Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter
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Survey Of The Cardiovascular Drug Interactions On Geriatrics At Internal Medicine Ward Of RSUDZA Banda Aceh
No full textThe increase of elderly population in Indonesia leads to the drug misuse. The physiological change in geriatric become the major reason. It affects the change of drugs pharmacodynamic, pharmacokinetics and the harmful drug interaction among overly prescribed medication. Cardiovascular drugs produce the most common side effect in elderly patient. This study aims to observe the accuracy of cardiovascular drugs used based on its dose and drug interaction. The subject of this study are the 60 years old-or more- inpatients in Rumah Sakit Umum Zainoel Abidins (RSUDZA) Internal medicine ward who received cardiovascular drugs prescription. The data were collected from medical record and nurses drugs record. The analysis were performed descriptively by evaluate the drug interaction theoritically based on literature study and available concensuses. The result shows that the Potential interaction occurred in 75 ( 68.18 % ) of cardiovascular drug used, and 22 (20 %) of whom are important potential interactions. The most frequent drugs that have the potential interaction in this study are furosemide , spironolactone, captopril, digoxin and aspiri
Some Rice Varieties Tolerant to Nitrogen Deprivation
No full textA research on rice varieties tolerant to nitrogen deficiency has been conducted. The experiment was arranged in a split plot design with 3 replicates. There were 15 varieties of rice studied and 2 levels of nitrogen applied, i.e. 0 kg Nha-1 and 90 kg Nha-1. The results showed that rice varieties resistant to nitrogen deficiency were Inpari 16, Marga Sari, Lambur, Inpari 19, Limboto, and Batang Hari. However, rice varieties with high-tolerant to nitrogen deficiency were Marga Sari, Inpari 16, Lambur, Batang Hari, and Situ Patenggan
IL-12 PE, CD 69 PERCP, CD3 FITC, AND CD4 APC OPTIMIZATION WITH ACTIVATION OF ISOLATED AGENT HEAT-KILLED SONICATED MYCOBACTERIUM TUBERCULOSIS BEIJING STRAIN
No full textInfection caused by Mycobaterium tuberculosis exists in form of intracellular infection, which leads to lymphocyte activation. CD69 is the first lymphocyte activation marker expressed in Th1 lymphocyte, which follows by IL-12 release. Flow cytometry analysis can identify the subpopulations of lymphocytes and intracellular cytokines such as IL-12, yet precise preparation needs to be done. This research aims to conduct optimization with four color lyse/wash flow cytometry assay system FastImmune FACSCalibur examination, with monoclonal antibody IL-12, CD69, CD3, and CD4 in succession uses fluorochrome PE, PerCP, FITC, and APC.To activate the lymphocytes from heparinized whole blood, we used activation agent which derives from isolated heat-killed sonicated Mycobacterium tuberculosis Beijing strain. Optimal concentration from the according activation agents is 40 mL. To determine the compensation, BDTM CompBead and blank-cell unstainning are used, but the maximum result showed by blank-cell unstainning.Each monoclonal antibody dosage of IL-12PE, CD69 PerCP, and CD3 FITC is 40 mL, while CD4 APC 5 mL. Total event lymphocyte is determined minimally by 10,000 events. With 18,510 total events and Th gated events quantity are 4,692, the result obtained is IL12-PE has 7.4% gated (347 events); CD69+ perCP/CD3+ FITC 18.2% (850 events); and CD69+ perCP/CD4+ APC 3.9%
PREPARATION OF ANTI-IDIOTYPE ANTIBODY AS DIAGNOSTIC KIT IN RABIES SEROLOGICAL TEST
No full textSerology remains the only way to monitor the effectiveness of vaccination of humans and animals against rabies. Many techniques for determining the level of rabies antibodies have been described, and all of the methods used rabies viruses as antigen. Indeed attenuated viruses can revert to a more virulent form, and inactivated virus may produce serious side effects. Anti-idiotype Ab can induce protective immune response against rabies virus, its means that anti-idiotype Ab can be used as surrogate antigen in serological test.The aim of this study is to prepare the anti-idiotype antibody (anti-idiotype Ab) as diagnostic kit in rabies serological test. Polyclonal anti-idiotype Ab were prepared in laying chickens and purified using affinity chromatography column for IgY. Rabbit anti dog immunoglobuline were prepared used New Zeeland White strain, and dog anti rabies serum were prepared from rabies immunized dog. For the preparation of the kit, it takes a few stages, i.e. the making of stocks A, stocks B and prototype diagnostic kit. Stocks A is a mixture of S. aureus Cowan I intact in solution of Tris Buffer HCl with rabbit anti dog serum in various comparisons (v/v). Stocks B is a mixture of rabies anti-idiotype Ab with rabies antibody (IgG anti rabies) are harvested from dog.Comparison of the obtained between whole S. aureus Cowan I and rabbit serum anti dog is 4~6:2~4. Optimization of stocks A and B based on the principle as follows: a merger of stocks A and B must not cause coagglutination. Formulation of aqueous stocks A and B will be a candidate when the diagnostic kits on the positive control produces coagglutination and negative controls do not produce coagglutination. The conclusions of this study is the anti-idiotype antibody can used and prepared as kit diagnostic with the principle of coagglutination by utilizing A protein of S. aureus
The Correlation Between Level of Diabetic Patients Knowledge with Quality of Life in Patients with Type 2 Diabetes Mellitus at Haji Adam Malik Hospital Medan
No full textDiabetic complications is usually asssociated with low quality of life in diabetic patients. Knowledge of diabetes become an important role in the management of people with diabetes, at least in self-care to prevent further complication. With a better knowledge about diabetes, people with diabetes will have better self-care in diabetic treatment in order to have better quality of life. This study aimed to evaluate the correlation between the level of diabetic patients knowledge and quality of life in patients with type 2 Diabetes Mellitus at Haji Adam Malik Hospital Medan. Ninety subjects were recruited by consecutive sampling technique at outpatients of Endocrinology and Metabolism clinic, Internal Medicine Department Haji Adam Malik Hospital. The level of knowledge about diabetes was assessed by using validated questionnaires, and quality of life was assessed by using the WHOQOL-BREF questionnaire. Pearson correlation was used for statistical analysis. Results showed that majority of patients have a average level of diabetes knowledge (42,2%). Quality of Life was measured by four domains based on the WHOQOL-BREF. The overall quality of life shows that majority of patients have average level quality of life (50%). In the domain of physical health, 65.6% patients have poor level quality of life. Majority of patients have good level quality of life in the domain of psycology (48.9%), social relationships (42.2%) and environment (50%). There was positive correlation between level of knowledge and quality of life (r = 0.239, p0.005). Conclusion This study showed that better knowledge of diabetes could improve quality of life of patients with type 2 diabetes. It is recommended that diabetic patients should improve their understanding about diabete
Screening of Degenerative Diseases and Quality of Life among Elderly People in Posyandu Lansia Medan Amplas Sub-Districts, Medan
No full textThe number of elderly people in Indonesia from year to year is increasing in line with increasing life expectancy. The number of elderly people reached 7.56% of the population. Certainly, the increase in the elderly population will be followed by an increased risk for the elderly to suffer from degenerative diseases. This study aimed to assess the role of degenerative disease screening and health counseling to the quality of life of the elderly in the health post (Posyandu Lansia). This study was a descriptive analytic with prospective approach by assessing 100 elderly people. Screenings done for examination of blood pressure, blood sugar level, cholesterol level, uric acid level, and urine protein. Assessment of quality of life done by using questionnaire of WHOQOL. The elderly in Posyandu Lansia were educated in accordance with the results of the screening and followed up for 4 months. Screening results showed that most elderly people had high blood pressure (89% had high systolic blood pressure and 70% had high diastolic blood pressure). As much as 55% of elderly people had cholesterol levels 200 mg/dl, while blood sugar levels as 200 mg/dl were 20% of the elderly and only 20% of respondents who had uric acid levels that exceed normal limits (8,5mg/dl). As many as 13 of respondents indicated a positive result in urine protein. Assessment of the quality of life of elderly based on the score at first examination obtained a mean of 88.26 9.1. Regarding general health status, 21% of elderly felt less satisfied, 47% declared that their health status in normal conditions and 32% felt satisfied with their current health status. The score of quality of life after receiving counseling, obtained a mean of 89.31 7.8. Assessment of general health status showed only 11% of elderly declared unsatisfactory health status. Result of t test showed that there was a significant difference between score of quality of life before and after screening degenerative diseases (p0.05). Screening of degenerative diseases showed a majority of the elderly had a potential risk to suffer from degenerative diseases. Prevention through screening and health counseling particularly in Posyandu Lansia can reduce morbidity and mortality in elderly, and therefore it will improve quality of life for the elderl
Enhance the Grouth and Flowering of Roses (Rosa galica L.) Due to Composted Waste Coffee Powder and Gibberellins Concentration
No full textThis study aims to determine the effect of composted waste coffee powder and gibberellins concentration on the growth and flowering of roses (Rosa galica L.) as well as the interaction between the two factors. Research conducted at the Experimental Farm of the Faculty of Agriculture Syiah Kuala University, Banda Aceh. This study used a Randomized Block Design (RAK) in factorial arrangement; there are two factors with three replications. The first factor is composted waste coffee powder consisting of: soil + composted waste coffee powder (1:1), soil + composted waste coffee powder (1:2), sand + composted waste coffee powder (1:1), sand + composted waste coffee powder (1:2). The second factor is the concentration of gibberellins consisting of: 0, 100, 200, and 300 ppm. The results showed that the composition of the growing media composted waste coffee powder has no significant effect on all parameters observed. Planting media composition tends to be better found in sand + composted waste coffee powder (1:1). The concentration gibberellin very significant effect on the number of branches at the age of 60 days after planting (DAP), significant effect on plant height, stem diameter and number of rose plants flower, at the age of 45 and 60 DAP. The best growth and flowering of roses was found on concentration of 200 ppm gibberellin. There was no significant interaction between composted waste coffee powder with gibberellin concentration on all parameters observe
Production and Physiological Characters of Soybean Varieties Under Drought Stress with Application of Nitrogen Sources
No full textSoybean is a legume sensitive to drought conditions resulting in decreased yield and seed quality. Drought stress on plants also inhibits nitrogen uptake. The objective of the research was to determine production and physiological characters of soybean varieties under drought stress through nutrient N management. This research used a randomized block design with 3 factors and 3 replications. The first factor was soybean varieties (Anjasmoro, Wilis and Sinabung). The second factor was drought stress conditions consisted of 50%, 60% , 70% and 80% of field capacity (FC). The third factor was the application of N consisted of (1). Without application of N fertilizer (control); (2). N fertilizer dose of recommendation (50 kg Urea/ha); (3). Inoculation of Bradyrhizobium sp. ; (4). Organic N sources (straw compost 10 tons/ha) ; (5). Organic N sources (farmyard manure 10 tons/ha). The results suggest that Anjasmoro variety improved dry weight of seed per plant compared with Willis and Sinabung. Increased drought stress ( 80-50 % of FC) resulted in a decrease in dry weight of seed per plant. Sources of N in the form of urea or Bradyrhizobium sp. increased the dry weight of seeds per plant compared with treatment N sources straw and manure compost. The interaction between Bradyrhizobium sp. or Urea and Anjasmoro variety improved dry weight of seeds per plan
Analysis of Factors Affecting The Quality of Life in Vitiligo Patients at Dermato-Venereology Polyclinic dr. Zainoel Abidin General Hospital, Banda Aceh
No full textVitiligo is a skin depigmentation disorder and chronic asymptomatic due to the destruction of melanocytes. Patients with vitiligo in addition to physical disorders also affect the psychological state that disrupts the quality of life of the patients. This study aims to identify the factors that affect the quality of life of vitiligo patients. Analytic study was conducted using cross-sectional design with questionnaire Dermatology Life Quality Index (DLQI) to vitiligo patients who went to the Dermato-Venereology Policlinic dr. Zainoel Abidin General Hospital, Banda Aceh. The results of this study found that visible location and long-suffering vitiligoless than one year affect the quality of life of patients (p0.05) which the duration of suffering vitiligomore influence than vitligo location
Inhibition of Salmonella sp. Growth in Comparison with Antibiotics and Pineapple (Ananas comosus) Squeeze
No full textThe purpouse of this study to examine antibiotics inhibition growth of Salmonella sp. isolated from feces of broiler chickens to chloramphenicol, gentamicin, and tetracycline in coparison with pineapple squeeze. Swab sample from broiler chicken cloaca was cultured to nutrient broth media and incubated at 37C temperature for 24 hours. Culture was compared with Mc Farland 3 standardization, and separated on surface of Muller Hinton Agar (MHA). Antibiotic sensitivity was tested by mean of chloramphenicol, gentamisin, and tetrasiklin in coparison with pineapple squeeze. The antibiotic disks (Oxoid) were put on the surface of MHA media which had inoculated with Salmonella sp. bacteria. Antibiotic sensitivity was determined based on formation of inhibition zone in surrounding of antibiotic disc. The result showed that Salmonella sp. bacteria growth was inhibited in 21 mm and 18 mm, 7 mm and 9 mm against chloramphenicol, gentamisin, tetrasiklin, and pineapple squeeze respectively. This research concluded that the Salmonella sp. cultured in this manner were sensitive to chloramphenicol and gentamisin. The inhibition growth of Salmonella sp. caused by chloramphenicol and gentamicin was significantly higher in comparison with pineapple (Ananas comosus) squeeze, meanwhile the Salmonella sp. bacteria was resistance against tetracycline. Our result suggest that both antibiotics chloramphenicol and gentamicin were recommended for antibiotic therapy in Salmonella sp. infection