaque utilisation. Le nettoyage manuel est une étape importante de la DHN. Il permet d'éviter la formation de biofilm et doit être effectué avec des produits ayant une action détergente. L'objectif de cette étude était d'évaluer l'efficacité de deux détergents-désinfectants et d'un détergent enzymatique, utilisés lors du nettoyage manuel des endoscopes sur un biofilm de Klebsiella pneumoniae.Matériel et méthode : des tests en Triplicat technique et biologique ont été effectués sur un biofilm de 24 heures constitué de manière statique au sein de tubes de Tygon. Après exposition du biofilm aux détergents-désinfectants à 20°C et 35°C, et au détergent enzymatique à 45°C, un compte des bactéries viables a été effectué sur le Tygon, et la réduction logarithmique ainsi que le ratio logarithmique de la biomasse bactérienne ont été calculés. Par la suite, une numération des bactéries viables dans le surnageant des produits a été effectuée.Résultats : la formation du biofilm était homogène entre les essais. Le compte bactérien des Tygons après exposition aux détergents-désinfectants était en dessous du seuil de détection, la réduction logarithmique était entre 6.32 et 6.1 Log10 UFC/cm2. Aucune bactérie viable n'était retrouvée dans leurs surnageants. La température d'exposition n'avait pas d'influence sur l'efficacité des détergents-désinfectants. Aucune diminution de la charge bactérienne n'était retrouvée après exposition au détergent enzymatique et des bactéries viables ont été retrouvées dans le surnageant.Conclusions : nos résultats montrent l'importance du choix des produits utilisés lors de la phase de nettoyage manuelle et l'intérêt de combiner une activité détergente et désinfectante pour la diminution de la charge bactérienne
Particularités épidémiologiques et prévention des infections nosocomiales virales
No full textInternational audienc
Investigation d’une épidémie hospitalière et règles de prise en charge des cas suspects de rougeole ou d’infection invasive à méningocoque en établissement de soins
No full textInternational audienceLes épidémies de maladies infectieuses en établissement de soins doivent être précocement détectées et contrôlées. La bonne connaissance des étapes de l’enquête d’investigation, des outils de cette enquête et du rôle des acteurs hospitaliers permet d’organiser une prise en charge efficace de l’épidémie. L’épidémie est également une opportunité formidable pour identifier les brèches du système de santé, et mettre en place des actions de formation et d’éducation
Klebsiella pneumoniae survival and regrowth in endoscope channel biofilm exposed to glutaraldehyde and desiccation
No full textInternational audienc
L'esprit nouveau de la jurisprudence en matière de péremption d'instance
No full textInternational audience(Civ. 2e, 10 oct. 2024, no 22-12.882 et no 22-20.384, deux arrêts, publiés au Bulletin ; D. actu. 6 nov. 2024, obs. M. Plissonnier ; D. 2024. 1781 ; ibid. 2025. 652, no 6, chron. C. Dudit ; RDSS 2025. 170, note N. Jacob ; Civ. 2e, 27 mars 2025, no 22-15.464 et no 22-20.067, deux arrêts, FS-B ; D. actu. 15 avr. 2025, obs. R. Raine ; AJDI 2025. 383 ; RTD com. 2025. 308, obs. D. Mouralis ; JCP 2025. 430, note G. Deharo ; ibid. 652, no 2, obs. L. Veyre ; JCP 2025. 777, note N. Fricero ; Procédures 2025, no 108, note R. Laffly ; Dr. fam. 2025, no 112, obs. V. Egéa ; Gaz. Pal. 15 avr. 2025, p. 43, obs. S. Amrani-Mekki ; ibid. 22 juill. 2025, obs. M. Plissonnier ; Civ. 2e, 14 nov. 2024, no 22-23.185, publié au Bulletin ; D. actu. 29 nov. 2024, obs. M. Barba ; D. 2025. 505, obs. N. Fricero ; Gaz. Pal. 14 janv. 2025, p. 68, obs. M. Plissonnier ; BJT 2025/01, p. 38, obs. X. Aumeran
What is the viable microbiome of the healthcare toilet?
No full textBackgroundFlushing water toilets in hospitals cater for staff and patients, including disabled and unisex facilities. The recent introduction of unisex toilets has highlighted potential microbiological risks between different bathroom facilities.AimThis project sought to establish the cultivable microbiome in all types of healthcare toilet.MethodsSystematic sampling was performed using quantitative and qualitative microbiology within a multi-site longitudinal study. Hand-touch sites, low sites (floors) and high sites (ceilings) in male/female staff and patient, unisex and disabled facilities were screened in three hospitals. Aerobic colony count (ACC) was determined alongside identification of key pathogens (Staphylococcus aureus, Enterobacterales, enterococci, stenotrophomonas, acinetobacter and fungi). Bacterial pathogens were tested for antimicrobial susceptibilities.FindingsExpected environmental and skin flora were present at all sites. Bioburden was heaviest on floors, closely followed by ceilings. Hand-touch sites were cleanest (12 cfu/cm2) occurred in unisex toilets, followed by male toilets. Flora at high sites was almost identical to that on floors. Bacterial pathogens, including resistant bacteria, were concentrated on patient floors, with 80% isolated alongside ACC>2.5 cfu/cm2. Seven of eight Stenotrophomonas maltophilia were recovered from gender-neutral toilets. S. aureus predominated on floors and hand-touch sites in male staff and disabled toilets (P=0.02 and P<0.001, respectively).ConclusionCleaning frequencies should be modelled against higher bioburden (unisex) and risk of pathogens (patient toilets). Similar flora on floors and ceilings suggested aerosolization during flushing. The data provides evidence to inform bathroom design, designation and decontamination practices
Ressenti du port du masque systématique par les professionnels de santé non vaccinés pendant la période épidémique grippale
No full textEffects of Ser130Gly and Asp240Lys Substitutions in Extended-Spectrum -Lactamase CTX-M-9
No full textInternational audienceIn CTX-M-9 extended-spectrum β-lactamases (ESBLs), an S130G mutation induced a 40- to 650-fold increase in 50% inhibitory concentrations but decreased hydrolytic activity against cefotaxime. A D240K mutation did not modify enzymatic efficiency against ceftazidime. Residue K240 could interact with Q270 and therefore not with ceftazidime, in contrast with what was observed with certain TEM/SHV-type ESBLs
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