1,724,918 research outputs found
Effects of miR-5195-3p overexpression on ARPE-19 cell viability, inflammation and apoptosis after HG stimulation.
No full text(A) Analysis of reverse transcription quantitative PCR was conducted to evaluate miR-5195-3p expression in ARPE-19 cells transfected with miR-5195-3p mimics or miR-NC. ***p p p p < 0.01, compared with HG + miR-NC; NC, negative control; HG, high glucose; NG, normal glucose.</p
The expression levels and correlation of miR-5195-3p and GMFB.
No full text(A) In HG-induced ARPE-19 cells, miR-5195-3p was significantly downregulated compared with that of NG group. (B) GMFB protein expression was upregulated in HG-induced ARPE-19 cells compared with that of NG group. ***p p < 0.01 compared with miR-NC. NC, negative control; HG, high glucose; NG, normal glucose; WT, wild type; MUT, mutant type.</p
Knockdown of GMFB enhanced the protective effects of miR-5195-3p overexpression against HG-induced ARPE-19 cell injury.
No full textARPE-19 cells were co-transfected with miR-5195-3p mimics and si-GMFB or si-NC, and then treated with HG for 24 h. (A) The protein expression of GMFB was detected by western blot analysis. (B) Cell viability was analyzed using CCK-8 assay. (C-D) ELISA assay was performed to analyze the release of IL-1β and TNF-α in transfected ARPE-19 cells, followed by HG stimulation. (E) The percentages of apoptotic cells were compared in transfected ARPE-19 cells, followed by HG stimulation. Data were shown as mean ± SD. *p p p p < 0.01, compared with HG + miR-5195-3p mimics + si-NC; NC, negative control; HG, high glucose; si, small interfering.</p
Overexpression of GMFB reversed the protective effects of miR-5195-3p overexpression against HG-induced ARPE-19 cell injury.
No full textARPE-19 cells were co-transfected with miR-5195-3p mimics with either pcDNA3.1-GMFB or pcDNA3.1, followed by 24 h incubation with HG. (A) The protein expression of GMFB was detected by western blot analysis. (B) Cell viability was analyzed using CCK-8 assay. (C-D) ELISA assay was performed to analyze the release of IL-1β and TNF-α in transfected ARPE-19 cells, followed by HG stimulation. (E) The percentages of apoptotic cells were compared in transfected ARPE-19 cells, followed by HG stimulation. Data were shown as mean ± SD. *p p p p p < 0.001, compared with HG + miR-5195-3p mimics + pcDNA3.1; NC, negative control; HG, high glucose.</p
MiR-5195-3p functions as a tumor suppressor by targeting RHBDD1 in ovarian cancer
Get PDFBackground. Recent studies have reported the tumor suppressive role of miR-5195-3p in the progression of several cancers, but the potential roles of miR-5195-3p in ovarian cancer (OC) remain largely unknown. Methods. We first analyzed the expression levels of miR-5195-3p in 83 pairs of human OC tissues and adjacent specimens by reverse transcription-quantitative PCR. The correlation of miR-5195-3p/rhomboid domain containing 1 (RHBDD1) and clinicopathological parameters was analyzed by chi-square test. The prognostic value of miR-5195-3p was evaluated by Kaplan-Meier method Cox proportional hazards models. The effects of miR-5195-3p on cell proliferation, cell cycle distribution, migration and invasion were examined by CCK-8 assay, colony formation assay, flow cytometry and transwell assay. Tumor forming was evaluated by nude mice model in vivo. The association between miR-5195-3p and RHBDD1 was verified by luciferase reporter assay. Results. We observed that miR-5195-3p level was remarkably reduced in OC tissues as compared to adjacent tissues. The expression of miR-5195-3p was associated with FIGO stage, depth of invasion and poor survival prognosis in OC patients. Overexpression of miR-5195-3p significantly suppressed cell proliferation, cell cycle G1/S transition, migration and invasion in OC cell lines (SKOV-3 and OVCAR3), while knockdown of miR-5195-3p obtained the opposite results. We further confirmed miR-5195-3p as a negative posttranscriptional modulator of RHBDD1. RHBDD1 expression was upregulated in OC tissues compared with adjacent tissues, which was inversely correlated with miR-5195-3p expression. The expression of RHBDD1 was associated with FIGO stage and distant metastasis. RHBDD1 overexpression reversed the suppressive role of miR-5195-3p on OC cell proliferation, migration and invasion. Consistent with the in vitro results, miR-51953p overexpression decreased the growth of subcutaneously inoculated tumors in nude mice. Conclusions. Taken together, the present results indicated that miR-5195-3p acts a tumor suppressor by targeting RHBDD1 in OC
After the interaction: An efficiently star-forming molecular disk in NGC 5195
Get PDFWe present new molecular gas maps of NGC 5195 (alternatively known as M51b) from the Combined Array for Research in Millimeter Astronomy, including 12CO(1-0), 13CO(1-0), CN(1-), CS(2-1), and 3 mm continuum. We also detected HCN(1-0) and HCO+(1-0) using the Onsala Space Observatory. NGC 5195 has a 12CO/13CO ratio (R12/13= 11.4 \ub1 0.5) consistent with normal star-forming galaxies. The CN(1-0) intensity is higher than is seen in an average star-forming galaxy, possibly enhanced in the diffuse gas in photo-dissociation regions. Stellar template fitting of the nuclear spectrum of NGC 5195 shows two stellar populations: an 80% mass fraction of old (10 Gyr) and a 20% mass fraction of intermediate-aged (?1 Gyr) stellar populations. This provides a constraint on the timescale over which NGC 5195 experienced enhanced star formation during its interaction with M51a. The average molecular gas depletion timescale in NGC 5195 is = 3.08 Gyr, a factor of larger than the depletion timescales in nearby star-forming galaxies, but consistent with the depletion seen in CO-detected early-type galaxies. While radio continuum emission at centimeter and millimeter wavelengths is present in the vicinity of the nucleus of NGC 5195, we find it is most likely associated with nuclear star formation rather than radio-loud AGN activity. Thus, despite having a substantial interaction with M51a ?1/2 Gyr ago, the molecular gas in NGC 5195 has resettled and is currently forming stars at an efficiency consistent with settled early-type galaxies
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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