11 research outputs found
KUALITAS SPERMATOZOA CAUDA EPIDIDIMIS SAPI BALI PADA KELOMPOK UMUR YANG BERBEDA
No full textUtilization of cauda epididymis spermatozoa has been carried out because these spermatozoa can be used in artificial insemination or in vitro fertilization. The purpose of this study was to obtain information on the quality of spermatozoa which produced from the cauda epididymis of Bali cattle at different ages. The cauda epididymis samples used came from 31 slaughterhouses. Information and identification of the age of the cattle was based on the structure of the incisors. Observations were made on the variables of motility, concentration, viability and abnormalities of spermatozoa. Each variable was observed in duplicate from each cauda. The data was performed using a t-test to obtain differences in each group. The results showed that the motility was 64.1% in 2 years to 71.2% in 3 years. Spermatozoa concentration were increase from 310.1x107/mL to 401.1x107/mL in 3 years. Viability values were 93.8% to 95.9% and total abnormality was 16.2 to 20.6% in 3 years. The results of statistical analysis showed that there was a significant difference (P0.05) for the concentration, viability and head abnormality of the spermatozoa. Based on the results of the study it can be concluded that the quality of spermatozoa cauda epididymis is affected by the age of the Bali cattle. The concentration, viability and head abnormality values were increased with increasing age of Bali cattle
Karakteristik Reproduksi Bandikut Echymipera kalubu) Jantan.
No full textBandicoot (Echymipera kalubu) is one of endemic species in Papua, which has various benefits for the people in Papua. The purpose of this research was to study male reproductive organs characteristics, the quality of cauda epididymal spermatozoa and spermatogenesis processes. This study used 21 males E. kalubu of three different stages of age. Sperm morphology was assessed by using Carbofuchsin (William’s stain) and the stages of spermatogenesis was analized by using Periodic Acid Schiff (PAS) stain. The results showed that reproductive organs comprised of gonad (testis), accessory glands and penis. Testis were elipsoid in shape, the accessory glands consisted of prostat gland and Cowper gland, and the penis was bhipid. Epididymal sperm concentration and motility increased with sperm maturity. The length of sperm head, midpiece and principal piece were 2.91±0.40 μm, 13.99±0.87 μm and 145.59±5.38 μm, respectively and the total length of spermatozoa was 162.51±5.12 μm. Finally, E. kalubu had nine stages of spermatogenesis with ten step development of spermatids into spermatozoa.Bandikut (Echymipera kalubu) merupakan salah satu satwa endemik Papua yang saat ini statusnya masih sebagai hewan liar dan populasinya masih berlimpah pada habitat yang sesuai. Echymipera kalubu memiliki beberapa keunggulan, antara lain: memiliki laju reproduksi yang paling tinggi diantara semua marsupialia dengan jumlah anak perkelahiran 2-4 ekor, merupakan hewan marsupialia (berkantung) yang dagingnya dimanfaatkan sebagai sumber protein hewani serta memiliki nilai etno-zoologis (rambut, tulang dan anak bandikut umur 12 hari dipercaya berkhasiat untuk pengobatan) bagi masyarakat di Papua dan mempunyai struktur organ reproduksi yang unik, dimana saluran akhir alat reproduksi, saluran kencing dan pembuangan feces bermuara dalam satu saluran anus mirip kloaka pada unggas. Informasi mengenai status biologi reproduksi E. kalubu yang hidup liar di alam hingga saat ini belum banyak dilaporkan. Hal ini menyebabkan perlu dilakukan penelitian mengenai karakteristik dari organ reproduksi jantan, yang terdiri dari: morfologi organ kelamin, kualitas spermatozoa yang berasal dari epididimis serta gambaran spermatogenesis pada E. kalubu sebagai data dasar yang akan mendukung perkembangbiakan di penangkaran. Bandikut (E. kalubu) jantan yang digunakan sebanyak 21 ekor berasal dari kabupaten Manokwari. Penelitian terbagi menjadi tiga tahap, yaitu pengamatan karakteristik organ reproduksi, karakteristik spermatozoa asal epidimis (motilitas, konsentrasi dan morfologi spermatozoa) dan tahapan spermatogenesis. Data-data yang diperoleh kemudian dianalisis secara deskriptif dan variabel yang diukur adalah rata-rata yang didapat dari setiap variabel dengan menggunakan standar deviasi
Pengaruh Ekstrak Sarang Semut (Myrmecodia Sp) dalam Pengencer Ringer Laktat Terhadap Abnormalitas dan Viabilitas Spermatozoa Ayam Kampung
No full textAntioxidants can inhibit the oxidation process by reacting with free radicals, which in turn, maintain the quality of spermatozoa. This study aims to determine the viability and the abnormality of “ayam kampung” (Gallus gallus) spermatozoa after being diluted in ringer lactate infused with “sarang semut” (Myrmecodia Sp) extract. RAL is the research method used, utilizing 4 different Gallus gallus as test subjects. The variables observed are the abnormality and the viability of the spermatozoa sample treated with different amounts of Myrmecodia Sp extract, P0: 0 ml, P1: 0.02 ml, P2: 0.03, and P3: 0.04 ml. The abnormality rate for each sample is as follows; P0: 14 ± 4.05%, P1: 13.5 ± 3.78%, P2: 3.5 ± 1.23% and P3: 12.83 ± 5.04 %. Using the variance analysis, the result showed that the addition of Myrmecodia Sp extract in ringer lactate solution had little to no effect on causing spermatozoa abnormalities. Furthermore, the viability of spermatozoa for each sample is; P0: 14 ± 4.05%, P1: 13 ± 3.78%, P2: 13 ± 1.22% and P3: 12 ± 5.04%. Based on the variance analysis, the result showed that Myrmecodia Sp extract had a significant effect (P <0.05) on the spermatozoa viability. The addition of Myrmecodia sp extract to ringer lactate diluter had little to no effect on the abnormality of the Gallus gallus’ spermatozoa but had a significant effect on the spermatozoa’s viability
Analisis cemaran mikrobiologis pangan asal ternak pada jajanan anak sekolah dasar: Microbiological analysis contamination of children’s snacks elementary school
No full textAbstract
This study examined the microbiological contamination of street food that consumed by children at primary. The study, specifically focused on the detection of total microorganisms such as Escherichia coli, and Salmonella sp. Microbiological analyses is one way to determine the level of hygiene of snack foods. Presence of microbes determined by using Total Plate Count (TPC), detection test was applied to detect E.coli and Salmonella sp test applied detect present of Salmonella sp. Quantitatif and qualitatif methods have been sed with sampel snacks collection were obtained street food at primary schools. TPC test results showed that 6 samples of snacks have been contaminated with microbes with the threshold of the maximum limit. Those snacks, namely G1 (batagor) 2.6 x 10-5 cfu/gr, K1 (batagor) 1.3 x 10-5 cfu/gr, L2(egg meat ball) 2.3 x 10-5 cfu/gr, M1 (egg roll) 3.8 x 10-5 cfu/gr, N (meatball) 1.1 x 10-5 cfu/gr and Q1 (batagor) 1.1 x 10-5 cfu/gr. Results Salmonella sp test indicated that there were 9 samples positively contaminated with salmonella sp bacteria. Those are D2 (chicken sausage), E1 (egg roll), E2 (chicken meat ball), F2 (chicken meat ball), G1 (batagor), G2 (fried sausage), N (meatball), P1 (nuggets), P2 (chicken meat ball). Escherchia coli detection test results all samples were declared negative. There were 6 school locations (G, K, L, M, N, Q) different snack samples contaminated with microbes, 6 school locations (D, E, F, G ,N, P) snack samples were contaminated with Salmonella sp. Those snacks were declared not recommended for consumption.
Keywords: Contamination; E. coli; Microbes; Salmonella sp; Snacks
Abstrak
Penelitian ini meneliti kontaminasi mikrobiologi pada jajanan yang ditujukan untuk anak-anak sekolah dasar di distrik Manokwari Barat, dengan fokus khusus pada deteksi mikroorganisme total, E. coli, dan Salmonella sp. Salah satu cara untuk mengetahui tingkat higienitas dari pangan jajanan adalah dengan analisis mikrobiologi berupa uji keberadaan mikroba dengan Total Plate Count (TPC), uji deteksi E.coli dan uji Salmonella sp. Sampel jajanan hasil olahan ternak diperoleh dari jajanan yang ada di Sekolah Dasar di wilayah distrik Manokwari Barat. Penelitian ini menggunakan gabungan metode kualitatif dan kuantitatif dengan menggunakan teknik pengambilan sampel yaitu purposive sampling. Hasil uji TPC pada jajanan pangan hasil ternak di Sekolah Dasar distrik Manokwari Barat terdapat 6 sampel yang berada diambang batas maksimum yaitu sampel G1 (batagor) 2.6 x 10-5 cfu/gr, K1 (batagor) 1.3 x 10-5cfu/gr L2 (pentol telur) 2.3 x 10-5 cfu/gr. M1 (telur gulung) 3.8 x 10-5 cfu/gr, N (pentol bakso) 1.1 x 10-5 cfu/gr dan Q1 (batagor) 1.1 x 10-5 cfu/gr. Hasil pengujian deteksi Salmonella sp terdapat 9 sampel yang positif tercemar bakteri Salmonella sp. D2 (sosis ayam), E1 (telur gulung), E2 (pentol ayam), F2 (pentol ayam), G1 (batagor), G2 (sosis goreng), N (pentol bakso), P1 (nugget), P2 (pentol ayam) sedangkan untuk hasil uji deteksi E. coli semua sampel dinyatakan negatif. Dari hasil penelitian ini dinyatakan bahwa ada 6 lokasi (G, K, L, M, N, Q) telah terkontaminasi mikroba diambang batas dan tidak layak untuk dikonsumsi dan 6 lokasi (D, E, F, G, N, P) telah terkontaminasi Salmonella sp dan dinyatakan tidak layak untuk dikonsumsi.
Kata kunci: E. coli; Mikroba; Pangan jajanan; Salmonella sp; Total plate coun
Konsentrasi dan Motilitas Spermatozoa Ayam Kampung (Gallus domesticus) dalam Pengencer Ringer Laktat Yang Diberi Tambahan Minyak Buah Merah (Pandanus conoideus Lam)
No full textRed fruit oil is a red fruit extraction product (Pandanus conoideus Lam) containing a lot of antioxidants such as carotenoids, tocopherols as well as unsaturated fatty acids such as oleic acid, linoleic acid, linolenic acid and decanoic acid which is potential to be used as an additive for semen chicken diluents. This study aims to obtain an information about the effectiveness of adding red fruit oil as a cement diluent derived from male native chickens. The treatment consisted of 0.9 ml ringer laktat + 0.1 ml semen (Pl), ringer laktat 0.98 ml + 0.1 ml semen + 0.02 ml red fruit oil (P2), ringer laktat 0.96 ml + 0.1 ml semen + 0.04 ml red fruit oil (P3), ringer laktat 0.94 ml + 0.1 ml cement + 0.06 ml red fruit oil (P4). The results showed the sperm motility ranged from 48.17 ± 7.86% to 84.67 ± 2.42%. Treatment P1 and P2 have a very significant difference (P 0.05), nor did among treatments P2, P3 and P4. However, treatment P1 has a significant difference on spermatozoa concentration with P3 and P4. (P <0.05). It can be concluded that the addition of red fruit oil in lactated ringer diluent can be used as an additive for semen diluents because it is able to maintain the concentration. In addition, adding of red fruit oil up to concentrations of more than 2% tend to reduce sperm motility.
Keywords: Concentration, Motility, Spermatozoa and Red fruit oi
Pengaruh Sari Buah Merah (Pandanus conoideus Lam) terhadap Abnormalitas Spermatozoa Ayam Kampung
No full textAntioxidants are substances that can prevent the occurrence of free radical antioxidation reactions in lipid oxidation. Red fruit (Pandanus conoideus Lam) is one of the plants endemic to Papua which contain high antioxidant. The aim of this research was to determine the abnormality spermatozoa of ayam Kampung which were received red fruit extract treatment. Eight cokcs were divided into 4 treatments, each treatment was repeated 5 times. The treatment consisted of control (P0) without extract, P1 with 0.5 mL extract/kgBW/day, P2 with 1 mL extract/kgBW/day and P3 with 1.5 mL extract/kgBW/day. The results showed that red fruit extract decreased the percentage of abnormality spermatozoa (P<0.05) compare to control (without red fruit extract). Forms of abnormality obtained was abnormalities on the head, namely swelled head, bent head, head detachement, knotted tail and smaller head, middle abnormalities, namely midpiece detachement and bending at midpiece border and abnormalities of the tail, namely bent tail, knotted tail, tail detachement and curled tail
Karakteristik dan Jenis Pengencer Semen Sapi Bali: Suatu Review: Bali Cattle Semen Characteristics and Diluent Types: A Review
No full textAbstract
Bali cattle are Indonesian native cattle that are preferred because they have adaptability and reproductive efficiency capabilities. The productivity of this cow can be increased using artificial insemination. The success of IB implementation is determined by the quality of the semen used. This article aims to provide an overview of the quality of fresh semen, after dilution and the types of diluents that have generally been used to maintain Bali cattle spermatozoa. The quality of fresh semen provides an overview of the condition of the semen after storage. Types of diluents that have been used are chemical diluents such as tris aminomethane, CEP, skim milk, andromed and natural diluents such as coconut water, carrot juice, guava filtrate, honey and tomato juice. Bali cattle semen stored in each diluent gives a different quality response depending on the diluent used. This diluent has a different response to semen qualities in cold and frozen storage.
Keywords: Quality of semen; Diluent; Bali cattle; Spermatozoa
Abstrak
Sapi Bali merupakan sapi asli Indonesia yang disukai karena memiliki kemampuan adaptasi dan kemampuan efisiensi reproduksi. Produktivitas sapi ini dapat ditingkatkan menggunakan inseminasi buatan. Keberhasilan pelaksanaan IB ditentukan oleh kualitas semen yang digunakan. Artikel ini bertujuan untuk memberikan gambaran tentang kualitas semen segar, setelah pengenceran dan jenis pengencer yang umumnya telah digunakan untuk mempertahankan spermatozoa sapi Bali. Kualitas semen segar memberikan gambaran kondisi semen setelah penampungan. Jenis pengencer yang telah digunakan adalah pengencer dari bahan kimia seperti tris aminomethane, CEP, susu skim, andromed dan pengencer alternatif seperti air kelapa, sari wortel, filtrat jambu biji, madu dan sari buah tomat. Semen sapi Bali yang disimpan pada masing-masing pengencer memberikan respon kualitas yang berbeda-beda tergantung bahan pengencer yang digunakan. Secara umum pengencer yang digunakan mampu mempertahankan kualitas semen sapi Bali.
Kata kunci: Kualitas semen; Pengencer; Sapi Bali; Spermatozo
KONSENTRASI DAN MOTILITAS SPERMATOZOA CAUDA EPIDIDIMIS BANDIKUT (Echymipera kalubu): SPERM CONCENTRATIONAND MOTILITYOF CAUDA EPIDIDYMIS BANDIKUT (Echymipera kalubu)
No full textOne of the factorthat influencethe process offertilization on Echymiperakalubu is the quality of the spermwhich produced by males. The ability of spermtofertilize anovumis not only owned by the spermatozoa which derived from the results of ejaculation, butis also owned by spermatozoa from the caudaepididymis.This study aimed to determine sperm concentration and motility from caudaepididymis.Twenty-one males E. kalubuwere used which was 370-1500g. Based onthis weight, it was divided into three groupings, they were prepubertal (370-640 g), young adult (>640-850g) and adult (>850g).The results showed that the sperm concentration of the prepubertal was1.75±1.14x106/ml,the young adult was 8.86±4.21x106/ml and increased to12.56±6.33x106/mlin the adult group.Sperm motility also increased with increasing maturity. The prepuberta lgroup showed that sperm motility was33.33±25.17%, increased to41.67±12.58% (young adult) and3.54±57.50% followed by the adult
DISEMINASI TEKNOLOGI PENGOLAHAN BRIKET BERBAHAN DASAR KOTORAN KAMBING DAN LIMBAH SERBUK GERGAJI KAYU DI DISTRIK PRAFI KABUPATEN MANOKWARI
No full textBiomassa yang berpotensi sebagai sumber energi terbaharukan jumlahnya sangat melimpah di Indonesia, biasanya memiliki nilai ekonomi rendah, serta merupakan limbah setelah diambil produk primernya. Limbah peternakan dan olahan kayu merupakan biomassa yang masih menjadi masalah bagi lingkungan, karena rendahnya pengetahuan masyarakat tentang pengolahan limbah. Masyarakat juga mangalami masalah kelangkaan bahan bakar minyak (BBM) dan rendahnya pendapatan, karena hanya bertumpu pada hasil pertanian. Solusi untuk mengatasi masalah tersebut adalah pengolahan limbah menjadi briket berbahan dasar campuran kotoran kambing dan serbuk gergaji kayu. Kegiatan pemberdayaan masyarakat ini bertujuan untuk mendiseminasikan dan mendorong penerapan inovasi teknologi pengolahan limbah tepat guna dan memberikan alternatif potensi usaha yang dapat dikembangkan oleh masyarakat Distrik Prafi Kabupaten Manokwari. Metode diseminasi teknologi yang diterapkan adalah penyuluhan partisipatif, pelatihan, demonstrasi, dan pendampingan masyarakat, hingga menjadi produk yang siap dipasarkan. Pengolahan kotoran kambing dan serbuk gergaji kayu menjadi briket dilakukan melalui tahapan pengeringan bahan baku, penggilingan, pencampuran bahan perekat, pencetakan, pengeringan, pengujian, dan pengemasan. Hasil menunjukkan bahwa kegiatan yang dilaksanakan (1) berhasil mendiseminasikan inovasi teknologi pengolahan limbah peternakan dan olahan kayu menjadi sumber energi sebagai bahan bakar alternatif, (2) masyarakat terlibat secara aktif dalam semua kegiatan, dan (3) briket berbahan dasar kotoran kambing dan serbuk gergaji dapat menjadi sumber energi terbaharukan bagi masyarakat.
Kata kunci: Briket, Kotoran kambing, Serbuk gergaji kayu, Distrik Prafi
ABSTRACT
The potentially biomass as a source of renewable energy is very abundant in Indonesia. It is waste of primer product and has low economic value. Livestock waste and processed wood waste are biomass that become an issue to the environment, due to the low community knowledge on the waste processing. Those wastes can be used as a source of renewable energy to tackle scarcity of fuel oil problem of the community. The using of combination of goat manure and sawdust wood as raw material of briquettes can be as a solution to environment and fuel availability problem. The community development activities aimed to disseminate and promote effective innovations technological waste treatment and provide an alternative business potential that may be developed by the Prafi District community, Manokwari Regency. The method of dissemination of technology is participatory counseling, in-house training, a demonstration, community assistance, and product marketing. The briquettes process consist of drying raw materials, milling, refining, adhesive mixing, printing, briquetes drying, testing of briquette quality, and packaging. The result showed that (1) briquettes technology had been successfully transfer to the community, (2) Participants of the community development activity were enthusiastic and involve that indicated by their participation, (3) The briquettes can be a source of renewable energy for the community.
Keywords: Briquettes, Goat manure, Sawdust wood, Prafi Distric
Sifat Kuantitatif Ayam Joper yang Dipelihara Kelompok Peternak “Papua Mandiri†di Kabupaten Manokwari Propinsi Papua Barat
No full textSifat kuantitatif yang dimiliki oleh ayam Joper seperti ukuran tubuh merupakan salah satu parameter penting dalam prog pemuliaan ternak khususnya untuk mengetahui tingkat pertumbuhannya. Penelitian ini bertujuan untuk mengetahui produktivitas ayam yang telah dipelihara selama dua bulan, menggunakan hubungan antara bobot badan dengan ukuran-ukuran tubuh ayam Joper. Penelitian menggunakan metode deskripsi dengan teknik observasi. Data yang diukur berupa ukuran kuantitatif. Sampel ayam ditentukan secara purposif sampling pada kedua lokasi penelitian sebanyak 66 ekor. Data yang diperoleh dianalisa secara tabulasi dan korelasi regresi dengan menggunakan SPSS versi 25. Bobot badan ayam Joper umur 2 bulan menggambarkan nilai yang cukup baik, karena memenuhi standar bobot badan ayam, yaitu 1015,5 g. Bobot badan ayam jantan adalah 1113,94 g dan betina dalah 916,36 g. Nilai korelasi pearson dan R square tertinggi adalah korelasi dan regresi panjang kaki terhadap bobot badan, sehingga ukuran panjang kaki dapat digunakan untuk menduga bobot badan ayam Joper pada usia 2 bulan.
