64 research outputs found
In Silico Study of Acetogenin Compounds from Soursop (Annona muricata) Leaves as Sodium-Glucose Cotransporter-2 (SGLT2) Inhibitors: Studi In Silico Senyawa Acetogenin dari Daun Sirsak (Annona muricata) Sebagai Inhibitor Sodium-Glucose Cotransporter-2 (SGLT2)
Get PDFAcetogenin derived from soursop (Annona muricata) leaves are known to have antidiabetic and anticancer activities. Nevertheless, there has been no study related to the compounds found in A. muricata leaves, such as acetogenin, as SGLT2 inhibitors. This research aims to investigate the activity of acetogenin compounds as SGLT2 inhibitors while maintaining low selectivity against SGLT1 using molecular docking methods using Molegro Virtual Docker (MVD). Based on the Rerank score, five acetogenin compounds, namely muricin H, annonacin A, annopentocin B, murihexocin C, and corossolone, are predicted to be SGLT2 inhibitors with better selectivity compared to empagliflozin. Among these five compounds, muricin H and corossolone exhibit the most similarity in interaction with amino acid residues in the SGLT2 A-chain compared to empagliflozin. In silico ADMET analysis results indicate that both compounds have absorption, distribution, and metabolism capabilities, similar to empagliflozin. However, it should be noted that both compounds are more toxic, with muricin H predicted to have hepatotoxic properties
Potential of Dietary Flavonoids in The Prevention and Therapy of COVID-19 : Focusing in Mast Cell - Calcium Ion Channel Axis
Get PDFSyndrome Acute Respiratory Syndrome Corona Virus-2 (SARS COV2) is the virus that causes the COVID19 disease and has caused more than 4 million deaths worldwide. This virus infects the host cell through the interaction between the virus’s glycoprotein S molecule with the ACE2 which is the virus receptor, binding, undergoes membrane fusion and enters the cell and replicates in it. Currently, several strategies used in developing anti-viral compounds are targeting compounds that play a role in the process of entering the virus into host cells such as ACE2, S glycoprotein, and TMPRSS2, while some target main proteases such as RNA dependent Polymerase and N proteins. On the other hand, one of the causes for the worsening of COVID-19 cases is hyperinflammation. This condition can also be caused by an increase in calcium consumption activity which is responsible for the process of viral endocytosis, mast cell recruitment, and also the recruitment of surrounding cells to form syncytia. Under these conditions, virions that are trapped and accumulated in the syncytia can initiate the release of virions and pro-inflammatory molecules, leading to hyperinflammation and second week crash. This review will explain the importance of the role of calcium ions and mast cells in mediating inflammation as well as the prospect of inhibiting hyperinflammation in COVID19 using flavonoid compounds contained in daily food ingredients
MAKNA TEOLOGI DALAM NOVEL TUHAN MAHA ASYIK SUDJIWO TEDJO DAN DR. MN. KAMBA
Get PDFAbstractGod, with its various mysteries, has given man the element that the search for Him for a search which - apart from being a continuous and unending activity - also gives pleasure. That is why God is most fun: either as an object of study, or as the goal of the entire axis of human activity. The author\u27s understanding of God is very interesting to know, because this book of God is Most Fun is very different from other Theology books. The thinking method used is hermeneutics. The results of research on the novel Tuhan Maha Asik written by Sudjiwo Tedjo and DR. M N. Kamba, shows a direction in unique stories, starting from the discussion of wayang, marhain, worms, self and so on, which is presented with analogies in the style of children. The theological meaning contained in this book is theology of authentic self. Where we can know God through each human being, the self that is meant here is a natural self, not a self that has been entered by concepts formed from society or from outside
Characterization of Adenocarcinoma’s Autofluorescence Properties Using Multiexcitation Analysis Method
No full textGeneral purpose of this research is to get an early cancer detection method based on the properties of optical analysis between normal and adenocarsinoma tissue using the multiexcitation autofluorescence method. Observation of autofluorescence properties was done on the biopsy sample of adenocarcinoma tissues, GR mice transplanted by adenocarsinoma, and cell culture SM 1. Excitation on tissue was done by using the lamp Light Emitting Diode (LED) at some visible light wavelength range. This research obtained that the value of Intensity Auto fluorescence (IAF) at range red wavelength of cells and adenocarsinoma tissues tend to lower compared to the cells normal tissues if its were excited by blue LED. On the contrary, the value of IAF at infra red wavelength from cells and carcinoma tissues tend to higher compared to the cells and normal tissues if its were excited by red LED
In silico Prediction of Sodium-Glucose Co-Transporter-2 (SGLT2) Inhibition Activity by Allium Fistulosum Compound Based on SkelSpheres Molecular Descriptor
Get PDFThe Sodium-Glucose co-transporter-2 (SGLT2) inhibitor represents a novel agent for the treatment of type 2 diabetes. Drugs of this class function by inhibiting glucose reabsorption in the kidneys, thereby controlling blood glucose levels. It is known that SGLT2 inhibitors activate the AMPK signaling pathway by increasing the expression and activity of AMP-activated protein kinase (AMPK). In vivo tests have demonstrated that ethanolic and aqueous extracts of Welsh onion leaves (Allium fistulosum L) can reduce body weight, liver weight, adipocyte size, and enhance AMP-activated protein kinase (AMPK) expression. In this study, the inhibitory activity (IC50) of compounds within Allium fistulosum against SGLT2 was predicted using the Support Vector Regression (SVR) predictive model and the SkelSpheres descriptor. The results of the predicted IC50 measurements for compounds present in the 70% ethanol extract of Allium fistulosum in silico indicate that 4 tyramine derivatives and 1 decursidate compound exhibit Excellent or Potent inhibitor activity criteria (IC50 \u3c 1 µM). Among these, the four tyramine group compounds are the isomers N-trans-feruloyltyramine and N-cis-feruloyltyramine, as well as the isomers N-trans-feruloyl-3\u27-methoxytyramine and N-cis-feruloyl-3\u27-methoxytyramine. The findings of this study suggest that the ability of Allium fistulosum to enhance AMPK expression is possibly achieved through the inhibition of SGLT2
GENE EXPRESSION PROFILE IN SALMONELLA ENTERITIDIS IN EGG ALBUMEN BASED ON PATHOGENICITY VIA GEO-ANALYSIS
Get PDFSalmonella enteriditis, a bacterium known for contaminating egg albumen, serves as a significant causative agent of foodborne illnesses in humans. These illnesses manifest with a spectrum of symptoms ranging from mild to severe, contingent upon the varying pathogenicity levels of Salmonella enteritidis. The central objective of this research endeavor was to meticulously analyze the gene expression profile of Salmonella enteritidis in egg albumin, correlating it with the pathogen's varying pathogenicity levels. This analysis was conducted utilizing the Gene Expression Omnibus (GEO) Analysis framework.A comprehensive examination of 18 genomic databases specific to Salmonella enteritidis, extracted from the GEO Dataset (GSE33102), was undertaken. These databases were methodically clustered in accordance with the pathogenicity gradations of the bacteria. Subsequently, an in-depth analysis and visualization of the data were performed using GEO2R. The analytical findings revealed a notable variance in gene expression, with 35-46 genes demonstrating significant differences (Padj<0.05) when comparing groups with High Pathogenicity and High-Medium Pathogenicity against those with Low PathogenicityThe study culminated in the identification of six distinct gene expressions that effectively discriminate between Salmonella enteritidis groups classified as High, High-Medium, and Low Pathogenicity. This discovery propels the hypothesis that these genes could potentially serve as specific markers for the presence of Salmonella enteritidis in contaminated eggs. Such markers would be instrumental in the early detection of foodborne diseases. However, it is imperative to conduct further research to ascertain the viability of these candidate genes as reliable indicators for the early detection of this pathogen in contaminated food sources
Photo electrochemical detection of dengue-related oligonucleotide sequance anthocyanin as an intercalating agent and electrochromic material
No full textChemoprevention Activity of Temu Mangga Extracts
No full textThe chemoprevention activity of temu mangga extracts was investigated by determination of antioxidant activity with a peroxidation number method and gluthatione-S-transferase (GST) activity in Chang medium culture and cell lysate (total GST activity). The results indicated that ethanol extract has a strong antioxidant activity. It is caused by the phenolic group in the ethanol extract. Treatment Chang cell culture with 7th and 4th ethanol fractions increased the GST activity when compared to the control. The total GST activity (cytosolic and microsomal) increased when Chang cell culture was treated with H2O2/Fe+2. The decrease of the total GST activity was observed when 7th and 4th ethanol fractions were supplemented with H2O2/Fe+2 compared to the cell culture receiving H2O2/Fe+2 only
Photoelectrochemical Detection of Dengue-Related Oligonucleotide Sequence using Anthocyanin as an Intercalating Agent and Electrochromic Material
No full textWorld Health Organization (WHO) presupposes a confirmation of dengue virus infection diagnosis with two criteria, i.e. clinical and laboratory criteria. One of the basic methods used by most laboratories to diagnose dengue virus is to detect oligonucleotide sequence using a DNA amplification technique. In this research, the measurement of denguerelated oligonucleotide was conducted by photoelectrochemical method. The presence of oligonucleotide sequence in target DNA can be detected by DNA probe that is immobilized on TiO2 electrode. The DNA hybrid is then bound to electrochromic substance like anthocyanin that generates current when it is subjected to light. The photocurrent is directly proportional to the number of target DNA. The aim of this research is to obtain photoelectrochemical system that has sensitivity and high responsiveness toward the change in oligonucleotide concentration, especially the applicability of anthocyanin as a electrochromic substance and intercalating agent. Linearity (R2) generated from the change of current in response to concentration changes of target DNA (in the concentration range of 0.75 - 3.00 nM) is 0.9611. Thus, this method has the potential to be developed to detect the presence of dengue virus in biological sample
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