502 research outputs found
QUANTITATIVE ANALYSIS OF SERUM BONE ALP (ALP Ⅲ) IN AFFlNITY ELECTROPHORESIS AND THE PRECIPITATION METHOD CONTAINING LECTIN
Get PDFFor decades, ALP had been interpreted as a marker enzyme of metabolic bone diseases and an isoenzyme specific for the osteoblast been widely known as ALP Ⅲ, too. Moreover, current tremendous advances in dialysis technology project an impact for the clinical significance of ALP Ⅲ, because of the advent of a new characteristic clinical entity formed renal osteodystrophy (ROD) which had been one of the inevitable complications in long term hemodialysis patients. So far, however, many conventional methods failed to separate clearly ALP Ⅲ from the liver specific ALP (ALP Ⅱ) and present determination of ALP Ⅲ by no means satisfied the clinical demand due to the lack of a direct quantitative method. On the other hand, lectin method, reported originally in 1984 by Rosalki, proved it to be possible to measure ALP Ⅲ directly as well as quantatively. So, the author performed this study to confirm more precisely several conditions of procedure of the lectin method, using established osteoblastic cell line ROS 17/2 as a marker, as well as the availability of its clinical application, and obtained the following results ; 1) The optimum concentration of Wheat-Germ lectin binded fully to ALP Ⅲ was 139 μmol/ml in distilled water. 2) The concentration of Triton X-100 preventing biliary ALP from ALP-lectin complex was 20%. 3) ALP Ⅲ activity incubated with lectin overnight at 4℃ after incubation for 30 min. at 37℃ was higher (mean±S.D, 24.0±15.2%) than that incubated with lectin for 30 min. at 37℃. 4) ALP Ⅲ was clearly separated from the liver fraction using affinity electrophoresis on polyacrylamide gel disc and isoelectric focusing on agarose gel. 5) ALP Ⅲ activity in precipitate showed good correlation (r=0.998) with the differential between total ALP activity and ALP Ⅱ activity in supernate
Factions in the ALP
No full textThis record was harvested from a previous catalogue system and will be withdrawn in 2025. Information in this record may be superseded or incomplete. Visit this record in UMA's new catalogue at: https://archives.library.unimelb.edu.au/nodes/view/317496Article or speech, unidentified author and audience, mostly likely created in 2001 owing to statements in text about the Labor Party's 100 year anniversary.280917
item: [2010.0053.01109] "Factions in the ALP
Treatment with cucurbitacin B alone and in combination with gefitinib induces cell cycle inhibition and apoptosis via EGFR and JAK/STAT pathway in human colorectal cancer cell lines
No full textBayram, Cem/0000-0001-8717-4668; Elmazoglu, Zubeyir/0000-0003-4527-8834WOS: 000375574200007PubMed: 26183715The epidermal growth factor receptor (EGFR) associated with signaling pathways, such as Janus kinase (JAK)/signal transducer and activator of transcription (STAT), plays an important role in colorectal cancers (CRCs). Gefitinib (Gef) is an orally active inhibitor targeting the adenosine tri phosphate-binding domain of EGFR, and cucurbitacin B (CuB) is a selective inhibitor of JAK/STAT signaling with potent antitumor activity via suppression of STAT3 phosphorylation, but the underlying mechanism is not clear. We aimed to investigate the apoptotic and antiproliferative effects of CuB as a single agent and in combination with Gef on both HT-29 and HCT-116 cell lines. Cell proliferation, cell cycle distribution, and apoptosis were evaluated using viability assay, fluorescent microscopy, cytotoxicity assay, proliferation, DNA fragmentation, and cleaved caspase 3 levels. Real-time polymerase chain reaction and Western blot analyses were performed to determine the expression of relevant genes and proteins including antiapoptotic, proapoptotic, and cell cycle regulation. EGFR, phosphorylated EGFR (pEGFR), STAT3, and pSTAT3 proteins were evalutaed with Western blot analysis. Our results showed that, compared to CuB alone, CuB plus Gef treatment caused a significant growth and cell cycle inhibition and induced apoptosis in both cell lines. Also CuB plus Gef treatment decreased DNA synthesis rate more effectively than CuB alone. Treatment with CuB alone and in combination with Gef decreased the expression levels of B-Cell CLL/Lymphoma 2 (Bcl-2), BCL2-like 1 (BCL2L1), cyclin D1, pSTAT3, and pEGFR and increased the expression levels of Bcl-2-like protein 4, Bcl-2 homologous antagonist/killer, Bcl-2-associated death promoter, Bcl-2-like protein 11, and p27kip1 levels. Our results suggest that treatment with CuB alone and more likely in combination with Gef may be a considerable alternative therapeutic approach for CRC, at least in vitro.Gazi University Research FoundationGazi University [01/2011-56]The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported by Gazi University Research Foundation, with the project code number 01/2011-56
レクチン処理による骨性アルカリフォスファターゼ(ALP Ⅲ)の分離定量法
No full textFor decades, ALP had been interpreted as a marker enzyme of metabolic bone diseases and an isoenzyme specific for the osteoblast been widely known as ALP Ⅲ, too. Moreover, current tremendous advances in dialysis technology project an impact for the clinical significance of ALP Ⅲ, because of the advent of a new characteristic clinical entity formed renal osteodystrophy (ROD) which had been one of the inevitable complications in long term hemodialysis patients. So far, however, many conventional methods failed to separate clearly ALP Ⅲ from the liver specific ALP (ALP Ⅱ) and present determination of ALP Ⅲ by no means satisfied the clinical demand due to the lack of a direct quantitative method. On the other hand, lectin method, reported originally in 1984 by Rosalki, proved it to be possible to measure ALP Ⅲ directly as well as quantatively. So, the author performed this study to confirm more precisely several conditions of procedure of the lectin method, using established osteoblastic cell line ROS 17/2 as a marker, as well as the availability of its clinical application, and obtained the following results ; 1) The optimum concentration of Wheat-Germ lectin binded fully to ALP Ⅲ was 139 μmol/ml in distilled water. 2) The concentration of Triton X-100 preventing biliary ALP from ALP-lectin complex was 20%. 3) ALP Ⅲ activity incubated with lectin overnight at 4℃ after incubation for 30 min. at 37℃ was higher (mean±S.D, 24.0±15.2%) than that incubated with lectin for 30 min. at 37℃. 4) ALP Ⅲ was clearly separated from the liver fraction using affinity electrophoresis on polyacrylamide gel disc and isoelectric focusing on agarose gel. 5) ALP Ⅲ activity in precipitate showed good correlation (r=0.998) with the differential between total ALP activity and ALP Ⅱ activity in supernate.identifier:奈良医学雑誌 Vol.40 No.2 p.202-217identifier:04695550identifier:13450069identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/2281identifier:奈良医学雑誌, 40(2): 202-21
Balancing Diophantine triples with distance 1
Get PDFFor a positive real number let the Balancing distance be the distance from to the closest Balancing number. The Balancing sequence is defined by the initial values , and by the binary recurrence relation , . In this paper, we show that there exist only one positive integer triple such that the Balancing distances , and all are exactly 1.TUBITAK-BIDEBThis research is supported by TUBITAK-BIDEB. The paper was essentially prepared when the third author visited Department of Mathematics, Nigde University in August, 2013. The third author is grateful for the support of TUBITAK-BIDEB, and for the hospitality of Department of Mathematics, especially he is greatly indebted to Professor Murat Alp, Tarik Atay and Nurettin Irmak
The Return of Local Democracy: The CHP and the İstanbul Metropolitan Municipality
No full textThis article looks into the historical watershed of the 2019 local elections in Turkey and examines to what extent local government can empower opposition parties under the conditions of autocratic consolidation. The author looks into the case of the Metropolitan Municipality in Istanbul and argues that the administrative capacity-building and the cadre development, which the main opposition accomplished once in power can reinvigorate opposition politics and allow for the emergence of alternative power centres that may eventually challenge the incumbent
CAT1-POLYGROUPS AND PULLBACK CAT1-POLYGROUPS
Get PDFIn this paper, we give the notions of crossed polymodule and cat(1)-polygroup as a generalization of Loday's definition. Then, we define the pullback cat(1)-polygroup and we obtain some results in this respect. Specially, we prove that by a pullback cat(1)-polygroup we can obtain a cat(1)-group.TUBITAK-BIDEBThis research is supported by TUBITAK-BIDEB. The paper was essentially prepared during the first author's stay at the Department of Mathematics, Nigde University in 2012. The first author is greatly indebted to Professor Murat Alp for his hospitality and TUBITAK-BIDEB. Also, the authors are highly grateful to the referees for their valuable comments and suggestions for improving the paper
A reciprocating wear study on the effect of surfactant concentration and sliding speed in the electro codeposited Ni/SiCp metal matrix composites
Get PDFWOS: 000380280400002In this present work, Ni/SiC metal matrix composite coatings were prepared from a modified Watt's type electrolyte containing nano-SiC suspended particles by direct current plating method to increase the wear resistance of Ni. The influence of surfactant content on codeposition of SiC particles within the matrix and tribological properties were investigated. A wide particle size range (between 0.1 and 1.0 mu m) was chosen to provide a high load bearing ability for the codeposited layers. The wear tests were carried out at different sliding speeds by using a constant load. The influence of sliding speed on the tribological performances of the coatings has been investigated by using a reciprocating ball-on disk apparatus. Wear resistances and friction coefficients of Ni/SiC composites were decreased by increasing sliding speed due to temperature-controlled surface oxidation. The change in wear mechanisms by changing surfactant content and sliding speeds were also comprehensively studied.Scientific and Technical Research council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [106M253]The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work is supported by the Scientific and Technical Research council of Turkey (TUBITAK) under the contract number 106M253
Agarose gel electrophoresis of alkaline phosphatase isoenzymes in the serum of hyperthyroid cats
No full textCats with hyperthyroidism [(increased serum thyroxine (T(4))] commonly have increased serum alkaline phosphatase (ALP) activity in addition to other serum biochemical abnormalities. Serum biochemical profiles were obtained from 10 hyperthyroid cats which had increased serum ALP. Agarose gel electrophoresis of serum from these cats was performed and stained for alkaline phosphatase activity. Alkaline phosphatase activity was calculated for each of the separate bands obtained, and the results were compared to those of tissue extracts, serum from normal cats, and serum from normothyroid cats with increased serum ALP activity. The hyperthyroid cats had increased ALP activity in bands corresponding to isoenzymes originating in the liver, bone, and an unidentified tissue source.Source type: Electronic(1
230 s room-temperature storage time and 1.14 eV hole localization energy in In0.5Ga0.5As quantum dots on a GaAs interlayer in GaP with an AlP barrier
Get PDFThis article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. This article appeared in Appl. Phys. Lett. 106, 042102 (2015) and may be found at https://doi.org/10.1063/1.4906994.A GaP n+p-diode containing In0.5Ga0.5As quantum dots (QDs) and an AlP barrier is characterized electrically, together with two reference samples: a simple n+p-diode and an n+p-diode with AlP barrier. Localization energy, capture cross-section, and storage time for holes in the QDs are determined using deep-level transient spectroscopy. The localization energy is 1.14(±0.04) eV, yielding a storage time at room temperature of 230(±60) s, which marks an improvement of 2 orders of magnitude compared to the former record value in QDs. Alternative material systems are proposed for still higher localization energies and longer storage times
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