1,755,559 research outputs found
cDNA cloning and bacterial expression of an endo-β-1,4-mannanase, AkMan, from Aplysia kurodai
No full textPreviously we isolated an endo-β-1,4-mannanase (EC 3.2.1.78), AkMan, from the digestive fluid of a common sea hare Aplysia kurodai and demonstrated that this enzyme had a broad pH optimum spanning 4.0 to 7.5 and an appreciably high heat stability in this pH range (Zahura et al., Comp. Biochem. Physiol., B157, 137-148 (2010)). In the present study, we cloned the cDNA encoding AkMan and constructed a bacterial expression system for this enzyme to enrich information about the primary structure and the characteristic properties of this enzyme. cDNA fragments encoding AkMan were amplified by PCR followed by 5'- and 3'-RACE PCRs from the A. kurodai hepatopancreas cDNA using degenerated primers designed on the basis of partial amino-acid sequences of AkMan. The cDNA including entire translational region of AkMan consisted of 1,392 bp and encoded 369 amino-acid residues. The N-terminal region of 17 residues of the deduced sequence except for the initiation Met was regarded as the signal peptide of AkMan and the mature enzyme region was considered to comprise 351 residues with a calculated molecular mass of 39961.96 Da. Comparison of the primary structure of AkMan with other β-1,4-mannanases indicated that AkMan belongs to the subfamily 10 of glycosyl-hydrolase-family-5 (GHF5). Phylogenetic analysis for the GHF5 β-1,4-mannanases indicated that AkMan together with other molluscan β-1,4-mannanases formed an independent clade of the subfamily 10 in the phylogenetic tree. The recombinant AkMan (recAkMan) was expressed with an Escherichia coli BL21(DE3)-pCold1 expression system as an N-terminal hexahistidine-tagged protein and purified by Ni-NTA affinity chromatography. The recAkMan showed the broad pH optimum in acidic pH range as did native AkMan; however, heat stability of recAkMan was considerably lower than that of native enzyme. This may indicate that the stability of AkMan is derived from an appropriate folding and/or some posttranslational modifications in Aplysia cells
An endo-β-1,4-mannanase, AkMan, from the common sea hare Aplysia kurodai
No full textA mannan-degrading enzyme was isolated from the digestive fluid of the common sea hare Aplysia kurodai by ammonium sulfate fractionation followed by conventional column chromatography. The purified enzyme, named AkMan in the present paper, showed a single band with an approximate molecular mass of 40,000 Da on SDS-PAGE and preferably degraded a linear β-1,4-mannan from green algae Codium fragile producing triand disaccharides. The optimal temperature of AkMan was 55 °C at pH 7.0 and temperature that caused 50% inactivation of AkMan during a 20-min incubation was 52 °C. AkMan retained high activity at pH 4.0–7.5 and was not inactivated in such acidic pH range by the incubation at 40 °C for 20 min. AkMan could degrade glucomannan from konjak root and galactomannan (tara gum and guar gum) as well as the linear β-1,4- mannan, while not carboxymethyl cellulose, agarose, dextran and xylan. These results indicate that AkMan is a typical endo-β-1,4-mannanase (EC 3.2.1.78) splitting internal β-1,4-mannosyl linkages of mannan. The Nterminal and internal amino-acid sequences of AkMan shared ∼55% amino-acid identity to the corresponding sequences of an abalone β-1,4-mannanase, HdMan, which belongs to glycosyl hydrolase family 5 (GHF5). Thus, AkMan was also regarded as a member of GHF5 β-1,4-mannanases
GEOPARK INVENTORY STUDY OF LEVENT VALLEY, AKCADAG-MALATYA
No full textThe purposes of "Levent Valley Geopark Inventory Study" investigate each of many caves "which known to be over hundred" and study and record geological structures which could develop to become geosite both within the valley and the surrounding area. In Levent Valley -which is located at Levent, Akcadag village of Malatya-Lithostratigraphic units are surfaced dating back to Upper Cretaceous-Pliocene. The karstic caves found on the joints which is result of cross cutting of the faults of the main faults cutting through the valley, are mostly shaped by people who lived in the valley
Yeni pentadialdoz türevlerinin sentezleri
No full textBu tezin, veri tabanı üzerinden yayınlanma izni bulunmamaktadır. Yayınlanma izni olmayan tezlerin basılı kopyalarına Üniversite kütüphaneniz aracılığıyla (TÜBESS üzerinden) erişebilirsiniz.ÖZET YENİ PENTADİALDOZ TÜREVLERİNİN SENTEZLERİ SÜMER, Emel Yüksek Lisans Tezi, Kimya Anabilim Dalı Tez Yöneticisi: Prof. Dr. Levent YÜCEER Ağustos- 1999, 65 sayfa Bu tezde (S)-l,2-0-triWoroetiliden-a-L-araömo pentadialdozdan çıkılarak uronik asit türevleri sentezlenmiştir. Bu türevler (S)- 1,2-0- trikloroetiliden-a-L-arabinuronik asit, (S)-l,2-0-trikloroetiliden-3-0- asetil-a-L-arabinuronik asit, metil (S)-l,2-0-trikloroetiliden-a-L- arabinuronat, metil (S)-l,2-0-trikloroetiliden-3-0-asetil-a-L- arabinuronate ve metil (S)-l,2-0-trikloroetiliden-3-deoxi-3-en-ct-L- arabinuronat' lardır. Anahtar sözcükler: Uronik asitler, uronatlarVII ABSTRACT SYNTHESIS OF NEW PENTADIALDOSE DERIVATIVES SÜMER, Emel MSc. In Chemistry Supervisor: Prof. Dr. Levent YÜCEER August- 1999, 65 pages In this thesis uronic acid derivatives were synthesized starting from (S)-l,2-0-trichloroethylidene-a-L-araömo pentadialdose These derivatives are (S)-l,2-0-trichloroethylidene-a-L-arabinuronic acid, (S)- l,2-0-trichloroethylidene-3-0-acetyL-a-L-arabinuronic acid, methyl (S)- 1,2-0-trichloroethylidene-a-L-arabinuronate, methyl (S)- 1,2-0- trichloroethylidene-3-O-acetyL-a-L-arabinuronate, and methyl (S)-l,2- 0-trichloroethylidene-3-deoxy-3-ene-a-L-arabinuronate. Keywords: Uronic Acids, uronates
Yozgat-Sivas arasında kalan bölgenin vejetasyonunun (Geyikli Dağı, Sırıklı Dağı, Karababa Dağı, Ak Dağlar) sinekolojik yönden araştırılması
No full textAraştırma alanı İç Anadolu Bölgesinin doğu kesimini kapsamaktadır. Bitki coğrafyası bakımından Iran-Turan Floristik Bölgesinin Orta Anadolu sektöründe yer alır. Bölge yarı kurak alt ve üst çok soğuk ve son derece soğuk Akdeniz iklimlerinin etkisi altındadır. Araştırma alanının florası tür ve tür altı seviyede 843 takson içerir. Vejetasyon Braun-Blanquet (1932) metoduna göre araştırılmış olup yapılan çalışmalar neticesinde 10 bitki birliği ve 1 alt birlik tespit edildi. Tespit edilen birliklerden 4 tanesi ve 1 alt birlik bilim dünyası için yenidir. Birlikler ve bağlı oldukları üst birimler aşağıdaki gibidir. Ouercetea pubescentis Oberd. 1948 Ouerco-Carpinetalia orientali Akman, Barbero, Quezel, 1980. Carpino-Acerion Quezel, Barbero et Akman, 1980. Ouercion Anatolicae Akman, Barbero et Quezel, 1979. 1-Dorycnio - Pinetum sylvestrii ceraso-hieracietosum bornmuellerii KURT 2-Trifolio-Quercetum pubescentis 3-Ferulago-Quercetum cerridis 4-Cistetum laurifolii Astragalo-brometea Quezel, 1973 Onobrvchido-Thvmetalia Ieucostomi Akman, Ketenoğlu, Quezel, 1985Phlomido-Astragalion microcephali Akman et all. 1984. 5-Astragaletum microcephalo-compactii 6-Hyperico-Genistetum sessilifoliae KURT 7-Helichrryso-Astragaletum lagurii KURT 8-Astragaletum angustifolio-hausknechtii 9-Astragalo- Onohrydetum comutae KURT Asplenietea rupestria Br-BI. 1934 Silenetalia odontopetalae Quezel, 1973 Onosmion mutabile Quezel, 1973 10-Diantho engleri-Helichrysetum chinopylii KURTAbstractThe study area is located in the eastern part of Central Anatolia. From the Phytogeographic point of view, it is within the boundry of the Central Anatolian district of Iran-Turan Floristic region. The study area is under the influences of mediterranean climate with semi-aride,upper and lower cold, and very cold type. The Flora of the area consists of 843 species (inc. ssp. and var.). The vegetation of the area was situated according to Braun-Blanquet approach and classified into 10 associations and one subassociaton. Four of them and one subassociation are new for science. Associations and their higher units are as follows, Ouercetea pubescentis Oberd. 1948 Querco-Carpinetalia orientali Akman, Barbero, Quezel, 1980. Carpino-Acerion Quezel, Barbero et Akman, 1980. Ouercion Anatolicae Akman, Barbero et Quezel, 1979.1-Doronico - Pinetum sylvestrii ceraso-hieracietosum bornmuellerii KURT 2-Trifolio-Quercetum pubescentis 3-Ferulago-Quercetum cerridis 4-Cistetum laurifolii Astraealo-brometea Quezel, 1973 Onobrvchido-Thvmetalia leucostomi Akman, Ketenoğlu, Quezel, 1985 Phlomido-Astragalion microcephali Akman et all. 1984. 5-Astragaletum microcephalo-compactii 6-Hyperico-Genistetum sessilifoliae KURT 7-Helichrryso-Astragaletum lagurii KURT 8-Astragaletumangustifolio-hausknechtii 9-Astragalo- Onobrycetum cornutae KURT Asplenietea rupestria Br-Bl. 1934 Silenetalia odontopetalae Quezel, 1973 Onosmion mutabile Quezel, 1973 10-Diantho engleri-Helichrysetum chinopylii KUR
Erratum to "Total solidification time of a liquid phase change material enclosed in cylindrical/spherical containers" [Applied Thermal Engineering 25 (10) (2005) 1488-1502] (DOI:10.1016/j.applthermaleng.2004.10.005)
No full textRefers To
Levent Bilir, Zafer İlken
Total solidification time of a liquid phase change material enclosed in cylindrical/spherical containers
Applied Thermal Engineering, Volume 25, Issue 10, July 2005, Pages 1488-150
Besteci Necdet Levent ve Levent Müzik Evi
No full textAnkara : İhsan Doğramacı Bilkent Üniversitesi İktisadi, İdari ve Sosyal Bilimler Fakültesi, Tarih Bölümü, 2020.This work is a student project of the Department of History, Faculty of Economics, Administrative and Social Sciences, İhsan Doğramacı Bilkent University.The History of Turkey course (HIST200) is a requirement for all Bilkent undergraduates. It is designed to encourage students to work in groups on projects concerning any topic of their choice that relates to the history of Turkey. It is designed as an interactive course with an emphasis on research and the objective of investigating events, chronologically short historical periods, as well as historic representations. Students from all departments prepare and present final projects for examination by a committee, with 10 projects chosen to receive awards.Includes bibliographical references (page 13).İhsan Doğramacı Bilkent Üniversitesi'ndeki Hist 200 dersi için yapılan bu çalışmada, çok değerli bir sanatçı olmasına rağmen hak ettiği değeri göremediği düşünülen Necdet Levent araştırıldı. İlk olarak Necdet Levent'in hayatı araştırıldı ve müzisyenliğinde yaşam öyküsünün bir katkısı olup olmadığını incelendi. Daha sonra Levent'in müzisyen kişiliği araştırıldı. Bunun için kızından ve müzisyen arkadaşlarından bilgiler toplandı. Levent'in etkilendiği müzik akımları ve besteleri araştırıldı. Son olarak, Levent'in müziğe başladığı yıllardan beri açmayı hayal ettiği müzik evi araştırıldı. Bu müzik evinin içeriği ve İzmir'deki önemi araştırıldı. Bu çalışmayı yaparken kendisiyle geçmişte yapılan röportajlar, onun yakınlarıyla yapılan görüşmeler ve geçmişteki gazete haberleri yol gösterici olmuştur.In the study for the course Hist 200 (History of Turkey) in İhsan Doğramacı Bilkent University, it's been researched about Necdet Levent who was a worthy artist despite of the fact that he could not see the value of what he deserved. Firstly, Levent's life had been researched and it is investigated whether his life story has an effect on him being a musician. After that, it had been researched about Levent's musician personality and the information from his daughter and his musician friends gathered. Music trends that he might be affected and his compositions had been researched. Lastly, the music house which Levent had been dreamed about since the beginning of his musical carreer had been researched. Content of this music house and its importance for İzmir had been researched The interviews with Necdet Levent and with his relatives and the newspaper articles in the past were a guidance to the study.by Abdürrahim Özer
Levent Arşiray:Uzakdoğu-1
No full textTaha Toros Arşivi, Dosya No: 56-Levent Arşıray.
Not: Sergi, 18 Nisan - 15 Mayıs 1989 tarihleri arasında düzenlenmiştir
On the correct determination of rotational angles for twisted-profiled sweep objects
No full textIn the context of the paper of V. Akman and A. Arslan, "Sweeping with All Graphical Ingredients in a Topological Picturebook" [Computers & Graphics, Vol. 16(3), pp. 273-281, 1992], a new construction of the rotational matrix is presented. This fixes a bug discovered by the first author
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