87 research outputs found
Turkish Adaptation of the Berkeley Parenting Self-Efficacy Scale Revised (BPSE-R)
No full textThe purpose of this study is to adapt the Berkeley Parenting Self-Efficacy Scale Revised (BPSE-R) to Turkish. The scale is composed of two subscales, parental strategies and child outcomes, and includes 18 items. In the process of adapting the scale and examining its reliability and validity, 354 mothers of preschool children were selected through convenience sampling. After translation-and back translation procedures, the BPSE-R was evaluated by field experts, and the scale items were found to be consistent. Confirmatory factor analysis showed that the scale has a two-factor structure similar to the original scale. The fit indices obtained for this two-factor structure were found to be at a good level. The total score Cronbach's alpha internal consistency coefficient was calculated as 0.91, and the test-retest reliability coefficient was calculated as 0.67. Findings demonstrated that the Turkish version of the BPSE-R has adequate psychometric characteristics and can be used in determining the self-efficacy of parents in Turkey with preschool-aged children. The short duration in which the BPSE-R can be applied and its high reliability make it convenient for use in research
PENGARUH LAMA PENYIMPANAN TERHADAP KUALITAS SEMEN AYAM KAMPUNG UNGGUL BALITNAK (KUB-1) YANG DI ENCERKAN DENGAN BELSTVILLE POULTRY SEMEN EXTENDER (BPSE)
No full textPENGARUH LAMA PENYIMPANAN TERHADAP KUALITAS SEMEN AYAM KAMPUNG UNGGUL BALITNAK (KUB-1) YANG DIENCERKAPN DENGAN BELTSVILLE POULTRY SEMEN EXTENDER (BPSE)
Apriadi, di bawah bimbingan
Dr. Ir. Masrizal, MS dan Dr. Rusfidra, S.Pt, MP
Bagian Teknologi Produksi Ternak Fakultas Peternakan
Universitas Andalas Padang, 2022
ABSTRAK
Penelitian ini bertujuan untuk mengetahui waktu penyimpanan yang optimal pada semen Ayam Kampung Unggul Balitnak (KUB-1) menggunakan pengencer BPSE yang diukur berdasarkan kualitas spermatozoa Ayam KUB-1 meliputi motilitas, persentase hidup dan abnormalitas. Penelitian ini menggunakan metode eksperimen dengan 4 perlakuan yaitu penyimpanan 0 jam, 3 jam, 5 jam dan 7 jam dengan 4 kali pengambilan semen sebagai ulangan. Ayam KUB-1 yang dipakai rata-rata sudah berumur 1 tahun lebih. Sperma diambil dengan metode massage dari semua ayam yang berjumlah 4 ekor, lalu semen dibagi menjadi 4 bagian dan diencerkan dengan BPSE sampai konsentrasi 800 jt/ml, selanjutnya dilakukan penyimpanan semen sesuai dengan perlakuan pada suhu 40C. Masing-masing perlakuan diamati persentase motilitas, persentase hidup, persentase abnormalitas menggunakan mikroskop. Data hasil penelitian diolah dengan analisis of varians menggunakan Rancangan Acak Kelompok (RAK) dan uji lanjut dengan duncan’s multiple range test (DMRT). Hasil penelitian menunjukkan bahwa lama penyimpanan semen ayam KUB yang diencerkan dengan BPSE memberikan pengaruh sangat nyata (P0,05) terhadap persentase abnormalitas spermatozoa. Penelitian ini menunjukkan pula bahwa kualitas semen ayam KUB masih layak digunakan untuk IB sampai lama penyimpanan 7 jam.
Kata kunci : Ayam Kampung Unggul Balitnak, penyimpanan, semen, kualitas spermatozoa
Different coordination modes for disulfoxides towards diorganotin(IV) dichlorides. X-ray crystal structures of 1,2-cis-bis-(phenylsulfinyl)ethene (rac-,cis-cbpse) and adducts [{Ph2SnCl2(meso-bpse)}n] and [{n-Bu2SnCl2(pdtd)}2]
No full textThe reactions of meso-1,2-bis(phenylsulfinyl)ethane (meso-bpse) with Ph2SnCl2, 2-phenyl-1,3-dithiane trans-1-trans-3-dioxide (pdtd) with n-Bu2SnCl2 and 1,2-cis-bis-(phenylsulfinyl)ethene (rac-,cis-cbpse) with Ph2SnCl2, in 1:1 molar ratio, yielded [{Ph2SnCl2(meso-bpse)}n], [{n-Bu2SnCl2(pdtd)}2] and [{Ph2SnCl2(rac,cis-cbpse)}x] (x = 2 or n), respectively. All adducts were studied by IR, Mössbauer and 119Sn NMR spectroscopic methods, elemental analysis and single crystal X-ray diffractometry. The X-ray crystal structure of [{Ph2SnCl2(meso-bpse)}n] revealed the occurrence of infinite chains in which the tin(IV) atoms appear in a distorted octahedral geometry with Cl atoms in cis and Ph groups in trans positions. The X-ray crystal structure of [{n-Bu2SnCl2(pdtd)}2] revealed discrete centrosymmetric dimeric species in which the tin(IV) atoms possess a distorted octahedral geometry with bridging disulfoxides in cis and n-butyl moieties in trans positions. The spectroscopic data indicated that the adduct containing the rac,cis-cbpse ligand can be dimeric or polymeric. The X-ray structural analysis of the free rac-,cis-cbpse sulfoxide revealed that the crystals belong to the C2/c space group.As reações de meso-1,2-bis(fenilsulfinil)etano (meso-bpse) com Ph2SnCl2, de 2-fenil-1,3-ditiona trans-1-trans-dióxido (pdtd) com n-Bu2SnCl2 e de 1,2-cis-bis-(phenylsulfinyl)ethene (rac-,cis-cbpse) com Ph2SnCl2, na proporção molar 1:1, levaram à formação de [{Ph2SnCl2(meso-bpse)}n], [{n-Bu2SnCl2(pdtd)}2] e [{Ph2SnCl2(rac,cis-cbpse)}x] (x = 2 ou n), respectivamente. Na investigação das propriedades estruturais dos produtos foram empregadas as espectroscopias de absorção no infravermelho, Mössbauer e RMN de 119Sn, além de análise elementar e difratometria de raios X em monocristal. O estudo de [{Ph2SnCl2(meso-bpse)}n] por difratometria de raios X revelou a ocorrência de um encadeamento infinito no qual os átomos de tin(IV) apresentam uma geometria octaédrica distorcida com os átomos de Cl em posições cis e os grupos Ph em trans. A estrutura cristalina de [{n-Bu2SnCl2(pdtd)}2] revelou a presença de espécies diméricas centrossimétricas nas quais os átomos de tin(IV) possuem geometria octaédrica distorcida com dissulfóxidos em ponte ocupando posições cis e grupos n-butila ocupando posições trans. Os dados espectroscópicos indicaram que o produto contendo o ligante rac,cis-cbpse pode ser dimérico ou polimérico. O estudo por difratometria de raios X do sulfóxido rac-,cis-cbpse livre revelou que os cristais pertencem ao grupo espacial C2/c.CNPqFAPESPFINE
Functional Protection of Exfoliated Black Phosphorus by Noncovalent Modification with Anthraquinone
No full textFew and monolayer black phosphorus
(phosphorene) is currently an
intensively researched material. Shear exfoliated black phosphorus
(BPSE) nanosheets were functionalized with the redox active
antraquinone (AQ) that can provide additional charge storage capacity.
The noncovalent interaction of BP with AQ occurs due to van der Waals
interactions. X-ray photoelectron spectroscopy results show that AQ
coverage of BPSE nanosheets led to a stabilization against
BPSE degradation. Electrochemistry of the BPSE-AQ shows that AQ is stably anchored onto BPSE and exhibits
redox peaks stable for more than 100 cycles. The surface coverage
by AQ on BPSE is estimated to be 1.25 nmol AQ/mg BP and
electron-transfer rate constant (kET)
of 33 s–1. Furthermore, the proposed modification
greatly increases the gravimetric capacitance of BPSE-AQ
with respect to the starting BPbulk. Such coating of BP
not only protects BP from degradation but also brings electroactive
functionality to this two-dimensionally layered material
Proton (H+) transport properties of CMC–PVA blended polymer solid electrolyte doped with NH4NO3
No full textThis present work investigated the proton (H+) conduction behavior of the blended polymer solid electrolyte (BPSE) derived from carboxymethyl cellulose (CMC) and polyvinyl alcohol (PVA) that was doped with ammonium nitrate (NH4NO3). The properties of this CMC-PVA-AN BPSE were evaluated using Fourier transform infrared spectroscopy (FTIR), transference number measurement (TNM), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray diffraction (XRD), scanning electron microscopy (SEM), and electrical impedance spectroscopy (EIS). We found that doping (NH4NO3) improved the chemical and thermal stability of the CMC-PVA BPSE. The highest ionic conductivity (~10−3 S/cm) of the BPSE at ambient temperature was achieved with a CMC:PVA:NH4NO3 composition of 56:14:30 wt%. This was due to the interplay of segmental motion between the CMC and PVA and also the H+ hopping mechanism as revealed by FTIR. XRD and morphology analysis showed that the peak intensity decreased which implied an increase in its amorphous nature. Based on the transport properties, the CMC-PVA-AN BPSE conduction mechanism was governed by number of ions, ionic mobility and also free ions diffusion coefficient. The proton transference number (= 0.42) in the present study indicated that the charge transport in the BPSE was predominantly due to the H+ carrier conduction
When do sphenoidal electrodes yield additional data to that obtained with antero-temporal electrodes?
No full textThe advantage of using sphenoidal (SE) over antero-temporal electrodes (ATE) remains controversial among epileptologists. Yet, in a recently published study of 17 patients with seizures of antero-temporal origin (Kanner et al., 1995), we demonstrated that SE placed under fluoroscopic guidance (FPSE), in order to insure that their recording tips are positioned immediately below the foramen ovale (FO), yielded a significant advantage over SE placed with the standard blind method of insertion (BPSE), in both interictal and ictal recordings. This study was done to test the following hypothesis: FPSE advantage over BPSE and ATE resides in the recording of epileptiform activity with a restricted electric field. We compared spike voltages at FPSE, BPSE and ATE in sets of 5 randomly selected spikes per interictal focus, recorded in the course of separate monitoring studies with BPSE and FPSE. We represented the voltage differences as ratios, VATE/FPSE and VATE/BPSE and calculated a mean ratio for each spike set. The spikes' voltage was almost identical at BPSE and at ATE (mean VATE/BPSE = 0.94), while it was significantly higher at FPSE than at ATE (mean VATE/FPSE = 0.66; P < 0.001, t test). A significantly narrower electric field contour was found among interictal foci in which FPSE yielded additional data during interictal (P < 0.001) and ictal (P = 0.016) recordings. Conversely, VATE/FPSE did not differ from VATE/BPSE among interictal foci where FPSE failed to yield any advantage over BPSE in either interictal (P = 0.240), or ictal (P = 0.311) recordings. These findings prove that SE yield additional localizing data when recording epileptiform activity with a restricted field, provided that its recording tip is positioned below the FO. When distant from FO, SE can be expected to yield comparable data to that obtained with ATE
Fertilizing capacity and morphology of fowl and turkey spermatozoa in hypotonic extender
No full textThe effects of graduated increments of hypotonic Beltsville Poultry Semen Extender (BPSE) on fowl and turkey sperm morphology, motility, percentage deadand fertility were examined. The morphology of fowl and turkey spermatozoa wasadversely affected by decreases in BPSE osmolarity; a reduction of osmolarity ofBPSE to below 200 and 140 mosmol adversely affected the fertility of fowl andturkey spermatozoa, respectively. The addition of Ficoll to the extender alloweddirect observation of the effects of hypotonic BPSE on the sperm plasmalemma.Plasmalemmal swelling, due to the uptake of water, was noted initially at themidpiece. Such plasmalemmal swelling may predispose spermatozoa to furtherosmotic-induced damage
Evaluation of two diluents for the storage of fresh and cryopreserved semen of Harris hawk (Parabuteo unicinctus)
No full textSeminal storage, both fresh and cryopreserved, has contributed to the reproduction of wild birds in captivity, however, a method of predicting the fertilization capacity is needed. Indicators of the sperm acrosome reaction (AR) have been associated with its fertilization capacity. Therefore, the aim of this study was to evaluate the in vitro AR of fresh and cryopreserved Harris hawk sperm using Beltsville Poultry Semen Extender (BPSE) and Lake as diluents. 64 ejaculates were obtained and examined fresh and after thawing. The sperm basic evaluation for each ejaculate was made using eosin-nigrosin, while the ability of AR was assessed by coincubation with perivitelline layer (PVL). Sperm motility of fresh semen was higher (P<0.05) in fresh semen in BPSE (37.9±1.7) than in Lake (30.9±1.7) diluent. However, the motility decreased (P<0.05) in both diluents after thawing. For fresh semen, the percentage of sperm that underwent an AR without incubation with PVL was higher (P<0.05) with BPSE (14.1±1.7) than with Lake (6.8±2.5) diluent, however, AR was similar between tow diluents (P>0.05) after thawing. The percentage of sperm that underwent an AR when incubated with PVL post thawing in Lake (45.4±2.7) was lower (P<0.05) than that of fresh semen (55.3±3.1), whereas there were no differences (P>0.05) with BPSE. It is concluded that Lake diluent was more efficient for fresh seminal storage, while BPSE diluent was more efficient for seminal cryopreservation in Harris hawk
Self-assembled monolayers of aromatic selenolates on gold and silver.
No full textSelf-assembled monolayers (SAMs) formed from bis(biphenyl-4-yl) diselenide (BBPDSe) on An(111) and Ag(111) substrates have been characterized by high-resolution X-ray photoelectron spectroscopy, near-edge X-ray absorption fine structure spectroscopy, infrared reflection absorption spectroscopy, water contact angle measurements, and scanning tunneling microscopy (STM). BBPDSe was found to form contamination-free, densely packed, and well-ordered biphenyl selenolate (BPSe) SAMs on both An and Ag. Spectroscopic data suggest very similar packing density, orientational order, and molecular inclination in BPSe/Au and BPSe/ Ag. STM data give a, similar intermolecular spacing of 5.3 +/- 0.4 angstrom on both Au and Ag but exhibit differences in the exact arrangement of the BPSe molecules on these two substrates, with the (2 root 3x root 3)R30 degrees and (root 3 x root 3)R30 degrees unit cells on Au and Ag, respectively. There is strong evidence for adsorbate-mediated substrate restructuring in the case of Au, whereas no clear statement on this issue can be made in the case of Ag. The film quality of the BPSe SAMs is superior to their thiol analogues, which is presumably related to a better ability of the selenolates to adjust the surface lattice of the substrate to the most favorable 2D arrangement of the adsorbate molecules. This suggests that aromatic selenolates represent an attractive alternative to the respective thiols.</p
Evaluation of two diluents for the storage of fresh and cryopreserved semen of Harris hawk (Parabuteo unicinctus)
No full textEl almacenamiento seminal in vitro ha contribuido a la reproducción de aves silvestres en cautiverio. Sin embargo es necesario predecir su capacidad fertilizante. Los indicadores de reacción acrosomal (RA) espermática se han relacionado con su capacidad fertilizante. Por lo anterior, el objetivo de este trabajo fue evaluar la capacidad de reacción acrosomal in vitro en espermatozoides de halcón Harris, conservados en fresco y criopreservados. Se obtuvieron 64 eyaculados, los cuales fueron evaluados en fresco y posdescongelación, 32 se diluyeron con medio Beltsville Poultry Semen Extender (BPSE) y 32 utilizando medio Lake. Se evaluaron 64 eyaculados en fresco y posdescongelación; para la evaluación espermática básica se utilizó la tinción de eosina-nigrosina, y capacidad de RA fue mediante su inducción con membrana perivitelina (MPV). La movilidad espermática en semen fresco fue mayor (P<0,05) cuando se usó el medio BPSE (37,9±1,7) en comparación con el medio Lake (30,9±1,7). Sin embargo, la movilidad en ambos diluyentes disminuyó (P<0,05) posdescongelación. En semen fresco, el porcentaje de RA sin incubar con MPV fue mayor (P<0,05) con medio BPSE (14,1±1,7) al porcentaje con medio Lake (6,8±2,5). En semen descongelado los promedios de RA fueron similares (P>0,05). El porcentaje de espermatozoides con RA en el medio Lake, incubados con MPV posdescongelación (45,4±2,7), fue menor (P<0,05), comparado en fresco (55,3±3,1). Se concluye que el diluyente Lake fue más eficaz para la conservación en fresco y el diluyente BPSE para la criopreservación seminal de halcón Harris.Seminal storage, both fresh and cryopreserved, has contributed to the reproduction of wild birds in captivity, however, a method of predicting the fertilization capacity is needed. Indicators of the sperm acrosome reaction (AR) have been associated with its fertilization capacity. Therefore, the aim of this study was to evaluate the in vitro AR of fresh and cryopreserved Harris hawk sperm using Beltsville Poultry Semen Extender (BPSE) and Lake as diluents. 64 ejaculates were obtained and examined fresh and after thawing. The sperm basic evaluation for each ejaculate was made using eosin-nigrosin, while the ability of AR was assessed by co-incubation with perivitelline layer (PVL). Sperm motility of fresh semen was higher (P<0.05) in fresh semen in BPSE (37.9±1.7) than in Lake (30.9±1.7) diluent. However, the motility decreased (P<0.05) in both diluents after thawing. For fresh semen, the percentage of sperm that underwent an AR without incubation with PVL was higher (P<0.05) with BPSE (14.1±1.7) than with Lake (6.8±2.5) diluent, however, AR was similar between tow diluents (P>0.05) after thawing. The percentage of sperm that underwent an AR when incubated with PVL post thawing in Lake (45.4±2.7) was lower (P<0.05) than that of fresh semen (55.3±3.1), whereas there were no differences (P>0.05) with BPSE. It is concluded that Lake diluent was more efficient for fresh seminal storage, while BPSE diluent was more efficient for seminal cryopreservation in Harris hawk
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