1,354,142 research outputs found
Black lipid membranes studied by x-ray phase contrast imaging
Softcover, 140 S.: 40,00 €Softcover, 17x24Membranes fulfill many different functions inside a cell and have consequently been in the focus of biophysical studies for several decades. High resolution structure analysis of model membrane systems by x-ray reflectivity is typically performed using stabilizing solid supports, which have shown to strongly interact with the membranes and can severly affect the membrane`s functionality. To circumvent these restrictions free-standing lipid model membranes, also called Black Lipid Membranes (BLMs), have been used in this work towards a novel type of structural assay based on x-ray phase contrast imaging. Bulged lipid bilayers fully immersed in buffer solution have been visualized by a transmitted plane or spherical x-ray wave front in the Fresnel regime after free space propagation. The development of the experimental system and the image analysis enables a quantitative investigation of the membrane`s structural properties in a localized membrane patch. In future this technique can be used to study the structure of membranes and more generally ultrathin films in functional and physiological environments and under out-of-equilibrium conditions
Hard x-ray phase contrast imaging of black lipid membranes
We report hard x-ray phase contrast imaging of black lipid membranes, freely suspended over a micromachined aperture in an aqueous solution. Biomolecular and organic substances can thus be probed in hydrated environments by parallel beam propagation imaging, using coherent multi-kilo-electronvolt x-ray radiation. The width of the thinning film can be resolved from analysis of the intensity fringes in the Fresnel diffraction regime down to about 200 nm. The thinning process, in which solvent is expelled from the space in between two opposing monolayers, is monitored, and the domain walls between coexisting domains of swollen and thinned membrane patches are characterized
Bombina bombina ZMH A05383, unstained CT volume
Species: Bombina bombina
Collection number: ZMH A05383
Institution of origin: Zoologisches Museum Hamburg
Data: CT volume of unstained specimen
Coverage: full specimen
Scanner: YXLON FF35 CT
Scanning paramters: 100 kV; 120 µA; no filter
Voxel size: 22.7455 µm
Dataset used in:
Engelkes, K., Helfsgott, J., Hammel, J. U., Büsse, S., Kleinteich, T., Beerlink, A., Gorb, S. N., Haas, A. (2019) Measurement error in μCT-based three-dimensional geometric morphometrics introduced by surface generation and landmark data acquisition. Journal of Anatomy 235(2): 357–378. https://doi.org/10.1111/joa.12999.
Engelkes, K., Kath, L., Kleinteich, T., Hammel, J. U., Beerlink, A., Haas, A. (2020) Ecomorphology of the pectoral girdle in anurans (Amphibia, Anura): Shape diversity and biomechanical considerations. Ecology and Evolution 10(20): 11467–11487. https://doi.org/10.1002/ece3.6784
Rana temporaria ZMH A11310, unstained CT volume
Species: Rana temporaria
Collection number: ZMH A11310
Institution of origin: Zoologisches Museum Hamburg
Data: CT volume of unstained specimen
Coverage: full specimen
Scanner: YXLON FF35 CT
Scanning paramters: 100 kV; 55 µA; filter: no filter
Voxel size: 26.1784 µmDataset used in:
Engelkes, K., Kath, L., Kleinteich, T., Hammel, J. U., Beerlink, A., Haas, A. (2020) Ecomorphology of the pectoral girdle in anurans (Amphibia, Anura): Shape diversity and biomechanical considerations. Ecology and Evolution 10(20): 11467–11487. https://doi.org/10.1002/ece3.6784
Bombina bombina ZMH A05110, unstained CT volume
Species: Bombina bombina
Collection number: ZMH A05110
Institution of origin: Zoologisches Museum Hamburg
Data: CT volume of unstained specimen
Coverage: full specimen
Scanner: YXLON FF35 CT
Scanning paramters: 100 kV; 120 µA; filter: no filter
Voxel size: 22.7455 µm
Dataset used in:
Engelkes, K., Helfsgott, J., Hammel, J. U., Büsse, S., Kleinteich, T., Beerlink, A., Gorb, S. N., Haas, A. (2019) Measurement error in μCT-based three-dimensional geometric morphometrics introduced by surface generation and landmark data acquisition. Journal of Anatomy 235(2): 357–378. https://doi.org/10.1111/joa.12999.
Engelkes, K., Kath, L., Kleinteich, T., Hammel, J. U., Beerlink, A., Haas, A. (2020) Ecomorphology of the pectoral girdle in anurans (Amphibia, Anura): Shape diversity and biomechanical considerations. Ecology and Evolution 10(20): 11467–11487. https://doi.org/10.1002/ece3.6784
Bombina bombina ZMH A05617, unstained CT volume
Species: Bombina bombina
Collection number:ZMH A05617
Institution of origin: Zoologisches Museum Hamburg
Data: CT volume of unstained specimen
Coverage: full specimen
Scanner: YXLON FF20 CT
Scanning paramters: 80 kV; 80 µA; filter: no filter
Voxel size: 25.8364 µm
Dataset used in:
Engelkes, K., Helfsgott, J., Hammel, J. U., Büsse, S., Kleinteich, T., Beerlink, A., Gorb, S. N., Haas, A. (2019) Measurement error in μCT-based three-dimensional geometric morphometrics introduced by surface generation and landmark data acquisition. Journal of Anatomy 235(2): 357–378. https://doi.org/10.1111/joa.12999.
Engelkes, K., Kath, L., Kleinteich, T., Hammel, J. U., Beerlink, A., Haas, A. (2020) Ecomorphology of the pectoral girdle in anurans (Amphibia, Anura): Shape diversity and biomechanical considerations. Ecology and Evolution 10(20): 11467–11487. https://doi.org/10.1002/ece3.6784
Bufo bufo ZMH A04660, unstained CT volume
Species: Bufo bufo
Collection number: ZMH A04660
Institution of origin: Zoologisches Museum Hamburg
Data: CT volume of unstained specimen
Coverage: full specimen
Scanner: YXLON FF35 CT
Scanning paramters: 70 kV; 120 µA; filter: no filter
Voxel size: 25.8 µmDataset used in:
Engelkes, K., Kath, L., Kleinteich, T., Hammel, J. U., Beerlink, A., Haas, A. (2020) Ecomorphology of the pectoral girdle in anurans (Amphibia, Anura): Shape diversity and biomechanical considerations. Ecology and Evolution 10(20): 11467–11487. https://doi.org/10.1002/ece3.6784
Bombina orientalis ZMH A05682, unstained CT volume
Species: Bombina orientalis
Collection number: ZMH A05682
Institution of origin: Zoologisches Museum Hamburg
Data: CT volume of unstained specimen
Coverage: full specimen
Scanner: YXLON FF35 CT
Scanning paramters: 100 kV; 120 µA; filter: no filter
Voxel size: 22.7454 µm
Dataset used in:
Engelkes, K., Helfsgott, J., Hammel, J. U., Büsse, S., Kleinteich, T., Beerlink, A., Gorb, S. N., Haas, A. (2019) Measurement error in μCT-based three-dimensional geometric morphometrics introduced by surface generation and landmark data acquisition. Journal of Anatomy 235(2): 357–378. https://doi.org/10.1111/joa.12999.
Engelkes, K., Kath, L., Kleinteich, T., Hammel, J. U., Beerlink, A., Haas, A. (2020) Ecomorphology of the pectoral girdle in anurans (Amphibia, Anura): Shape diversity and biomechanical considerations. Ecology and Evolution 10(20): 11467–11487. https://doi.org/10.1002/ece3.6784
Going Beyond Counting First Authors in Author Co-citation Analysis
The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Bombina bombina ZMH A05619, unstained CT volume
Species: Bombina bombina
Collection number: ZMH A05619
Institution of origin: Zoologisches Museum Hamburg
Data: CT volume of unstained specimen
Coverage: full specimen
Scanner: YXLON FF35 CT
Scanning paramters: 100 kV; 120 µA; filter: no filter
Voxel size: 22.7454 µm
Dataset used in:
Engelkes, K., Helfsgott, J., Hammel, J. U., Büsse, S., Kleinteich, T., Beerlink, A., Gorb, S. N., Haas, A. (2019) Measurement error in μCT-based three-dimensional geometric morphometrics introduced by surface generation and landmark data acquisition. Journal of Anatomy 235(2): 357–378. https://doi.org/10.1111/joa.12999
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