18 research outputs found
KUALITAS SPERMATOZOA CAUDA EPIDIDIMIS SAPI BALI PADA KELOMPOK UMUR YANG BERBEDA
No full textUtilization of cauda epididymis spermatozoa has been carried out because these spermatozoa can be used in artificial insemination or in vitro fertilization. The purpose of this study was to obtain information on the quality of spermatozoa which produced from the cauda epididymis of Bali cattle at different ages. The cauda epididymis samples used came from 31 slaughterhouses. Information and identification of the age of the cattle was based on the structure of the incisors. Observations were made on the variables of motility, concentration, viability and abnormalities of spermatozoa. Each variable was observed in duplicate from each cauda. The data was performed using a t-test to obtain differences in each group. The results showed that the motility was 64.1% in 2 years to 71.2% in 3 years. Spermatozoa concentration were increase from 310.1x107/mL to 401.1x107/mL in 3 years. Viability values were 93.8% to 95.9% and total abnormality was 16.2 to 20.6% in 3 years. The results of statistical analysis showed that there was a significant difference (P0.05) for the concentration, viability and head abnormality of the spermatozoa. Based on the results of the study it can be concluded that the quality of spermatozoa cauda epididymis is affected by the age of the Bali cattle. The concentration, viability and head abnormality values were increased with increasing age of Bali cattle
EFEK PEMBERIAN EKSTRAK KAYU AKWAY (Drymis Sp) TERHADAP KUALITAS SPERMATOZOA MENCIT (Mus musculus L)
No full textThis research was aimed to study the effect of Akway wood (Drymis Sp) extract treatment on spermatozoa quality of mice (Mus musculus L). The extract was given orally once a day in 2 weeks. The animals were divided into three groups; one control group (K1) and two treatment groups with four replicates. K2 group was given 1.3 ml/gr body weight / day and K3 group was given 3.3 ml/ gr body weight / day. The results indicated that treatments were increased significantly (P<0.05) on acrosome integrity and viability, while on the motility, concentration and abnormality treatments had no significant effect
Karakteristik Reproduksi Bandikut Echymipera kalubu) Jantan.
No full textBandicoot (Echymipera kalubu) is one of endemic species in Papua, which has various benefits for the people in Papua. The purpose of this research was to study male reproductive organs characteristics, the quality of cauda epididymal spermatozoa and spermatogenesis processes. This study used 21 males E. kalubu of three different stages of age. Sperm morphology was assessed by using Carbofuchsin (William’s stain) and the stages of spermatogenesis was analized by using Periodic Acid Schiff (PAS) stain. The results showed that reproductive organs comprised of gonad (testis), accessory glands and penis. Testis were elipsoid in shape, the accessory glands consisted of prostat gland and Cowper gland, and the penis was bhipid. Epididymal sperm concentration and motility increased with sperm maturity. The length of sperm head, midpiece and principal piece were 2.91±0.40 μm, 13.99±0.87 μm and 145.59±5.38 μm, respectively and the total length of spermatozoa was 162.51±5.12 μm. Finally, E. kalubu had nine stages of spermatogenesis with ten step development of spermatids into spermatozoa.Bandikut (Echymipera kalubu) merupakan salah satu satwa endemik Papua yang saat ini statusnya masih sebagai hewan liar dan populasinya masih berlimpah pada habitat yang sesuai. Echymipera kalubu memiliki beberapa keunggulan, antara lain: memiliki laju reproduksi yang paling tinggi diantara semua marsupialia dengan jumlah anak perkelahiran 2-4 ekor, merupakan hewan marsupialia (berkantung) yang dagingnya dimanfaatkan sebagai sumber protein hewani serta memiliki nilai etno-zoologis (rambut, tulang dan anak bandikut umur 12 hari dipercaya berkhasiat untuk pengobatan) bagi masyarakat di Papua dan mempunyai struktur organ reproduksi yang unik, dimana saluran akhir alat reproduksi, saluran kencing dan pembuangan feces bermuara dalam satu saluran anus mirip kloaka pada unggas. Informasi mengenai status biologi reproduksi E. kalubu yang hidup liar di alam hingga saat ini belum banyak dilaporkan. Hal ini menyebabkan perlu dilakukan penelitian mengenai karakteristik dari organ reproduksi jantan, yang terdiri dari: morfologi organ kelamin, kualitas spermatozoa yang berasal dari epididimis serta gambaran spermatogenesis pada E. kalubu sebagai data dasar yang akan mendukung perkembangbiakan di penangkaran. Bandikut (E. kalubu) jantan yang digunakan sebanyak 21 ekor berasal dari kabupaten Manokwari. Penelitian terbagi menjadi tiga tahap, yaitu pengamatan karakteristik organ reproduksi, karakteristik spermatozoa asal epidimis (motilitas, konsentrasi dan morfologi spermatozoa) dan tahapan spermatogenesis. Data-data yang diperoleh kemudian dianalisis secara deskriptif dan variabel yang diukur adalah rata-rata yang didapat dari setiap variabel dengan menggunakan standar deviasi
Pengaruh Ekstrak Sarang Semut (Myrmecodia Sp) dalam Pengencer Ringer Laktat Terhadap Abnormalitas dan Viabilitas Spermatozoa Ayam Kampung
No full textAntioxidants can inhibit the oxidation process by reacting with free radicals, which in turn, maintain the quality of spermatozoa. This study aims to determine the viability and the abnormality of “ayam kampung” (Gallus gallus) spermatozoa after being diluted in ringer lactate infused with “sarang semut” (Myrmecodia Sp) extract. RAL is the research method used, utilizing 4 different Gallus gallus as test subjects. The variables observed are the abnormality and the viability of the spermatozoa sample treated with different amounts of Myrmecodia Sp extract, P0: 0 ml, P1: 0.02 ml, P2: 0.03, and P3: 0.04 ml. The abnormality rate for each sample is as follows; P0: 14 ± 4.05%, P1: 13.5 ± 3.78%, P2: 3.5 ± 1.23% and P3: 12.83 ± 5.04 %. Using the variance analysis, the result showed that the addition of Myrmecodia Sp extract in ringer lactate solution had little to no effect on causing spermatozoa abnormalities. Furthermore, the viability of spermatozoa for each sample is; P0: 14 ± 4.05%, P1: 13 ± 3.78%, P2: 13 ± 1.22% and P3: 12 ± 5.04%. Based on the variance analysis, the result showed that Myrmecodia Sp extract had a significant effect (P <0.05) on the spermatozoa viability. The addition of Myrmecodia sp extract to ringer lactate diluter had little to no effect on the abnormality of the Gallus gallus’ spermatozoa but had a significant effect on the spermatozoa’s viability
Analisis cemaran mikrobiologis pangan asal ternak pada jajanan anak sekolah dasar: Microbiological analysis contamination of children’s snacks elementary school
No full textAbstract
This study examined the microbiological contamination of street food that consumed by children at primary. The study, specifically focused on the detection of total microorganisms such as Escherichia coli, and Salmonella sp. Microbiological analyses is one way to determine the level of hygiene of snack foods. Presence of microbes determined by using Total Plate Count (TPC), detection test was applied to detect E.coli and Salmonella sp test applied detect present of Salmonella sp. Quantitatif and qualitatif methods have been sed with sampel snacks collection were obtained street food at primary schools. TPC test results showed that 6 samples of snacks have been contaminated with microbes with the threshold of the maximum limit. Those snacks, namely G1 (batagor) 2.6 x 10-5 cfu/gr, K1 (batagor) 1.3 x 10-5 cfu/gr, L2(egg meat ball) 2.3 x 10-5 cfu/gr, M1 (egg roll) 3.8 x 10-5 cfu/gr, N (meatball) 1.1 x 10-5 cfu/gr and Q1 (batagor) 1.1 x 10-5 cfu/gr. Results Salmonella sp test indicated that there were 9 samples positively contaminated with salmonella sp bacteria. Those are D2 (chicken sausage), E1 (egg roll), E2 (chicken meat ball), F2 (chicken meat ball), G1 (batagor), G2 (fried sausage), N (meatball), P1 (nuggets), P2 (chicken meat ball). Escherchia coli detection test results all samples were declared negative. There were 6 school locations (G, K, L, M, N, Q) different snack samples contaminated with microbes, 6 school locations (D, E, F, G ,N, P) snack samples were contaminated with Salmonella sp. Those snacks were declared not recommended for consumption.
Keywords: Contamination; E. coli; Microbes; Salmonella sp; Snacks
Abstrak
Penelitian ini meneliti kontaminasi mikrobiologi pada jajanan yang ditujukan untuk anak-anak sekolah dasar di distrik Manokwari Barat, dengan fokus khusus pada deteksi mikroorganisme total, E. coli, dan Salmonella sp. Salah satu cara untuk mengetahui tingkat higienitas dari pangan jajanan adalah dengan analisis mikrobiologi berupa uji keberadaan mikroba dengan Total Plate Count (TPC), uji deteksi E.coli dan uji Salmonella sp. Sampel jajanan hasil olahan ternak diperoleh dari jajanan yang ada di Sekolah Dasar di wilayah distrik Manokwari Barat. Penelitian ini menggunakan gabungan metode kualitatif dan kuantitatif dengan menggunakan teknik pengambilan sampel yaitu purposive sampling. Hasil uji TPC pada jajanan pangan hasil ternak di Sekolah Dasar distrik Manokwari Barat terdapat 6 sampel yang berada diambang batas maksimum yaitu sampel G1 (batagor) 2.6 x 10-5 cfu/gr, K1 (batagor) 1.3 x 10-5cfu/gr L2 (pentol telur) 2.3 x 10-5 cfu/gr. M1 (telur gulung) 3.8 x 10-5 cfu/gr, N (pentol bakso) 1.1 x 10-5 cfu/gr dan Q1 (batagor) 1.1 x 10-5 cfu/gr. Hasil pengujian deteksi Salmonella sp terdapat 9 sampel yang positif tercemar bakteri Salmonella sp. D2 (sosis ayam), E1 (telur gulung), E2 (pentol ayam), F2 (pentol ayam), G1 (batagor), G2 (sosis goreng), N (pentol bakso), P1 (nugget), P2 (pentol ayam) sedangkan untuk hasil uji deteksi E. coli semua sampel dinyatakan negatif. Dari hasil penelitian ini dinyatakan bahwa ada 6 lokasi (G, K, L, M, N, Q) telah terkontaminasi mikroba diambang batas dan tidak layak untuk dikonsumsi dan 6 lokasi (D, E, F, G, N, P) telah terkontaminasi Salmonella sp dan dinyatakan tidak layak untuk dikonsumsi.
Kata kunci: E. coli; Mikroba; Pangan jajanan; Salmonella sp; Total plate coun
EFEK ANTIKOLESTEROL FRAKSI n-HEKSANA RUMPUT KEBAR PADA HEWAN MODEL HIPERLIPIDAEMIA
No full textPenelitian ini bertujuan mengetahui efek antikolesterol fraksi n-heksana ekstrak rumput kebar pada hewan model hiperlipidaemia. Delapan kelinci dewasa dibagi dalam dua kelompok. Kelompok kontrol (K1) hanya diberikan diet tinggi lemak selama 1 bulan dan pada kelompok perlakuan (K2) diberikan diet tinggi lemak ditambah fraksi n-heksana ekstrak rumput kebar. Uji keberhasilan penelitian dilakukan dengan pemeriksaan kolesterol total serum darah. Hasil penelitian menunjukkan konsentrasi kolesterol total serum darah pada K1 dan K2 masing-masing adalah 100,00±18,28 dan 47,75±12,53 mg/dl. Dari hasil penelitian disimpulkan bahwa fraksi n-heksanaa mampu menurunkan tingkat kolesterol total dalam serum darah
Konsentrasi dan Motilitas Spermatozoa Ayam Kampung (Gallus domesticus) dalam Pengencer Ringer Laktat Yang Diberi Tambahan Minyak Buah Merah (Pandanus conoideus Lam)
No full textRed fruit oil is a red fruit extraction product (Pandanus conoideus Lam) containing a lot of antioxidants such as carotenoids, tocopherols as well as unsaturated fatty acids such as oleic acid, linoleic acid, linolenic acid and decanoic acid which is potential to be used as an additive for semen chicken diluents. This study aims to obtain an information about the effectiveness of adding red fruit oil as a cement diluent derived from male native chickens. The treatment consisted of 0.9 ml ringer laktat + 0.1 ml semen (Pl), ringer laktat 0.98 ml + 0.1 ml semen + 0.02 ml red fruit oil (P2), ringer laktat 0.96 ml + 0.1 ml semen + 0.04 ml red fruit oil (P3), ringer laktat 0.94 ml + 0.1 ml cement + 0.06 ml red fruit oil (P4). The results showed the sperm motility ranged from 48.17 ± 7.86% to 84.67 ± 2.42%. Treatment P1 and P2 have a very significant difference (P 0.05), nor did among treatments P2, P3 and P4. However, treatment P1 has a significant difference on spermatozoa concentration with P3 and P4. (P <0.05). It can be concluded that the addition of red fruit oil in lactated ringer diluent can be used as an additive for semen diluents because it is able to maintain the concentration. In addition, adding of red fruit oil up to concentrations of more than 2% tend to reduce sperm motility.
Keywords: Concentration, Motility, Spermatozoa and Red fruit oi
KARAKTERISTIK HEMATOLOGI BURUNG GOSONG KELAM (Megapodius freycinet) DI PULAU MANSINAM MANOKWARI: HAEMATOLOGY CHARACTERISTIC OF GOSONG KELAM BIRD (Megapodius freycinet) IN MANSINAM ISLAND MANOKWARI
No full textDecreasing population of Gosong Kelam birds (Megapodius freycinet) can be limited by using captivity. Characteristic of haematology were indicator to know about health and physiology satus of animals in a captivity. The aim of the research was to know haematology information of Gosong Kelam birds (Megapodius freycinet) in Mansinam island. The results show that average haematologies value of Gosong Kelam birds were not different to other research results. The number of erythrocyte 2.6x103/mm3, hemoglobin 15.8 gr%, leukocyte 15.9x103/mm3. More over types of leukocyte which are consist of basophils with 3.25% without eosinophils, heterophils 23.5%, lymphocytes 61.75% and monocytes 13%
Karakteristik dan Jenis Pengencer Semen Sapi Bali: Suatu Review: Bali Cattle Semen Characteristics and Diluent Types: A Review
No full textAbstract
Bali cattle are Indonesian native cattle that are preferred because they have adaptability and reproductive efficiency capabilities. The productivity of this cow can be increased using artificial insemination. The success of IB implementation is determined by the quality of the semen used. This article aims to provide an overview of the quality of fresh semen, after dilution and the types of diluents that have generally been used to maintain Bali cattle spermatozoa. The quality of fresh semen provides an overview of the condition of the semen after storage. Types of diluents that have been used are chemical diluents such as tris aminomethane, CEP, skim milk, andromed and natural diluents such as coconut water, carrot juice, guava filtrate, honey and tomato juice. Bali cattle semen stored in each diluent gives a different quality response depending on the diluent used. This diluent has a different response to semen qualities in cold and frozen storage.
Keywords: Quality of semen; Diluent; Bali cattle; Spermatozoa
Abstrak
Sapi Bali merupakan sapi asli Indonesia yang disukai karena memiliki kemampuan adaptasi dan kemampuan efisiensi reproduksi. Produktivitas sapi ini dapat ditingkatkan menggunakan inseminasi buatan. Keberhasilan pelaksanaan IB ditentukan oleh kualitas semen yang digunakan. Artikel ini bertujuan untuk memberikan gambaran tentang kualitas semen segar, setelah pengenceran dan jenis pengencer yang umumnya telah digunakan untuk mempertahankan spermatozoa sapi Bali. Kualitas semen segar memberikan gambaran kondisi semen setelah penampungan. Jenis pengencer yang telah digunakan adalah pengencer dari bahan kimia seperti tris aminomethane, CEP, susu skim, andromed dan pengencer alternatif seperti air kelapa, sari wortel, filtrat jambu biji, madu dan sari buah tomat. Semen sapi Bali yang disimpan pada masing-masing pengencer memberikan respon kualitas yang berbeda-beda tergantung bahan pengencer yang digunakan. Secara umum pengencer yang digunakan mampu mempertahankan kualitas semen sapi Bali.
Kata kunci: Kualitas semen; Pengencer; Sapi Bali; Spermatozo
Pengaruh Sari Buah Merah (Pandanus conoideus Lam) terhadap Abnormalitas Spermatozoa Ayam Kampung
No full textAntioxidants are substances that can prevent the occurrence of free radical antioxidation reactions in lipid oxidation. Red fruit (Pandanus conoideus Lam) is one of the plants endemic to Papua which contain high antioxidant. The aim of this research was to determine the abnormality spermatozoa of ayam Kampung which were received red fruit extract treatment. Eight cokcs were divided into 4 treatments, each treatment was repeated 5 times. The treatment consisted of control (P0) without extract, P1 with 0.5 mL extract/kgBW/day, P2 with 1 mL extract/kgBW/day and P3 with 1.5 mL extract/kgBW/day. The results showed that red fruit extract decreased the percentage of abnormality spermatozoa (P<0.05) compare to control (without red fruit extract). Forms of abnormality obtained was abnormalities on the head, namely swelled head, bent head, head detachement, knotted tail and smaller head, middle abnormalities, namely midpiece detachement and bending at midpiece border and abnormalities of the tail, namely bent tail, knotted tail, tail detachement and curled tail
