2,178 research outputs found

    Phenotypic variation and molecular signaling in the interaction of the rhizosphere bacteria Acidovorax sp. N35 and Rhizobium radiobacter F4 with roots

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    The aim of this doctoral thesis was to investigate the factors relevant in plant interaction of two plant growth promoting rhizobacteria (PGPR). For this, the strain Acidovorax sp. N35 isolated from surface sterilized wheat roots and the two strains F4 and F7 of Rhizobium radiobacter, a bacterium associated with the plant growth promoting fungus Piriformospora indica, were chosen. First of all, the isolate N35 was characterized using phylogenetic and taxonomic methods. The 16S rRNA gene sequence analysis showed that strain N35 has the closest sequence similarities (98.2, 98.5 and 99.0 %) to the environmental Acidovorax species A. delafieldii, A. facilis and A. defluvii. The DNA-DNA hybridization values clearly separated the isolate from these three species. Additionally, phenotypic properties, such as substrate metabolization profiles as determined by a Biolog GN2 assay and cell wall fatty acid profiles concerning the fatty acids C16:0, C16:1ω7cis/trans, C17:0cyclo and C18:0cyclo and C19:0cyclo, facilitated the differentiation of the newly isolated strain N35 from its closest relatives. Thus, the strain N35 was classified as representative of a new species within the genus Acidovorax, and the name Acidovorax radicis sp. nov. is suggested. “Cand. A. radicis” N35 undergoes an irreversible phenotypic variation, resulting in different colony shapes on agar plate. In soil system, both phenotypes showed a plant growth promoting effect both on barley roots and shoots. The wild type N35 (rough colony type) had a better plant growth promoting effect on barley in comparison with phenotype variant N35v (smooth colony type). Wild type and phenotype variant cells of “cand. A. radicis” N35 were labeled with GFP and/or YFP and their separate and co-colonization behavior was investigated in a monoxenic system and a soil system using a CLSM for detection. Both types of N35 could endophytically colonize barley roots after 12 weeks inoculation in the soil system. Competitive root colonization behavior was observed after co-inoculation with differentially labeled wild type N35 and phenotype variant N35v bacteria, where the wild type showed dominant colonization of barley roots compared to the phenotype variant. Moreover, the variant N35v lost its motility due to missing flagella and swarming ability. The differences of both types at genetic level were investigated using whole genome sequence data obtained from 454 pyrosequencing (Roche) using the GS FLX Titanium chemistry. As only difference in the genome sequence, a 16 nucleotides deletion was identified in the mutL gene, which encodes for the mismatch repair protein MutL. In phenotype N35v, the frame shift caused by this deletion leads to the formation of a stop codon in the coding gene, resulting in a truncated MutL protein with a missing functional MutL C-terminal domain. This mutation occurred in exactly the same way in all investigated phenotype variants. These results suggest that MutL might be directly or indirectly responsible for the phenotypic variation in “cand. A. radicis” N35. Quorum sensing signaling molecules produced by “cand. A. radicis” N35 were identified using biosensors as well as Fourier transform ion cyclotron resonance - mass spectrometry (FT-ICR-MS) and ultra performance liquid chromatography (UPLC). Both types of “cand. A. radicis” N35 possess the same AraI/AraR quorum sensing system, which belongs to the LuxI/LuxR type. The two N35 phenotypes produced nearly the same amount of 3-OH-C10-HSL in the exponential growth phase. A co-inoculation experiment of AHL producing wild type N35 and a constructed AHL negative mutant N35 ΔaraI showed that wild type N35 had a dominant colonization behavior compared to the AHL negative mutant on barley roots in a monoxenic system. These data indicate that quorum sensing is involved in regulation of root colonization by “cand. A. radicis” N35. The second examined PGPR, R. radiobacter, which occurs naturally as endofungal bacterium in the plant growth promoting fungus P. indica, was demonstrated to colonize the surface of barley roots with fluorescence in situ hybridization (FISH) in a monoxenic system. The interaction of P. indica harboring R. radiobacter with other rhizobacteria was investigated using plate confrontation assays. Antibiotics and lipopeptides produced and excreted by the plant growth enhancing rhizobacterium Bacillus amyloliquefaciens FZB42 and the biocontrol rhizobacterium Pseudomonas fluorescens SS101 were shown to be responsible for the observed inhibition of P. indica by these bacteria. R. radiobacter F4 and F7 were able to synthesize a variety of oxo- and hydroxyl-C8- to C12-HSL compounds. In addition, both strains also produced coumaroyl-HSL when coumaric acid was supplied in the medium. The lactonase expressing transformants F4 NM13 and F7 NM13, which are the AHL negative phenotypes, abolished the lipase and siderophore activity. Considering this, quorum sensing influences the production of metabolites including lipase and siderophores in R. radiobacter F4 and F7. Further work should be directed to the question whether quorum sensing also plays a role in the interaction of the bacterium with fungus and/or plant

    Die Thränen des innigsten Dankes flossen bey dem Sarge des weyland Durchlauchtigsten Fürsten und Herrn, Herrn Ernst Friedrich Carl Herzogs zu Sachsen, Jülich, Cleve und Berg ... welcher in der Nacht vom 22sten bis 23sten September 1780. im 54. Jahre Dero Höchstrühmlichen Alters im HErrn seelig entschliefe, von Höchstderoselben unterthänigsten Dienern und Unterthanen Johann Matthaeus Lotz, Amtmann. Friedrich Ludwig Albrecht, D. Med. u. Amtsphysicus ord. Johann Carl Germann Faber, Amtsadjunctus. Johann Friedrich Carl Kessel. Amtsverwalter. Johann Friedrich Carl Faber, Amtscopist. wie auch von den sämtlichen Schultheißen und Gemeinden des Herzogl. Amts Sonnenfeld

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    DIE THRÄNEN DES INNIGSTEN DANKES FLOSSEN BEY DEM SARGE DES WEYLAND DURCHLAUCHTIGSTEN FÜRSTEN UND HERRN, HERRN ERNST FRIEDRICH CARL HERZOGS ZU SACHSEN, JÜLICH, CLEVE UND BERG ... WELCHER IN DER NACHT VOM 22STEN BIS 23STEN SEPTEMBER 1780. IM 54. JAHRE DERO HÖCHSTRÜHMLICHEN ALTERS IM HERRN SEELIG ENTSCHLIEFE, VON HÖCHSTDEROSELBEN UNTERTHÄNIGSTEN DIENERN UND UNTERTHANEN JOHANN MATTHAEUS LOTZ, AMTMANN. FRIEDRICH LUDWIG ALBRECHT, D. MED. U. AMTSPHYSICUS ORD. JOHANN CARL GERMANN FABER, AMTSADJUNCTUS. JOHANN FRIEDRICH CARL KESSEL. AMTSVERWALTER. JOHANN FRIEDRICH CARL FABER, AMTSCOPIST. WIE AUCH VON DEN SÄMTLICHEN SCHULTHEISSEN UND GEMEINDEN DES HERZOGL. AMTS SONNENFELD Die Thränen des innigsten Dankes flossen bey dem Sarge des weyland Durchlauchtigsten Fürsten und Herrn, Herrn Ernst Friedrich Carl Herzogs zu Sachsen, Jülich, Cleve und Berg ... welcher in der Nacht vom 22sten bis 23sten September 1780. im 54. Jahre Dero Höchstrühmlichen Alters im HErrn seelig entschliefe, von Höchstderoselben unterthänigsten Dienern und Unterthanen Johann Matthaeus Lotz, Amtmann. Friedrich Ludwig Albrecht, D. Med. u. Amtsphysicus ord. Johann Carl Germann Faber, Amtsadjunctus. Johann Friedrich Carl Kessel. Amtsverwalter. Johann Friedrich Carl Faber, Amtscopist. wie auch von den sämtlichen Schultheißen und Gemeinden des Herzogl. Amts Sonnenfeld ([1]) Cover ( - ) Titelseite ([1]) Text ([2]

    Reconstructing Beethoven: Mauricio Kagel’s Ludwig van

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    The subject of this dissertation is Ludwig van, Mauricio Kagel’s tribute to Beethoven on the occasion of the 200th anniversary of the latter’s birth, which consists of three separate, but closely related, versions: a film, a musical score and a recording. The main aim of this project is to analyse the performance problems that musicians have to face when realising the score Ludwig van, which is an entirely indeterminate collage of Beethoven’s music, and to suggest ways of tackling them. For this purpose, all three versions of the work are studied in this thesis. The film is examined in terms of the issues it raises concerning Beethoven’s reception and of the function of its music, which consists of unusual performances of Beethoven’s works. The score is analysed from the perspective of postmodern theory and 20th-century art movements, while the roles of the composer and the performer are discussed and redefined. The recording is studied as a sample of how Kagel himself chose to realise his own score. Finally, the difficulties I encountered in my own attempts to realise Ludwig van are discussed, and the ways in which I dealt with them are presented. The conclusion at which this dissertation arrives is that, in works of such indeterminacy as Ludwig van, the performers are required to step outside their conventional role and act partly as composers. Compared to works that are considered challenging to the performer in the conventional sense, of requiring technical virtuosity, this work presents a more fundamental challenge, which has to do with overcoming personal boundaries: it asks the performer not to execute a pre-composed work, but to create their own version of Ludwig van. Since very little has been written about Ludwig van by performers with an academic background, this thesis can offer valuable assistance to prospective performers of the work in their attempt to balance between the highly charged conceptual aspect of the composition and the practical need to achieve its successful performance

    The birth of the (non) European author

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    The Birth of the (Non)European Author draws attention to the power relations between subaltern authors and the field of publishing, especially focusing on the ownership of author rights and the representation of authorship. By particularly concentrating on the creation and marketing of "indigenous authorship" and "marginality" as goods on the global book market, the relationship between "subaltern/marginalised" authors and agents of the book market, such as editors and publishers is highlighted as unbalanced and precarious. This book traces the genre testimonial narration, from slave narratives to African-American child soldier memoirs, analysing the relationship of Western publishing modes and what is being sold as “African authorship”. Combining explorations of theories of representation and authorship with close readings of testimonial narrations and the analysis of the relationship between professional writer and witness, this book contributes to the field of postcolonial theory, globalization studies and book history. Anna-Katharina Krüger studied Comparative Literature at the Ludwig-Maximilians University in Munich and Book History at the Oxford Brookes University. As a scholar of the DFG research training group "Globalization and Literature - Representations, Transformations, Interventions" she finished and defended her dissertation in 2017. Her research focus lies on postcolonial literature, authorship and representation theory as well as testimonial narration

    Ludwig-Maximilians-Universität München: What does a university look like?

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    Ludwig-Maximilians-Universität München About the Researchers The network above represents a connected graph of 15,558 co-authored researchers affiliated to the Ludwig-Maximilians-Universität München from 2017-2022, making up 91% of all affiliated researchers over this time period. Each researcher has been colour coded by the 2-digit FoR 2020 code they are most associated with. Each researcher is depicted by a sphere, and given a size based on the number of publications produced. About the Clusters 181 research clusters were identified in the network above. To make the network easier to read, collaborations between clusters are not displayed, although they do play a significant role in the layout of the network. Clusters of 20 or more researchers can be explored further in the associated figshare record (linked in the QR code top right of legend). Clusters are colour coded by the most dominant discipline of the researchers within them, and are given a ‘height' based on the discipline that they proportionally belong to. Biomedical and Clinical Sciences clusters sit at the base of the network, with Language, Communication and Culture sitting at the top. About the Classifications The 2020 Field of Research codes used in this analysis have been assigned to publications using the approach detailed in “Recategorising research: Mapping from FoR 2008 to FoR 2020 in Dimensions” (https://doi.org/10.1162/qss_a_00244.) Note: some research areas are not well represented in the network due to single author publications. Fields of Research with greater than 50% of their output not represented in the network include: Mathematical Sciences (57.6%), Human Society (59.58%), Commerce, Management, Tourism and Services (62.41%), Economics (63.91%), Language, Communication and Culture (65.52%), History, Heritage and Archaeology (69.95%), Law and Legal Studies (70.78%), and Philosophy and Religious Studies (82.05%) Methodology: Graph layout: Batchlayout [1] Clustering: Leiden Algorithm [2] 3d Layout: Blender [3] Data: Dimensions [4]</p

    Das Landgut Monrepos in Finnland. 1804

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    Author: Ludwig Heinrich von Nicolay.Digital reproduction, The National Library of Finland, Centre for Preservation and Digitisation, MikkeliA poetic description of the manor and the park of Monrepos in Vyborg, owned by Ludwig Heinrich von Nicolay.TravelEuropeanaNicolay, Ludwig Heinrich von (1737-1820

    Ludwig Feuchtwanger Collection 1908-1973

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    Correspondence with individuals, including Alexander Altmann, Werner Cahnmann, Guido Kisch, Raphael Straus, and Max Warburg; business correspondence with publishers and organizations; correspondence with family members, including his brother, the novelist Lion Feuchtwanger.Manuscripts by Feuchtwanger on various topics, including the Jewish Question and history and sociology of the Jews; clippings by and about Feuchtwanger; and photos.Curricula vitae, bibliographies, professional documents, and material relating to Feuchtwanger's search for employment in Great Britain, including letters of recommendation from Leo Baeck, Leo Baerwald, and Martin Buber.Manuscripts by other individuals, including Bertha Badt-Strauss, Chaim Bloch, Werner Cahnmann, Dora Edinger, Georg Hermann, Hans Kohn, and Nelly Sachs.Field letters from the Verein Mekor Chajim, an orthodox group affiliated with the Israelitische Religionsgesellschaft of Frankfurt am Main, to Jewish soldiers during World War I; sermons from the Nazi period by various rabbis, including: Alexander Altmann, Joseph Carlebach, Max Eschelbacher, Moses Hoffmann, Jakob Horovitz, Alfred Jospe, Max Kapustin, Emil Levy, Siegmund Maybaum, Hermann Schreiber, Caesar Seligmann, and Hermann Vogelstein.The following individuals are mentioned in this collection:Altmann, Alexander; Badt-Strauss, Bertha; Baeck, Leo; Baer, Erwin; Baerwald, Leo; Baumgarten, Beate; Ben-Chorin, Shalom; Ben-Gavriel, M.Y.; Bialik, Chaim Nachman; Blau, Ernst; Bloch, Chaim; Bloch, Olga; Blumenfeld, Walter; Bornstein, Paul; Buber, Martin; Caesar, Egon; Cahnmann, Werner; Carlebach, Joseph; Cohen, Carl; Cohn, Willie; Doernberg, Erwin; Edinger, Dora; Eisenstaedter, Julius; Ernst, Rudolf; Eschelbacher, Max; Essrog, Chaim; Feuchtwanger, Lion; Flank, Joseph; Fraenkel, Fitz Meir; Friedlaender, Fritz; Fuerstenthal, Ernst; Gallinger, Arthur; Glaser, Siegfried; Gundersheimer, Hermann; Guttmann, Julius; Hepner, Isi; Hermann, Georg; Herz, Reinhold; Hirsch, Siegmund; Hirschfeld, Georg; Hoffmann, Moses; Homburger, Hanna; Horovitz, Jakob; Joachimsthal-Schwabe, Anna; Jospe, Alfred; Kapustin, Max; Katten, M.; Kisch, Guido; Kohn, Hans; Lamm, Hans; Lamm, Louis; Leisegang, Hans; Lemkowitz, Albert; Lemle, Heinrich; Leuner, Leo; Levi, Julius Walter; Levy, Emil; Lichtenstein, Erich; Loewe, Fritz; Loewe, Heinrich; Loewenthal, Ernst; Ludwig, Elly; Mainz, Annie; Martius, Adam; Marx, Hilde; Maybaum, Siegmund; Mayer, Karl; Mayer, Ludwig; Petuchowski, Ernst; Posen, Ida; Prinz, Joachim; Rabinowitz, Sally; Reichberger, Arthur; Rothschild, Lothar; Sachs, Nelly; Schach, Fabius; Schilcher, Johann; Schmitt, Rudolf; Schreiber, Hermann; Schuster, M.; Schwarz, Martin; Seligmann, Caesar; Siegel, Emes; Sinsheimer, Hermann; Steckelmacher, Ernst; Steil, Moses; Straus, Raphael; Sturmann, Manfred; Tramer, Hans; Untermeyer, Max; Vogelstein, Hermann; Warburg, Max; Wassermann, Rudolf; Wechselmann, Ernst; Weinberg, Josef; Weltmann, Lutz; Wertheimer, Martha; Wittelshofer, Fritz; Wittenberg, ErichSee Inventory listBorn in Munich on November 28, 1885, Ludwig Feuchtwanger was trained as a lawyer, but worked as an author, journalist, and publisher. He was editor of the publishing house Duncker & Humbolt and lecturer at the "Mittelstelle der juedischen Erwachsenenbildung." He emigrated to Great Britain in 1939, where he was briefly interned on the Isle of Man. He was a lecturer at the "Jewish Historical Society of England" and died in Winchester, Great Britain, on July 14, 1947.16-page inventory for Series I-VIIdigitize

    Geschichte eines Hottentottens

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    The original document was digitized with financial support from Media24.Door hem zelven beschrevenThe author is Christian Ludwig Willebrandhttp://explore.up.ac.za/record=b171616

    Schlacht am Morgarten am Zuger-Gebiet, so geschehen a[nn]o 1315

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    [Heinrich Ludwig Muoss]Aus: Heinrich Ludwig Muoss: Helvetia, Rhaetia, Valesia. Es handelt sich um einen Ausschnitt aus der Randleiste der zweiten Auflage (Zug, beim Author, 1710) oder der dritten Auflage (Zug, bei Joh. Jost Hiltensperger, 1770)
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