1,354,142 research outputs found

    Black lipid membranes studied by x-ray phase contrast imaging

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    Softcover, 140 S.: 40,00 €Softcover, 17x24Membranes fulfill many different functions inside a cell and have consequently been in the focus of biophysical studies for several decades. High resolution structure analysis of model membrane systems by x-ray reflectivity is typically performed using stabilizing solid supports, which have shown to strongly interact with the membranes and can severly affect the membrane`s functionality. To circumvent these restrictions free-standing lipid model membranes, also called Black Lipid Membranes (BLMs), have been used in this work towards a novel type of structural assay based on x-ray phase contrast imaging. Bulged lipid bilayers fully immersed in buffer solution have been visualized by a transmitted plane or spherical x-ray wave front in the Fresnel regime after free space propagation. The development of the experimental system and the image analysis enables a quantitative investigation of the membrane`s structural properties in a localized membrane patch. In future this technique can be used to study the structure of membranes and more generally ultrathin films in functional and physiological environments and under out-of-equilibrium conditions

    Hard x-ray phase contrast imaging of black lipid membranes

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    We report hard x-ray phase contrast imaging of black lipid membranes, freely suspended over a micromachined aperture in an aqueous solution. Biomolecular and organic substances can thus be probed in hydrated environments by parallel beam propagation imaging, using coherent multi-kilo-electronvolt x-ray radiation. The width of the thinning film can be resolved from analysis of the intensity fringes in the Fresnel diffraction regime down to about 200 nm. The thinning process, in which solvent is expelled from the space in between two opposing monolayers, is monitored, and the domain walls between coexisting domains of swollen and thinned membrane patches are characterized

    Bombina bombina ZMH A05383, unstained CT volume

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    Species: Bombina bombina Collection number: ZMH A05383 Institution of origin: Zoologisches Museum Hamburg Data: CT volume of unstained specimen Coverage: full specimen Scanner: YXLON FF35 CT Scanning paramters: 100 kV; 120 µA; no filter Voxel size: 22.7455 µm Dataset used in: Engelkes, K., Helfsgott, J., Hammel, J. U., Büsse, S., Kleinteich, T., Beerlink, A., Gorb, S. N., Haas, A. (2019) Measurement error in μCT-based three-dimensional geometric morphometrics introduced by surface generation and landmark data acquisition. Journal of Anatomy 235(2): 357–378. https://doi.org/10.1111/joa.12999. Engelkes, K., Kath, L., Kleinteich, T., Hammel, J. U., Beerlink, A., Haas, A. (2020) Ecomorphology of the pectoral girdle in anurans (Amphibia, Anura): Shape diversity and biomechanical considerations. Ecology and Evolution 10(20): 11467–11487. https://doi.org/10.1002/ece3.6784

    Rana temporaria ZMH A11310, unstained CT volume

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    Species: Rana temporaria Collection number: ZMH A11310 Institution of origin: Zoologisches Museum Hamburg Data: CT volume of unstained specimen Coverage: full specimen Scanner: YXLON FF35 CT Scanning paramters: 100 kV; 55 µA; filter: no filter Voxel size: 26.1784 µmDataset used in: Engelkes, K., Kath, L., Kleinteich, T., Hammel, J. U., Beerlink, A., Haas, A. (2020) Ecomorphology of the pectoral girdle in anurans (Amphibia, Anura): Shape diversity and biomechanical considerations. Ecology and Evolution 10(20): 11467–11487. https://doi.org/10.1002/ece3.6784

    Bombina bombina ZMH A05110, unstained CT volume

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    Species: Bombina bombina Collection number: ZMH A05110 Institution of origin: Zoologisches Museum Hamburg Data: CT volume of unstained specimen Coverage: full specimen Scanner: YXLON FF35 CT Scanning paramters: 100 kV; 120 µA; filter: no filter Voxel size: 22.7455 µm Dataset used in: Engelkes, K., Helfsgott, J., Hammel, J. U., Büsse, S., Kleinteich, T., Beerlink, A., Gorb, S. N., Haas, A. (2019) Measurement error in μCT-based three-dimensional geometric morphometrics introduced by surface generation and landmark data acquisition. Journal of Anatomy 235(2): 357–378. https://doi.org/10.1111/joa.12999. Engelkes, K., Kath, L., Kleinteich, T., Hammel, J. U., Beerlink, A., Haas, A. (2020) Ecomorphology of the pectoral girdle in anurans (Amphibia, Anura): Shape diversity and biomechanical considerations. Ecology and Evolution 10(20): 11467–11487. https://doi.org/10.1002/ece3.6784

    Bombina bombina ZMH A05617, unstained CT volume

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    Species: Bombina bombina Collection number:ZMH A05617 Institution of origin: Zoologisches Museum Hamburg Data: CT volume of unstained specimen Coverage: full specimen Scanner: YXLON FF20 CT Scanning paramters: 80 kV; 80 µA; filter: no filter Voxel size: 25.8364 µm Dataset used in: Engelkes, K., Helfsgott, J., Hammel, J. U., Büsse, S., Kleinteich, T., Beerlink, A., Gorb, S. N., Haas, A. (2019) Measurement error in μCT-based three-dimensional geometric morphometrics introduced by surface generation and landmark data acquisition. Journal of Anatomy 235(2): 357–378. https://doi.org/10.1111/joa.12999. Engelkes, K., Kath, L., Kleinteich, T., Hammel, J. U., Beerlink, A., Haas, A. (2020) Ecomorphology of the pectoral girdle in anurans (Amphibia, Anura): Shape diversity and biomechanical considerations. Ecology and Evolution 10(20): 11467–11487. https://doi.org/10.1002/ece3.6784

    Bufo bufo ZMH A04660, unstained CT volume

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    Species: Bufo bufo Collection number: ZMH A04660 Institution of origin: Zoologisches Museum Hamburg Data: CT volume of unstained specimen Coverage: full specimen Scanner: YXLON FF35 CT Scanning paramters: 70 kV; 120 µA; filter: no filter Voxel size: 25.8 µmDataset used in: Engelkes, K., Kath, L., Kleinteich, T., Hammel, J. U., Beerlink, A., Haas, A. (2020) Ecomorphology of the pectoral girdle in anurans (Amphibia, Anura): Shape diversity and biomechanical considerations. Ecology and Evolution 10(20): 11467–11487. https://doi.org/10.1002/ece3.6784

    Bombina orientalis ZMH A05682, unstained CT volume

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    Species: Bombina orientalis Collection number: ZMH A05682 Institution of origin: Zoologisches Museum Hamburg Data: CT volume of unstained specimen Coverage: full specimen Scanner: YXLON FF35 CT Scanning paramters: 100 kV; 120 µA; filter: no filter Voxel size: 22.7454 µm Dataset used in: Engelkes, K., Helfsgott, J., Hammel, J. U., Büsse, S., Kleinteich, T., Beerlink, A., Gorb, S. N., Haas, A. (2019) Measurement error in μCT-based three-dimensional geometric morphometrics introduced by surface generation and landmark data acquisition. Journal of Anatomy 235(2): 357–378. https://doi.org/10.1111/joa.12999. Engelkes, K., Kath, L., Kleinteich, T., Hammel, J. U., Beerlink, A., Haas, A. (2020) Ecomorphology of the pectoral girdle in anurans (Amphibia, Anura): Shape diversity and biomechanical considerations. Ecology and Evolution 10(20): 11467–11487. https://doi.org/10.1002/ece3.6784

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Bombina bombina ZMH A05619, unstained CT volume

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    Species: Bombina bombina Collection number: ZMH A05619 Institution of origin: Zoologisches Museum Hamburg Data: CT volume of unstained specimen Coverage: full specimen Scanner: YXLON FF35 CT Scanning paramters: 100 kV; 120 µA; filter: no filter Voxel size: 22.7454 µm Dataset used in: Engelkes, K., Helfsgott, J., Hammel, J. U., Büsse, S., Kleinteich, T., Beerlink, A., Gorb, S. N., Haas, A. (2019) Measurement error in μCT-based three-dimensional geometric morphometrics introduced by surface generation and landmark data acquisition. Journal of Anatomy 235(2): 357–378. https://doi.org/10.1111/joa.12999
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