58 research outputs found
Summer Workshop on Physics, Mathematics, and All That Quantum Jazz
No full textThis book is a collection of contributions from a Summer Workshop on Physics, Mathematics, and All That Quantum Jazz . Subjects of the symposium include quantum information theory, quantum annealing, Bose gases, and thermodynamics from a viewpoint of quantum physics. Contributions to this book are prepared in a self-contained manner so that readers with a modest background may understand the subjects
An Approach to Easy Construction of 3D Space and Its Implementation
No full textThis paper presents “easy-three,“ a simplified library for creating 3D spaces based on Three.js. By abstracting complex configurations, the library makes it easy for beginners to design 3D graphics with minimal code. It eliminates the need for dedicated software, requiring only a text editor and web browser. Easy-three is particularly effective in educational settings, where it facilitates interactive and accessible 3D programming lessons. Future developments will focus on expanding features and supporting educational use cases.departmental bulletin pape
Developing a stochastic traffic volume prediction model for public-private partnership projects
No full textPreparation of multilayer graphene sheets and their applications for particle accelerators
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ヒト分泌型アルカリホスファターゼ発現を安定に維持する新規レポーター細胞の樹立
No full textBackground: Transcriptional regulation is a very important and pivotal function in myriad biological responses. Thus, methods to determine transcriptional activity are required in not only basic medical research but also in drug discovery. We established novel reporter constructs using human secreted embryonic alkaline phosphatase (SEAP) and Epstein-Barr virus nuclear antigen (EBNA) 1, which can maintain constructs synchronized to host cell replication. Methods: We established nuclear factor-kappa B (NFkB) or interferon regulatory factor (IRF) driven SEAP expression constructs and then, introduced them into culture cells. Results: The cells maintain reporter constructs for a long period in the culture and produce SEAP into culture supernatant in response to each specific ligand such as lipopolysaccharide (LPS) and interferon- beta. Measuring SEAP with chemiluminescence makes it possible to get high standard dynamic range applying to high-throughput screening in drug discovery in both 96 and 384 well format. We can also use it to determine transcriptional activity in the cells transfected with expression plasmid or treated with various toll-like receptor (TLR) ligands in a concentration-dependent manner and time-dependent manner. Finally, we demonstrated drug screening using a number of natural products library. Conclusion: We for the first time established the two novel reporter cells and validated their quality and accuracy enough to carry out drug screening.長崎大学学位論文 学位記番号:博(医歯薬)甲第1150号 学位授与年月日:平成31年3月20日Author: Riho Kurata, Asuka Kumagai, Xiaofeng Cui, Masamitsu Harada, Jun Nagai, Yasuhiro Yoshida, Kei-ichi Ozaki, Yoshimasa Tanaka, Tomo YonezawaCitation: Current Pharmaceutical Biotechnology, 19(3), pp.224-231; 201
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